Caulobacter phage Jess A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Rauchvirus; unclassified Rauchvirus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5NY06|A0A4Y5NY06_9CAUD DNA primase OS=Caulobacter phage Jess A OX=2584753 GN=JessAGP_004c PE=4 SV=1
MM1 pKa = 7.75PFITLPTASGNVLINTVHH19 pKa = 5.96IVSIRR24 pKa = 11.84VAPDD28 pKa = 2.67NGAYY32 pKa = 10.36VDD34 pKa = 3.79VTGGNVYY41 pKa = 8.59TATLPPTAQGVLEE54 pKa = 5.03LIEE57 pKa = 4.21AAEE60 pKa = 4.38DD61 pKa = 3.65EE62 pKa = 4.59EE63 pKa = 5.22AAEE66 pKa = 4.1QQ67 pKa = 3.76

Molecular weight: 6.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5NY25|A0A4Y5NY25_9CAUD Terminase large subunit OS=Caulobacter phage Jess A OX=2584753 GN=JessAGP_001c PE=4 SV=1
MM1 pKa = 6.73STSKK5 pKa = 10.43IFLTCPEE12 pKa = 4.65CGQKK16 pKa = 10.33FEE18 pKa = 4.96AKK20 pKa = 8.89HH21 pKa = 5.71HH22 pKa = 6.02RR23 pKa = 11.84ASFCSPAHH31 pKa = 5.74RR32 pKa = 11.84NAYY35 pKa = 10.06NNRR38 pKa = 11.84QLAEE42 pKa = 4.02GQRR45 pKa = 11.84IVAIAKK51 pKa = 9.1AWRR54 pKa = 11.84QSRR57 pKa = 11.84STSDD61 pKa = 3.46PALKK65 pKa = 10.48AAGRR69 pKa = 11.84EE70 pKa = 4.23AFSQLCRR77 pKa = 11.84EE78 pKa = 4.12LDD80 pKa = 3.51MLNAADD86 pKa = 3.97SAHH89 pKa = 5.71GRR91 pKa = 11.84MSALKK96 pKa = 9.2VYY98 pKa = 10.32RR99 pKa = 11.84RR100 pKa = 11.84RR101 pKa = 11.84SANGRR106 pKa = 11.84LDD108 pKa = 3.39HH109 pKa = 6.41QGPLAAAAKK118 pKa = 10.25SRR120 pKa = 3.58

Molecular weight: 13.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

13663

62

2039

253.0

27.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.545 ± 0.463

0.695 ± 0.131

6.477 ± 0.259

5.914 ± 0.349

3.169 ± 0.182

8.153 ± 0.28

1.566 ± 0.151

4.077 ± 0.194

4.728 ± 0.257

8.805 ± 0.271

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.276 ± 0.137

3.762 ± 0.316

4.911 ± 0.186

4.457 ± 0.274

6.287 ± 0.319

5.204 ± 0.292

6.031 ± 0.415

6.47 ± 0.25

1.837 ± 0.165

2.635 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski