Amino acid dipepetide frequency for Rhizobium sp. NFR07

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.305AlaAla: 15.305 ± 0.127
0.912AlaCys: 0.912 ± 0.025
6.631AlaAsp: 6.631 ± 0.072
7.309AlaGlu: 7.309 ± 0.085
4.541AlaPhe: 4.541 ± 0.051
9.987AlaGly: 9.987 ± 0.082
2.056AlaHis: 2.056 ± 0.033
7.059AlaIle: 7.059 ± 0.067
4.366AlaLys: 4.366 ± 0.055
12.126AlaLeu: 12.126 ± 0.113
3.587AlaMet: 3.587 ± 0.048
3.02AlaAsn: 3.02 ± 0.059
4.817AlaPro: 4.817 ± 0.068
3.516AlaGln: 3.516 ± 0.051
7.352AlaArg: 7.352 ± 0.079
6.898AlaSer: 6.898 ± 0.068
5.903AlaThr: 5.903 ± 0.078
8.423AlaVal: 8.423 ± 0.071
1.315AlaTrp: 1.315 ± 0.03
2.536AlaTyr: 2.536 ± 0.035
0.0AlaXaa: 0.0 ± 0.0
Cys
0.805CysAla: 0.805 ± 0.023
0.102CysCys: 0.102 ± 0.007
0.484CysAsp: 0.484 ± 0.015
0.424CysGlu: 0.424 ± 0.016
0.301CysPhe: 0.301 ± 0.014
0.852CysGly: 0.852 ± 0.024
0.206CysHis: 0.206 ± 0.012
0.391CysIle: 0.391 ± 0.015
0.191CysLys: 0.191 ± 0.01
0.792CysLeu: 0.792 ± 0.023
0.174CysMet: 0.174 ± 0.009
0.194CysAsn: 0.194 ± 0.01
0.366CysPro: 0.366 ± 0.014
0.226CysGln: 0.226 ± 0.011
0.534CysArg: 0.534 ± 0.018
0.443CysSer: 0.443 ± 0.016
0.352CysThr: 0.352 ± 0.014
0.575CysVal: 0.575 ± 0.018
0.1CysTrp: 0.1 ± 0.006
0.188CysTyr: 0.188 ± 0.01
0.0CysXaa: 0.0 ± 0.0
Asp
6.639AspAla: 6.639 ± 0.07
0.473AspCys: 0.473 ± 0.016
3.266AspAsp: 3.266 ± 0.053
3.612AspGlu: 3.612 ± 0.047
2.371AspPhe: 2.371 ± 0.036
5.236AspGly: 5.236 ± 0.098
1.261AspHis: 1.261 ± 0.026
3.481AspIle: 3.481 ± 0.04
1.924AspLys: 1.924 ± 0.036
5.883AspLeu: 5.883 ± 0.062
1.506AspMet: 1.506 ± 0.03
1.508AspAsn: 1.508 ± 0.028
3.252AspPro: 3.252 ± 0.048
1.77AspGln: 1.77 ± 0.032
4.265AspArg: 4.265 ± 0.06
2.069AspSer: 2.069 ± 0.046
2.672AspThr: 2.672 ± 0.072
4.188AspVal: 4.188 ± 0.049
0.939AspTrp: 0.939 ± 0.026
1.525AspTyr: 1.525 ± 0.025
0.0AspXaa: 0.0 ± 0.0
Glu
7.224GluAla: 7.224 ± 0.088
0.322GluCys: 0.322 ± 0.014
3.043GluAsp: 3.043 ± 0.044
3.549GluGlu: 3.549 ± 0.063
1.888GluPhe: 1.888 ± 0.031
4.275GluGly: 4.275 ± 0.056
1.176GluHis: 1.176 ± 0.027
3.86GluIle: 3.86 ± 0.051
2.814GluLys: 2.814 ± 0.047
5.437GluLeu: 5.437 ± 0.069
1.621GluMet: 1.621 ± 0.028
1.906GluAsn: 1.906 ± 0.034
2.714GluPro: 2.714 ± 0.042
2.214GluGln: 2.214 ± 0.039
4.572GluArg: 4.572 ± 0.06
2.293GluSer: 2.293 ± 0.034
3.682GluThr: 3.682 ± 0.043
3.775GluVal: 3.775 ± 0.045
0.732GluTrp: 0.732 ± 0.023
1.041GluTyr: 1.041 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
4.49PheAla: 4.49 ± 0.054
0.391PheCys: 0.391 ± 0.014
2.795PheAsp: 2.795 ± 0.04
2.248PheGlu: 2.248 ± 0.036
1.608PhePhe: 1.608 ± 0.033
3.783PheGly: 3.783 ± 0.055
0.77PheHis: 0.77 ± 0.02
1.985PheIle: 1.985 ± 0.034
1.137PheLys: 1.137 ± 0.025
3.598PheLeu: 3.598 ± 0.051
0.894PheMet: 0.894 ± 0.025
1.153PheAsn: 1.153 ± 0.036
1.604PhePro: 1.604 ± 0.028
1.106PheGln: 1.106 ± 0.022
2.314PheArg: 2.314 ± 0.037
2.634PheSer: 2.634 ± 0.037
2.019PheThr: 2.019 ± 0.055
2.94PheVal: 2.94 ± 0.042
0.583PheTrp: 0.583 ± 0.021
0.969PheTyr: 0.969 ± 0.021
0.0PheXaa: 0.0 ± 0.0
Gly
8.348GlyAla: 8.348 ± 0.072
0.807GlyCys: 0.807 ± 0.02
4.342GlyAsp: 4.342 ± 0.06
4.858GlyGlu: 4.858 ± 0.052
3.666GlyPhe: 3.666 ± 0.043
6.999GlyGly: 6.999 ± 0.075
1.84GlyHis: 1.84 ± 0.038
5.06GlyIle: 5.06 ± 0.059
3.604GlyLys: 3.604 ± 0.046
8.471GlyLeu: 8.471 ± 0.073
2.425GlyMet: 2.425 ± 0.038
2.398GlyAsn: 2.398 ± 0.048
3.189GlyPro: 3.189 ± 0.044
2.804GlyGln: 2.804 ± 0.047
5.789GlyArg: 5.789 ± 0.059
4.938GlySer: 4.938 ± 0.071
4.605GlyThr: 4.605 ± 0.134
5.698GlyVal: 5.698 ± 0.059
1.272GlyTrp: 1.272 ± 0.027
2.364GlyTyr: 2.364 ± 0.036
0.0GlyXaa: 0.0 ± 0.0
His
2.115HisAla: 2.115 ± 0.036
0.214HisCys: 0.214 ± 0.011
1.254HisAsp: 1.254 ± 0.03
1.097HisGlu: 1.097 ± 0.025
0.862HisPhe: 0.862 ± 0.02
1.877HisGly: 1.877 ± 0.037
0.588HisHis: 0.588 ± 0.021
0.969HisIle: 0.969 ± 0.023
0.525HisLys: 0.525 ± 0.015
1.999HisLeu: 1.999 ± 0.04
0.539HisMet: 0.539 ± 0.018
0.46HisAsn: 0.46 ± 0.014
1.226HisPro: 1.226 ± 0.025
0.597HisGln: 0.597 ± 0.018
1.339HisArg: 1.339 ± 0.027
1.019HisSer: 1.019 ± 0.025
0.773HisThr: 0.773 ± 0.021
1.537HisVal: 1.537 ± 0.025
0.304HisTrp: 0.304 ± 0.011
0.551HisTyr: 0.551 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
7.853IleAla: 7.853 ± 0.06
0.56IleCys: 0.56 ± 0.016
3.863IleAsp: 3.863 ± 0.046
3.779IleGlu: 3.779 ± 0.046
2.088IlePhe: 2.088 ± 0.035
5.512IleGly: 5.512 ± 0.062
0.984IleHis: 0.984 ± 0.024
2.78IleIle: 2.78 ± 0.046
1.666IleLys: 1.666 ± 0.034
4.923IleLeu: 4.923 ± 0.061
1.201IleMet: 1.201 ± 0.027
1.613IleAsn: 1.613 ± 0.038
2.535IlePro: 2.535 ± 0.036
1.341IleGln: 1.341 ± 0.027
3.575IleArg: 3.575 ± 0.047
3.603IleSer: 3.603 ± 0.05
2.994IleThr: 2.994 ± 0.058
4.682IleVal: 4.682 ± 0.056
0.641IleTrp: 0.641 ± 0.017
1.303IleTyr: 1.303 ± 0.026
0.0IleXaa: 0.0 ± 0.0
Lys
4.702LysAla: 4.702 ± 0.062
0.146LysCys: 0.146 ± 0.01
2.023LysAsp: 2.023 ± 0.035
1.859LysGlu: 1.859 ± 0.036
1.039LysPhe: 1.039 ± 0.024
2.848LysGly: 2.848 ± 0.042
0.629LysHis: 0.629 ± 0.019
2.092LysIle: 2.092 ± 0.036
1.516LysLys: 1.516 ± 0.034
3.68LysLeu: 3.68 ± 0.052
0.908LysMet: 0.908 ± 0.023
1.057LysAsn: 1.057 ± 0.022
2.283LysPro: 2.283 ± 0.038
1.144LysGln: 1.144 ± 0.026
2.446LysArg: 2.446 ± 0.038
2.222LysSer: 2.222 ± 0.041
2.244LysThr: 2.244 ± 0.035
2.668LysVal: 2.668 ± 0.041
0.398LysTrp: 0.398 ± 0.014
0.669LysTyr: 0.669 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
12.416LeuAla: 12.416 ± 0.105
0.83LeuCys: 0.83 ± 0.02
5.808LeuAsp: 5.808 ± 0.059
5.206LeuGlu: 5.206 ± 0.072
3.682LeuPhe: 3.682 ± 0.053
7.814LeuGly: 7.814 ± 0.075
1.769LeuHis: 1.769 ± 0.034
5.306LeuIle: 5.306 ± 0.063
4.057LeuLys: 4.057 ± 0.045
9.097LeuLeu: 9.097 ± 0.104
2.444LeuMet: 2.444 ± 0.037
2.694LeuAsn: 2.694 ± 0.037
5.197LeuPro: 5.197 ± 0.057
2.851LeuGln: 2.851 ± 0.04
6.049LeuArg: 6.049 ± 0.068
7.121LeuSer: 7.121 ± 0.069
5.633LeuThr: 5.633 ± 0.068
7.266LeuVal: 7.266 ± 0.075
1.062LeuTrp: 1.062 ± 0.025
2.036LeuTyr: 2.036 ± 0.034
0.0LeuXaa: 0.0 ± 0.0
Met
3.185MetAla: 3.185 ± 0.04
0.13MetCys: 0.13 ± 0.008
1.258MetAsp: 1.258 ± 0.027
1.226MetGlu: 1.226 ± 0.024
0.759MetPhe: 0.759 ± 0.022
1.785MetGly: 1.785 ± 0.034
0.433MetHis: 0.433 ± 0.017
1.61MetIle: 1.61 ± 0.029
1.147MetLys: 1.147 ± 0.027
2.706MetLeu: 2.706 ± 0.043
0.759MetMet: 0.759 ± 0.019
0.861MetAsn: 0.861 ± 0.021
1.609MetPro: 1.609 ± 0.029
0.905MetGln: 0.905 ± 0.022
1.829MetArg: 1.829 ± 0.035
1.88MetSer: 1.88 ± 0.032
2.093MetThr: 2.093 ± 0.033
1.806MetVal: 1.806 ± 0.031
0.21MetTrp: 0.21 ± 0.011
0.328MetTyr: 0.328 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.387AsnAla: 3.387 ± 0.066
0.241AsnCys: 0.241 ± 0.011
1.621AsnAsp: 1.621 ± 0.061
1.471AsnGlu: 1.471 ± 0.028
1.122AsnPhe: 1.122 ± 0.026
2.689AsnGly: 2.689 ± 0.094
0.551AsnHis: 0.551 ± 0.019
1.552AsnIle: 1.552 ± 0.026
0.797AsnLys: 0.797 ± 0.021
2.731AsnLeu: 2.731 ± 0.043
0.7AsnMet: 0.7 ± 0.019
0.804AsnAsn: 0.804 ± 0.02
1.829AsnPro: 1.829 ± 0.034
0.798AsnGln: 0.798 ± 0.019
1.885AsnArg: 1.885 ± 0.032
1.467AsnSer: 1.467 ± 0.031
1.387AsnThr: 1.387 ± 0.035
2.084AsnVal: 2.084 ± 0.035
0.445AsnTrp: 0.445 ± 0.015
0.686AsnTyr: 0.686 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
5.67ProAla: 5.67 ± 0.07
0.251ProCys: 0.251 ± 0.013
3.437ProAsp: 3.437 ± 0.048
3.45ProGlu: 3.45 ± 0.046
2.014ProPhe: 2.014 ± 0.032
4.007ProGly: 4.007 ± 0.046
1.055ProHis: 1.055 ± 0.023
2.502ProIle: 2.502 ± 0.038
1.841ProLys: 1.841 ± 0.032
4.438ProLeu: 4.438 ± 0.05
1.21ProMet: 1.21 ± 0.023
1.403ProAsn: 1.403 ± 0.03
2.14ProPro: 2.14 ± 0.045
1.627ProGln: 1.627 ± 0.031
2.528ProArg: 2.528 ± 0.036
2.927ProSer: 2.927 ± 0.043
2.536ProThr: 2.536 ± 0.039
4.029ProVal: 4.029 ± 0.071
0.615ProTrp: 0.615 ± 0.018
1.202ProTyr: 1.202 ± 0.026
0.0ProXaa: 0.0 ± 0.0
Gln
3.942GlnAla: 3.942 ± 0.046
0.168GlnCys: 0.168 ± 0.01
1.477GlnAsp: 1.477 ± 0.029
1.6GlnGlu: 1.6 ± 0.032
1.124GlnPhe: 1.124 ± 0.025
2.269GlnGly: 2.269 ± 0.031
0.615GlnHis: 0.615 ± 0.018
1.963GlnIle: 1.963 ± 0.044
1.209GlnLys: 1.209 ± 0.023
2.914GlnLeu: 2.914 ± 0.045
0.957GlnMet: 0.957 ± 0.024
0.927GlnAsn: 0.927 ± 0.02
1.754GlnPro: 1.754 ± 0.033
1.396GlnGln: 1.396 ± 0.033
2.188GlnArg: 2.188 ± 0.035
1.965GlnSer: 1.965 ± 0.034
1.777GlnThr: 1.777 ± 0.033
2.202GlnVal: 2.202 ± 0.034
0.393GlnTrp: 0.393 ± 0.015
0.643GlnTyr: 0.643 ± 0.02
0.0GlnXaa: 0.0 ± 0.0
Arg
6.443ArgAla: 6.443 ± 0.064
0.435ArgCys: 0.435 ± 0.017
3.9ArgAsp: 3.9 ± 0.049
4.012ArgGlu: 4.012 ± 0.05
2.749ArgPhe: 2.749 ± 0.042
4.384ArgGly: 4.384 ± 0.049
1.629ArgHis: 1.629 ± 0.031
4.048ArgIle: 4.048 ± 0.048
2.433ArgLys: 2.433 ± 0.036
7.1ArgLeu: 7.1 ± 0.072
1.946ArgMet: 1.946 ± 0.03
2.021ArgAsn: 2.021 ± 0.033
3.134ArgPro: 3.134 ± 0.048
2.652ArgGln: 2.652 ± 0.034
5.046ArgArg: 5.046 ± 0.064
3.958ArgSer: 3.958 ± 0.057
3.18ArgThr: 3.18 ± 0.048
4.174ArgVal: 4.174 ± 0.051
0.92ArgTrp: 0.92 ± 0.024
1.786ArgTyr: 1.786 ± 0.032
0.0ArgXaa: 0.0 ± 0.0
Ser
6.607SerAla: 6.607 ± 0.068
0.406SerCys: 0.406 ± 0.015
3.329SerAsp: 3.329 ± 0.07
3.172SerGlu: 3.172 ± 0.043
2.591SerPhe: 2.591 ± 0.044
5.747SerGly: 5.747 ± 0.054
1.186SerHis: 1.186 ± 0.027
3.339SerIle: 3.339 ± 0.042
1.923SerLys: 1.923 ± 0.033
5.949SerLeu: 5.949 ± 0.071
1.548SerMet: 1.548 ± 0.029
1.593SerAsn: 1.593 ± 0.029
2.882SerPro: 2.882 ± 0.042
1.813SerGln: 1.813 ± 0.032
3.856SerArg: 3.856 ± 0.053
3.629SerSer: 3.629 ± 0.05
3.065SerThr: 3.065 ± 0.045
4.37SerVal: 4.37 ± 0.053
0.795SerTrp: 0.795 ± 0.021
1.459SerTyr: 1.459 ± 0.034
0.0SerXaa: 0.0 ± 0.0
Thr
6.212ThrAla: 6.212 ± 0.084
0.392ThrCys: 0.392 ± 0.017
3.062ThrAsp: 3.062 ± 0.065
2.851ThrGlu: 2.851 ± 0.043
2.172ThrPhe: 2.172 ± 0.041
4.985ThrGly: 4.985 ± 0.065
0.99ThrHis: 0.99 ± 0.024
3.372ThrIle: 3.372 ± 0.077
1.687ThrLys: 1.687 ± 0.029
5.525ThrLeu: 5.525 ± 0.066
1.329ThrMet: 1.329 ± 0.027
1.45ThrAsn: 1.45 ± 0.04
3.062ThrPro: 3.062 ± 0.049
1.439ThrGln: 1.439 ± 0.03
3.14ThrArg: 3.14 ± 0.043
3.188ThrSer: 3.188 ± 0.049
2.912ThrThr: 2.912 ± 0.061
4.612ThrVal: 4.612 ± 0.135
0.69ThrTrp: 0.69 ± 0.02
1.338ThrTyr: 1.338 ± 0.082
0.0ThrXaa: 0.0 ± 0.0
Val
8.773ValAla: 8.773 ± 0.074
0.562ValCys: 0.562 ± 0.017
4.103ValAsp: 4.103 ± 0.053
4.575ValGlu: 4.575 ± 0.049
2.885ValPhe: 2.885 ± 0.044
5.46ValGly: 5.46 ± 0.061
1.329ValHis: 1.329 ± 0.027
4.399ValIle: 4.399 ± 0.054
2.557ValLys: 2.557 ± 0.037
7.086ValLeu: 7.086 ± 0.071
1.928ValMet: 1.928 ± 0.032
2.087ValAsn: 2.087 ± 0.062
3.453ValPro: 3.453 ± 0.038
1.968ValGln: 1.968 ± 0.036
4.45ValArg: 4.45 ± 0.053
4.826ValSer: 4.826 ± 0.055
4.686ValThr: 4.686 ± 0.117
5.808ValVal: 5.808 ± 0.071
0.819ValTrp: 0.819 ± 0.021
1.509ValTyr: 1.509 ± 0.029
0.0ValXaa: 0.0 ± 0.0
Trp
1.115TrpAla: 1.115 ± 0.024
0.124TrpCys: 0.124 ± 0.008
0.648TrpAsp: 0.648 ± 0.021
0.555TrpGlu: 0.555 ± 0.018
0.562TrpPhe: 0.562 ± 0.015
0.833TrpGly: 0.833 ± 0.022
0.297TrpHis: 0.297 ± 0.011
0.72TrpIle: 0.72 ± 0.02
0.521TrpLys: 0.521 ± 0.015
1.556TrpLeu: 1.556 ± 0.029
0.368TrpMet: 0.368 ± 0.015
0.473TrpAsn: 0.473 ± 0.017
0.673TrpPro: 0.673 ± 0.019
0.554TrpGln: 0.554 ± 0.016
1.017TrpArg: 1.017 ± 0.027
0.834TrpSer: 0.834 ± 0.024
0.741TrpThr: 0.741 ± 0.02
0.725TrpVal: 0.725 ± 0.022
0.206TrpTrp: 0.206 ± 0.011
0.285TrpTyr: 0.285 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.428TyrAla: 2.428 ± 0.044
0.236TyrCys: 0.236 ± 0.011
1.494TyrAsp: 1.494 ± 0.029
1.285TyrGlu: 1.285 ± 0.026
0.993TyrPhe: 0.993 ± 0.026
2.135TyrGly: 2.135 ± 0.038
0.482TyrHis: 0.482 ± 0.016
1.007TyrIle: 1.007 ± 0.023
0.662TyrLys: 0.662 ± 0.019
2.326TyrLeu: 2.326 ± 0.041
0.475TyrMet: 0.475 ± 0.015
0.628TyrAsn: 0.628 ± 0.021
1.16TyrPro: 1.16 ± 0.025
0.762TyrGln: 0.762 ± 0.02
1.776TyrArg: 1.776 ± 0.032
1.347TyrSer: 1.347 ± 0.029
1.153TyrThr: 1.153 ± 0.076
1.7TyrVal: 1.7 ± 0.03
0.368TyrTrp: 0.368 ± 0.012
0.673TyrTyr: 0.673 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 6556 proteins (1986648 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski