Bisgaard taxon 44 str. B96_4

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Psittacicellaceae; Psittacicella

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1522 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A1Y3C0|A0A3A1Y3C0_9PAST Uncharacterized protein OS=Bisgaard taxon 44 str. B96_4 OX=2028576 GN=CJP74_03430 PE=4 SV=1
MM1 pKa = 7.39SLIGIFYY8 pKa = 10.71GSDD11 pKa = 2.83TGNTEE16 pKa = 4.44NIAKK20 pKa = 9.05MLAEE24 pKa = 4.85KK25 pKa = 10.18IGPDD29 pKa = 3.37LCDD32 pKa = 3.24VYY34 pKa = 11.26DD35 pKa = 3.55IAKK38 pKa = 8.04CTKK41 pKa = 10.27EE42 pKa = 4.27DD43 pKa = 3.68LEE45 pKa = 4.5NYY47 pKa = 9.63NVLLLGIPTWYY58 pKa = 10.36YY59 pKa = 11.6GEE61 pKa = 4.91AQADD65 pKa = 3.72WEE67 pKa = 4.85DD68 pKa = 4.32FFPTLQAIDD77 pKa = 3.88FNDD80 pKa = 3.73RR81 pKa = 11.84VCAIFGCGDD90 pKa = 3.22QEE92 pKa = 5.04DD93 pKa = 4.44YY94 pKa = 11.9ADD96 pKa = 4.07YY97 pKa = 10.88FCDD100 pKa = 4.67AMGEE104 pKa = 4.15VANIVEE110 pKa = 4.41ANGAVVIGHH119 pKa = 6.41TSHH122 pKa = 7.71DD123 pKa = 3.92GYY125 pKa = 9.74TFTEE129 pKa = 4.49SKK131 pKa = 11.16ALIDD135 pKa = 3.98SNTLVGLCIDD145 pKa = 4.32EE146 pKa = 4.82DD147 pKa = 3.9RR148 pKa = 11.84QPEE151 pKa = 4.24LTEE154 pKa = 3.67EE155 pKa = 4.22RR156 pKa = 11.84VNNWVAQLKK165 pKa = 10.31NDD167 pKa = 4.22LGII170 pKa = 4.34

Molecular weight: 18.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A1Y772|A0A3A1Y772_9PAST Uncharacterized protein OS=Bisgaard taxon 44 str. B96_4 OX=2028576 GN=CJP74_02555 PE=3 SV=1
MM1 pKa = 7.32ARR3 pKa = 11.84FVRR6 pKa = 11.84RR7 pKa = 11.84RR8 pKa = 11.84KK9 pKa = 8.69MVRR12 pKa = 11.84QPEE15 pKa = 4.47LVTEE19 pKa = 4.27VDD21 pKa = 3.96YY22 pKa = 11.75KK23 pKa = 11.37DD24 pKa = 3.42VATLKK29 pKa = 10.83NFVNEE34 pKa = 3.98SGKK37 pKa = 10.07IVASRR42 pKa = 11.84LTGVDD47 pKa = 3.11AKK49 pKa = 8.29TQRR52 pKa = 11.84QLARR56 pKa = 11.84AIKK59 pKa = 9.21RR60 pKa = 11.84ARR62 pKa = 11.84YY63 pKa = 8.35LALIPYY69 pKa = 8.62TDD71 pKa = 3.45NQQ73 pKa = 3.46

Molecular weight: 8.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1522

0

1522

633264

28

10156

416.1

46.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.408 ± 0.135

0.792 ± 0.02

5.009 ± 0.057

5.789 ± 0.11

4.434 ± 0.115

5.931 ± 0.09

1.628 ± 0.053

6.274 ± 0.073

5.919 ± 0.092

10.139 ± 0.141

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.623 ± 0.06

6.784 ± 0.27

3.419 ± 0.102

4.859 ± 0.119

3.643 ± 0.093

6.859 ± 0.13

6.593 ± 0.283

7.289 ± 0.123

0.853 ± 0.037

3.757 ± 0.103
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski