Piptocephalis cylindrospora

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Zoopagomycota; Zoopagomycotina; Zoopagomycetes; Zoopagales; Piptocephalidaceae; Piptocephalis

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4271 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P9Y3C8|A0A4P9Y3C8_9FUNG BZIP domain-containing protein OS=Piptocephalis cylindrospora OX=1907219 GN=BJ684DRAFT_20301 PE=4 SV=1
MM1 pKa = 7.51KK2 pKa = 10.36SALILAAAAAAITPASATLFSSTLACIVNSQRR34 pKa = 11.84QKK36 pKa = 11.27YY37 pKa = 10.15DD38 pKa = 3.24LTPVKK43 pKa = 8.65TTTADD48 pKa = 3.45FNEE51 pKa = 4.73LQTEE55 pKa = 4.56AHH57 pKa = 5.89QMSSTKK63 pKa = 10.33KK64 pKa = 9.81VIPGSFGNGWRR75 pKa = 11.84KK76 pKa = 9.63IPVQFTCPYY85 pKa = 7.79AHH87 pKa = 7.39EE88 pKa = 4.26YY89 pKa = 11.08DD90 pKa = 3.68GTNEE94 pKa = 3.77VSVVNVINNVTHH106 pKa = 7.36SMDD109 pKa = 3.03ILNAKK114 pKa = 9.91NITAVSCSAHH124 pKa = 6.49KK125 pKa = 10.57EE126 pKa = 3.53VDD128 pKa = 3.59QVDD131 pKa = 3.69KK132 pKa = 10.67KK133 pKa = 9.42TGKK136 pKa = 8.41KK137 pKa = 7.9TRR139 pKa = 11.84NSHH142 pKa = 4.85CWGMVKK148 pKa = 9.54IYY150 pKa = 9.57YY151 pKa = 8.89TDD153 pKa = 2.97SGNFVPAANNCTSTPPPTDD172 pKa = 2.82GGYY175 pKa = 8.38TPPGDD180 pKa = 3.9GNQDD184 pKa = 2.89TCYY187 pKa = 10.44EE188 pKa = 4.13GSTDD192 pKa = 4.28PKK194 pKa = 9.28CTSPGGDD201 pKa = 3.09TTYY204 pKa = 10.48TPPGGDD210 pKa = 2.99TTYY213 pKa = 10.58TPPGDD218 pKa = 3.79DD219 pKa = 3.06TTYY222 pKa = 10.84TPPGDD227 pKa = 3.9NNQDD231 pKa = 2.95TCYY234 pKa = 10.51EE235 pKa = 3.99GSTDD239 pKa = 4.43SKK241 pKa = 10.01CTSPGGDD248 pKa = 3.17TTYY251 pKa = 10.71TPPDD255 pKa = 3.51NKK257 pKa = 10.57DD258 pKa = 4.04DD259 pKa = 4.15EE260 pKa = 4.89SCDD263 pKa = 4.34DD264 pKa = 3.68STTPGNGGTSPGGDD278 pKa = 3.04TTYY281 pKa = 10.73TPPGDD286 pKa = 3.58NTTYY290 pKa = 10.36TPPGKK295 pKa = 10.11DD296 pKa = 3.78DD297 pKa = 5.29DD298 pKa = 4.68EE299 pKa = 6.22KK300 pKa = 11.47CDD302 pKa = 3.82IYY304 pKa = 10.93STSDD308 pKa = 2.61KK309 pKa = 10.8CNPGNEE315 pKa = 4.33DD316 pKa = 4.04EE317 pKa = 6.05GDD319 pKa = 3.68DD320 pKa = 4.05TYY322 pKa = 11.59TPPGKK327 pKa = 10.14DD328 pKa = 3.78DD329 pKa = 5.29DD330 pKa = 4.68EE331 pKa = 6.22KK332 pKa = 11.47CDD334 pKa = 3.82IYY336 pKa = 10.93STSDD340 pKa = 2.71KK341 pKa = 10.98CNPGNDD347 pKa = 4.19KK348 pKa = 11.39DD349 pKa = 3.92DD350 pKa = 3.69TTYY353 pKa = 11.71SNDD356 pKa = 3.67EE357 pKa = 4.5DD358 pKa = 3.84DD359 pKa = 3.71TTYY362 pKa = 11.66SNDD365 pKa = 3.67EE366 pKa = 4.5DD367 pKa = 3.84DD368 pKa = 3.71TTYY371 pKa = 11.66SNDD374 pKa = 3.54EE375 pKa = 4.77DD376 pKa = 5.9DD377 pKa = 5.82SGDD380 pKa = 4.14DD381 pKa = 4.41SDD383 pKa = 6.28DD384 pKa = 4.61GSDD387 pKa = 3.97DD388 pKa = 4.13DD389 pKa = 6.24QYY391 pKa = 12.14NQDD394 pKa = 3.17NGYY397 pKa = 10.59

Molecular weight: 42.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P9XZC9|A0A4P9XZC9_9FUNG Uncharacterized protein OS=Piptocephalis cylindrospora OX=1907219 GN=BJ684DRAFT_21685 PE=4 SV=1
NN1 pKa = 7.39LEE3 pKa = 4.13LYY5 pKa = 10.13QRR7 pKa = 11.84LNFLHH12 pKa = 6.05QASTLLTLAGPHH24 pKa = 6.26FAPLSRR30 pKa = 11.84SLGRR34 pKa = 11.84DD35 pKa = 2.81MDD37 pKa = 4.43SVRR40 pKa = 11.84KK41 pKa = 9.88RR42 pKa = 11.84STLRR46 pKa = 11.84ADD48 pKa = 3.6PSVKK52 pKa = 9.9RR53 pKa = 11.84QYY55 pKa = 10.98CRR57 pKa = 11.84ACHH60 pKa = 4.82THH62 pKa = 7.3LIEE65 pKa = 4.96GFTLRR70 pKa = 11.84TRR72 pKa = 11.84LTRR75 pKa = 11.84KK76 pKa = 9.18GGKK79 pKa = 9.95AIIRR83 pKa = 11.84TCMYY87 pKa = 10.07CEE89 pKa = 3.83KK90 pKa = 10.37EE91 pKa = 4.5RR92 pKa = 11.84RR93 pKa = 11.84LLCNPQHH100 pKa = 6.97EE101 pKa = 4.7VFNWKK106 pKa = 9.81

Molecular weight: 12.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4271

0

4271

1596308

50

3304

373.8

41.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.833 ± 0.038

1.205 ± 0.014

5.39 ± 0.029

6.776 ± 0.049

3.206 ± 0.027

7.599 ± 0.046

2.666 ± 0.019

4.665 ± 0.028

4.502 ± 0.036

9.832 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.443 ± 0.016

2.696 ± 0.019

6.133 ± 0.048

3.789 ± 0.022

6.718 ± 0.038

8.7 ± 0.058

5.707 ± 0.029

6.044 ± 0.028

1.449 ± 0.017

2.647 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski