Amino acid dipepetide frequency for Escherichia phage vB_EcoS_NBD2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.032AlaAla: 9.032 ± 1.408
0.941AlaCys: 0.941 ± 0.268
4.391AlaAsp: 4.391 ± 0.494
5.959AlaGlu: 5.959 ± 0.874
3.387AlaPhe: 3.387 ± 0.48
7.15AlaGly: 7.15 ± 0.687
1.631AlaHis: 1.631 ± 0.379
5.645AlaIle: 5.645 ± 0.576
6.461AlaLys: 6.461 ± 0.942
7.401AlaLeu: 7.401 ± 0.842
2.572AlaMet: 2.572 ± 0.425
4.642AlaAsn: 4.642 ± 0.725
2.383AlaPro: 2.383 ± 0.384
4.077AlaGln: 4.077 ± 0.587
4.767AlaArg: 4.767 ± 0.569
6.398AlaSer: 6.398 ± 0.935
5.331AlaThr: 5.331 ± 0.795
5.645AlaVal: 5.645 ± 0.814
1.38AlaTrp: 1.38 ± 0.262
2.76AlaTyr: 2.76 ± 0.541
0.0AlaXaa: 0.0 ± 0.0
Cys
1.317CysAla: 1.317 ± 0.311
0.314CysCys: 0.314 ± 0.148
1.317CysAsp: 1.317 ± 0.244
0.815CysGlu: 0.815 ± 0.291
0.251CysPhe: 0.251 ± 0.12
1.631CysGly: 1.631 ± 0.362
0.125CysHis: 0.125 ± 0.08
0.941CysIle: 0.941 ± 0.233
0.815CysLys: 0.815 ± 0.261
0.878CysLeu: 0.878 ± 0.223
0.502CysMet: 0.502 ± 0.17
0.565CysAsn: 0.565 ± 0.208
0.565CysPro: 0.565 ± 0.194
0.188CysGln: 0.188 ± 0.112
0.941CysArg: 0.941 ± 0.269
0.502CysSer: 0.502 ± 0.186
0.753CysThr: 0.753 ± 0.237
0.941CysVal: 0.941 ± 0.271
0.251CysTrp: 0.251 ± 0.108
0.376CysTyr: 0.376 ± 0.152
0.0CysXaa: 0.0 ± 0.0
Asp
5.018AspAla: 5.018 ± 0.554
0.753AspCys: 0.753 ± 0.227
3.575AspAsp: 3.575 ± 0.542
4.579AspGlu: 4.579 ± 0.415
2.885AspPhe: 2.885 ± 0.496
5.896AspGly: 5.896 ± 0.646
0.753AspHis: 0.753 ± 0.192
2.823AspIle: 2.823 ± 0.374
3.45AspLys: 3.45 ± 0.478
4.14AspLeu: 4.14 ± 0.532
1.568AspMet: 1.568 ± 0.285
2.321AspAsn: 2.321 ± 0.511
2.007AspPro: 2.007 ± 0.335
2.07AspGln: 2.07 ± 0.35
2.572AspArg: 2.572 ± 0.378
3.638AspSer: 3.638 ± 0.471
2.572AspThr: 2.572 ± 0.419
4.202AspVal: 4.202 ± 0.532
0.941AspTrp: 0.941 ± 0.194
3.199AspTyr: 3.199 ± 0.451
0.0AspXaa: 0.0 ± 0.0
Glu
6.084GluAla: 6.084 ± 0.511
0.941GluCys: 0.941 ± 0.233
3.262GluAsp: 3.262 ± 0.481
4.642GluGlu: 4.642 ± 0.536
3.136GluPhe: 3.136 ± 0.362
4.014GluGly: 4.014 ± 0.405
1.066GluHis: 1.066 ± 0.298
4.892GluIle: 4.892 ± 0.534
4.077GluLys: 4.077 ± 0.521
4.83GluLeu: 4.83 ± 0.548
2.572GluMet: 2.572 ± 0.467
2.948GluAsn: 2.948 ± 0.431
1.944GluPro: 1.944 ± 0.37
3.136GluGln: 3.136 ± 0.408
3.199GluArg: 3.199 ± 0.385
3.45GluSer: 3.45 ± 0.515
3.324GluThr: 3.324 ± 0.684
4.453GluVal: 4.453 ± 0.563
0.69GluTrp: 0.69 ± 0.247
2.446GluTyr: 2.446 ± 0.384
0.0GluXaa: 0.0 ± 0.0
Phe
3.324PheAla: 3.324 ± 0.501
0.565PheCys: 0.565 ± 0.171
3.073PheAsp: 3.073 ± 0.41
2.509PheGlu: 2.509 ± 0.481
1.254PhePhe: 1.254 ± 0.295
3.199PheGly: 3.199 ± 0.446
0.753PheHis: 0.753 ± 0.25
2.133PheIle: 2.133 ± 0.402
2.948PheLys: 2.948 ± 0.439
2.446PheLeu: 2.446 ± 0.454
1.004PheMet: 1.004 ± 0.28
2.07PheAsn: 2.07 ± 0.353
1.443PhePro: 1.443 ± 0.286
1.568PheGln: 1.568 ± 0.362
1.443PheArg: 1.443 ± 0.281
2.509PheSer: 2.509 ± 0.459
2.634PheThr: 2.634 ± 0.451
3.324PheVal: 3.324 ± 0.441
1.066PheTrp: 1.066 ± 0.282
1.004PheTyr: 1.004 ± 0.249
0.0PheXaa: 0.0 ± 0.0
Gly
7.59GlyAla: 7.59 ± 0.862
0.941GlyCys: 0.941 ± 0.194
3.763GlyAsp: 3.763 ± 0.468
5.394GlyGlu: 5.394 ± 0.543
3.575GlyPhe: 3.575 ± 0.625
6.461GlyGly: 6.461 ± 0.977
1.443GlyHis: 1.443 ± 0.397
3.889GlyIle: 3.889 ± 0.486
6.711GlyLys: 6.711 ± 0.602
5.457GlyLeu: 5.457 ± 0.541
2.697GlyMet: 2.697 ± 0.479
4.328GlyAsn: 4.328 ± 0.546
0.063GlyPro: 0.063 ± 0.075
1.568GlyGln: 1.568 ± 0.289
3.011GlyArg: 3.011 ± 0.45
5.457GlySer: 5.457 ± 0.536
4.328GlyThr: 4.328 ± 0.682
5.582GlyVal: 5.582 ± 0.604
1.443GlyTrp: 1.443 ± 0.275
2.509GlyTyr: 2.509 ± 0.409
0.0GlyXaa: 0.0 ± 0.0
His
1.192HisAla: 1.192 ± 0.274
0.251HisCys: 0.251 ± 0.15
1.317HisAsp: 1.317 ± 0.293
1.192HisGlu: 1.192 ± 0.323
0.941HisPhe: 0.941 ± 0.231
1.505HisGly: 1.505 ± 0.371
0.627HisHis: 0.627 ± 0.216
1.004HisIle: 1.004 ± 0.312
1.317HisLys: 1.317 ± 0.368
1.505HisLeu: 1.505 ± 0.283
0.251HisMet: 0.251 ± 0.107
0.502HisAsn: 0.502 ± 0.154
0.878HisPro: 0.878 ± 0.229
0.69HisGln: 0.69 ± 0.222
1.317HisArg: 1.317 ± 0.322
0.941HisSer: 0.941 ± 0.217
1.317HisThr: 1.317 ± 0.305
0.878HisVal: 0.878 ± 0.239
0.502HisTrp: 0.502 ± 0.147
0.753HisTyr: 0.753 ± 0.243
0.0HisXaa: 0.0 ± 0.0
Ile
5.457IleAla: 5.457 ± 0.583
1.004IleCys: 1.004 ± 0.329
4.202IleAsp: 4.202 ± 0.542
4.704IleGlu: 4.704 ± 0.48
2.007IlePhe: 2.007 ± 0.351
3.324IleGly: 3.324 ± 0.458
1.631IleHis: 1.631 ± 0.343
3.763IleIle: 3.763 ± 0.559
5.018IleLys: 5.018 ± 0.659
3.136IleLeu: 3.136 ± 0.429
2.07IleMet: 2.07 ± 0.397
2.634IleAsn: 2.634 ± 0.437
2.007IlePro: 2.007 ± 0.337
2.133IleGln: 2.133 ± 0.329
3.513IleArg: 3.513 ± 0.514
3.513IleSer: 3.513 ± 0.451
5.269IleThr: 5.269 ± 0.492
4.202IleVal: 4.202 ± 0.379
1.066IleTrp: 1.066 ± 0.271
1.819IleTyr: 1.819 ± 0.342
0.0IleXaa: 0.0 ± 0.0
Lys
7.652LysAla: 7.652 ± 0.589
0.815LysCys: 0.815 ± 0.224
4.328LysAsp: 4.328 ± 0.432
4.014LysGlu: 4.014 ± 0.478
2.321LysPhe: 2.321 ± 0.384
3.638LysGly: 3.638 ± 0.422
1.568LysHis: 1.568 ± 0.383
4.328LysIle: 4.328 ± 0.419
5.081LysLys: 5.081 ± 0.599
5.206LysLeu: 5.206 ± 0.513
3.262LysMet: 3.262 ± 0.516
2.509LysAsn: 2.509 ± 0.422
2.885LysPro: 2.885 ± 0.434
2.383LysGln: 2.383 ± 0.426
4.14LysArg: 4.14 ± 0.658
4.077LysSer: 4.077 ± 0.659
3.701LysThr: 3.701 ± 0.51
4.579LysVal: 4.579 ± 0.535
1.004LysTrp: 1.004 ± 0.275
1.944LysTyr: 1.944 ± 0.258
0.0LysXaa: 0.0 ± 0.0
Leu
6.649LeuAla: 6.649 ± 0.642
1.066LeuCys: 1.066 ± 0.274
3.952LeuAsp: 3.952 ± 0.497
3.701LeuGlu: 3.701 ± 0.487
2.383LeuPhe: 2.383 ± 0.432
5.457LeuGly: 5.457 ± 0.592
1.254LeuHis: 1.254 ± 0.309
4.14LeuIle: 4.14 ± 0.619
5.394LeuLys: 5.394 ± 0.56
4.014LeuLeu: 4.014 ± 0.414
1.631LeuMet: 1.631 ± 0.335
3.513LeuAsn: 3.513 ± 0.582
3.011LeuPro: 3.011 ± 0.322
1.944LeuGln: 1.944 ± 0.375
3.575LeuArg: 3.575 ± 0.377
4.453LeuSer: 4.453 ± 0.497
5.143LeuThr: 5.143 ± 0.478
4.328LeuVal: 4.328 ± 0.341
1.066LeuTrp: 1.066 ± 0.218
2.07LeuTyr: 2.07 ± 0.268
0.0LeuXaa: 0.0 ± 0.0
Met
2.697MetAla: 2.697 ± 0.417
0.188MetCys: 0.188 ± 0.108
1.004MetAsp: 1.004 ± 0.25
1.192MetGlu: 1.192 ± 0.263
1.066MetPhe: 1.066 ± 0.301
1.317MetGly: 1.317 ± 0.308
0.314MetHis: 0.314 ± 0.13
2.76MetIle: 2.76 ± 0.387
3.262MetLys: 3.262 ± 0.518
2.321MetLeu: 2.321 ± 0.397
1.066MetMet: 1.066 ± 0.351
1.443MetAsn: 1.443 ± 0.269
1.004MetPro: 1.004 ± 0.225
1.631MetGln: 1.631 ± 0.267
2.133MetArg: 2.133 ± 0.352
2.07MetSer: 2.07 ± 0.296
1.694MetThr: 1.694 ± 0.411
1.756MetVal: 1.756 ± 0.338
0.376MetTrp: 0.376 ± 0.165
1.004MetTyr: 1.004 ± 0.217
0.0MetXaa: 0.0 ± 0.0
Asn
4.767AsnAla: 4.767 ± 0.81
0.439AsnCys: 0.439 ± 0.152
2.572AsnAsp: 2.572 ± 0.389
2.76AsnGlu: 2.76 ± 0.401
1.694AsnPhe: 1.694 ± 0.253
4.83AsnGly: 4.83 ± 0.657
0.753AsnHis: 0.753 ± 0.215
2.885AsnIle: 2.885 ± 0.498
2.76AsnLys: 2.76 ± 0.387
3.011AsnLeu: 3.011 ± 0.487
1.254AsnMet: 1.254 ± 0.305
2.258AsnAsn: 2.258 ± 0.49
2.634AsnPro: 2.634 ± 0.38
1.944AsnGln: 1.944 ± 0.349
2.383AsnArg: 2.383 ± 0.348
2.383AsnSer: 2.383 ± 0.463
1.756AsnThr: 1.756 ± 0.279
2.948AsnVal: 2.948 ± 0.42
0.565AsnTrp: 0.565 ± 0.177
1.568AsnTyr: 1.568 ± 0.31
0.0AsnXaa: 0.0 ± 0.0
Pro
2.509ProAla: 2.509 ± 0.358
0.439ProCys: 0.439 ± 0.141
2.823ProAsp: 2.823 ± 0.397
3.262ProGlu: 3.262 ± 0.639
2.007ProPhe: 2.007 ± 0.375
2.321ProGly: 2.321 ± 0.4
0.753ProHis: 0.753 ± 0.222
1.631ProIle: 1.631 ± 0.433
2.133ProLys: 2.133 ± 0.342
2.07ProLeu: 2.07 ± 0.354
0.627ProMet: 0.627 ± 0.18
1.505ProAsn: 1.505 ± 0.235
0.941ProPro: 0.941 ± 0.208
1.004ProGln: 1.004 ± 0.269
1.443ProArg: 1.443 ± 0.3
2.07ProSer: 2.07 ± 0.341
1.819ProThr: 1.819 ± 0.295
2.634ProVal: 2.634 ± 0.359
0.69ProTrp: 0.69 ± 0.194
1.192ProTyr: 1.192 ± 0.285
0.0ProXaa: 0.0 ± 0.0
Gln
3.513GlnAla: 3.513 ± 0.602
0.188GlnCys: 0.188 ± 0.106
1.631GlnAsp: 1.631 ± 0.318
1.819GlnGlu: 1.819 ± 0.269
1.004GlnPhe: 1.004 ± 0.252
2.258GlnGly: 2.258 ± 0.409
0.69GlnHis: 0.69 ± 0.175
2.76GlnIle: 2.76 ± 0.385
1.756GlnLys: 1.756 ± 0.402
3.073GlnLeu: 3.073 ± 0.426
1.38GlnMet: 1.38 ± 0.288
1.631GlnAsn: 1.631 ± 0.353
1.631GlnPro: 1.631 ± 0.346
2.07GlnGln: 2.07 ± 0.576
2.195GlnArg: 2.195 ± 0.403
2.195GlnSer: 2.195 ± 0.396
2.383GlnThr: 2.383 ± 0.375
2.446GlnVal: 2.446 ± 0.398
0.565GlnTrp: 0.565 ± 0.226
1.631GlnTyr: 1.631 ± 0.324
0.0GlnXaa: 0.0 ± 0.0
Arg
4.579ArgAla: 4.579 ± 0.647
1.254ArgCys: 1.254 ± 0.311
2.572ArgAsp: 2.572 ± 0.373
3.952ArgGlu: 3.952 ± 0.479
2.509ArgPhe: 2.509 ± 0.362
3.952ArgGly: 3.952 ± 0.432
0.878ArgHis: 0.878 ± 0.284
3.262ArgIle: 3.262 ± 0.431
4.077ArgLys: 4.077 ± 0.416
3.324ArgLeu: 3.324 ± 0.361
1.443ArgMet: 1.443 ± 0.29
2.195ArgAsn: 2.195 ± 0.373
2.07ArgPro: 2.07 ± 0.364
1.819ArgGln: 1.819 ± 0.363
2.885ArgArg: 2.885 ± 0.4
2.76ArgSer: 2.76 ± 0.412
2.195ArgThr: 2.195 ± 0.376
3.262ArgVal: 3.262 ± 0.697
0.565ArgTrp: 0.565 ± 0.216
1.819ArgTyr: 1.819 ± 0.348
0.0ArgXaa: 0.0 ± 0.0
Ser
5.645SerAla: 5.645 ± 0.854
0.815SerCys: 0.815 ± 0.328
4.14SerAsp: 4.14 ± 0.458
4.265SerGlu: 4.265 ± 0.562
2.823SerPhe: 2.823 ± 0.439
6.272SerGly: 6.272 ± 0.58
1.004SerHis: 1.004 ± 0.323
3.638SerIle: 3.638 ± 0.598
3.952SerLys: 3.952 ± 0.53
4.955SerLeu: 4.955 ± 0.481
1.568SerMet: 1.568 ± 0.347
2.133SerAsn: 2.133 ± 0.379
1.819SerPro: 1.819 ± 0.335
2.823SerGln: 2.823 ± 0.382
3.136SerArg: 3.136 ± 0.399
2.76SerSer: 2.76 ± 0.549
3.324SerThr: 3.324 ± 0.537
3.136SerVal: 3.136 ± 0.503
0.815SerTrp: 0.815 ± 0.194
2.383SerTyr: 2.383 ± 0.367
0.0SerXaa: 0.0 ± 0.0
Thr
5.331ThrAla: 5.331 ± 0.978
0.815ThrCys: 0.815 ± 0.241
2.823ThrAsp: 2.823 ± 0.403
3.575ThrGlu: 3.575 ± 0.414
2.446ThrPhe: 2.446 ± 0.41
4.955ThrGly: 4.955 ± 0.525
1.254ThrHis: 1.254 ± 0.262
4.391ThrIle: 4.391 ± 0.523
3.011ThrLys: 3.011 ± 0.358
3.513ThrLeu: 3.513 ± 0.423
1.756ThrMet: 1.756 ± 0.338
3.262ThrAsn: 3.262 ± 0.567
3.011ThrPro: 3.011 ± 0.458
2.446ThrGln: 2.446 ± 0.364
2.258ThrArg: 2.258 ± 0.363
4.642ThrSer: 4.642 ± 0.887
2.76ThrThr: 2.76 ± 0.398
3.952ThrVal: 3.952 ± 0.429
0.815ThrTrp: 0.815 ± 0.184
1.694ThrTyr: 1.694 ± 0.347
0.0ThrXaa: 0.0 ± 0.0
Val
5.645ValAla: 5.645 ± 0.629
1.004ValCys: 1.004 ± 0.266
4.892ValAsp: 4.892 ± 0.5
4.642ValGlu: 4.642 ± 0.503
2.76ValPhe: 2.76 ± 0.424
4.265ValGly: 4.265 ± 0.543
1.254ValHis: 1.254 ± 0.316
4.579ValIle: 4.579 ± 0.64
4.516ValLys: 4.516 ± 0.456
3.826ValLeu: 3.826 ± 0.472
1.819ValMet: 1.819 ± 0.371
3.324ValAsn: 3.324 ± 0.368
2.133ValPro: 2.133 ± 0.414
1.443ValGln: 1.443 ± 0.37
3.575ValArg: 3.575 ± 0.502
4.014ValSer: 4.014 ± 0.424
4.265ValThr: 4.265 ± 0.416
4.14ValVal: 4.14 ± 0.417
0.69ValTrp: 0.69 ± 0.18
2.697ValTyr: 2.697 ± 0.388
0.0ValXaa: 0.0 ± 0.0
Trp
1.004TrpAla: 1.004 ± 0.257
0.314TrpCys: 0.314 ± 0.125
0.753TrpAsp: 0.753 ± 0.2
0.565TrpGlu: 0.565 ± 0.175
0.627TrpPhe: 0.627 ± 0.217
1.129TrpGly: 1.129 ± 0.266
0.565TrpHis: 0.565 ± 0.162
1.254TrpIle: 1.254 ± 0.325
1.317TrpLys: 1.317 ± 0.228
1.443TrpLeu: 1.443 ± 0.322
0.376TrpMet: 0.376 ± 0.154
0.753TrpAsn: 0.753 ± 0.241
0.188TrpPro: 0.188 ± 0.138
0.376TrpGln: 0.376 ± 0.139
0.941TrpArg: 0.941 ± 0.276
1.004TrpSer: 1.004 ± 0.269
0.878TrpThr: 0.878 ± 0.243
0.815TrpVal: 0.815 ± 0.213
0.188TrpTrp: 0.188 ± 0.103
0.565TrpTyr: 0.565 ± 0.215
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.948TyrAla: 2.948 ± 0.522
0.941TyrCys: 0.941 ± 0.212
2.572TyrAsp: 2.572 ± 0.416
1.568TyrGlu: 1.568 ± 0.284
1.192TyrPhe: 1.192 ± 0.265
2.634TyrGly: 2.634 ± 0.33
0.565TyrHis: 0.565 ± 0.18
1.756TyrIle: 1.756 ± 0.345
1.38TyrLys: 1.38 ± 0.301
2.195TyrLeu: 2.195 ± 0.367
0.815TyrMet: 0.815 ± 0.228
1.631TyrAsn: 1.631 ± 0.349
1.254TyrPro: 1.254 ± 0.239
1.505TyrGln: 1.505 ± 0.296
1.944TyrArg: 1.944 ± 0.339
2.634TyrSer: 2.634 ± 0.356
3.262TyrThr: 3.262 ± 0.315
2.258TyrVal: 2.258 ± 0.392
0.376TyrTrp: 0.376 ± 0.169
1.38TyrTyr: 1.38 ± 0.337
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 87 proteins (15944 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski