Cherry associated luteovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Luteovirus

Average proteome isoelectric point is 7.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I9W765|A0A1I9W765_9LUTE RNA-directed RNA polymerase OS=Cherry associated luteovirus OX=1912598 PE=4 SV=1
MM1 pKa = 7.57LFDD4 pKa = 3.88TLLFASAKK12 pKa = 9.36LVKK15 pKa = 10.71DD16 pKa = 4.14FVSYY20 pKa = 10.47LYY22 pKa = 11.25ANLKK26 pKa = 9.79FVYY29 pKa = 9.91KK30 pKa = 10.29SLKK33 pKa = 9.55RR34 pKa = 11.84WLLEE38 pKa = 3.72LQGKK42 pKa = 8.25FRR44 pKa = 11.84QHH46 pKa = 6.93DD47 pKa = 3.87SFVDD51 pKa = 3.53MVYY54 pKa = 11.22GFMDD58 pKa = 3.76DD59 pKa = 4.39VEE61 pKa = 5.55DD62 pKa = 5.0FEE64 pKa = 4.84FDD66 pKa = 3.86CVVDD70 pKa = 4.95LEE72 pKa = 4.2AAEE75 pKa = 4.34LQFSKK80 pKa = 10.79AKK82 pKa = 10.63YY83 pKa = 9.17MLEE86 pKa = 3.9QMEE89 pKa = 4.6KK90 pKa = 10.49ARR92 pKa = 11.84GVLPEE97 pKa = 4.29WPQPMRR103 pKa = 11.84PLGADD108 pKa = 3.66PIGPPTLIDD117 pKa = 3.75GEE119 pKa = 4.71VIPDD123 pKa = 4.7PIVDD127 pKa = 3.35RR128 pKa = 11.84VKK130 pKa = 10.98FEE132 pKa = 3.91NDD134 pKa = 2.8QAHH137 pKa = 6.48VIVQNAFSEE146 pKa = 4.55AEE148 pKa = 3.92ILGQTSIFCHH158 pKa = 5.95AKK160 pKa = 9.71YY161 pKa = 10.76DD162 pKa = 4.61LIQAEE167 pKa = 3.95EE168 pKa = 4.4RR169 pKa = 11.84IRR171 pKa = 11.84DD172 pKa = 3.25KK173 pKa = 11.11YY174 pKa = 10.88ARR176 pKa = 11.84EE177 pKa = 3.88KK178 pKa = 10.56GEE180 pKa = 4.08HH181 pKa = 5.39YY182 pKa = 10.39FGRR185 pKa = 11.84FINTFSSRR193 pKa = 11.84LAYY196 pKa = 9.99VKK198 pKa = 10.25KK199 pKa = 10.1CAARR203 pKa = 11.84RR204 pKa = 11.84IEE206 pKa = 4.03SSRR209 pKa = 11.84LAEE212 pKa = 4.08KK213 pKa = 10.19VQKK216 pKa = 10.41QVFQVDD222 pKa = 3.56NVPDD226 pKa = 4.78FEE228 pKa = 5.24AMCDD232 pKa = 3.34MVQIEE237 pKa = 4.23TGEE240 pKa = 4.19TKK242 pKa = 9.81EE243 pKa = 4.18VKK245 pKa = 10.42GGEE248 pKa = 4.32DD249 pKa = 3.42GSEE252 pKa = 3.96PTTVPIKK259 pKa = 10.11KK260 pKa = 8.49WVRR263 pKa = 11.84RR264 pKa = 11.84LRR266 pKa = 11.84LDD268 pKa = 3.43DD269 pKa = 4.79AKK271 pKa = 11.19VKK273 pKa = 10.57KK274 pKa = 9.85DD275 pKa = 2.81ACKK278 pKa = 10.12FIRR281 pKa = 11.84RR282 pKa = 11.84YY283 pKa = 9.89VIHH286 pKa = 6.83HH287 pKa = 5.68NCRR290 pKa = 11.84LNSDD294 pKa = 4.02EE295 pKa = 4.32VSVMTINRR303 pKa = 11.84YY304 pKa = 8.03VAQFCDD310 pKa = 5.29LFKK313 pKa = 10.96LDD315 pKa = 4.54QNSTDD320 pKa = 3.95YY321 pKa = 11.59LMKK324 pKa = 10.53AALLMVPIVTKK335 pKa = 10.47EE336 pKa = 3.97DD337 pKa = 3.59LVSTMVIHH345 pKa = 6.98SPAARR350 pKa = 11.84DD351 pKa = 3.43LRR353 pKa = 11.84AMKK356 pKa = 9.38DD357 pKa = 3.43TVEE360 pKa = 4.28SGVFF364 pKa = 3.19

Molecular weight: 41.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I9W771|A0A1I9W771_9LUTE Putative P7 protein OS=Cherry associated luteovirus OX=1912598 PE=4 SV=1
MM1 pKa = 7.31NPGKK5 pKa = 9.77QVHH8 pKa = 5.99LVNSGVYY15 pKa = 9.58IGALIGGKK23 pKa = 9.89LGGSSTPPYY32 pKa = 10.1YY33 pKa = 10.42FWLVRR38 pKa = 11.84QTNSTKK44 pKa = 9.91IQKK47 pKa = 9.9QSVLLLSFMKK57 pKa = 10.25LRR59 pKa = 11.84SPIHH63 pKa = 6.18VGVKK67 pKa = 10.07NMGRR71 pKa = 11.84VEE73 pKa = 4.63LSQLCRR79 pKa = 3.49

Molecular weight: 8.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

2477

45

890

309.6

34.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.773 ± 0.421

1.494 ± 0.335

5.612 ± 0.79

5.491 ± 0.675

5.127 ± 0.661

6.742 ± 1.136

1.655 ± 0.256

5.45 ± 0.234

6.5 ± 0.618

7.751 ± 0.929

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.584 ± 0.495

4.037 ± 0.705

5.531 ± 0.625

4.522 ± 0.375

6.419 ± 0.311

8.236 ± 1.399

4.723 ± 0.636

7.792 ± 0.824

1.171 ± 0.136

3.149 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski