Amino acid dipepetide frequency for bacterium HR25

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.016AlaAla: 14.016 ± 0.295
1.091AlaCys: 1.091 ± 0.063
4.618AlaAsp: 4.618 ± 0.133
7.336AlaGlu: 7.336 ± 0.169
3.412AlaPhe: 3.412 ± 0.122
10.071AlaGly: 10.071 ± 0.202
2.234AlaHis: 2.234 ± 0.098
5.007AlaIle: 5.007 ± 0.133
2.016AlaLys: 2.016 ± 0.086
15.25AlaLeu: 15.25 ± 0.3
2.455AlaMet: 2.455 ± 0.094
1.592AlaAsn: 1.592 ± 0.079
5.323AlaPro: 5.323 ± 0.169
4.331AlaGln: 4.331 ± 0.141
11.361AlaArg: 11.361 ± 0.234
5.232AlaSer: 5.232 ± 0.163
4.611AlaThr: 4.611 ± 0.131
9.394AlaVal: 9.394 ± 0.194
1.602AlaTrp: 1.602 ± 0.075
2.711AlaTyr: 2.711 ± 0.102
0.0AlaXaa: 0.0 ± 0.0
Cys
0.761CysAla: 0.761 ± 0.052
0.13CysCys: 0.13 ± 0.02
0.456CysAsp: 0.456 ± 0.045
0.442CysGlu: 0.442 ± 0.042
0.312CysPhe: 0.312 ± 0.033
1.112CysGly: 1.112 ± 0.075
0.284CysHis: 0.284 ± 0.029
0.316CysIle: 0.316 ± 0.041
0.126CysLys: 0.126 ± 0.021
1.171CysLeu: 1.171 ± 0.068
0.13CysMet: 0.13 ± 0.023
0.207CysAsn: 0.207 ± 0.029
0.684CysPro: 0.684 ± 0.062
0.54CysGln: 0.54 ± 0.047
0.943CysArg: 0.943 ± 0.054
0.445CysSer: 0.445 ± 0.042
0.424CysThr: 0.424 ± 0.042
0.515CysVal: 0.515 ± 0.046
0.105CysTrp: 0.105 ± 0.019
0.249CysTyr: 0.249 ± 0.029
0.0CysXaa: 0.0 ± 0.0
Asp
4.776AspAla: 4.776 ± 0.14
0.382AspCys: 0.382 ± 0.041
2.209AspAsp: 2.209 ± 0.103
3.447AspGlu: 3.447 ± 0.101
1.473AspPhe: 1.473 ± 0.072
4.457AspGly: 4.457 ± 0.188
0.824AspHis: 0.824 ± 0.056
2.255AspIle: 2.255 ± 0.09
0.698AspLys: 0.698 ± 0.054
5.035AspLeu: 5.035 ± 0.153
0.947AspMet: 0.947 ± 0.052
0.782AspAsn: 0.782 ± 0.072
3.443AspPro: 3.443 ± 0.137
1.199AspGln: 1.199 ± 0.071
3.408AspArg: 3.408 ± 0.112
1.834AspSer: 1.834 ± 0.083
1.753AspThr: 1.753 ± 0.077
3.98AspVal: 3.98 ± 0.101
0.715AspTrp: 0.715 ± 0.051
1.462AspTyr: 1.462 ± 0.071
0.0AspXaa: 0.0 ± 0.0
Glu
10.085GluAla: 10.085 ± 0.225
0.445GluCys: 0.445 ± 0.045
2.777GluAsp: 2.777 ± 0.106
8.128GluGlu: 8.128 ± 0.225
1.715GluPhe: 1.715 ± 0.076
7.017GluGly: 7.017 ± 0.182
1.445GluHis: 1.445 ± 0.068
2.693GluIle: 2.693 ± 0.113
1.574GluLys: 1.574 ± 0.078
8.153GluLeu: 8.153 ± 0.194
2.009GluMet: 2.009 ± 0.092
1.119GluAsn: 1.119 ± 0.066
3.089GluPro: 3.089 ± 0.109
2.279GluGln: 2.279 ± 0.098
8.167GluArg: 8.167 ± 0.193
2.272GluSer: 2.272 ± 0.092
3.096GluThr: 3.096 ± 0.108
6.708GluVal: 6.708 ± 0.142
0.95GluTrp: 0.95 ± 0.057
1.308GluTyr: 1.308 ± 0.074
0.0GluXaa: 0.0 ± 0.0
Phe
3.019PheAla: 3.019 ± 0.095
0.407PheCys: 0.407 ± 0.036
1.494PheAsp: 1.494 ± 0.076
1.364PheGlu: 1.364 ± 0.082
1.14PhePhe: 1.14 ± 0.074
2.647PheGly: 2.647 ± 0.093
0.754PheHis: 0.754 ± 0.047
1.189PheIle: 1.189 ± 0.066
0.554PheLys: 0.554 ± 0.045
3.882PheLeu: 3.882 ± 0.141
0.551PheMet: 0.551 ± 0.046
0.642PheAsn: 0.642 ± 0.053
1.897PhePro: 1.897 ± 0.079
1.347PheGln: 1.347 ± 0.064
2.528PheArg: 2.528 ± 0.093
1.466PheSer: 1.466 ± 0.079
1.504PheThr: 1.504 ± 0.074
2.507PheVal: 2.507 ± 0.101
0.533PheTrp: 0.533 ± 0.045
1.038PheTyr: 1.038 ± 0.064
0.0PheXaa: 0.0 ± 0.0
Gly
8.412GlyAla: 8.412 ± 0.207
0.978GlyCys: 0.978 ± 0.069
4.31GlyAsp: 4.31 ± 0.172
6.459GlyGlu: 6.459 ± 0.153
2.679GlyPhe: 2.679 ± 0.095
8.086GlyGly: 8.086 ± 0.241
2.044GlyHis: 2.044 ± 0.094
3.72GlyIle: 3.72 ± 0.122
1.953GlyLys: 1.953 ± 0.089
10.926GlyLeu: 10.926 ± 0.217
1.911GlyMet: 1.911 ± 0.081
1.511GlyAsn: 1.511 ± 0.075
5.582GlyPro: 5.582 ± 0.16
4.043GlyGln: 4.043 ± 0.145
8.787GlyArg: 8.787 ± 0.213
4.159GlySer: 4.159 ± 0.131
3.843GlyThr: 3.843 ± 0.111
7.269GlyVal: 7.269 ± 0.171
1.403GlyTrp: 1.403 ± 0.082
2.539GlyTyr: 2.539 ± 0.098
0.0GlyXaa: 0.0 ± 0.0
His
1.739HisAla: 1.739 ± 0.083
0.309HisCys: 0.309 ± 0.031
0.845HisAsp: 0.845 ± 0.051
1.231HisGlu: 1.231 ± 0.074
0.789HisPhe: 0.789 ± 0.057
1.708HisGly: 1.708 ± 0.089
0.631HisHis: 0.631 ± 0.05
0.884HisIle: 0.884 ± 0.048
0.396HisLys: 0.396 ± 0.04
2.819HisLeu: 2.819 ± 0.118
0.4HisMet: 0.4 ± 0.035
0.403HisAsn: 0.403 ± 0.039
1.697HisPro: 1.697 ± 0.083
0.771HisGln: 0.771 ± 0.054
1.809HisArg: 1.809 ± 0.09
0.789HisSer: 0.789 ± 0.048
0.929HisThr: 0.929 ± 0.049
1.476HisVal: 1.476 ± 0.068
0.316HisTrp: 0.316 ± 0.035
0.698HisTyr: 0.698 ± 0.052
0.0HisXaa: 0.0 ± 0.0
Ile
4.983IleAla: 4.983 ± 0.119
0.428IleCys: 0.428 ± 0.038
2.251IleAsp: 2.251 ± 0.089
2.721IleGlu: 2.721 ± 0.108
1.175IlePhe: 1.175 ± 0.064
3.535IleGly: 3.535 ± 0.122
0.663IleHis: 0.663 ± 0.058
1.823IleIle: 1.823 ± 0.101
0.95IleLys: 0.95 ± 0.056
3.177IleLeu: 3.177 ± 0.111
0.712IleMet: 0.712 ± 0.058
1.013IleAsn: 1.013 ± 0.069
2.1IlePro: 2.1 ± 0.082
1.101IleGln: 1.101 ± 0.062
2.718IleArg: 2.718 ± 0.097
1.883IleSer: 1.883 ± 0.091
1.837IleThr: 1.837 ± 0.096
4.071IleVal: 4.071 ± 0.132
0.449IleTrp: 0.449 ± 0.037
1.062IleTyr: 1.062 ± 0.059
0.0IleXaa: 0.0 ± 0.0
Lys
2.139LysAla: 2.139 ± 0.092
0.109LysCys: 0.109 ± 0.023
0.782LysAsp: 0.782 ± 0.056
1.746LysGlu: 1.746 ± 0.089
0.414LysPhe: 0.414 ± 0.038
1.806LysGly: 1.806 ± 0.075
0.351LysHis: 0.351 ± 0.035
0.778LysIle: 0.778 ± 0.057
0.484LysLys: 0.484 ± 0.043
1.813LysLeu: 1.813 ± 0.083
0.424LysMet: 0.424 ± 0.037
0.347LysAsn: 0.347 ± 0.038
0.814LysPro: 0.814 ± 0.046
0.477LysGln: 0.477 ± 0.039
1.659LysArg: 1.659 ± 0.081
0.771LysSer: 0.771 ± 0.048
0.922LysThr: 0.922 ± 0.049
1.827LysVal: 1.827 ± 0.081
0.217LysTrp: 0.217 ± 0.027
0.421LysTyr: 0.421 ± 0.039
0.0LysXaa: 0.0 ± 0.0
Leu
15.962LeuAla: 15.962 ± 0.318
1.02LeuCys: 1.02 ± 0.067
5.397LeuAsp: 5.397 ± 0.144
9.398LeuGlu: 9.398 ± 0.191
3.159LeuPhe: 3.159 ± 0.142
10.702LeuGly: 10.702 ± 0.228
2.325LeuHis: 2.325 ± 0.099
3.366LeuIle: 3.366 ± 0.121
2.125LeuLys: 2.125 ± 0.077
13.938LeuLeu: 13.938 ± 0.345
2.09LeuMet: 2.09 ± 0.077
1.631LeuAsn: 1.631 ± 0.068
7.311LeuPro: 7.311 ± 0.176
3.861LeuGln: 3.861 ± 0.144
10.944LeuArg: 10.944 ± 0.248
5.796LeuSer: 5.796 ± 0.177
5.186LeuThr: 5.186 ± 0.164
9.871LeuVal: 9.871 ± 0.246
1.662LeuTrp: 1.662 ± 0.094
2.644LeuTyr: 2.644 ± 0.111
0.0LeuXaa: 0.0 ± 0.0
Met
3.184MetAla: 3.184 ± 0.1
0.116MetCys: 0.116 ± 0.018
0.989MetAsp: 0.989 ± 0.065
1.581MetGlu: 1.581 ± 0.073
0.414MetPhe: 0.414 ± 0.037
1.992MetGly: 1.992 ± 0.093
0.245MetHis: 0.245 ± 0.028
0.508MetIle: 0.508 ± 0.042
0.501MetLys: 0.501 ± 0.043
1.851MetLeu: 1.851 ± 0.09
0.319MetMet: 0.319 ± 0.027
0.445MetAsn: 0.445 ± 0.044
1.196MetPro: 1.196 ± 0.067
0.526MetGln: 0.526 ± 0.047
1.76MetArg: 1.76 ± 0.084
0.926MetSer: 0.926 ± 0.055
1.168MetThr: 1.168 ± 0.062
1.732MetVal: 1.732 ± 0.086
0.228MetTrp: 0.228 ± 0.027
0.368MetTyr: 0.368 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
1.809AsnAla: 1.809 ± 0.086
0.252AsnCys: 0.252 ± 0.04
0.712AsnAsp: 0.712 ± 0.053
0.884AsnGlu: 0.884 ± 0.058
0.628AsnPhe: 0.628 ± 0.041
1.448AsnGly: 1.448 ± 0.074
0.337AsnHis: 0.337 ± 0.036
0.964AsnIle: 0.964 ± 0.057
0.421AsnLys: 0.421 ± 0.035
1.988AsnLeu: 1.988 ± 0.082
0.403AsnMet: 0.403 ± 0.039
0.407AsnAsn: 0.407 ± 0.038
1.311AsnPro: 1.311 ± 0.064
0.54AsnGln: 0.54 ± 0.043
1.311AsnArg: 1.311 ± 0.072
0.726AsnSer: 0.726 ± 0.054
0.733AsnThr: 0.733 ± 0.05
1.645AsnVal: 1.645 ± 0.087
0.242AsnTrp: 0.242 ± 0.03
0.526AsnTyr: 0.526 ± 0.04
0.0AsnXaa: 0.0 ± 0.0
Pro
6.108ProAla: 6.108 ± 0.166
0.456ProCys: 0.456 ± 0.043
3.286ProAsp: 3.286 ± 0.123
4.962ProGlu: 4.962 ± 0.126
2.037ProPhe: 2.037 ± 0.085
6.333ProGly: 6.333 ± 0.177
1.406ProHis: 1.406 ± 0.081
1.876ProIle: 1.876 ± 0.086
0.968ProLys: 0.968 ± 0.07
7.052ProLeu: 7.052 ± 0.168
1.126ProMet: 1.126 ± 0.074
1.077ProAsn: 1.077 ± 0.069
4.453ProPro: 4.453 ± 0.143
2.377ProGln: 2.377 ± 0.113
4.846ProArg: 4.846 ± 0.136
2.805ProSer: 2.805 ± 0.104
2.64ProThr: 2.64 ± 0.117
5.042ProVal: 5.042 ± 0.133
1.112ProTrp: 1.112 ± 0.062
1.581ProTyr: 1.581 ± 0.079
0.0ProXaa: 0.0 ± 0.0
Gln
4.516GlnAla: 4.516 ± 0.128
0.214GlnCys: 0.214 ± 0.028
1.455GlnAsp: 1.455 ± 0.075
3.608GlnGlu: 3.608 ± 0.121
0.912GlnPhe: 0.912 ± 0.056
3.801GlnGly: 3.801 ± 0.142
0.694GlnHis: 0.694 ± 0.05
1.087GlnIle: 1.087 ± 0.063
0.642GlnLys: 0.642 ± 0.054
3.156GlnLeu: 3.156 ± 0.112
0.729GlnMet: 0.729 ± 0.055
0.533GlnAsn: 0.533 ± 0.049
2.111GlnPro: 2.111 ± 0.082
1.49GlnGln: 1.49 ± 0.1
3.966GlnArg: 3.966 ± 0.146
1.196GlnSer: 1.196 ± 0.061
1.441GlnThr: 1.441 ± 0.09
3.619GlnVal: 3.619 ± 0.112
0.403GlnTrp: 0.403 ± 0.04
0.663GlnTyr: 0.663 ± 0.054
0.0GlnXaa: 0.0 ± 0.0
Arg
9.001ArgAla: 9.001 ± 0.19
0.831ArgCys: 0.831 ± 0.055
3.664ArgAsp: 3.664 ± 0.114
7.96ArgGlu: 7.96 ± 0.191
3.1ArgPhe: 3.1 ± 0.111
7.202ArgGly: 7.202 ± 0.176
2.009ArgHis: 2.009 ± 0.075
3.507ArgIle: 3.507 ± 0.112
1.347ArgLys: 1.347 ± 0.068
12.613ArgLeu: 12.613 ± 0.265
1.732ArgMet: 1.732 ± 0.083
1.476ArgAsn: 1.476 ± 0.074
6.396ArgPro: 6.396 ± 0.168
4.015ArgGln: 4.015 ± 0.125
9.888ArgArg: 9.888 ± 0.214
3.878ArgSer: 3.878 ± 0.117
3.159ArgThr: 3.159 ± 0.101
6.164ArgVal: 6.164 ± 0.15
1.694ArgTrp: 1.694 ± 0.093
2.739ArgTyr: 2.739 ± 0.118
0.0ArgXaa: 0.0 ± 0.0
Ser
4.089SerAla: 4.089 ± 0.134
0.438SerCys: 0.438 ± 0.046
1.932SerAsp: 1.932 ± 0.099
2.199SerGlu: 2.199 ± 0.087
1.778SerPhe: 1.778 ± 0.091
4.099SerGly: 4.099 ± 0.146
0.87SerHis: 0.87 ± 0.049
1.715SerIle: 1.715 ± 0.089
0.67SerLys: 0.67 ± 0.058
6.396SerLeu: 6.396 ± 0.165
0.929SerMet: 0.929 ± 0.052
0.701SerAsn: 0.701 ± 0.049
3.626SerPro: 3.626 ± 0.13
1.571SerGln: 1.571 ± 0.076
3.57SerArg: 3.57 ± 0.098
2.132SerSer: 2.132 ± 0.1
1.883SerThr: 1.883 ± 0.094
3.342SerVal: 3.342 ± 0.115
0.698SerTrp: 0.698 ± 0.051
1.35SerTyr: 1.35 ± 0.073
0.0SerXaa: 0.0 ± 0.0
Thr
4.895ThrAla: 4.895 ± 0.129
0.438ThrCys: 0.438 ± 0.041
2.006ThrAsp: 2.006 ± 0.101
2.542ThrGlu: 2.542 ± 0.09
1.49ThrPhe: 1.49 ± 0.071
4.411ThrGly: 4.411 ± 0.145
0.747ThrHis: 0.747 ± 0.055
1.904ThrIle: 1.904 ± 0.092
0.712ThrLys: 0.712 ± 0.049
4.727ThrLeu: 4.727 ± 0.136
0.852ThrMet: 0.852 ± 0.046
0.785ThrAsn: 0.785 ± 0.063
3.191ThrPro: 3.191 ± 0.109
0.996ThrGln: 0.996 ± 0.057
2.809ThrArg: 2.809 ± 0.117
2.097ThrSer: 2.097 ± 0.084
2.076ThrThr: 2.076 ± 0.089
4.699ThrVal: 4.699 ± 0.135
0.607ThrTrp: 0.607 ± 0.056
1.189ThrTyr: 1.189 ± 0.067
0.0ThrXaa: 0.0 ± 0.0
Val
10.278ValAla: 10.278 ± 0.197
0.803ValCys: 0.803 ± 0.054
3.738ValAsp: 3.738 ± 0.112
6.112ValGlu: 6.112 ± 0.174
2.5ValPhe: 2.5 ± 0.102
6.613ValGly: 6.613 ± 0.193
1.89ValHis: 1.89 ± 0.08
3.636ValIle: 3.636 ± 0.104
1.581ValLys: 1.581 ± 0.078
9.471ValLeu: 9.471 ± 0.203
1.624ValMet: 1.624 ± 0.078
1.83ValAsn: 1.83 ± 0.083
5.106ValPro: 5.106 ± 0.145
2.949ValGln: 2.949 ± 0.105
7.507ValArg: 7.507 ± 0.181
3.805ValSer: 3.805 ± 0.127
4.208ValThr: 4.208 ± 0.136
7.76ValVal: 7.76 ± 0.175
1.091ValTrp: 1.091 ± 0.07
2.062ValTyr: 2.062 ± 0.086
0.0ValXaa: 0.0 ± 0.0
Trp
1.564TrpAla: 1.564 ± 0.076
0.182TrpCys: 0.182 ± 0.025
0.722TrpAsp: 0.722 ± 0.054
1.336TrpGlu: 1.336 ± 0.072
0.424TrpPhe: 0.424 ± 0.043
1.28TrpGly: 1.28 ± 0.069
0.263TrpHis: 0.263 ± 0.033
0.354TrpIle: 0.354 ± 0.033
0.175TrpLys: 0.175 ± 0.024
1.834TrpLeu: 1.834 ± 0.084
0.259TrpMet: 0.259 ± 0.036
0.266TrpAsn: 0.266 ± 0.03
0.891TrpPro: 0.891 ± 0.055
0.831TrpGln: 0.831 ± 0.059
1.574TrpArg: 1.574 ± 0.072
0.551TrpSer: 0.551 ± 0.044
0.649TrpThr: 0.649 ± 0.053
0.968TrpVal: 0.968 ± 0.063
0.347TrpTrp: 0.347 ± 0.033
0.351TrpTyr: 0.351 ± 0.038
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.325TyrAla: 2.325 ± 0.09
0.323TyrCys: 0.323 ± 0.033
1.438TyrAsp: 1.438 ± 0.08
1.574TyrGlu: 1.574 ± 0.066
0.912TyrPhe: 0.912 ± 0.07
2.3TyrGly: 2.3 ± 0.099
0.624TyrHis: 0.624 ± 0.048
1.069TyrIle: 1.069 ± 0.062
0.407TyrLys: 0.407 ± 0.038
3.135TyrLeu: 3.135 ± 0.111
0.445TyrMet: 0.445 ± 0.039
0.519TyrAsn: 0.519 ± 0.042
1.322TyrPro: 1.322 ± 0.072
0.992TyrGln: 0.992 ± 0.062
2.819TyrArg: 2.819 ± 0.097
1.224TyrSer: 1.224 ± 0.066
1.108TyrThr: 1.108 ± 0.061
1.974TyrVal: 1.974 ± 0.09
0.449TyrTrp: 0.449 ± 0.041
0.824TyrTyr: 0.824 ± 0.05
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 982 proteins (285183 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski