Escherichia phage vB_EcoS_NBD2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Vilniusvirus; Escherichia virus NBD2

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 87 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A192Y7P2|A0A192Y7P2_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_NBD2 OX=1852563 GN=NBD2_22 PE=4 SV=1
MM1 pKa = 7.51SMNAIANITVDD12 pKa = 4.42LKK14 pKa = 11.53AKK16 pKa = 9.38IQYY19 pKa = 7.4TIPEE23 pKa = 4.28SGTFLFAWAYY33 pKa = 10.37RR34 pKa = 11.84DD35 pKa = 3.49QPFAATYY42 pKa = 7.24QWRR45 pKa = 11.84SGEE48 pKa = 4.11LAVMTDD54 pKa = 2.76SGYY57 pKa = 11.08EE58 pKa = 3.74IAGVGAAEE66 pKa = 4.51EE67 pKa = 4.89YY68 pKa = 11.09YY69 pKa = 10.15PLPDD73 pKa = 3.83LNRR76 pKa = 11.84IVAGEE81 pKa = 3.93DD82 pKa = 3.15TASKK86 pKa = 10.12FIAIVVIDD94 pKa = 3.76AAA96 pKa = 4.37

Molecular weight: 10.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A192Y9G5|A0A192Y9G5_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoS_NBD2 OX=1852563 GN=NBD2_13 PE=4 SV=1
MM1 pKa = 7.31TVTFPSFTPLASGTPWKK18 pKa = 10.39LLKK21 pKa = 10.57SWILPSRR28 pKa = 11.84PARR31 pKa = 11.84FQPIAMVAHH40 pKa = 6.32FVLAVSAATITAIVLRR56 pKa = 11.84AMKK59 pKa = 10.03ARR61 pKa = 11.84CLTRR65 pKa = 11.84SRR67 pKa = 11.84GASARR72 pKa = 11.84RR73 pKa = 11.84IIFALISTTANALAQSVARR92 pKa = 11.84LATT95 pKa = 3.56

Molecular weight: 10.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

87

0

87

15944

47

1200

183.3

20.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.195 ± 0.474

1.399 ± 0.151

5.896 ± 0.268

6.51 ± 0.226

4.027 ± 0.193

7.62 ± 0.193

1.888 ± 0.185

6.247 ± 0.195

6.943 ± 0.248

6.874 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.029 ± 0.164

4.616 ± 0.249

3.387 ± 0.191

3.682 ± 0.219

5.137 ± 0.206

6.147 ± 0.256

5.871 ± 0.286

6.548 ± 0.252

1.505 ± 0.127

3.481 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski