Amino acid dipepetide frequency for Ralstonia phage RsoM1USA

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.404AlaAla: 21.404 ± 2.342
0.666AlaCys: 0.666 ± 0.233
8.412AlaAsp: 8.412 ± 1.008
7.079AlaGlu: 7.079 ± 0.642
3.498AlaPhe: 3.498 ± 0.647
11.66AlaGly: 11.66 ± 0.736
2.998AlaHis: 2.998 ± 0.538
5.164AlaIle: 5.164 ± 0.496
4.581AlaLys: 4.581 ± 0.871
12.493AlaLeu: 12.493 ± 1.269
3.498AlaMet: 3.498 ± 0.485
3.581AlaAsn: 3.581 ± 0.49
5.247AlaPro: 5.247 ± 0.657
4.664AlaGln: 4.664 ± 0.578
9.078AlaArg: 9.078 ± 0.925
6.08AlaSer: 6.08 ± 0.792
6.496AlaThr: 6.496 ± 0.66
9.744AlaVal: 9.744 ± 0.945
2.249AlaTrp: 2.249 ± 0.392
3.581AlaTyr: 3.581 ± 0.485
0.0AlaXaa: 0.0 ± 0.0
Cys
1.083CysAla: 1.083 ± 0.393
0.083CysCys: 0.083 ± 0.093
0.416CysAsp: 0.416 ± 0.237
0.833CysGlu: 0.833 ± 0.303
0.083CysPhe: 0.083 ± 0.09
0.666CysGly: 0.666 ± 0.24
0.167CysHis: 0.167 ± 0.113
0.583CysIle: 0.583 ± 0.221
0.167CysLys: 0.167 ± 0.115
0.5CysLeu: 0.5 ± 0.222
0.666CysMet: 0.666 ± 0.231
0.25CysAsn: 0.25 ± 0.125
0.833CysPro: 0.833 ± 0.287
0.25CysGln: 0.25 ± 0.138
0.416CysArg: 0.416 ± 0.224
0.583CysSer: 0.583 ± 0.23
0.75CysThr: 0.75 ± 0.264
0.5CysVal: 0.5 ± 0.197
0.0CysTrp: 0.0 ± 0.0
0.25CysTyr: 0.25 ± 0.122
0.0CysXaa: 0.0 ± 0.0
Asp
8.995AspAla: 8.995 ± 0.849
0.333AspCys: 0.333 ± 0.152
2.832AspAsp: 2.832 ± 0.712
2.832AspGlu: 2.832 ± 0.471
1.499AspPhe: 1.499 ± 0.362
5.58AspGly: 5.58 ± 0.74
1.083AspHis: 1.083 ± 0.361
2.165AspIle: 2.165 ± 0.629
2.665AspLys: 2.665 ± 0.609
5.33AspLeu: 5.33 ± 0.705
0.833AspMet: 0.833 ± 0.262
1.749AspAsn: 1.749 ± 0.413
3.665AspPro: 3.665 ± 0.545
2.665AspGln: 2.665 ± 0.344
4.581AspArg: 4.581 ± 0.527
1.999AspSer: 1.999 ± 0.406
3.248AspThr: 3.248 ± 0.495
4.164AspVal: 4.164 ± 0.554
0.75AspTrp: 0.75 ± 0.269
1.333AspTyr: 1.333 ± 0.343
0.0AspXaa: 0.0 ± 0.0
Glu
6.163GluAla: 6.163 ± 0.779
0.666GluCys: 0.666 ± 0.231
1.749GluAsp: 1.749 ± 0.342
2.748GluGlu: 2.748 ± 0.52
1.416GluPhe: 1.416 ± 0.278
3.165GluGly: 3.165 ± 0.522
1.249GluHis: 1.249 ± 0.288
2.332GluIle: 2.332 ± 0.636
2.665GluLys: 2.665 ± 0.438
6.579GluLeu: 6.579 ± 0.666
1.166GluMet: 1.166 ± 0.287
1.582GluAsn: 1.582 ± 0.341
2.915GluPro: 2.915 ± 0.468
2.415GluGln: 2.415 ± 0.524
5.414GluArg: 5.414 ± 0.695
2.082GluSer: 2.082 ± 0.378
2.332GluThr: 2.332 ± 0.418
3.831GluVal: 3.831 ± 0.637
1.083GluTrp: 1.083 ± 0.359
1.582GluTyr: 1.582 ± 0.412
0.0GluXaa: 0.0 ± 0.0
Phe
4.414PheAla: 4.414 ± 0.612
0.25PheCys: 0.25 ± 0.134
1.999PheAsp: 1.999 ± 0.407
1.666PheGlu: 1.666 ± 0.296
0.999PhePhe: 0.999 ± 0.289
2.165PheGly: 2.165 ± 0.38
0.416PheHis: 0.416 ± 0.157
1.249PheIle: 1.249 ± 0.303
1.166PheLys: 1.166 ± 0.239
1.916PheLeu: 1.916 ± 0.366
0.5PheMet: 0.5 ± 0.204
0.75PheAsn: 0.75 ± 0.207
1.333PhePro: 1.333 ± 0.341
1.166PheGln: 1.166 ± 0.284
2.832PheArg: 2.832 ± 0.541
2.748PheSer: 2.748 ± 0.761
2.165PheThr: 2.165 ± 0.381
2.415PheVal: 2.415 ± 0.464
0.916PheTrp: 0.916 ± 0.269
0.416PheTyr: 0.416 ± 0.201
0.0PheXaa: 0.0 ± 0.0
Gly
8.828GlyAla: 8.828 ± 1.149
0.583GlyCys: 0.583 ± 0.218
5.663GlyAsp: 5.663 ± 0.721
3.581GlyGlu: 3.581 ± 0.551
2.665GlyPhe: 2.665 ± 0.361
5.747GlyGly: 5.747 ± 0.643
1.166GlyHis: 1.166 ± 0.376
3.082GlyIle: 3.082 ± 0.509
3.748GlyLys: 3.748 ± 0.617
5.414GlyLeu: 5.414 ± 0.863
2.082GlyMet: 2.082 ± 0.415
2.082GlyAsn: 2.082 ± 0.387
3.165GlyPro: 3.165 ± 0.546
2.832GlyGln: 2.832 ± 0.449
5.164GlyArg: 5.164 ± 0.607
3.082GlySer: 3.082 ± 0.533
5.08GlyThr: 5.08 ± 0.74
6.163GlyVal: 6.163 ± 0.813
1.416GlyTrp: 1.416 ± 0.282
2.665GlyTyr: 2.665 ± 0.459
0.0GlyXaa: 0.0 ± 0.0
His
2.165HisAla: 2.165 ± 0.583
0.416HisCys: 0.416 ± 0.176
1.416HisAsp: 1.416 ± 0.312
1.083HisGlu: 1.083 ± 0.298
0.5HisPhe: 0.5 ± 0.21
2.082HisGly: 2.082 ± 0.439
0.75HisHis: 0.75 ± 0.216
0.999HisIle: 0.999 ± 0.284
0.75HisLys: 0.75 ± 0.29
1.416HisLeu: 1.416 ± 0.28
0.833HisMet: 0.833 ± 0.25
0.75HisAsn: 0.75 ± 0.21
1.333HisPro: 1.333 ± 0.376
0.999HisGln: 0.999 ± 0.284
1.333HisArg: 1.333 ± 0.309
1.166HisSer: 1.166 ± 0.285
1.249HisThr: 1.249 ± 0.407
1.416HisVal: 1.416 ± 0.256
0.167HisTrp: 0.167 ± 0.108
0.833HisTyr: 0.833 ± 0.279
0.0HisXaa: 0.0 ± 0.0
Ile
5.58IleAla: 5.58 ± 0.617
0.25IleCys: 0.25 ± 0.15
3.748IleAsp: 3.748 ± 0.565
3.331IleGlu: 3.331 ± 0.499
0.583IlePhe: 0.583 ± 0.212
3.581IleGly: 3.581 ± 0.529
0.833IleHis: 0.833 ± 0.221
1.166IleIle: 1.166 ± 0.382
1.499IleLys: 1.499 ± 0.364
3.082IleLeu: 3.082 ± 0.449
0.583IleMet: 0.583 ± 0.181
1.582IleAsn: 1.582 ± 0.313
1.416IlePro: 1.416 ± 0.384
0.75IleGln: 0.75 ± 0.302
2.748IleArg: 2.748 ± 0.486
2.165IleSer: 2.165 ± 0.462
3.248IleThr: 3.248 ± 0.558
3.415IleVal: 3.415 ± 0.465
0.75IleTrp: 0.75 ± 0.261
0.5IleTyr: 0.5 ± 0.229
0.0IleXaa: 0.0 ± 0.0
Lys
6.246LysAla: 6.246 ± 0.907
0.25LysCys: 0.25 ± 0.158
2.082LysAsp: 2.082 ± 0.348
1.916LysGlu: 1.916 ± 0.502
1.499LysPhe: 1.499 ± 0.388
2.915LysGly: 2.915 ± 0.403
0.833LysHis: 0.833 ± 0.262
1.083LysIle: 1.083 ± 0.265
2.165LysLys: 2.165 ± 0.537
3.665LysLeu: 3.665 ± 0.648
0.333LysMet: 0.333 ± 0.189
0.999LysAsn: 0.999 ± 0.217
2.415LysPro: 2.415 ± 0.386
1.916LysGln: 1.916 ± 0.379
3.165LysArg: 3.165 ± 0.495
1.999LysSer: 1.999 ± 0.561
2.582LysThr: 2.582 ± 0.393
2.748LysVal: 2.748 ± 0.654
0.416LysTrp: 0.416 ± 0.197
0.666LysTyr: 0.666 ± 0.231
0.0LysXaa: 0.0 ± 0.0
Leu
14.408LeuAla: 14.408 ± 1.003
1.083LeuCys: 1.083 ± 0.324
4.497LeuAsp: 4.497 ± 0.701
5.663LeuGlu: 5.663 ± 0.656
2.832LeuPhe: 2.832 ± 0.621
4.914LeuGly: 4.914 ± 0.736
2.415LeuHis: 2.415 ± 0.476
3.498LeuIle: 3.498 ± 0.516
3.498LeuLys: 3.498 ± 0.562
7.912LeuLeu: 7.912 ± 0.815
1.832LeuMet: 1.832 ± 0.445
2.082LeuAsn: 2.082 ± 0.455
5.247LeuPro: 5.247 ± 0.635
3.165LeuGln: 3.165 ± 0.514
7.329LeuArg: 7.329 ± 0.819
6.496LeuSer: 6.496 ± 0.721
5.58LeuThr: 5.58 ± 0.811
6.163LeuVal: 6.163 ± 0.698
0.999LeuTrp: 0.999 ± 0.247
2.082LeuTyr: 2.082 ± 0.382
0.0LeuXaa: 0.0 ± 0.0
Met
3.415MetAla: 3.415 ± 0.456
0.167MetCys: 0.167 ± 0.104
1.499MetAsp: 1.499 ± 0.428
0.833MetGlu: 0.833 ± 0.287
0.583MetPhe: 0.583 ± 0.186
0.916MetGly: 0.916 ± 0.26
0.5MetHis: 0.5 ± 0.231
0.333MetIle: 0.333 ± 0.173
1.333MetLys: 1.333 ± 0.365
2.582MetLeu: 2.582 ± 0.418
0.5MetMet: 0.5 ± 0.224
0.666MetAsn: 0.666 ± 0.231
1.083MetPro: 1.083 ± 0.251
0.5MetGln: 0.5 ± 0.197
1.582MetArg: 1.582 ± 0.305
1.416MetSer: 1.416 ± 0.302
1.999MetThr: 1.999 ± 0.381
1.749MetVal: 1.749 ± 0.311
0.083MetTrp: 0.083 ± 0.087
0.5MetTyr: 0.5 ± 0.177
0.0MetXaa: 0.0 ± 0.0
Asn
3.831AsnAla: 3.831 ± 0.418
0.167AsnCys: 0.167 ± 0.101
2.249AsnAsp: 2.249 ± 0.442
1.083AsnGlu: 1.083 ± 0.242
0.833AsnPhe: 0.833 ± 0.225
3.165AsnGly: 3.165 ± 0.566
0.666AsnHis: 0.666 ± 0.222
1.083AsnIle: 1.083 ± 0.251
1.416AsnLys: 1.416 ± 0.372
3.082AsnLeu: 3.082 ± 0.547
0.416AsnMet: 0.416 ± 0.176
0.583AsnAsn: 0.583 ± 0.198
2.832AsnPro: 2.832 ± 0.407
1.249AsnGln: 1.249 ± 0.314
1.333AsnArg: 1.333 ± 0.247
1.166AsnSer: 1.166 ± 0.265
1.499AsnThr: 1.499 ± 0.303
1.166AsnVal: 1.166 ± 0.245
0.167AsnTrp: 0.167 ± 0.113
0.999AsnTyr: 0.999 ± 0.277
0.0AsnXaa: 0.0 ± 0.0
Pro
7.162ProAla: 7.162 ± 0.886
0.5ProCys: 0.5 ± 0.208
3.165ProAsp: 3.165 ± 0.512
3.082ProGlu: 3.082 ± 0.479
1.416ProPhe: 1.416 ± 0.338
3.331ProGly: 3.331 ± 0.425
1.333ProHis: 1.333 ± 0.291
2.499ProIle: 2.499 ± 0.415
1.499ProLys: 1.499 ± 0.341
4.997ProLeu: 4.997 ± 0.789
1.249ProMet: 1.249 ± 0.253
1.416ProAsn: 1.416 ± 0.285
2.249ProPro: 2.249 ± 0.438
1.416ProGln: 1.416 ± 0.346
2.998ProArg: 2.998 ± 0.483
3.165ProSer: 3.165 ± 0.505
2.665ProThr: 2.665 ± 0.531
3.748ProVal: 3.748 ± 0.486
1.166ProTrp: 1.166 ± 0.29
1.166ProTyr: 1.166 ± 0.281
0.0ProXaa: 0.0 ± 0.0
Gln
4.831GlnAla: 4.831 ± 0.66
0.416GlnCys: 0.416 ± 0.215
2.082GlnAsp: 2.082 ± 0.415
2.082GlnGlu: 2.082 ± 0.54
1.666GlnPhe: 1.666 ± 0.383
1.416GlnGly: 1.416 ± 0.356
0.416GlnHis: 0.416 ± 0.139
1.333GlnIle: 1.333 ± 0.332
1.249GlnLys: 1.249 ± 0.422
4.997GlnLeu: 4.997 ± 0.557
0.666GlnMet: 0.666 ± 0.195
0.833GlnAsn: 0.833 ± 0.255
1.832GlnPro: 1.832 ± 0.349
2.332GlnGln: 2.332 ± 0.454
4.248GlnArg: 4.248 ± 0.481
1.749GlnSer: 1.749 ± 0.341
2.499GlnThr: 2.499 ± 0.461
2.665GlnVal: 2.665 ± 0.547
0.833GlnTrp: 0.833 ± 0.23
1.416GlnTyr: 1.416 ± 0.312
0.0GlnXaa: 0.0 ± 0.0
Arg
8.745ArgAla: 8.745 ± 1.051
0.416ArgCys: 0.416 ± 0.169
3.914ArgAsp: 3.914 ± 0.423
4.497ArgGlu: 4.497 ± 0.634
2.332ArgPhe: 2.332 ± 0.374
5.247ArgGly: 5.247 ± 0.672
2.082ArgHis: 2.082 ± 0.447
3.748ArgIle: 3.748 ± 0.588
2.832ArgLys: 2.832 ± 0.462
7.246ArgLeu: 7.246 ± 0.765
1.582ArgMet: 1.582 ± 0.307
2.499ArgAsn: 2.499 ± 0.555
3.082ArgPro: 3.082 ± 0.536
3.165ArgGln: 3.165 ± 0.52
7.162ArgArg: 7.162 ± 1.045
3.665ArgSer: 3.665 ± 0.598
4.997ArgThr: 4.997 ± 0.612
6.663ArgVal: 6.663 ± 0.781
1.166ArgTrp: 1.166 ± 0.387
2.249ArgTyr: 2.249 ± 0.351
0.0ArgXaa: 0.0 ± 0.0
Ser
5.997SerAla: 5.997 ± 0.749
0.333SerCys: 0.333 ± 0.163
2.332SerAsp: 2.332 ± 0.36
2.165SerGlu: 2.165 ± 0.449
2.249SerPhe: 2.249 ± 0.452
4.747SerGly: 4.747 ± 0.574
1.333SerHis: 1.333 ± 0.4
3.082SerIle: 3.082 ± 0.548
2.082SerLys: 2.082 ± 0.467
4.248SerLeu: 4.248 ± 0.745
1.582SerMet: 1.582 ± 0.386
1.916SerAsn: 1.916 ± 0.48
2.415SerPro: 2.415 ± 0.401
0.999SerGln: 0.999 ± 0.244
3.665SerArg: 3.665 ± 0.459
3.248SerSer: 3.248 ± 0.47
2.998SerThr: 2.998 ± 0.539
4.497SerVal: 4.497 ± 0.54
0.999SerTrp: 0.999 ± 0.297
1.249SerTyr: 1.249 ± 0.446
0.0SerXaa: 0.0 ± 0.0
Thr
6.746ThrAla: 6.746 ± 0.768
0.75ThrCys: 0.75 ± 0.326
3.498ThrAsp: 3.498 ± 0.592
2.665ThrGlu: 2.665 ± 0.507
2.165ThrPhe: 2.165 ± 0.522
5.247ThrGly: 5.247 ± 0.7
1.166ThrHis: 1.166 ± 0.272
3.165ThrIle: 3.165 ± 0.602
1.916ThrLys: 1.916 ± 0.398
5.33ThrLeu: 5.33 ± 0.778
1.166ThrMet: 1.166 ± 0.282
1.999ThrAsn: 1.999 ± 0.4
3.581ThrPro: 3.581 ± 0.58
2.332ThrGln: 2.332 ± 0.48
4.164ThrArg: 4.164 ± 0.728
3.831ThrSer: 3.831 ± 0.584
3.748ThrThr: 3.748 ± 0.823
4.997ThrVal: 4.997 ± 0.741
0.999ThrTrp: 0.999 ± 0.345
1.416ThrTyr: 1.416 ± 0.357
0.0ThrXaa: 0.0 ± 0.0
Val
7.496ValAla: 7.496 ± 0.855
0.833ValCys: 0.833 ± 0.301
3.998ValAsp: 3.998 ± 0.571
4.164ValGlu: 4.164 ± 0.511
3.082ValPhe: 3.082 ± 0.429
4.997ValGly: 4.997 ± 0.564
0.999ValHis: 0.999 ± 0.294
2.415ValIle: 2.415 ± 0.353
3.498ValLys: 3.498 ± 0.499
6.663ValLeu: 6.663 ± 0.782
1.832ValMet: 1.832 ± 0.5
2.415ValAsn: 2.415 ± 0.499
3.998ValPro: 3.998 ± 0.621
3.665ValGln: 3.665 ± 0.63
6.08ValArg: 6.08 ± 0.707
4.248ValSer: 4.248 ± 0.621
5.164ValThr: 5.164 ± 0.675
5.164ValVal: 5.164 ± 0.616
1.416ValTrp: 1.416 ± 0.358
1.832ValTyr: 1.832 ± 0.347
0.0ValXaa: 0.0 ± 0.0
Trp
1.916TrpAla: 1.916 ± 0.445
0.5TrpCys: 0.5 ± 0.214
0.666TrpAsp: 0.666 ± 0.257
0.5TrpGlu: 0.5 ± 0.148
0.25TrpPhe: 0.25 ± 0.114
0.666TrpGly: 0.666 ± 0.214
0.5TrpHis: 0.5 ± 0.195
0.833TrpIle: 0.833 ± 0.218
0.666TrpLys: 0.666 ± 0.214
1.916TrpLeu: 1.916 ± 0.362
0.5TrpMet: 0.5 ± 0.202
0.75TrpAsn: 0.75 ± 0.293
0.416TrpPro: 0.416 ± 0.169
1.083TrpGln: 1.083 ± 0.366
1.832TrpArg: 1.832 ± 0.366
0.75TrpSer: 0.75 ± 0.234
0.833TrpThr: 0.833 ± 0.223
0.916TrpVal: 0.916 ± 0.324
0.25TrpTrp: 0.25 ± 0.131
0.583TrpTyr: 0.583 ± 0.198
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.748TyrAla: 2.748 ± 0.502
0.416TyrCys: 0.416 ± 0.207
2.082TyrAsp: 2.082 ± 0.428
1.333TyrGlu: 1.333 ± 0.333
1.333TyrPhe: 1.333 ± 0.393
1.999TyrGly: 1.999 ± 0.37
0.75TyrHis: 0.75 ± 0.266
0.999TyrIle: 0.999 ± 0.262
0.5TyrLys: 0.5 ± 0.157
2.332TyrLeu: 2.332 ± 0.656
0.333TyrMet: 0.333 ± 0.157
0.5TyrAsn: 0.5 ± 0.162
1.166TyrPro: 1.166 ± 0.364
1.999TyrGln: 1.999 ± 0.374
2.249TyrArg: 2.249 ± 0.457
0.5TyrSer: 0.5 ± 0.239
1.749TyrThr: 1.749 ± 0.369
1.832TyrVal: 1.832 ± 0.454
0.5TyrTrp: 0.5 ± 0.157
0.666TyrTyr: 0.666 ± 0.22
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (12008 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski