Amino acid dipepetide frequency for Spirochaeta thermophila (strain ATCC 700085 / DSM 6578 / Z-1203)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.36AlaAla: 7.36 ± 0.138	0.818AlaCys: 0.818 ± 0.035	3.387AlaAsp: 3.387 ± 0.065	5.657AlaGlu: 5.657 ± 0.092	3.772AlaPhe: 3.772 ± 0.072	7.14AlaGly: 7.14 ± 0.113	1.904AlaHis: 1.904 ± 0.05	3.698AlaIle: 3.698 ± 0.086	2.408AlaLys: 2.408 ± 0.058	10.414AlaLeu: 10.414 ± 0.139	1.701AlaMet: 1.701 ± 0.048	1.369AlaAsn: 1.369 ± 0.044	3.443AlaPro: 3.443 ± 0.075	2.367AlaGln: 2.367 ± 0.058	7.027AlaArg: 7.027 ± 0.114	4.77AlaSer: 4.77 ± 0.087	3.307AlaThr: 3.307 ± 0.066	6.954AlaVal: 6.954 ± 0.118	1.092AlaTrp: 1.092 ± 0.039	2.832AlaTyr: 2.832 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	0.793CysAla: 0.793 ± 0.033	0.084CysCys: 0.084 ± 0.011	0.388CysAsp: 0.388 ± 0.024	0.576CysGlu: 0.576 ± 0.029	0.322CysPhe: 0.322 ± 0.02	0.946CysGly: 0.946 ± 0.046	0.184CysHis: 0.184 ± 0.015	0.431CysIle: 0.431 ± 0.022	0.19CysLys: 0.19 ± 0.016	0.667CysLeu: 0.667 ± 0.026	0.15CysMet: 0.15 ± 0.014	0.179CysAsn: 0.179 ± 0.013	0.525CysPro: 0.525 ± 0.027	0.114CysGln: 0.114 ± 0.012	0.629CysArg: 0.629 ± 0.029	0.454CysSer: 0.454 ± 0.025	0.505CysThr: 0.505 ± 0.029	0.69CysVal: 0.69 ± 0.027	0.099CysTrp: 0.099 ± 0.012	0.287CysTyr: 0.287 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	3.862AspAla: 3.862 ± 0.074	0.274AspCys: 0.274 ± 0.022	2.243AspAsp: 2.243 ± 0.07	4.032AspGlu: 4.032 ± 0.086	1.914AspPhe: 1.914 ± 0.049	3.733AspGly: 3.733 ± 0.078	1.044AspHis: 1.044 ± 0.037	2.651AspIle: 2.651 ± 0.07	1.165AspLys: 1.165 ± 0.039	6.045AspLeu: 6.045 ± 0.093	0.91AspMet: 0.91 ± 0.033	0.804AspAsn: 0.804 ± 0.047	3.605AspPro: 3.605 ± 0.073	1.078AspGln: 1.078 ± 0.038	3.642AspArg: 3.642 ± 0.069	1.743AspSer: 1.743 ± 0.046	2.27AspThr: 2.27 ± 0.066	4.006AspVal: 4.006 ± 0.087	0.732AspTrp: 0.732 ± 0.033	1.798AspTyr: 1.798 ± 0.057	0.0AspXaa: 0.0 ± 0.0
Glu	8.111GluAla: 8.111 ± 0.13	0.455GluCys: 0.455 ± 0.027	4.339GluAsp: 4.339 ± 0.078	10.453GluGlu: 10.453 ± 0.211	2.455GluPhe: 2.455 ± 0.056	8.446GluGly: 8.446 ± 0.121	1.701GluHis: 1.701 ± 0.047	4.318GluIle: 4.318 ± 0.086	3.972GluLys: 3.972 ± 0.096	6.561GluLeu: 6.561 ± 0.113	1.752GluMet: 1.752 ± 0.048	1.687GluAsn: 1.687 ± 0.053	2.512GluPro: 2.512 ± 0.069	2.456GluGln: 2.456 ± 0.062	7.209GluArg: 7.209 ± 0.139	2.758GluSer: 2.758 ± 0.061	3.599GluThr: 3.599 ± 0.07	7.464GluVal: 7.464 ± 0.139	1.128GluTrp: 1.128 ± 0.046	2.268GluTyr: 2.268 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	2.826PheAla: 2.826 ± 0.065	0.402PheCys: 0.402 ± 0.024	2.068PheAsp: 2.068 ± 0.049	2.559PheGlu: 2.559 ± 0.058	2.337PhePhe: 2.337 ± 0.065	2.876PheGly: 2.876 ± 0.068	1.009PheHis: 1.009 ± 0.034	1.899PheIle: 1.899 ± 0.055	1.17PheLys: 1.17 ± 0.038	5.704PheLeu: 5.704 ± 0.122	0.785PheMet: 0.785 ± 0.035	0.924PheAsn: 0.924 ± 0.039	2.541PhePro: 2.541 ± 0.061	1.231PheGln: 1.231 ± 0.04	2.715PheArg: 2.715 ± 0.058	3.416PheSer: 3.416 ± 0.074	2.37PheThr: 2.37 ± 0.06	3.014PheVal: 3.014 ± 0.071	0.599PheTrp: 0.599 ± 0.032	1.383PheTyr: 1.383 ± 0.046	0.0PheXaa: 0.0 ± 0.0
Gly	6.255GlyAla: 6.255 ± 0.101	0.807GlyCys: 0.807 ± 0.034	3.739GlyAsp: 3.739 ± 0.074	6.477GlyGlu: 6.477 ± 0.103	3.712GlyPhe: 3.712 ± 0.076	7.024GlyGly: 7.024 ± 0.143	1.385GlyHis: 1.385 ± 0.04	5.329GlyIle: 5.329 ± 0.086	4.058GlyLys: 4.058 ± 0.081	8.162GlyLeu: 8.162 ± 0.129	2.403GlyMet: 2.403 ± 0.058	1.79GlyAsn: 1.79 ± 0.053	2.787GlyPro: 2.787 ± 0.066	1.605GlyGln: 1.605 ± 0.053	6.173GlyArg: 6.173 ± 0.11	4.075GlySer: 4.075 ± 0.094	4.495GlyThr: 4.495 ± 0.094	7.503GlyVal: 7.503 ± 0.129	1.346GlyTrp: 1.346 ± 0.047	2.75GlyTyr: 2.75 ± 0.065	0.0GlyXaa: 0.0 ± 0.0
His	1.738HisAla: 1.738 ± 0.049	0.185HisCys: 0.185 ± 0.014	0.883HisAsp: 0.883 ± 0.033	1.449HisGlu: 1.449 ± 0.044	0.792HisPhe: 0.792 ± 0.033	1.623HisGly: 1.623 ± 0.047	0.642HisHis: 0.642 ± 0.04	1.204HisIle: 1.204 ± 0.042	0.547HisLys: 0.547 ± 0.029	2.925HisLeu: 2.925 ± 0.067	0.439HisMet: 0.439 ± 0.023	0.497HisAsn: 0.497 ± 0.026	1.832HisPro: 1.832 ± 0.059	0.535HisGln: 0.535 ± 0.027	1.664HisArg: 1.664 ± 0.04	0.814HisSer: 0.814 ± 0.031	1.426HisThr: 1.426 ± 0.049	1.593HisVal: 1.593 ± 0.047	0.226HisTrp: 0.226 ± 0.017	0.783HisTyr: 0.783 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	4.427IleAla: 4.427 ± 0.088	0.398IleCys: 0.398 ± 0.021	2.74IleAsp: 2.74 ± 0.077	3.876IleGlu: 3.876 ± 0.083	2.025IlePhe: 2.025 ± 0.052	3.626IleGly: 3.626 ± 0.082	1.336IleHis: 1.336 ± 0.047	2.498IleIle: 2.498 ± 0.069	1.685IleLys: 1.685 ± 0.058	6.348IleLeu: 6.348 ± 0.115	0.874IleMet: 0.874 ± 0.037	1.161IleAsn: 1.161 ± 0.044	3.768IlePro: 3.768 ± 0.077	1.691IleGln: 1.691 ± 0.048	3.911IleArg: 3.911 ± 0.069	3.188IleSer: 3.188 ± 0.066	3.042IleThr: 3.042 ± 0.074	3.746IleVal: 3.746 ± 0.077	0.504IleTrp: 0.504 ± 0.028	1.47IleTyr: 1.47 ± 0.054	0.0IleXaa: 0.0 ± 0.0
Lys	3.713LysAla: 3.713 ± 0.088	0.174LysCys: 0.174 ± 0.015	2.186LysAsp: 2.186 ± 0.054	4.351LysGlu: 4.351 ± 0.101	0.809LysPhe: 0.809 ± 0.033	3.4LysGly: 3.4 ± 0.069	0.572LysHis: 0.572 ± 0.027	1.999LysIle: 1.999 ± 0.059	2.371LysLys: 2.371 ± 0.082	2.791LysLeu: 2.791 ± 0.073	0.755LysMet: 0.755 ± 0.033	0.983LysAsn: 0.983 ± 0.036	1.468LysPro: 1.468 ± 0.05	1.011LysGln: 1.011 ± 0.04	2.909LysArg: 2.909 ± 0.072	1.408LysSer: 1.408 ± 0.041	2.001LysThr: 2.001 ± 0.057	3.215LysVal: 3.215 ± 0.081	0.393LysTrp: 0.393 ± 0.028	0.983LysTyr: 0.983 ± 0.038	0.0LysXaa: 0.0 ± 0.0
Leu	9.487LeuAla: 9.487 ± 0.123	0.88LeuCys: 0.88 ± 0.038	5.011LeuAsp: 5.011 ± 0.074	8.446LeuGlu: 8.446 ± 0.126	5.184LeuPhe: 5.184 ± 0.103	8.048LeuGly: 8.048 ± 0.122	2.273LeuHis: 2.273 ± 0.059	5.096LeuIle: 5.096 ± 0.104	4.212LeuLys: 4.212 ± 0.082	13.773LeuLeu: 13.773 ± 0.252	2.201LeuMet: 2.201 ± 0.06	2.116LeuAsn: 2.116 ± 0.053	6.307LeuPro: 6.307 ± 0.118	2.868LeuGln: 2.868 ± 0.064	7.254LeuArg: 7.254 ± 0.12	8.892LeuSer: 8.892 ± 0.147	5.621LeuThr: 5.621 ± 0.127	9.233LeuVal: 9.233 ± 0.131	1.498LeuTrp: 1.498 ± 0.045	3.381LeuTyr: 3.381 ± 0.071	0.0LeuXaa: 0.0 ± 0.0
Met	1.814MetAla: 1.814 ± 0.049	0.17MetCys: 0.17 ± 0.013	1.137MetAsp: 1.137 ± 0.037	2.119MetGlu: 2.119 ± 0.053	0.591MetPhe: 0.591 ± 0.031	2.051MetGly: 2.051 ± 0.057	0.351MetHis: 0.351 ± 0.02	0.967MetIle: 0.967 ± 0.036	1.297MetLys: 1.297 ± 0.044	1.833MetLeu: 1.833 ± 0.048	0.466MetMet: 0.466 ± 0.025	0.669MetAsn: 0.669 ± 0.03	0.854MetPro: 0.854 ± 0.039	0.537MetGln: 0.537 ± 0.027	1.914MetArg: 1.914 ± 0.05	1.132MetSer: 1.132 ± 0.038	0.874MetThr: 0.874 ± 0.029	1.911MetVal: 1.911 ± 0.052	0.256MetTrp: 0.256 ± 0.017	0.453MetTyr: 0.453 ± 0.025	0.0MetXaa: 0.0 ± 0.0
Asn	1.696AsnAla: 1.696 ± 0.045	0.176AsnCys: 0.176 ± 0.017	0.894AsnAsp: 0.894 ± 0.034	1.346AsnGlu: 1.346 ± 0.047	0.804AsnPhe: 0.804 ± 0.033	1.584AsnGly: 1.584 ± 0.049	0.384AsnHis: 0.384 ± 0.025	1.359AsnIle: 1.359 ± 0.046	0.653AsnLys: 0.653 ± 0.032	2.575AsnLeu: 2.575 ± 0.064	0.471AsnMet: 0.471 ± 0.027	0.587AsnAsn: 0.587 ± 0.027	1.625AsnPro: 1.625 ± 0.046	0.596AsnGln: 0.596 ± 0.03	1.529AsnArg: 1.529 ± 0.048	0.83AsnSer: 0.83 ± 0.039	1.18AsnThr: 1.18 ± 0.046	1.664AsnVal: 1.664 ± 0.047	0.292AsnTrp: 0.292 ± 0.023	0.666AsnTyr: 0.666 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	3.693ProAla: 3.693 ± 0.081	0.355ProCys: 0.355 ± 0.019	3.021ProAsp: 3.021 ± 0.059	5.157ProGlu: 5.157 ± 0.087	2.338ProPhe: 2.338 ± 0.057	4.255ProGly: 4.255 ± 0.082	1.535ProHis: 1.535 ± 0.051	1.975ProIle: 1.975 ± 0.051	1.082ProLys: 1.082 ± 0.044	5.856ProLeu: 5.856 ± 0.109	0.84ProMet: 0.84 ± 0.032	0.936ProAsn: 0.936 ± 0.039	3.16ProPro: 3.16 ± 0.089	1.488ProGln: 1.488 ± 0.039	3.132ProArg: 3.132 ± 0.066	3.698ProSer: 3.698 ± 0.077	2.276ProThr: 2.276 ± 0.082	4.449ProVal: 4.449 ± 0.077	0.776ProTrp: 0.776 ± 0.03	1.8ProTyr: 1.8 ± 0.053	0.0ProXaa: 0.0 ± 0.0
Gln	2.322GlnAla: 2.322 ± 0.055	0.167GlnCys: 0.167 ± 0.015	1.313GlnAsp: 1.313 ± 0.043	2.811GlnGlu: 2.811 ± 0.068	0.825GlnPhe: 0.825 ± 0.034	2.164GlnGly: 2.164 ± 0.058	0.581GlnHis: 0.581 ± 0.028	1.615GlnIle: 1.615 ± 0.051	1.39GlnLys: 1.39 ± 0.045	2.097GlnLeu: 2.097 ± 0.061	0.648GlnMet: 0.648 ± 0.029	0.66GlnAsn: 0.66 ± 0.035	0.892GlnPro: 0.892 ± 0.038	0.885GlnGln: 0.885 ± 0.04	2.027GlnArg: 2.027 ± 0.042	1.04GlnSer: 1.04 ± 0.036	1.229GlnThr: 1.229 ± 0.041	2.401GlnVal: 2.401 ± 0.063	0.317GlnTrp: 0.317 ± 0.02	0.811GlnTyr: 0.811 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	5.81ArgAla: 5.81 ± 0.103	0.622ArgCys: 0.622 ± 0.032	3.364ArgAsp: 3.364 ± 0.073	7.482ArgGlu: 7.482 ± 0.127	3.172ArgPhe: 3.172 ± 0.069	5.525ArgGly: 5.525 ± 0.096	1.541ArgHis: 1.541 ± 0.043	5.17ArgIle: 5.17 ± 0.088	3.395ArgLys: 3.395 ± 0.081	8.136ArgLeu: 8.136 ± 0.121	2.155ArgMet: 2.155 ± 0.063	1.702ArgAsn: 1.702 ± 0.055	3.09ArgPro: 3.09 ± 0.067	1.65ArgGln: 1.65 ± 0.051	6.403ArgArg: 6.403 ± 0.114	3.528ArgSer: 3.528 ± 0.066	3.936ArgThr: 3.936 ± 0.082	6.082ArgVal: 6.082 ± 0.118	1.162ArgTrp: 1.162 ± 0.041	2.749ArgTyr: 2.749 ± 0.062	0.0ArgXaa: 0.0 ± 0.0
Ser	3.793SerAla: 3.793 ± 0.085	0.553SerCys: 0.553 ± 0.027	2.245SerAsp: 2.245 ± 0.057	3.164SerGlu: 3.164 ± 0.062	3.0SerPhe: 3.0 ± 0.067	4.545SerGly: 4.545 ± 0.095	1.34SerHis: 1.34 ± 0.043	3.005SerIle: 3.005 ± 0.06	1.408SerLys: 1.408 ± 0.05	7.439SerLeu: 7.439 ± 0.118	1.184SerMet: 1.184 ± 0.038	0.976SerAsn: 0.976 ± 0.039	3.585SerPro: 3.585 ± 0.086	1.474SerGln: 1.474 ± 0.049	4.373SerArg: 4.373 ± 0.085	3.985SerSer: 3.985 ± 0.09	2.715SerThr: 2.715 ± 0.063	3.736SerVal: 3.736 ± 0.067	0.879SerTrp: 0.879 ± 0.037	1.968SerTyr: 1.968 ± 0.057	0.0SerXaa: 0.0 ± 0.0
Thr	3.831ThrAla: 3.831 ± 0.073	0.398ThrCys: 0.398 ± 0.021	2.084ThrAsp: 2.084 ± 0.055	2.857ThrGlu: 2.857 ± 0.06	2.055ThrPhe: 2.055 ± 0.059	4.622ThrGly: 4.622 ± 0.095	1.222ThrHis: 1.222 ± 0.04	2.938ThrIle: 2.938 ± 0.075	1.364ThrLys: 1.364 ± 0.048	5.938ThrLeu: 5.938 ± 0.114	1.044ThrMet: 1.044 ± 0.036	0.996ThrAsn: 0.996 ± 0.038	3.471ThrPro: 3.471 ± 0.103	1.16ThrGln: 1.16 ± 0.045	3.319ThrArg: 3.319 ± 0.061	2.905ThrSer: 2.905 ± 0.065	2.79ThrThr: 2.79 ± 0.084	3.985ThrVal: 3.985 ± 0.092	0.743ThrTrp: 0.743 ± 0.039	1.798ThrTyr: 1.798 ± 0.051	0.0ThrXaa: 0.0 ± 0.0
Val	6.153ValAla: 6.153 ± 0.093	0.896ValCys: 0.896 ± 0.039	4.114ValAsp: 4.114 ± 0.071	7.112ValGlu: 7.112 ± 0.126	3.693ValPhe: 3.693 ± 0.074	6.451ValGly: 6.451 ± 0.116	1.663ValHis: 1.663 ± 0.049	4.068ValIle: 4.068 ± 0.082	3.343ValLys: 3.343 ± 0.078	9.366ValLeu: 9.366 ± 0.13	1.692ValMet: 1.692 ± 0.045	1.662ValAsn: 1.662 ± 0.044	4.205ValPro: 4.205 ± 0.077	2.138ValGln: 2.138 ± 0.066	6.829ValArg: 6.829 ± 0.108	4.62ValSer: 4.62 ± 0.087	3.381ValThr: 3.381 ± 0.074	8.165ValVal: 8.165 ± 0.143	1.365ValTrp: 1.365 ± 0.062	2.525ValTyr: 2.525 ± 0.065	0.0ValXaa: 0.0 ± 0.0
Trp	1.116TrpAla: 1.116 ± 0.04	0.132TrpCys: 0.132 ± 0.013	0.745TrpAsp: 0.745 ± 0.035	1.376TrpGlu: 1.376 ± 0.047	0.622TrpPhe: 0.622 ± 0.031	1.286TrpGly: 1.286 ± 0.046	0.325TrpHis: 0.325 ± 0.021	0.7TrpIle: 0.7 ± 0.027	0.809TrpLys: 0.809 ± 0.036	1.182TrpLeu: 1.182 ± 0.045	0.37TrpMet: 0.37 ± 0.021	0.45TrpAsn: 0.45 ± 0.023	0.414TrpPro: 0.414 ± 0.024	0.356TrpGln: 0.356 ± 0.024	1.005TrpArg: 1.005 ± 0.04	0.63TrpSer: 0.63 ± 0.033	0.566TrpThr: 0.566 ± 0.028	1.208TrpVal: 1.208 ± 0.05	0.355TrpTrp: 0.355 ± 0.022	0.586TrpTyr: 0.586 ± 0.03	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.618TyrAla: 2.618 ± 0.063	0.266TyrCys: 0.266 ± 0.018	1.65TyrAsp: 1.65 ± 0.055	2.347TyrGlu: 2.347 ± 0.061	1.406TyrPhe: 1.406 ± 0.04	2.53TyrGly: 2.53 ± 0.056	0.804TyrHis: 0.804 ± 0.034	1.392TyrIle: 1.392 ± 0.047	0.974TyrLys: 0.974 ± 0.037	3.969TyrLeu: 3.969 ± 0.078	0.553TyrMet: 0.553 ± 0.023	0.765TyrAsn: 0.765 ± 0.029	1.738TyrPro: 1.738 ± 0.049	0.955TyrGln: 0.955 ± 0.034	3.01TyrArg: 3.01 ± 0.062	1.48TyrSer: 1.48 ± 0.05	1.937TyrThr: 1.937 ± 0.056	2.42TyrVal: 2.42 ± 0.057	0.472TyrTrp: 0.472 ± 0.029	1.234TyrTyr: 1.234 ± 0.051	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2249 proteins (788271 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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