Phlebiopsis gigantea 11061_1 CR5-6

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetes incertae sedis; Polyporales; Phanerochaetaceae; Phlebiopsis; Phlebiopsis gigantea

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11848 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C3PGL3|A0A0C3PGL3_PHLGI Uncharacterized protein OS=Phlebiopsis gigantea 11061_1 CR5-6 OX=745531 GN=PHLGIDRAFT_129223 PE=4 SV=1
MM1 pKa = 6.82STEE4 pKa = 3.99PQSLFSLARR13 pKa = 11.84NKK15 pKa = 10.16LQSVVGGGVKK25 pKa = 10.32DD26 pKa = 3.46SSSLHH31 pKa = 5.33RR32 pKa = 11.84WVLLKK37 pKa = 10.98NSIIRR42 pKa = 11.84SHH44 pKa = 5.87VQTPATSQALDD55 pKa = 3.46EE56 pKa = 4.96AEE58 pKa = 4.12ADD60 pKa = 3.46SVYY63 pKa = 10.83RR64 pKa = 11.84QDD66 pKa = 3.4EE67 pKa = 4.32EE68 pKa = 4.55IYY70 pKa = 10.47EE71 pKa = 4.21VEE73 pKa = 4.02HH74 pKa = 6.86DD75 pKa = 4.23AFMFPDD81 pKa = 3.83PHH83 pKa = 6.44SACADD88 pKa = 3.26SSAAGNDD95 pKa = 3.21EE96 pKa = 4.09NQWLDD101 pKa = 3.45SVLEE105 pKa = 3.99GLGDD109 pKa = 4.47DD110 pKa = 4.12EE111 pKa = 7.28DD112 pKa = 6.37DD113 pKa = 3.97YY114 pKa = 11.62FQAGALPFADD124 pKa = 5.5DD125 pKa = 5.27DD126 pKa = 4.38IEE128 pKa = 4.53PTSPMYY134 pKa = 11.12SPMSSSDD141 pKa = 3.89DD142 pKa = 3.68LAEE145 pKa = 3.97QPSYY149 pKa = 11.35YY150 pKa = 9.83DD151 pKa = 3.33YY152 pKa = 11.37PLIYY156 pKa = 10.11PPFRR160 pKa = 11.84PPVPSTWSPLEE171 pKa = 4.15HH172 pKa = 6.89SSDD175 pKa = 4.06SILPSTSALYY185 pKa = 10.4DD186 pKa = 3.4NPLPYY191 pKa = 10.41LDD193 pKa = 4.85VDD195 pKa = 4.26EE196 pKa = 6.38LDD198 pKa = 4.65DD199 pKa = 5.25SPVPDD204 pKa = 5.51AIEE207 pKa = 4.38DD208 pKa = 3.94TSDD211 pKa = 4.1DD212 pKa = 3.89EE213 pKa = 5.66SDD215 pKa = 3.84APSTPSAVSTPSLSPPGPTIPMPRR239 pKa = 11.84EE240 pKa = 3.57RR241 pKa = 11.84TRR243 pKa = 11.84LLTHH247 pKa = 5.69PHH249 pKa = 5.99VYY251 pKa = 10.05VEE253 pKa = 4.13RR254 pKa = 11.84DD255 pKa = 3.18DD256 pKa = 4.77PYY258 pKa = 10.99FYY260 pKa = 10.53PFEE263 pKa = 6.33LDD265 pKa = 4.54LDD267 pKa = 4.32PLPLPDD273 pKa = 4.38DD274 pKa = 3.75TLAPSLRR281 pKa = 11.84AYY283 pKa = 10.37RR284 pKa = 11.84DD285 pKa = 3.7AYY287 pKa = 11.11SSEE290 pKa = 4.32CC291 pKa = 3.96

Molecular weight: 32.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C3PNC4|A0A0C3PNC4_PHLGI Uncharacterized protein OS=Phlebiopsis gigantea 11061_1 CR5-6 OX=745531 GN=PHLGIDRAFT_104544 PE=3 SV=1
VV1 pKa = 7.11ASCGSHH7 pKa = 4.89RR8 pKa = 11.84TSDD11 pKa = 3.7RR12 pKa = 11.84LCGGGEE18 pKa = 3.75PSVRR22 pKa = 11.84PRR24 pKa = 11.84VLGRR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84PRR32 pKa = 11.84HH33 pKa = 5.31PPGLRR38 pKa = 11.84PRR40 pKa = 11.84LPRR43 pKa = 11.84LAPALPPPSLQSRR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84AVRR61 pKa = 11.84PRR63 pKa = 11.84RR64 pKa = 11.84CLHH67 pKa = 5.84RR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84PARR73 pKa = 11.84PAPPPRR79 pKa = 11.84VPGPVRR85 pKa = 11.84PRR87 pKa = 11.84PRR89 pKa = 11.84RR90 pKa = 11.84SLRR93 pKa = 11.84PARR96 pKa = 11.84GPQAAPRR103 pKa = 11.84PDD105 pKa = 3.16AAARR109 pKa = 11.84RR110 pKa = 11.84AARR113 pKa = 11.84PAPSAALRR121 pKa = 11.84RR122 pKa = 11.84RR123 pKa = 11.84PLPAAPARR131 pKa = 11.84PGRR134 pKa = 11.84AHH136 pKa = 6.77AALRR140 pKa = 11.84PRR142 pKa = 11.84RR143 pKa = 11.84RR144 pKa = 11.84ALPRR148 pKa = 11.84QPRR151 pKa = 11.84LLLSARR157 pKa = 11.84LPPHH161 pKa = 6.86RR162 pKa = 11.84RR163 pKa = 11.84RR164 pKa = 11.84PPQPQAARR172 pKa = 11.84RR173 pKa = 11.84EE174 pKa = 4.19LARR177 pKa = 11.84LGHH180 pKa = 6.17RR181 pKa = 11.84PARR184 pKa = 11.84SSAARR189 pKa = 11.84AVPQVRR195 pKa = 11.84RR196 pKa = 11.84PPRR199 pKa = 11.84APLAGRR205 pKa = 11.84DD206 pKa = 3.46GALDD210 pKa = 3.62DD211 pKa = 4.5RR212 pKa = 11.84QPLGLPRR219 pKa = 11.84QLARR223 pKa = 11.84TLWHH227 pKa = 6.46RR228 pKa = 11.84RR229 pKa = 11.84RR230 pKa = 11.84SRR232 pKa = 11.84GARR235 pKa = 11.84PAPHH239 pKa = 6.18ARR241 pKa = 11.84ARR243 pKa = 11.84RR244 pKa = 11.84RR245 pKa = 11.84DD246 pKa = 3.05ARR248 pKa = 11.84RR249 pKa = 11.84RR250 pKa = 11.84RR251 pKa = 11.84RR252 pKa = 11.84PARR255 pKa = 11.84HH256 pKa = 5.69GKK258 pKa = 9.21AAPARR263 pKa = 11.84SRR265 pKa = 11.84RR266 pKa = 11.84RR267 pKa = 11.84TRR269 pKa = 11.84PGRR272 pKa = 11.84RR273 pKa = 11.84PRR275 pKa = 11.84RR276 pKa = 11.84AVRR279 pKa = 11.84AAARR283 pKa = 11.84IAGPHH288 pKa = 6.65APRR291 pKa = 11.84ALPPAHH297 pKa = 5.78TRR299 pKa = 11.84VLRR302 pKa = 11.84RR303 pKa = 11.84APRR306 pKa = 11.84RR307 pKa = 11.84PPLGPLGAPRR317 pKa = 11.84PALPRR322 pKa = 11.84ARR324 pKa = 11.84HH325 pKa = 5.26PPRR328 pKa = 11.84RR329 pKa = 11.84PVPAALRR336 pKa = 11.84AAALPALPDD345 pKa = 3.37GRR347 pKa = 11.84RR348 pKa = 11.84LARR351 pKa = 11.84ARR353 pKa = 11.84RR354 pKa = 11.84RR355 pKa = 11.84AAVRR359 pKa = 11.84RR360 pKa = 11.84AQAARR365 pKa = 11.84KK366 pKa = 8.86GRR368 pKa = 11.84LRR370 pKa = 11.84RR371 pKa = 11.84VRR373 pKa = 11.84PRR375 pKa = 11.84DD376 pKa = 3.2ARR378 pKa = 11.84RR379 pKa = 11.84AARR382 pKa = 11.84AGLGRR387 pKa = 11.84RR388 pKa = 11.84RR389 pKa = 11.84RR390 pKa = 11.84GLARR394 pKa = 11.84GVGRR398 pKa = 11.84GGARR402 pKa = 11.84GAAWDD407 pKa = 3.64ARR409 pKa = 11.84QGRR412 pKa = 11.84GARR415 pKa = 11.84VEE417 pKa = 3.78RR418 pKa = 11.84GGRR421 pKa = 11.84GRR423 pKa = 11.84PRR425 pKa = 11.84FVSRR429 pKa = 11.84PPTRR433 pKa = 11.84RR434 pKa = 11.84NTCAFQSLLRR444 pKa = 11.84AHH446 pKa = 6.98LEE448 pKa = 4.02VFQGISAHH456 pKa = 5.17VRR458 pKa = 11.84VGYY461 pKa = 10.42

Molecular weight: 51.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11848

0

11848

4868893

36

4819

410.9

45.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.365 ± 0.02

1.296 ± 0.01

5.544 ± 0.018

5.749 ± 0.027

3.649 ± 0.015

6.532 ± 0.023

2.604 ± 0.01

4.525 ± 0.017

4.195 ± 0.021

9.286 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.051 ± 0.008

3.098 ± 0.011

6.604 ± 0.029

3.691 ± 0.015

6.495 ± 0.025

8.388 ± 0.027

6.111 ± 0.014

6.659 ± 0.016

1.47 ± 0.009

2.681 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski