Amino acid dipepetide frequency for Arthrobacter phage Kuleana

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.969AlaAla: 18.969 ± 1.407
0.896AlaCys: 0.896 ± 0.333
8.955AlaAsp: 8.955 ± 0.932
7.327AlaGlu: 7.327 ± 0.665
3.094AlaPhe: 3.094 ± 0.619
15.306AlaGly: 15.306 ± 1.278
1.465AlaHis: 1.465 ± 0.342
4.071AlaIle: 4.071 ± 0.636
2.117AlaLys: 2.117 ± 0.471
11.642AlaLeu: 11.642 ± 1.035
3.338AlaMet: 3.338 ± 0.536
2.931AlaAsn: 2.931 ± 0.488
5.129AlaPro: 5.129 ± 0.737
3.338AlaGln: 3.338 ± 0.484
9.281AlaArg: 9.281 ± 0.875
6.35AlaSer: 6.35 ± 0.707
5.699AlaThr: 5.699 ± 0.659
10.339AlaVal: 10.339 ± 0.938
3.745AlaTrp: 3.745 ± 0.476
2.117AlaTyr: 2.117 ± 0.418
0.0AlaXaa: 0.0 ± 0.0
Cys
0.488CysAla: 0.488 ± 0.188
0.0CysCys: 0.0 ± 0.0
0.244CysAsp: 0.244 ± 0.134
0.488CysGlu: 0.488 ± 0.183
0.0CysPhe: 0.0 ± 0.0
2.035CysGly: 2.035 ± 0.467
0.163CysHis: 0.163 ± 0.12
0.407CysIle: 0.407 ± 0.16
0.488CysLys: 0.488 ± 0.197
0.488CysLeu: 0.488 ± 0.229
0.081CysMet: 0.081 ± 0.085
0.163CysAsn: 0.163 ± 0.105
0.244CysPro: 0.244 ± 0.147
0.407CysGln: 0.407 ± 0.176
1.384CysArg: 1.384 ± 0.35
0.896CysSer: 0.896 ± 0.267
0.488CysThr: 0.488 ± 0.187
0.733CysVal: 0.733 ± 0.256
0.163CysTrp: 0.163 ± 0.108
0.081CysTyr: 0.081 ± 0.081
0.0CysXaa: 0.0 ± 0.0
Asp
5.943AspAla: 5.943 ± 0.622
0.488AspCys: 0.488 ± 0.205
2.931AspAsp: 2.931 ± 0.456
3.826AspGlu: 3.826 ± 0.558
1.71AspPhe: 1.71 ± 0.274
6.513AspGly: 6.513 ± 0.772
1.058AspHis: 1.058 ± 0.302
1.14AspIle: 1.14 ± 0.34
1.791AspLys: 1.791 ± 0.375
6.757AspLeu: 6.757 ± 0.798
1.14AspMet: 1.14 ± 0.447
0.977AspAsn: 0.977 ± 0.235
4.478AspPro: 4.478 ± 0.474
1.303AspGln: 1.303 ± 0.314
3.338AspArg: 3.338 ± 0.479
3.582AspSer: 3.582 ± 0.581
1.873AspThr: 1.873 ± 0.428
5.129AspVal: 5.129 ± 0.705
1.71AspTrp: 1.71 ± 0.368
1.303AspTyr: 1.303 ± 0.36
0.0AspXaa: 0.0 ± 0.0
Glu
6.839GluAla: 6.839 ± 0.754
0.733GluCys: 0.733 ± 0.235
2.687GluAsp: 2.687 ± 0.344
5.048GluGlu: 5.048 ± 0.876
1.303GluPhe: 1.303 ± 0.291
4.559GluGly: 4.559 ± 0.584
1.14GluHis: 1.14 ± 0.359
3.582GluIle: 3.582 ± 0.539
2.198GluLys: 2.198 ± 0.419
6.757GluLeu: 6.757 ± 0.775
0.896GluMet: 0.896 ± 0.253
1.547GluAsn: 1.547 ± 0.376
3.908GluPro: 3.908 ± 0.748
2.768GluGln: 2.768 ± 0.51
5.129GluArg: 5.129 ± 0.71
2.849GluSer: 2.849 ± 0.443
3.501GluThr: 3.501 ± 0.567
6.187GluVal: 6.187 ± 0.892
1.303GluTrp: 1.303 ± 0.27
1.791GluTyr: 1.791 ± 0.294
0.0GluXaa: 0.0 ± 0.0
Phe
2.768PheAla: 2.768 ± 0.46
0.244PheCys: 0.244 ± 0.143
2.361PheAsp: 2.361 ± 0.437
1.954PheGlu: 1.954 ± 0.368
0.733PhePhe: 0.733 ± 0.254
1.873PheGly: 1.873 ± 0.374
0.244PheHis: 0.244 ± 0.151
0.977PheIle: 0.977 ± 0.265
1.71PheLys: 1.71 ± 0.35
2.361PheLeu: 2.361 ± 0.453
0.57PheMet: 0.57 ± 0.164
0.896PheAsn: 0.896 ± 0.276
1.058PhePro: 1.058 ± 0.293
0.407PheGln: 0.407 ± 0.168
2.687PheArg: 2.687 ± 0.436
1.71PheSer: 1.71 ± 0.449
1.303PheThr: 1.303 ± 0.378
1.628PheVal: 1.628 ± 0.291
0.163PheTrp: 0.163 ± 0.097
0.488PheTyr: 0.488 ± 0.213
0.0PheXaa: 0.0 ± 0.0
Gly
12.456GlyAla: 12.456 ± 1.071
0.977GlyCys: 0.977 ± 0.258
5.943GlyAsp: 5.943 ± 0.69
6.187GlyGlu: 6.187 ± 0.629
2.931GlyPhe: 2.931 ± 0.689
10.828GlyGly: 10.828 ± 1.378
1.384GlyHis: 1.384 ± 0.319
3.989GlyIle: 3.989 ± 0.804
3.012GlyLys: 3.012 ± 0.445
8.63GlyLeu: 8.63 ± 1.367
2.687GlyMet: 2.687 ± 0.559
1.71GlyAsn: 1.71 ± 0.377
5.536GlyPro: 5.536 ± 0.743
3.012GlyGln: 3.012 ± 0.435
8.467GlyArg: 8.467 ± 0.837
5.699GlySer: 5.699 ± 0.793
4.071GlyThr: 4.071 ± 0.614
6.839GlyVal: 6.839 ± 1.06
2.931GlyTrp: 2.931 ± 0.552
2.361GlyTyr: 2.361 ± 0.391
0.0GlyXaa: 0.0 ± 0.0
His
1.873HisAla: 1.873 ± 0.315
0.163HisCys: 0.163 ± 0.115
1.058HisAsp: 1.058 ± 0.293
0.651HisGlu: 0.651 ± 0.227
0.57HisPhe: 0.57 ± 0.206
1.384HisGly: 1.384 ± 0.311
0.163HisHis: 0.163 ± 0.124
0.244HisIle: 0.244 ± 0.142
0.651HisLys: 0.651 ± 0.217
1.303HisLeu: 1.303 ± 0.304
0.244HisMet: 0.244 ± 0.131
0.244HisAsn: 0.244 ± 0.124
1.221HisPro: 1.221 ± 0.331
0.733HisGln: 0.733 ± 0.254
1.14HisArg: 1.14 ± 0.34
0.488HisSer: 0.488 ± 0.187
0.896HisThr: 0.896 ± 0.238
1.628HisVal: 1.628 ± 0.38
0.326HisTrp: 0.326 ± 0.175
0.326HisTyr: 0.326 ± 0.136
0.0HisXaa: 0.0 ± 0.0
Ile
4.233IleAla: 4.233 ± 0.595
0.163IleCys: 0.163 ± 0.111
2.687IleAsp: 2.687 ± 0.475
2.442IleGlu: 2.442 ± 0.394
0.651IlePhe: 0.651 ± 0.222
3.419IleGly: 3.419 ± 0.506
0.651IleHis: 0.651 ± 0.226
1.384IleIle: 1.384 ± 0.375
1.628IleLys: 1.628 ± 0.322
3.419IleLeu: 3.419 ± 0.445
0.651IleMet: 0.651 ± 0.174
0.896IleAsn: 0.896 ± 0.238
1.384IlePro: 1.384 ± 0.388
1.058IleGln: 1.058 ± 0.258
3.582IleArg: 3.582 ± 0.59
2.28IleSer: 2.28 ± 0.535
2.442IleThr: 2.442 ± 0.426
2.605IleVal: 2.605 ± 0.449
0.488IleTrp: 0.488 ± 0.179
0.163IleTyr: 0.163 ± 0.096
0.0IleXaa: 0.0 ± 0.0
Lys
5.536LysAla: 5.536 ± 0.747
0.163LysCys: 0.163 ± 0.127
1.221LysAsp: 1.221 ± 0.397
1.628LysGlu: 1.628 ± 0.347
1.14LysPhe: 1.14 ± 0.313
2.361LysGly: 2.361 ± 0.505
0.977LysHis: 0.977 ± 0.26
1.14LysIle: 1.14 ± 0.333
2.035LysLys: 2.035 ± 0.42
2.117LysLeu: 2.117 ± 0.491
1.954LysMet: 1.954 ± 0.355
1.303LysAsn: 1.303 ± 0.309
2.117LysPro: 2.117 ± 0.553
1.465LysGln: 1.465 ± 0.367
2.687LysArg: 2.687 ± 0.512
2.035LysSer: 2.035 ± 0.364
2.442LysThr: 2.442 ± 0.482
2.687LysVal: 2.687 ± 0.379
0.407LysTrp: 0.407 ± 0.167
0.57LysTyr: 0.57 ± 0.222
0.0LysXaa: 0.0 ± 0.0
Leu
12.538LeuAla: 12.538 ± 0.989
1.14LeuCys: 1.14 ± 0.248
5.048LeuAsp: 5.048 ± 0.709
8.467LeuGlu: 8.467 ± 0.74
2.117LeuPhe: 2.117 ± 0.388
9.444LeuGly: 9.444 ± 0.855
0.651LeuHis: 0.651 ± 0.21
3.094LeuIle: 3.094 ± 0.491
2.849LeuLys: 2.849 ± 0.6
8.386LeuLeu: 8.386 ± 0.915
1.628LeuMet: 1.628 ± 0.329
2.524LeuAsn: 2.524 ± 0.488
5.292LeuPro: 5.292 ± 0.592
2.361LeuGln: 2.361 ± 0.327
6.676LeuArg: 6.676 ± 0.677
3.012LeuSer: 3.012 ± 0.563
5.292LeuThr: 5.292 ± 0.726
6.594LeuVal: 6.594 ± 0.672
1.628LeuTrp: 1.628 ± 0.36
1.465LeuTyr: 1.465 ± 0.361
0.0LeuXaa: 0.0 ± 0.0
Met
2.117MetAla: 2.117 ± 0.369
0.244MetCys: 0.244 ± 0.129
1.384MetAsp: 1.384 ± 0.4
0.977MetGlu: 0.977 ± 0.291
0.326MetPhe: 0.326 ± 0.164
2.28MetGly: 2.28 ± 0.639
0.0MetHis: 0.0 ± 0.0
0.733MetIle: 0.733 ± 0.25
0.896MetLys: 0.896 ± 0.265
1.465MetLeu: 1.465 ± 0.361
0.163MetMet: 0.163 ± 0.124
0.488MetAsn: 0.488 ± 0.209
0.977MetPro: 0.977 ± 0.266
0.244MetGln: 0.244 ± 0.145
2.28MetArg: 2.28 ± 0.365
2.198MetSer: 2.198 ± 0.489
1.628MetThr: 1.628 ± 0.303
1.628MetVal: 1.628 ± 0.407
0.326MetTrp: 0.326 ± 0.161
0.081MetTyr: 0.081 ± 0.076
0.0MetXaa: 0.0 ± 0.0
Asn
1.303AsnAla: 1.303 ± 0.238
0.0AsnCys: 0.0 ± 0.0
1.465AsnAsp: 1.465 ± 0.301
1.303AsnGlu: 1.303 ± 0.349
0.651AsnPhe: 0.651 ± 0.203
3.175AsnGly: 3.175 ± 0.522
0.244AsnHis: 0.244 ± 0.139
0.896AsnIle: 0.896 ± 0.251
1.465AsnLys: 1.465 ± 0.351
2.605AsnLeu: 2.605 ± 0.431
0.57AsnMet: 0.57 ± 0.194
0.488AsnAsn: 0.488 ± 0.249
1.71AsnPro: 1.71 ± 0.291
0.733AsnGln: 0.733 ± 0.207
1.628AsnArg: 1.628 ± 0.35
1.547AsnSer: 1.547 ± 0.344
1.303AsnThr: 1.303 ± 0.315
1.628AsnVal: 1.628 ± 0.434
0.488AsnTrp: 0.488 ± 0.198
0.488AsnTyr: 0.488 ± 0.228
0.0AsnXaa: 0.0 ± 0.0
Pro
8.141ProAla: 8.141 ± 0.975
0.488ProCys: 0.488 ± 0.202
3.745ProAsp: 3.745 ± 0.553
4.966ProGlu: 4.966 ± 0.803
1.058ProPhe: 1.058 ± 0.277
5.129ProGly: 5.129 ± 0.549
0.814ProHis: 0.814 ± 0.243
1.628ProIle: 1.628 ± 0.385
1.384ProLys: 1.384 ± 0.322
3.989ProLeu: 3.989 ± 0.64
0.814ProMet: 0.814 ± 0.235
0.896ProAsn: 0.896 ± 0.239
3.501ProPro: 3.501 ± 0.79
0.733ProGln: 0.733 ± 0.268
3.664ProArg: 3.664 ± 0.576
3.501ProSer: 3.501 ± 0.674
2.524ProThr: 2.524 ± 0.458
5.862ProVal: 5.862 ± 0.742
1.873ProTrp: 1.873 ± 0.29
0.651ProTyr: 0.651 ± 0.19
0.0ProXaa: 0.0 ± 0.0
Gln
3.501GlnAla: 3.501 ± 0.476
0.081GlnCys: 0.081 ± 0.081
0.814GlnAsp: 0.814 ± 0.262
1.628GlnGlu: 1.628 ± 0.371
0.733GlnPhe: 0.733 ± 0.256
2.361GlnGly: 2.361 ± 0.449
0.814GlnHis: 0.814 ± 0.219
1.547GlnIle: 1.547 ± 0.3
1.628GlnLys: 1.628 ± 0.313
3.501GlnLeu: 3.501 ± 0.644
0.488GlnMet: 0.488 ± 0.204
1.221GlnAsn: 1.221 ± 0.284
1.303GlnPro: 1.303 ± 0.335
0.57GlnGln: 0.57 ± 0.218
1.058GlnArg: 1.058 ± 0.297
0.733GlnSer: 0.733 ± 0.216
1.547GlnThr: 1.547 ± 0.416
2.198GlnVal: 2.198 ± 0.543
0.733GlnTrp: 0.733 ± 0.227
0.57GlnTyr: 0.57 ± 0.248
0.0GlnXaa: 0.0 ± 0.0
Arg
10.421ArgAla: 10.421 ± 0.977
1.384ArgCys: 1.384 ± 0.303
4.071ArgAsp: 4.071 ± 0.536
4.396ArgGlu: 4.396 ± 0.675
2.442ArgPhe: 2.442 ± 0.41
6.92ArgGly: 6.92 ± 0.839
0.896ArgHis: 0.896 ± 0.29
3.175ArgIle: 3.175 ± 0.48
3.175ArgLys: 3.175 ± 0.48
6.757ArgLeu: 6.757 ± 0.829
1.221ArgMet: 1.221 ± 0.289
1.791ArgAsn: 1.791 ± 0.413
3.826ArgPro: 3.826 ± 0.584
2.442ArgGln: 2.442 ± 0.348
6.187ArgArg: 6.187 ± 0.884
3.908ArgSer: 3.908 ± 0.486
5.943ArgThr: 5.943 ± 0.845
5.862ArgVal: 5.862 ± 0.718
1.14ArgTrp: 1.14 ± 0.339
1.628ArgTyr: 1.628 ± 0.355
0.0ArgXaa: 0.0 ± 0.0
Ser
6.676SerAla: 6.676 ± 0.815
0.244SerCys: 0.244 ± 0.136
2.198SerAsp: 2.198 ± 0.401
2.035SerGlu: 2.035 ± 0.321
1.384SerPhe: 1.384 ± 0.334
6.839SerGly: 6.839 ± 0.968
1.058SerHis: 1.058 ± 0.257
1.954SerIle: 1.954 ± 0.374
2.687SerLys: 2.687 ± 0.546
4.885SerLeu: 4.885 ± 0.611
1.14SerMet: 1.14 ± 0.268
0.977SerAsn: 0.977 ± 0.236
2.361SerPro: 2.361 ± 0.491
1.384SerGln: 1.384 ± 0.288
4.071SerArg: 4.071 ± 0.588
3.338SerSer: 3.338 ± 0.572
2.605SerThr: 2.605 ± 0.436
4.803SerVal: 4.803 ± 0.655
1.873SerTrp: 1.873 ± 0.336
0.814SerTyr: 0.814 ± 0.275
0.0SerXaa: 0.0 ± 0.0
Thr
8.548ThrAla: 8.548 ± 0.926
0.488ThrCys: 0.488 ± 0.192
3.419ThrAsp: 3.419 ± 0.482
2.687ThrGlu: 2.687 ± 0.584
1.465ThrPhe: 1.465 ± 0.28
5.129ThrGly: 5.129 ± 0.714
0.814ThrHis: 0.814 ± 0.255
2.605ThrIle: 2.605 ± 0.514
1.71ThrLys: 1.71 ± 0.382
3.826ThrLeu: 3.826 ± 0.565
0.814ThrMet: 0.814 ± 0.25
1.71ThrAsn: 1.71 ± 0.498
2.768ThrPro: 2.768 ± 0.458
0.814ThrGln: 0.814 ± 0.217
3.908ThrArg: 3.908 ± 0.572
2.442ThrSer: 2.442 ± 0.53
3.664ThrThr: 3.664 ± 0.526
5.455ThrVal: 5.455 ± 0.953
1.303ThrTrp: 1.303 ± 0.318
1.71ThrTyr: 1.71 ± 0.469
0.0ThrXaa: 0.0 ± 0.0
Val
9.363ValAla: 9.363 ± 0.823
1.058ValCys: 1.058 ± 0.34
3.664ValAsp: 3.664 ± 0.493
6.187ValGlu: 6.187 ± 0.686
2.605ValPhe: 2.605 ± 0.366
6.839ValGly: 6.839 ± 1.115
1.954ValHis: 1.954 ± 0.395
2.361ValIle: 2.361 ± 0.478
3.419ValLys: 3.419 ± 0.656
7.002ValLeu: 7.002 ± 0.904
1.221ValMet: 1.221 ± 0.316
1.384ValAsn: 1.384 ± 0.303
6.432ValPro: 6.432 ± 0.826
2.117ValGln: 2.117 ± 0.418
6.594ValArg: 6.594 ± 0.721
3.582ValSer: 3.582 ± 0.561
5.862ValThr: 5.862 ± 0.984
7.734ValVal: 7.734 ± 0.907
2.117ValTrp: 2.117 ± 0.426
1.954ValTyr: 1.954 ± 0.401
0.0ValXaa: 0.0 ± 0.0
Trp
2.442TrpAla: 2.442 ± 0.404
0.081TrpCys: 0.081 ± 0.085
1.628TrpAsp: 1.628 ± 0.325
0.977TrpGlu: 0.977 ± 0.286
0.57TrpPhe: 0.57 ± 0.214
1.547TrpGly: 1.547 ± 0.379
0.57TrpHis: 0.57 ± 0.219
0.896TrpIle: 0.896 ± 0.368
0.488TrpLys: 0.488 ± 0.192
1.954TrpLeu: 1.954 ± 0.387
0.326TrpMet: 0.326 ± 0.147
0.977TrpAsn: 0.977 ± 0.296
1.465TrpPro: 1.465 ± 0.346
0.814TrpGln: 0.814 ± 0.276
2.035TrpArg: 2.035 ± 0.407
1.71TrpSer: 1.71 ± 0.376
1.465TrpThr: 1.465 ± 0.343
2.361TrpVal: 2.361 ± 0.51
0.733TrpTrp: 0.733 ± 0.247
0.57TrpTyr: 0.57 ± 0.314
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.954TyrAla: 1.954 ± 0.408
0.407TyrCys: 0.407 ± 0.181
1.465TyrAsp: 1.465 ± 0.272
1.547TyrGlu: 1.547 ± 0.325
0.651TyrPhe: 0.651 ± 0.224
1.71TyrGly: 1.71 ± 0.426
0.244TyrHis: 0.244 ± 0.154
0.407TyrIle: 0.407 ± 0.151
0.651TyrLys: 0.651 ± 0.217
2.361TyrLeu: 2.361 ± 0.462
0.407TyrMet: 0.407 ± 0.173
0.57TyrAsn: 0.57 ± 0.266
0.651TyrPro: 0.651 ± 0.194
0.326TyrGln: 0.326 ± 0.192
1.873TyrArg: 1.873 ± 0.402
1.465TyrSer: 1.465 ± 0.315
0.733TyrThr: 0.733 ± 0.251
1.547TyrVal: 1.547 ± 0.315
0.163TyrTrp: 0.163 ± 0.109
0.326TyrTyr: 0.326 ± 0.157
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (12284 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski