Amino acid dipepetide frequency for Methylocella silvestris (strain DSM 15510 / CIP 108128 / LMG 27833 / NCIMB 13906 / BL2)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.799AlaAla: 21.799 ± 0.232	1.193AlaCys: 1.193 ± 0.039	7.029AlaAsp: 7.029 ± 0.091	7.916AlaGlu: 7.916 ± 0.1	5.03AlaPhe: 5.03 ± 0.067	11.355AlaGly: 11.355 ± 0.108	2.398AlaHis: 2.398 ± 0.044	7.176AlaIle: 7.176 ± 0.082	4.79AlaLys: 4.79 ± 0.081	15.181AlaLeu: 15.181 ± 0.136	3.117AlaMet: 3.117 ± 0.053	3.21AlaAsn: 3.21 ± 0.059	7.91AlaPro: 7.91 ± 0.115	4.436AlaGln: 4.436 ± 0.065	9.825AlaArg: 9.825 ± 0.1	7.548AlaSer: 7.548 ± 0.086	6.02AlaThr: 6.02 ± 0.078	8.223AlaVal: 8.223 ± 0.088	1.404AlaTrp: 1.404 ± 0.04	2.65AlaTyr: 2.65 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	1.282CysAla: 1.282 ± 0.035	0.126CysCys: 0.126 ± 0.01	0.534CysAsp: 0.534 ± 0.021	0.453CysGlu: 0.453 ± 0.02	0.358CysPhe: 0.358 ± 0.016	1.023CysGly: 1.023 ± 0.033	0.212CysHis: 0.212 ± 0.017	0.356CysIle: 0.356 ± 0.018	0.222CysLys: 0.222 ± 0.012	0.867CysLeu: 0.867 ± 0.024	0.159CysMet: 0.159 ± 0.011	0.211CysAsn: 0.211 ± 0.013	0.472CysPro: 0.472 ± 0.022	0.215CysGln: 0.215 ± 0.013	0.614CysArg: 0.614 ± 0.024	0.483CysSer: 0.483 ± 0.021	0.317CysThr: 0.317 ± 0.017	0.715CysVal: 0.715 ± 0.026	0.098CysTrp: 0.098 ± 0.008	0.212CysTyr: 0.212 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	7.415AspAla: 7.415 ± 0.085	0.535AspCys: 0.535 ± 0.024	2.933AspAsp: 2.933 ± 0.055	3.422AspGlu: 3.422 ± 0.058	2.384AspPhe: 2.384 ± 0.043	4.84AspGly: 4.84 ± 0.069	1.217AspHis: 1.217 ± 0.033	2.841AspIle: 2.841 ± 0.053	1.817AspLys: 1.817 ± 0.036	6.086AspLeu: 6.086 ± 0.08	1.106AspMet: 1.106 ± 0.032	1.238AspAsn: 1.238 ± 0.035	3.618AspPro: 3.618 ± 0.056	1.647AspGln: 1.647 ± 0.042	3.948AspArg: 3.948 ± 0.065	2.332AspSer: 2.332 ± 0.049	1.76AspThr: 1.76 ± 0.042	3.905AspVal: 3.905 ± 0.062	0.882AspTrp: 0.882 ± 0.028	1.48AspTyr: 1.48 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	8.538GluAla: 8.538 ± 0.111	0.34GluCys: 0.34 ± 0.018	2.68GluAsp: 2.68 ± 0.049	2.944GluGlu: 2.944 ± 0.06	1.847GluPhe: 1.847 ± 0.041	4.059GluGly: 4.059 ± 0.069	1.038GluHis: 1.038 ± 0.033	3.702GluIle: 3.702 ± 0.057	2.363GluLys: 2.363 ± 0.052	5.311GluLeu: 5.311 ± 0.065	1.243GluMet: 1.243 ± 0.032	1.383GluAsn: 1.383 ± 0.029	2.916GluPro: 2.916 ± 0.057	1.886GluGln: 1.886 ± 0.038	4.821GluArg: 4.821 ± 0.073	2.632GluSer: 2.632 ± 0.049	3.466GluThr: 3.466 ± 0.055	3.228GluVal: 3.228 ± 0.061	0.634GluTrp: 0.634 ± 0.025	0.846GluTyr: 0.846 ± 0.028	0.0GluXaa: 0.0 ± 0.0
Phe	5.457PheAla: 5.457 ± 0.073	0.469PheCys: 0.469 ± 0.02	2.769PheAsp: 2.769 ± 0.045	2.443PheGlu: 2.443 ± 0.052	1.697PhePhe: 1.697 ± 0.046	3.935PheGly: 3.935 ± 0.062	0.762PheHis: 0.762 ± 0.028	1.87PheIle: 1.87 ± 0.041	1.182PheLys: 1.182 ± 0.038	3.678PheLeu: 3.678 ± 0.066	0.772PheMet: 0.772 ± 0.024	1.126PheAsn: 1.126 ± 0.032	1.744PhePro: 1.744 ± 0.041	0.969PheGln: 0.969 ± 0.029	2.31PheArg: 2.31 ± 0.043	2.596PheSer: 2.596 ± 0.047	1.875PheThr: 1.875 ± 0.039	2.969PheVal: 2.969 ± 0.048	0.561PheTrp: 0.561 ± 0.022	0.966PheTyr: 0.966 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	12.024GlyAla: 12.024 ± 0.127	0.9GlyCys: 0.9 ± 0.03	4.605GlyAsp: 4.605 ± 0.063	4.737GlyGlu: 4.737 ± 0.078	3.967GlyPhe: 3.967 ± 0.064	7.557GlyGly: 7.557 ± 0.119	1.663GlyHis: 1.663 ± 0.039	3.174GlyIle: 3.174 ± 0.058	3.28GlyLys: 3.28 ± 0.055	8.8GlyLeu: 8.8 ± 0.101	1.756GlyMet: 1.756 ± 0.034	1.794GlyAsn: 1.794 ± 0.049	3.825GlyPro: 3.825 ± 0.06	2.591GlyGln: 2.591 ± 0.058	6.11GlyArg: 6.11 ± 0.079	4.518GlySer: 4.518 ± 0.063	3.126GlyThr: 3.126 ± 0.064	6.996GlyVal: 6.996 ± 0.076	1.265GlyTrp: 1.265 ± 0.036	2.06GlyTyr: 2.06 ± 0.045	0.0GlyXaa: 0.0 ± 0.0
His	2.217HisAla: 2.217 ± 0.043	0.213HisCys: 0.213 ± 0.014	1.057HisAsp: 1.057 ± 0.028	1.014HisGlu: 1.014 ± 0.029	0.922HisPhe: 0.922 ± 0.031	1.901HisGly: 1.901 ± 0.038	0.467HisHis: 0.467 ± 0.022	0.999HisIle: 0.999 ± 0.029	0.519HisLys: 0.519 ± 0.021	1.862HisLeu: 1.862 ± 0.043	0.399HisMet: 0.399 ± 0.018	0.453HisAsn: 0.453 ± 0.02	1.24HisPro: 1.24 ± 0.031	0.501HisGln: 0.501 ± 0.021	1.222HisArg: 1.222 ± 0.031	1.006HisSer: 1.006 ± 0.029	0.698HisThr: 0.698 ± 0.024	1.381HisVal: 1.381 ± 0.035	0.298HisTrp: 0.298 ± 0.015	0.514HisTyr: 0.514 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	7.846IleAla: 7.846 ± 0.098	0.527IleCys: 0.527 ± 0.019	3.665IleAsp: 3.665 ± 0.055	3.849IleGlu: 3.849 ± 0.054	2.04IlePhe: 2.04 ± 0.046	5.161IleGly: 5.161 ± 0.065	0.907IleHis: 0.907 ± 0.03	2.438IleIle: 2.438 ± 0.046	1.577IleLys: 1.577 ± 0.038	4.736IleLeu: 4.736 ± 0.065	0.92IleMet: 0.92 ± 0.029	1.411IleAsn: 1.411 ± 0.037	2.266IlePro: 2.266 ± 0.042	1.134IleGln: 1.134 ± 0.033	2.896IleArg: 2.896 ± 0.042	2.914IleSer: 2.914 ± 0.05	2.171IleThr: 2.171 ± 0.039	4.576IleVal: 4.576 ± 0.071	0.575IleTrp: 0.575 ± 0.023	1.133IleTyr: 1.133 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	4.663LysAla: 4.663 ± 0.081	0.192LysCys: 0.192 ± 0.012	1.804LysAsp: 1.804 ± 0.038	1.643LysGlu: 1.643 ± 0.04	1.103LysPhe: 1.103 ± 0.029	2.663LysGly: 2.663 ± 0.048	0.558LysHis: 0.558 ± 0.021	2.354LysIle: 2.354 ± 0.041	1.328LysLys: 1.328 ± 0.039	3.601LysLeu: 3.601 ± 0.056	0.772LysMet: 0.772 ± 0.028	0.919LysAsn: 0.919 ± 0.029	2.176LysPro: 2.176 ± 0.05	1.069LysGln: 1.069 ± 0.03	2.366LysArg: 2.366 ± 0.054	2.147LysSer: 2.147 ± 0.052	2.13LysThr: 2.13 ± 0.042	2.207LysVal: 2.207 ± 0.046	0.354LysTrp: 0.354 ± 0.021	0.642LysTyr: 0.642 ± 0.027	0.0LysXaa: 0.0 ± 0.0
Leu	14.767LeuAla: 14.767 ± 0.15	0.993LeuCys: 0.993 ± 0.029	6.05LeuAsp: 6.05 ± 0.078	5.335LeuGlu: 5.335 ± 0.074	3.899LeuPhe: 3.899 ± 0.064	8.195LeuGly: 8.195 ± 0.104	1.771LeuHis: 1.771 ± 0.039	5.681LeuIle: 5.681 ± 0.068	3.81LeuLys: 3.81 ± 0.065	9.361LeuLeu: 9.361 ± 0.118	2.024LeuMet: 2.024 ± 0.048	2.642LeuAsn: 2.642 ± 0.05	5.503LeuPro: 5.503 ± 0.077	2.8LeuGln: 2.8 ± 0.059	7.326LeuArg: 7.326 ± 0.07	6.605LeuSer: 6.605 ± 0.085	5.445LeuThr: 5.445 ± 0.072	6.854LeuVal: 6.854 ± 0.079	1.108LeuTrp: 1.108 ± 0.034	2.14LeuTyr: 2.14 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	2.64MetAla: 2.64 ± 0.053	0.135MetCys: 0.135 ± 0.009	0.865MetAsp: 0.865 ± 0.027	0.889MetGlu: 0.889 ± 0.027	0.667MetPhe: 0.667 ± 0.024	1.437MetGly: 1.437 ± 0.033	0.351MetHis: 0.351 ± 0.016	1.294MetIle: 1.294 ± 0.032	0.895MetLys: 0.895 ± 0.026	2.107MetLeu: 2.107 ± 0.043	0.522MetMet: 0.522 ± 0.025	0.693MetAsn: 0.693 ± 0.023	1.208MetPro: 1.208 ± 0.033	0.748MetGln: 0.748 ± 0.026	1.739MetArg: 1.739 ± 0.04	1.562MetSer: 1.562 ± 0.036	1.627MetThr: 1.627 ± 0.037	1.169MetVal: 1.169 ± 0.029	0.165MetTrp: 0.165 ± 0.011	0.257MetTyr: 0.257 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	3.258AsnAla: 3.258 ± 0.052	0.283AsnCys: 0.283 ± 0.017	1.292AsnAsp: 1.292 ± 0.037	1.277AsnGlu: 1.277 ± 0.035	1.123AsnPhe: 1.123 ± 0.031	2.274AsnGly: 2.274 ± 0.051	0.515AsnHis: 0.515 ± 0.021	1.412AsnIle: 1.412 ± 0.04	0.709AsnLys: 0.709 ± 0.028	2.622AsnLeu: 2.622 ± 0.051	0.522AsnMet: 0.522 ± 0.019	0.695AsnAsn: 0.695 ± 0.028	1.811AsnPro: 1.811 ± 0.046	0.715AsnGln: 0.715 ± 0.027	1.557AsnArg: 1.557 ± 0.038	1.357AsnSer: 1.357 ± 0.036	1.059AsnThr: 1.059 ± 0.035	1.9AsnVal: 1.9 ± 0.047	0.422AsnTrp: 0.422 ± 0.017	0.683AsnTyr: 0.683 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	7.407ProAla: 7.407 ± 0.107	0.362ProCys: 0.362 ± 0.019	3.552ProAsp: 3.552 ± 0.055	3.333ProGlu: 3.333 ± 0.061	2.248ProPhe: 2.248 ± 0.041	4.82ProGly: 4.82 ± 0.068	1.103ProHis: 1.103 ± 0.03	2.572ProIle: 2.572 ± 0.049	1.899ProLys: 1.899 ± 0.041	5.159ProLeu: 5.159 ± 0.077	1.088ProMet: 1.088 ± 0.028	1.411ProAsn: 1.411 ± 0.034	3.477ProPro: 3.477 ± 0.084	2.021ProGln: 2.021 ± 0.044	3.338ProArg: 3.338 ± 0.059	3.044ProSer: 3.044 ± 0.06	2.409ProThr: 2.409 ± 0.045	3.701ProVal: 3.701 ± 0.055	0.688ProTrp: 0.688 ± 0.025	1.181ProTyr: 1.181 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	3.841GlnAla: 3.841 ± 0.056	0.212GlnCys: 0.212 ± 0.014	1.457GlnAsp: 1.457 ± 0.036	1.374GlnGlu: 1.374 ± 0.04	1.163GlnPhe: 1.163 ± 0.031	2.367GlnGly: 2.367 ± 0.043	0.559GlnHis: 0.559 ± 0.022	1.966GlnIle: 1.966 ± 0.049	1.281GlnLys: 1.281 ± 0.033	2.749GlnLeu: 2.749 ± 0.047	0.754GlnMet: 0.754 ± 0.026	0.921GlnAsn: 0.921 ± 0.026	1.718GlnPro: 1.718 ± 0.049	1.091GlnGln: 1.091 ± 0.042	2.333GlnArg: 2.333 ± 0.042	1.991GlnSer: 1.991 ± 0.044	1.775GlnThr: 1.775 ± 0.042	1.628GlnVal: 1.628 ± 0.037	0.359GlnTrp: 0.359 ± 0.018	0.597GlnTyr: 0.597 ± 0.023	0.0GlnXaa: 0.0 ± 0.0
Arg	8.999ArgAla: 8.999 ± 0.096	0.496ArgCys: 0.496 ± 0.022	3.937ArgAsp: 3.937 ± 0.054	4.113ArgGlu: 4.113 ± 0.072	3.085ArgPhe: 3.085 ± 0.051	4.957ArgGly: 4.957 ± 0.06	1.442ArgHis: 1.442 ± 0.038	3.877ArgIle: 3.877 ± 0.062	2.383ArgLys: 2.383 ± 0.043	8.38ArgLeu: 8.38 ± 0.103	1.566ArgMet: 1.566 ± 0.032	1.772ArgAsn: 1.772 ± 0.041	3.874ArgPro: 3.874 ± 0.061	2.437ArgGln: 2.437 ± 0.048	6.192ArgArg: 6.192 ± 0.086	3.757ArgSer: 3.757 ± 0.056	2.85ArgThr: 2.85 ± 0.043	4.302ArgVal: 4.302 ± 0.059	0.884ArgTrp: 0.884 ± 0.025	1.594ArgTyr: 1.594 ± 0.041	0.0ArgXaa: 0.0 ± 0.0
Ser	7.244SerAla: 7.244 ± 0.08	0.52SerCys: 0.52 ± 0.022	2.983SerAsp: 2.983 ± 0.061	2.875SerGlu: 2.875 ± 0.053	2.673SerPhe: 2.673 ± 0.053	5.624SerGly: 5.624 ± 0.074	1.107SerHis: 1.107 ± 0.029	2.995SerIle: 2.995 ± 0.054	1.643SerLys: 1.643 ± 0.035	5.934SerLeu: 5.934 ± 0.068	1.177SerMet: 1.177 ± 0.032	1.451SerAsn: 1.451 ± 0.038	3.142SerPro: 3.142 ± 0.055	1.621SerGln: 1.621 ± 0.037	3.749SerArg: 3.749 ± 0.058	3.165SerSer: 3.165 ± 0.063	2.471SerThr: 2.471 ± 0.05	3.918SerVal: 3.918 ± 0.055	0.729SerTrp: 0.729 ± 0.023	1.34SerTyr: 1.34 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	5.657ThrAla: 5.657 ± 0.082	0.376ThrCys: 0.376 ± 0.02	2.255ThrAsp: 2.255 ± 0.048	2.211ThrGlu: 2.211 ± 0.043	1.777ThrPhe: 1.777 ± 0.035	4.398ThrGly: 4.398 ± 0.061	0.927ThrHis: 0.927 ± 0.031	2.814ThrIle: 2.814 ± 0.055	1.482ThrLys: 1.482 ± 0.037	5.558ThrLeu: 5.558 ± 0.075	0.914ThrMet: 0.914 ± 0.029	1.176ThrAsn: 1.176 ± 0.038	3.363ThrPro: 3.363 ± 0.055	1.447ThrGln: 1.447 ± 0.038	3.041ThrArg: 3.041 ± 0.055	2.488ThrSer: 2.488 ± 0.049	2.456ThrThr: 2.456 ± 0.055	3.32ThrVal: 3.32 ± 0.053	0.462ThrTrp: 0.462 ± 0.019	1.036ThrTyr: 1.036 ± 0.03	0.0ThrXaa: 0.0 ± 0.0
Val	9.37ValAla: 9.37 ± 0.093	0.696ValCys: 0.696 ± 0.026	4.076ValAsp: 4.076 ± 0.07	4.441ValGlu: 4.441 ± 0.06	2.694ValPhe: 2.694 ± 0.054	5.293ValGly: 5.293 ± 0.071	1.185ValHis: 1.185 ± 0.031	3.673ValIle: 3.673 ± 0.069	2.466ValLys: 2.466 ± 0.044	6.595ValLeu: 6.595 ± 0.081	1.497ValMet: 1.497 ± 0.036	1.987ValAsn: 1.987 ± 0.048	2.779ValPro: 2.779 ± 0.047	1.721ValGln: 1.721 ± 0.039	4.42ValArg: 4.42 ± 0.072	4.164ValSer: 4.164 ± 0.069	3.833ValThr: 3.833 ± 0.06	5.091ValVal: 5.091 ± 0.078	0.837ValTrp: 0.837 ± 0.031	1.505ValTyr: 1.505 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	1.153TrpAla: 1.153 ± 0.037	0.106TrpCys: 0.106 ± 0.01	0.531TrpAsp: 0.531 ± 0.023	0.433TrpGlu: 0.433 ± 0.019	0.503TrpPhe: 0.503 ± 0.02	0.843TrpGly: 0.843 ± 0.028	0.261TrpHis: 0.261 ± 0.015	0.673TrpIle: 0.673 ± 0.026	0.399TrpLys: 0.399 ± 0.02	1.573TrpLeu: 1.573 ± 0.036	0.271TrpMet: 0.271 ± 0.014	0.375TrpAsn: 0.375 ± 0.019	0.69TrpPro: 0.69 ± 0.021	0.376TrpGln: 0.376 ± 0.018	1.38TrpArg: 1.38 ± 0.036	0.859TrpSer: 0.859 ± 0.028	0.77TrpThr: 0.77 ± 0.029	0.596TrpVal: 0.596 ± 0.024	0.182TrpTrp: 0.182 ± 0.012	0.248TrpTyr: 0.248 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.636TyrAla: 2.636 ± 0.045	0.254TyrCys: 0.254 ± 0.014	1.33TyrAsp: 1.33 ± 0.035	1.176TyrGlu: 1.176 ± 0.031	0.969TyrPhe: 0.969 ± 0.031	2.044TyrGly: 2.044 ± 0.05	0.441TyrHis: 0.441 ± 0.02	0.875TyrIle: 0.875 ± 0.03	0.647TyrLys: 0.647 ± 0.025	2.152TyrLeu: 2.152 ± 0.046	0.387TyrMet: 0.387 ± 0.018	0.617TyrAsn: 0.617 ± 0.029	1.08TyrPro: 1.08 ± 0.036	0.711TyrGln: 0.711 ± 0.028	1.711TyrArg: 1.711 ± 0.04	1.193TyrSer: 1.193 ± 0.034	0.9TyrThr: 0.9 ± 0.029	1.647TyrVal: 1.647 ± 0.037	0.315TyrTrp: 0.315 ± 0.017	0.602TyrTyr: 0.602 ± 0.028	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3816 proteins (1219436 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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