Amino acid dipepetide frequency for Caulobacter phage Jess A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.03AlaAla: 16.03 ± 1.294
0.805AlaCys: 0.805 ± 0.293
7.612AlaAsp: 7.612 ± 0.657
7.539AlaGlu: 7.539 ± 0.807
3.733AlaPhe: 3.733 ± 0.501
9.003AlaGly: 9.003 ± 0.934
1.464AlaHis: 1.464 ± 0.335
5.124AlaIle: 5.124 ± 0.579
6.514AlaLys: 6.514 ± 0.796
11.272AlaLeu: 11.272 ± 1.295
2.781AlaMet: 2.781 ± 0.384
4.831AlaAsn: 4.831 ± 1.651
5.416AlaPro: 5.416 ± 0.954
7.32AlaGln: 7.32 ± 1.044
7.246AlaArg: 7.246 ± 0.722
7.466AlaSer: 7.466 ± 0.953
7.686AlaThr: 7.686 ± 1.081
7.246AlaVal: 7.246 ± 0.979
3.147AlaTrp: 3.147 ± 0.544
3.221AlaTyr: 3.221 ± 0.552
0.0AlaXaa: 0.0 ± 0.0
Cys
0.586CysAla: 0.586 ± 0.244
0.073CysCys: 0.073 ± 0.078
0.366CysAsp: 0.366 ± 0.168
0.512CysGlu: 0.512 ± 0.204
0.22CysPhe: 0.22 ± 0.115
0.659CysGly: 0.659 ± 0.225
0.073CysHis: 0.073 ± 0.078
0.439CysIle: 0.439 ± 0.174
0.22CysLys: 0.22 ± 0.138
0.439CysLeu: 0.439 ± 0.187
0.293CysMet: 0.293 ± 0.152
0.22CysAsn: 0.22 ± 0.122
0.586CysPro: 0.586 ± 0.222
0.22CysGln: 0.22 ± 0.131
0.512CysArg: 0.512 ± 0.161
0.366CysSer: 0.366 ± 0.187
0.22CysThr: 0.22 ± 0.125
0.512CysVal: 0.512 ± 0.198
0.073CysTrp: 0.073 ± 0.059
0.366CysTyr: 0.366 ± 0.155
0.0CysXaa: 0.0 ± 0.0
Asp
7.978AspAla: 7.978 ± 0.862
0.22AspCys: 0.22 ± 0.129
4.538AspAsp: 4.538 ± 0.725
4.831AspGlu: 4.831 ± 0.62
2.342AspPhe: 2.342 ± 0.302
6.148AspGly: 6.148 ± 1.064
1.025AspHis: 1.025 ± 0.272
2.562AspIle: 2.562 ± 0.452
2.928AspLys: 2.928 ± 0.513
5.416AspLeu: 5.416 ± 0.529
2.049AspMet: 2.049 ± 0.37
2.415AspAsn: 2.415 ± 0.396
3.66AspPro: 3.66 ± 0.631
2.708AspGln: 2.708 ± 0.36
3.806AspArg: 3.806 ± 0.475
2.342AspSer: 2.342 ± 0.443
3.44AspThr: 3.44 ± 0.51
3.879AspVal: 3.879 ± 0.529
1.098AspTrp: 1.098 ± 0.268
1.391AspTyr: 1.391 ± 0.315
0.0AspXaa: 0.0 ± 0.0
Glu
9.003GluAla: 9.003 ± 0.877
0.512GluCys: 0.512 ± 0.237
4.026GluAsp: 4.026 ± 0.738
2.635GluGlu: 2.635 ± 0.566
1.903GluPhe: 1.903 ± 0.364
4.026GluGly: 4.026 ± 0.433
1.025GluHis: 1.025 ± 0.435
3.001GluIle: 3.001 ± 0.453
2.342GluLys: 2.342 ± 0.534
6.807GluLeu: 6.807 ± 0.856
1.025GluMet: 1.025 ± 0.37
1.903GluAsn: 1.903 ± 0.448
2.635GluPro: 2.635 ± 0.432
2.855GluGln: 2.855 ± 0.356
4.611GluArg: 4.611 ± 0.689
2.269GluSer: 2.269 ± 0.422
3.733GluThr: 3.733 ± 0.48
2.855GluVal: 2.855 ± 0.505
0.878GluTrp: 0.878 ± 0.24
1.098GluTyr: 1.098 ± 0.283
0.0GluXaa: 0.0 ± 0.0
Phe
3.001PheAla: 3.001 ± 0.58
0.586PheCys: 0.586 ± 0.247
3.147PheAsp: 3.147 ± 0.419
2.269PheGlu: 2.269 ± 0.464
1.025PhePhe: 1.025 ± 0.211
2.635PheGly: 2.635 ± 0.411
0.293PheHis: 0.293 ± 0.14
1.171PheIle: 1.171 ± 0.292
2.415PheLys: 2.415 ± 0.387
1.537PheLeu: 1.537 ± 0.398
0.732PheMet: 0.732 ± 0.205
1.244PheAsn: 1.244 ± 0.347
1.025PhePro: 1.025 ± 0.271
1.318PheGln: 1.318 ± 0.233
1.391PheArg: 1.391 ± 0.318
2.342PheSer: 2.342 ± 0.438
2.196PheThr: 2.196 ± 0.381
1.684PheVal: 1.684 ± 0.314
0.805PheTrp: 0.805 ± 0.266
0.878PheTyr: 0.878 ± 0.243
0.0PheXaa: 0.0 ± 0.0
Gly
9.369GlyAla: 9.369 ± 1.591
1.025GlyCys: 1.025 ± 0.376
4.758GlyAsp: 4.758 ± 0.655
4.904GlyGlu: 4.904 ± 0.625
3.147GlyPhe: 3.147 ± 0.454
6.588GlyGly: 6.588 ± 0.676
1.684GlyHis: 1.684 ± 0.36
2.269GlyIle: 2.269 ± 0.363
4.245GlyLys: 4.245 ± 0.482
7.32GlyLeu: 7.32 ± 0.757
1.83GlyMet: 1.83 ± 0.36
2.928GlyAsn: 2.928 ± 0.515
2.781GlyPro: 2.781 ± 0.395
4.319GlyGln: 4.319 ± 0.587
4.831GlyArg: 4.831 ± 0.72
3.44GlySer: 3.44 ± 0.502
4.611GlyThr: 4.611 ± 0.842
7.393GlyVal: 7.393 ± 0.696
1.025GlyTrp: 1.025 ± 0.304
3.074GlyTyr: 3.074 ± 0.497
0.0GlyXaa: 0.0 ± 0.0
His
1.61HisAla: 1.61 ± 0.453
0.293HisCys: 0.293 ± 0.153
1.244HisAsp: 1.244 ± 0.284
0.952HisGlu: 0.952 ± 0.229
0.659HisPhe: 0.659 ± 0.186
0.952HisGly: 0.952 ± 0.243
0.439HisHis: 0.439 ± 0.159
0.293HisIle: 0.293 ± 0.151
0.805HisLys: 0.805 ± 0.303
1.244HisLeu: 1.244 ± 0.341
0.22HisMet: 0.22 ± 0.14
0.732HisAsn: 0.732 ± 0.242
1.464HisPro: 1.464 ± 0.306
0.732HisGln: 0.732 ± 0.212
0.805HisArg: 0.805 ± 0.274
0.952HisSer: 0.952 ± 0.243
0.512HisThr: 0.512 ± 0.177
0.878HisVal: 0.878 ± 0.241
0.366HisTrp: 0.366 ± 0.191
0.512HisTyr: 0.512 ± 0.197
0.0HisXaa: 0.0 ± 0.0
Ile
4.611IleAla: 4.611 ± 0.595
0.439IleCys: 0.439 ± 0.2
2.415IleAsp: 2.415 ± 0.45
2.855IleGlu: 2.855 ± 0.543
0.512IlePhe: 0.512 ± 0.189
3.147IleGly: 3.147 ± 0.499
0.952IleHis: 0.952 ± 0.284
1.757IleIle: 1.757 ± 0.416
1.537IleLys: 1.537 ± 0.325
3.587IleLeu: 3.587 ± 0.504
0.732IleMet: 0.732 ± 0.209
2.049IleAsn: 2.049 ± 0.332
2.049IlePro: 2.049 ± 0.418
2.049IleGln: 2.049 ± 0.349
2.928IleArg: 2.928 ± 0.446
2.415IleSer: 2.415 ± 0.497
2.781IleThr: 2.781 ± 0.425
2.342IleVal: 2.342 ± 0.354
0.805IleTrp: 0.805 ± 0.217
0.805IleTyr: 0.805 ± 0.38
0.0IleXaa: 0.0 ± 0.0
Lys
8.71LysAla: 8.71 ± 1.03
0.22LysCys: 0.22 ± 0.144
3.66LysAsp: 3.66 ± 0.565
1.976LysGlu: 1.976 ± 0.434
0.952LysPhe: 0.952 ± 0.289
3.953LysGly: 3.953 ± 0.685
0.586LysHis: 0.586 ± 0.216
1.903LysIle: 1.903 ± 0.453
1.757LysLys: 1.757 ± 0.444
4.538LysLeu: 4.538 ± 0.584
0.512LysMet: 0.512 ± 0.185
1.684LysAsn: 1.684 ± 0.355
2.489LysPro: 2.489 ± 0.455
0.878LysGln: 0.878 ± 0.226
2.855LysArg: 2.855 ± 0.414
2.269LysSer: 2.269 ± 0.374
2.635LysThr: 2.635 ± 0.418
3.66LysVal: 3.66 ± 0.64
0.805LysTrp: 0.805 ± 0.241
1.244LysTyr: 1.244 ± 0.273
0.0LysXaa: 0.0 ± 0.0
Leu
11.638LeuAla: 11.638 ± 0.961
0.439LeuCys: 0.439 ± 0.173
6.441LeuAsp: 6.441 ± 0.967
5.343LeuGlu: 5.343 ± 0.407
1.83LeuPhe: 1.83 ± 0.292
7.466LeuGly: 7.466 ± 0.74
1.098LeuHis: 1.098 ± 0.251
2.781LeuIle: 2.781 ± 0.435
4.538LeuLys: 4.538 ± 0.579
6.954LeuLeu: 6.954 ± 0.628
2.123LeuMet: 2.123 ± 0.514
3.221LeuAsn: 3.221 ± 0.566
5.27LeuPro: 5.27 ± 0.674
3.44LeuGln: 3.44 ± 0.382
5.343LeuArg: 5.343 ± 0.535
4.831LeuSer: 4.831 ± 0.62
6.002LeuThr: 6.002 ± 0.822
5.856LeuVal: 5.856 ± 0.6
1.171LeuTrp: 1.171 ± 0.252
2.269LeuTyr: 2.269 ± 0.643
0.0LeuXaa: 0.0 ± 0.0
Met
3.221MetAla: 3.221 ± 0.493
0.366MetCys: 0.366 ± 0.173
1.391MetAsp: 1.391 ± 0.257
1.025MetGlu: 1.025 ± 0.305
0.366MetPhe: 0.366 ± 0.144
1.318MetGly: 1.318 ± 0.236
0.366MetHis: 0.366 ± 0.143
0.952MetIle: 0.952 ± 0.239
0.586MetLys: 0.586 ± 0.184
2.049MetLeu: 2.049 ± 0.432
0.586MetMet: 0.586 ± 0.241
1.098MetAsn: 1.098 ± 0.306
1.171MetPro: 1.171 ± 0.249
1.098MetGln: 1.098 ± 0.28
2.196MetArg: 2.196 ± 0.418
1.318MetSer: 1.318 ± 0.311
1.903MetThr: 1.903 ± 0.38
1.171MetVal: 1.171 ± 0.234
0.22MetTrp: 0.22 ± 0.147
0.366MetTyr: 0.366 ± 0.141
0.0MetXaa: 0.0 ± 0.0
Asn
4.831AsnAla: 4.831 ± 0.788
0.073AsnCys: 0.073 ± 0.078
1.757AsnAsp: 1.757 ± 0.304
1.903AsnGlu: 1.903 ± 0.347
0.878AsnPhe: 0.878 ± 0.233
3.66AsnGly: 3.66 ± 0.57
0.293AsnHis: 0.293 ± 0.124
2.196AsnIle: 2.196 ± 0.361
0.878AsnLys: 0.878 ± 0.271
4.611AsnLeu: 4.611 ± 1.748
1.464AsnMet: 1.464 ± 0.265
1.244AsnAsn: 1.244 ± 0.284
1.757AsnPro: 1.757 ± 0.327
2.123AsnGln: 2.123 ± 0.417
1.976AsnArg: 1.976 ± 0.517
1.83AsnSer: 1.83 ± 0.343
1.976AsnThr: 1.976 ± 0.389
2.708AsnVal: 2.708 ± 0.492
0.293AsnTrp: 0.293 ± 0.115
1.171AsnTyr: 1.171 ± 0.363
0.0AsnXaa: 0.0 ± 0.0
Pro
5.343ProAla: 5.343 ± 0.615
0.22ProCys: 0.22 ± 0.129
4.026ProAsp: 4.026 ± 0.52
3.513ProGlu: 3.513 ± 0.514
1.537ProPhe: 1.537 ± 0.357
3.953ProGly: 3.953 ± 0.561
0.878ProHis: 0.878 ± 0.236
1.244ProIle: 1.244 ± 0.253
3.513ProLys: 3.513 ± 0.486
3.221ProLeu: 3.221 ± 0.415
0.878ProMet: 0.878 ± 0.19
2.049ProAsn: 2.049 ± 0.328
2.196ProPro: 2.196 ± 0.508
2.049ProGln: 2.049 ± 0.403
2.635ProArg: 2.635 ± 0.532
2.781ProSer: 2.781 ± 0.406
3.513ProThr: 3.513 ± 0.562
3.44ProVal: 3.44 ± 0.496
1.025ProTrp: 1.025 ± 0.261
1.098ProTyr: 1.098 ± 0.336
0.0ProXaa: 0.0 ± 0.0
Gln
6.661GlnAla: 6.661 ± 1.162
0.073GlnCys: 0.073 ± 0.078
1.757GlnAsp: 1.757 ± 0.365
2.123GlnGlu: 2.123 ± 0.422
1.244GlnPhe: 1.244 ± 0.271
4.319GlnGly: 4.319 ± 0.516
0.805GlnHis: 0.805 ± 0.2
2.123GlnIle: 2.123 ± 0.382
1.318GlnLys: 1.318 ± 0.285
4.172GlnLeu: 4.172 ± 0.573
1.025GlnMet: 1.025 ± 0.239
1.171GlnAsn: 1.171 ± 0.283
2.049GlnPro: 2.049 ± 0.385
2.635GlnGln: 2.635 ± 0.54
3.66GlnArg: 3.66 ± 0.504
1.244GlnSer: 1.244 ± 0.334
3.074GlnThr: 3.074 ± 0.475
3.074GlnVal: 3.074 ± 0.542
0.732GlnTrp: 0.732 ± 0.198
1.318GlnTyr: 1.318 ± 0.347
0.0GlnXaa: 0.0 ± 0.0
Arg
7.32ArgAla: 7.32 ± 0.802
0.293ArgCys: 0.293 ± 0.157
3.953ArgAsp: 3.953 ± 0.638
4.538ArgGlu: 4.538 ± 0.457
2.708ArgPhe: 2.708 ± 0.525
3.074ArgGly: 3.074 ± 0.416
1.098ArgHis: 1.098 ± 0.285
3.806ArgIle: 3.806 ± 0.476
3.953ArgLys: 3.953 ± 0.549
5.636ArgLeu: 5.636 ± 0.678
1.244ArgMet: 1.244 ± 0.292
1.976ArgAsn: 1.976 ± 0.33
2.415ArgPro: 2.415 ± 0.398
2.855ArgGln: 2.855 ± 0.425
3.879ArgArg: 3.879 ± 0.63
3.879ArgSer: 3.879 ± 0.483
2.708ArgThr: 2.708 ± 0.36
3.806ArgVal: 3.806 ± 0.414
1.903ArgTrp: 1.903 ± 0.56
1.83ArgTyr: 1.83 ± 0.316
0.0ArgXaa: 0.0 ± 0.0
Ser
5.636SerAla: 5.636 ± 0.66
0.366SerCys: 0.366 ± 0.157
3.733SerAsp: 3.733 ± 0.606
2.415SerGlu: 2.415 ± 0.426
2.196SerPhe: 2.196 ± 0.505
5.636SerGly: 5.636 ± 0.643
0.732SerHis: 0.732 ± 0.294
2.928SerIle: 2.928 ± 0.45
2.123SerLys: 2.123 ± 0.329
4.026SerLeu: 4.026 ± 0.511
0.805SerMet: 0.805 ± 0.239
2.415SerAsn: 2.415 ± 0.382
3.294SerPro: 3.294 ± 0.4
1.391SerGln: 1.391 ± 0.34
3.001SerArg: 3.001 ± 0.462
3.221SerSer: 3.221 ± 0.549
3.513SerThr: 3.513 ± 0.484
2.781SerVal: 2.781 ± 0.369
0.952SerTrp: 0.952 ± 0.224
0.878SerTyr: 0.878 ± 0.187
0.0SerXaa: 0.0 ± 0.0
Thr
6.954ThrAla: 6.954 ± 0.761
0.439ThrCys: 0.439 ± 0.204
3.367ThrAsp: 3.367 ± 0.546
2.781ThrGlu: 2.781 ± 0.531
1.976ThrPhe: 1.976 ± 0.347
5.929ThrGly: 5.929 ± 0.7
1.098ThrHis: 1.098 ± 0.305
2.562ThrIle: 2.562 ± 0.41
2.489ThrLys: 2.489 ± 0.374
5.343ThrLeu: 5.343 ± 0.56
1.318ThrMet: 1.318 ± 0.299
2.049ThrAsn: 2.049 ± 0.634
3.294ThrPro: 3.294 ± 0.394
2.855ThrGln: 2.855 ± 0.483
3.294ThrArg: 3.294 ± 0.454
3.66ThrSer: 3.66 ± 0.465
3.221ThrThr: 3.221 ± 0.65
4.611ThrVal: 4.611 ± 0.959
1.318ThrTrp: 1.318 ± 0.366
1.757ThrTyr: 1.757 ± 0.417
0.0ThrXaa: 0.0 ± 0.0
Val
7.027ValAla: 7.027 ± 0.89
0.146ValCys: 0.146 ± 0.151
4.685ValAsp: 4.685 ± 0.492
4.831ValGlu: 4.831 ± 0.579
2.562ValPhe: 2.562 ± 0.453
3.953ValGly: 3.953 ± 0.496
1.171ValHis: 1.171 ± 0.287
1.903ValIle: 1.903 ± 0.497
2.708ValLys: 2.708 ± 0.534
5.197ValLeu: 5.197 ± 0.772
2.196ValMet: 2.196 ± 0.39
2.928ValAsn: 2.928 ± 0.586
3.66ValPro: 3.66 ± 0.449
1.976ValGln: 1.976 ± 0.516
5.051ValArg: 5.051 ± 0.543
3.367ValSer: 3.367 ± 0.553
4.465ValThr: 4.465 ± 0.598
4.026ValVal: 4.026 ± 0.641
0.732ValTrp: 0.732 ± 0.232
2.049ValTyr: 2.049 ± 0.317
0.0ValXaa: 0.0 ± 0.0
Trp
2.269TrpAla: 2.269 ± 0.339
0.146TrpCys: 0.146 ± 0.096
0.732TrpAsp: 0.732 ± 0.244
0.878TrpGlu: 0.878 ± 0.247
1.318TrpPhe: 1.318 ± 0.32
1.83TrpGly: 1.83 ± 0.343
0.073TrpHis: 0.073 ± 0.078
0.586TrpIle: 0.586 ± 0.183
1.391TrpLys: 1.391 ± 0.389
2.562TrpLeu: 2.562 ± 0.457
0.293TrpMet: 0.293 ± 0.107
0.439TrpAsn: 0.439 ± 0.173
0.878TrpPro: 0.878 ± 0.254
0.732TrpGln: 0.732 ± 0.194
1.318TrpArg: 1.318 ± 0.378
0.732TrpSer: 0.732 ± 0.292
0.586TrpThr: 0.586 ± 0.232
0.659TrpVal: 0.659 ± 0.198
0.439TrpTrp: 0.439 ± 0.17
0.512TrpTyr: 0.512 ± 0.214
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.66TyrAla: 3.66 ± 0.39
0.22TyrCys: 0.22 ± 0.13
1.171TyrAsp: 1.171 ± 0.37
1.318TyrGlu: 1.318 ± 0.33
0.586TyrPhe: 0.586 ± 0.177
3.294TyrGly: 3.294 ± 0.62
0.512TyrHis: 0.512 ± 0.192
1.171TyrIle: 1.171 ± 0.272
1.025TyrLys: 1.025 ± 0.242
2.123TyrLeu: 2.123 ± 0.493
0.586TyrMet: 0.586 ± 0.183
1.025TyrAsn: 1.025 ± 0.216
1.025TyrPro: 1.025 ± 0.3
1.025TyrGln: 1.025 ± 0.228
1.537TyrArg: 1.537 ± 0.409
1.318TyrSer: 1.318 ± 0.415
1.537TyrThr: 1.537 ± 0.291
2.123TyrVal: 2.123 ± 0.561
0.586TyrTrp: 0.586 ± 0.154
0.512TyrTyr: 0.512 ± 0.179
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 54 proteins (13663 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski