Tistrella mobilis (strain KA081020-065)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Tistrella; Tistrella mobilis

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5778 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I3TV30|I3TV30_TISMK Copper resistance protein CopC OS=Tistrella mobilis (strain KA081020-065) OX=1110502 GN=copC PE=3 SV=1
MM1 pKa = 8.05ADD3 pKa = 3.26TPYY6 pKa = 10.56ATLTPPSGWSVYY18 pKa = 10.5SNQGAEE24 pKa = 4.35SGLKK28 pKa = 7.45TTVSLSSGEE37 pKa = 4.1IVGGDD42 pKa = 3.59PAFLDD47 pKa = 4.33SIADD51 pKa = 3.45AFYY54 pKa = 10.78EE55 pKa = 4.35VALTAVEE62 pKa = 4.93AYY64 pKa = 7.2ITPSNIEE71 pKa = 4.66DD72 pKa = 4.11PLQNWGDD79 pKa = 3.77FLTSLKK85 pKa = 10.69SNTVIRR91 pKa = 11.84DD92 pKa = 3.61ALDD95 pKa = 3.85SIANVTAVQAVQAVAGNTVQTSGSALGEE123 pKa = 4.18LQDD126 pKa = 4.46EE127 pKa = 4.65TLQDD131 pKa = 3.19ATDD134 pKa = 4.76AIFSEE139 pKa = 4.61LNSGNVEE146 pKa = 4.1PGSLLGWYY154 pKa = 9.54QYY156 pKa = 10.25TIHH159 pKa = 7.41TITQLLTSTTLQFQKK174 pKa = 10.79SVVGNAVEE182 pKa = 4.64IYY184 pKa = 10.55LGLDD188 pKa = 3.31GQMVAASNSSSITYY202 pKa = 9.97SGNNSVVVQGGAADD216 pKa = 4.49DD217 pKa = 4.57LLTSGSRR224 pKa = 11.84DD225 pKa = 3.43DD226 pKa = 5.52VLVGWAGNDD235 pKa = 2.77IMIASEE241 pKa = 4.58GKK243 pKa = 8.4DD244 pKa = 3.27TYY246 pKa = 10.96IGGDD250 pKa = 3.3GTDD253 pKa = 3.2TVDD256 pKa = 2.95YY257 pKa = 11.29SGFHH261 pKa = 6.73GFATPLKK268 pKa = 10.37ASLATGKK275 pKa = 8.78GTSGPATGHH284 pKa = 5.98SYY286 pKa = 11.25VGVEE290 pKa = 4.0VLIGTAGGDD299 pKa = 3.47DD300 pKa = 3.79LTANGAGTTLYY311 pKa = 10.91GGGGNDD317 pKa = 3.53VLRR320 pKa = 11.84DD321 pKa = 3.57TSTTGSATLNGGDD334 pKa = 4.13GNDD337 pKa = 3.34TASYY341 pKa = 10.16FGRR344 pKa = 11.84TQGITASLSAPGDD357 pKa = 3.49TTGYY361 pKa = 10.22LISIEE366 pKa = 4.03NLYY369 pKa = 8.57GTDD372 pKa = 3.71YY373 pKa = 11.69NDD375 pKa = 3.31VLVGNSEE382 pKa = 4.06NNILTGLEE390 pKa = 4.26GNDD393 pKa = 3.42TLVGRR398 pKa = 11.84GGADD402 pKa = 3.07TLDD405 pKa = 3.54GRR407 pKa = 11.84GGSDD411 pKa = 2.9TASYY415 pKa = 10.82QSASSSVTADD425 pKa = 3.13LRR427 pKa = 11.84AGNGTQGDD435 pKa = 3.97AAGDD439 pKa = 3.64TLISIEE445 pKa = 4.26NLTGSAYY452 pKa = 10.39GDD454 pKa = 3.62KK455 pKa = 10.89LYY457 pKa = 11.12GDD459 pKa = 3.9SAANVLRR466 pKa = 11.84GGNGDD471 pKa = 3.43DD472 pKa = 3.63ALRR475 pKa = 11.84GRR477 pKa = 11.84GGADD481 pKa = 2.94TLDD484 pKa = 3.93GGNGFDD490 pKa = 3.48FASYY494 pKa = 11.13SDD496 pKa = 3.39ATASVTVDD504 pKa = 3.77LASGKK509 pKa = 10.21GLAGEE514 pKa = 4.31ALGDD518 pKa = 3.64TLVSIEE524 pKa = 4.01GLAGSGYY531 pKa = 10.8DD532 pKa = 5.63DD533 pKa = 5.58LLQGDD538 pKa = 4.15GNDD541 pKa = 3.05NYY543 pKa = 10.88FYY545 pKa = 11.44GNGGNDD551 pKa = 3.14ILRR554 pKa = 11.84GRR556 pKa = 11.84GGADD560 pKa = 2.91TFDD563 pKa = 4.2GGSGLDD569 pKa = 3.36FATYY573 pKa = 10.56SDD575 pKa = 3.85ASASVVADD583 pKa = 4.35LASGGISGEE592 pKa = 4.18AEE594 pKa = 3.54GDD596 pKa = 3.73VYY598 pKa = 11.07ISIEE602 pKa = 4.03GLTGTSYY609 pKa = 12.08ADD611 pKa = 3.77TLHH614 pKa = 7.07GDD616 pKa = 3.33AGSNTLVGRR625 pKa = 11.84DD626 pKa = 3.67GDD628 pKa = 4.62DD629 pKa = 2.99ILNGRR634 pKa = 11.84RR635 pKa = 11.84GADD638 pKa = 3.22QLIGGDD644 pKa = 3.54GFDD647 pKa = 3.26FATYY651 pKa = 10.31SEE653 pKa = 4.45ASAAVAVDD661 pKa = 4.22LASQRR666 pKa = 11.84GTAGEE671 pKa = 4.17AKK673 pKa = 10.36DD674 pKa = 5.44DD675 pKa = 3.63ILTEE679 pKa = 3.94IEE681 pKa = 4.13GLIGSAYY688 pKa = 10.47GDD690 pKa = 3.37TLSGNKK696 pKa = 8.69EE697 pKa = 4.01VNHH700 pKa = 6.25LRR702 pKa = 11.84GEE704 pKa = 4.28AGNDD708 pKa = 3.3VLDD711 pKa = 4.15GRR713 pKa = 11.84GGADD717 pKa = 3.79LLQGGAGADD726 pKa = 3.47QLTGGTGADD735 pKa = 2.96NFLYY739 pKa = 10.97LNLSDD744 pKa = 3.96SGKK747 pKa = 9.0TSGTWDD753 pKa = 3.82TITDD757 pKa = 4.35FSQTDD762 pKa = 3.28GDD764 pKa = 4.58KK765 pKa = 10.62IDD767 pKa = 3.72VSAIDD772 pKa = 4.63AYY774 pKa = 11.18VGSPGNQTFTFIGSAGYY791 pKa = 10.3SGTPGEE797 pKa = 4.24LRR799 pKa = 11.84ANVSNGYY806 pKa = 7.79TYY808 pKa = 10.55IGVDD812 pKa = 3.13VDD814 pKa = 4.06GDD816 pKa = 3.72QASDD820 pKa = 3.24MVIRR824 pKa = 11.84LNGIHH829 pKa = 6.86TLTAADD835 pKa = 5.33FILL838 pKa = 5.1

Molecular weight: 84.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I3TPA1|I3TPA1_TISMK Uncharacterized protein OS=Tistrella mobilis (strain KA081020-065) OX=1110502 GN=TMO_2751 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 8.95RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.63GFRR19 pKa = 11.84SRR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.06VLANRR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.9RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5778

0

5778

1925682

37

6469

333.3

35.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.444 ± 0.066

0.725 ± 0.01

6.164 ± 0.034

5.191 ± 0.027

3.245 ± 0.021

9.318 ± 0.033

2.005 ± 0.016

4.826 ± 0.02

2.031 ± 0.028

10.57 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.488 ± 0.017

1.871 ± 0.016

5.744 ± 0.031

2.63 ± 0.019

8.17 ± 0.04

4.295 ± 0.023

5.391 ± 0.032

7.629 ± 0.033

1.344 ± 0.014

1.919 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski