Amino acid dipepetide frequency for Ralstonia phage Dina

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.346AlaAla: 21.346 ± 2.632
1.2AlaCys: 1.2 ± 0.392
9.773AlaAsp: 9.773 ± 0.954
8.744AlaGlu: 8.744 ± 1.158
3.772AlaPhe: 3.772 ± 0.614
10.63AlaGly: 10.63 ± 0.925
2.4AlaHis: 2.4 ± 0.431
5.829AlaIle: 5.829 ± 0.651
5.486AlaLys: 5.486 ± 0.646
12.859AlaLeu: 12.859 ± 1.047
3.772AlaMet: 3.772 ± 0.538
3.772AlaAsn: 3.772 ± 0.568
6.001AlaPro: 6.001 ± 0.969
4.372AlaGln: 4.372 ± 0.67
9.687AlaArg: 9.687 ± 0.968
6.344AlaSer: 6.344 ± 0.628
6.258AlaThr: 6.258 ± 0.737
10.287AlaVal: 10.287 ± 0.956
2.486AlaTrp: 2.486 ± 0.523
3.172AlaTyr: 3.172 ± 0.531
0.0AlaXaa: 0.0 ± 0.0
Cys
0.857CysAla: 0.857 ± 0.339
0.171CysCys: 0.171 ± 0.178
0.257CysAsp: 0.257 ± 0.149
0.857CysGlu: 0.857 ± 0.291
0.086CysPhe: 0.086 ± 0.099
1.2CysGly: 1.2 ± 0.372
0.257CysHis: 0.257 ± 0.147
0.343CysIle: 0.343 ± 0.176
0.6CysLys: 0.6 ± 0.291
0.772CysLeu: 0.772 ± 0.271
0.171CysMet: 0.171 ± 0.129
0.171CysAsn: 0.171 ± 0.109
0.429CysPro: 0.429 ± 0.169
0.0CysGln: 0.0 ± 0.0
0.857CysArg: 0.857 ± 0.256
0.257CysSer: 0.257 ± 0.143
0.514CysThr: 0.514 ± 0.2
0.772CysVal: 0.772 ± 0.315
0.086CysTrp: 0.086 ± 0.101
0.343CysTyr: 0.343 ± 0.159
0.0CysXaa: 0.0 ± 0.0
Asp
8.401AspAla: 8.401 ± 1.095
0.514AspCys: 0.514 ± 0.231
4.372AspAsp: 4.372 ± 0.799
4.115AspGlu: 4.115 ± 0.524
1.715AspPhe: 1.715 ± 0.36
5.829AspGly: 5.829 ± 0.991
1.2AspHis: 1.2 ± 0.342
2.057AspIle: 2.057 ± 0.309
1.715AspLys: 1.715 ± 0.392
4.458AspLeu: 4.458 ± 0.556
1.286AspMet: 1.286 ± 0.243
1.457AspAsn: 1.457 ± 0.288
2.915AspPro: 2.915 ± 0.628
1.8AspGln: 1.8 ± 0.435
3.943AspArg: 3.943 ± 0.623
3.258AspSer: 3.258 ± 0.6
2.572AspThr: 2.572 ± 0.546
3.858AspVal: 3.858 ± 0.563
1.114AspTrp: 1.114 ± 0.25
1.543AspTyr: 1.543 ± 0.412
0.0AspXaa: 0.0 ± 0.0
Glu
7.03GluAla: 7.03 ± 0.905
0.257GluCys: 0.257 ± 0.14
3.086GluAsp: 3.086 ± 0.444
3.258GluGlu: 3.258 ± 0.72
1.8GluPhe: 1.8 ± 0.387
3.515GluGly: 3.515 ± 0.433
1.543GluHis: 1.543 ± 0.351
3.258GluIle: 3.258 ± 0.505
1.629GluLys: 1.629 ± 0.452
7.372GluLeu: 7.372 ± 0.9
0.943GluMet: 0.943 ± 0.28
1.543GluAsn: 1.543 ± 0.389
2.572GluPro: 2.572 ± 0.439
3.943GluGln: 3.943 ± 0.812
5.058GluArg: 5.058 ± 0.931
2.315GluSer: 2.315 ± 0.51
2.572GluThr: 2.572 ± 0.448
4.201GluVal: 4.201 ± 0.68
1.2GluTrp: 1.2 ± 0.273
1.286GluTyr: 1.286 ± 0.265
0.0GluXaa: 0.0 ± 0.0
Phe
3.172PheAla: 3.172 ± 0.467
0.171PheCys: 0.171 ± 0.132
2.829PheAsp: 2.829 ± 0.733
2.057PheGlu: 2.057 ± 0.36
1.2PhePhe: 1.2 ± 0.329
3.0PheGly: 3.0 ± 0.644
0.686PheHis: 0.686 ± 0.208
1.372PheIle: 1.372 ± 0.341
1.2PheLys: 1.2 ± 0.37
2.315PheLeu: 2.315 ± 0.594
0.429PheMet: 0.429 ± 0.224
1.114PheAsn: 1.114 ± 0.355
1.543PhePro: 1.543 ± 0.41
0.6PheGln: 0.6 ± 0.267
1.629PheArg: 1.629 ± 0.417
2.4PheSer: 2.4 ± 0.405
1.286PheThr: 1.286 ± 0.328
2.658PheVal: 2.658 ± 0.453
0.6PheTrp: 0.6 ± 0.196
0.943PheTyr: 0.943 ± 0.225
0.0PheXaa: 0.0 ± 0.0
Gly
9.859GlyAla: 9.859 ± 1.254
0.514GlyCys: 0.514 ± 0.205
4.458GlyAsp: 4.458 ± 0.516
4.629GlyGlu: 4.629 ± 0.791
1.972GlyPhe: 1.972 ± 0.46
8.058GlyGly: 8.058 ± 0.968
1.286GlyHis: 1.286 ± 0.363
3.686GlyIle: 3.686 ± 0.56
2.315GlyLys: 2.315 ± 0.462
7.287GlyLeu: 7.287 ± 0.679
1.543GlyMet: 1.543 ± 0.425
3.0GlyAsn: 3.0 ± 0.516
3.343GlyPro: 3.343 ± 0.534
3.772GlyGln: 3.772 ± 0.613
6.344GlyArg: 6.344 ± 0.633
5.229GlySer: 5.229 ± 0.904
4.372GlyThr: 4.372 ± 0.776
4.801GlyVal: 4.801 ± 0.794
1.886GlyTrp: 1.886 ± 0.48
2.572GlyTyr: 2.572 ± 0.437
0.0GlyXaa: 0.0 ± 0.0
His
2.658HisAla: 2.658 ± 0.522
0.086HisCys: 0.086 ± 0.093
0.772HisAsp: 0.772 ± 0.228
1.029HisGlu: 1.029 ± 0.285
0.429HisPhe: 0.429 ± 0.194
1.886HisGly: 1.886 ± 0.395
0.514HisHis: 0.514 ± 0.251
0.943HisIle: 0.943 ± 0.268
0.686HisLys: 0.686 ± 0.205
1.457HisLeu: 1.457 ± 0.384
0.429HisMet: 0.429 ± 0.177
0.171HisAsn: 0.171 ± 0.121
1.972HisPro: 1.972 ± 0.564
0.772HisGln: 0.772 ± 0.203
1.629HisArg: 1.629 ± 0.363
0.686HisSer: 0.686 ± 0.227
1.029HisThr: 1.029 ± 0.265
1.715HisVal: 1.715 ± 0.342
0.429HisTrp: 0.429 ± 0.244
1.029HisTyr: 1.029 ± 0.304
0.0HisXaa: 0.0 ± 0.0
Ile
5.744IleAla: 5.744 ± 0.755
0.514IleCys: 0.514 ± 0.22
3.515IleAsp: 3.515 ± 0.481
3.686IleGlu: 3.686 ± 0.549
0.857IlePhe: 0.857 ± 0.227
3.258IleGly: 3.258 ± 0.56
0.514IleHis: 0.514 ± 0.211
0.686IleIle: 0.686 ± 0.297
1.286IleLys: 1.286 ± 0.34
2.486IleLeu: 2.486 ± 0.462
0.514IleMet: 0.514 ± 0.229
1.543IleAsn: 1.543 ± 0.391
1.629IlePro: 1.629 ± 0.383
1.715IleGln: 1.715 ± 0.384
3.258IleArg: 3.258 ± 0.487
2.486IleSer: 2.486 ± 0.503
2.915IleThr: 2.915 ± 0.604
2.143IleVal: 2.143 ± 0.387
0.772IleTrp: 0.772 ± 0.295
1.372IleTyr: 1.372 ± 0.318
0.0IleXaa: 0.0 ± 0.0
Lys
4.886LysAla: 4.886 ± 0.858
0.343LysCys: 0.343 ± 0.211
0.6LysAsp: 0.6 ± 0.334
1.114LysGlu: 1.114 ± 0.379
1.2LysPhe: 1.2 ± 0.273
2.915LysGly: 2.915 ± 0.57
0.514LysHis: 0.514 ± 0.21
1.029LysIle: 1.029 ± 0.319
2.143LysLys: 2.143 ± 0.616
2.743LysLeu: 2.743 ± 0.532
0.086LysMet: 0.086 ± 0.096
0.857LysAsn: 0.857 ± 0.268
1.972LysPro: 1.972 ± 0.392
1.2LysGln: 1.2 ± 0.328
3.343LysArg: 3.343 ± 0.731
2.057LysSer: 2.057 ± 0.625
2.658LysThr: 2.658 ± 0.623
2.229LysVal: 2.229 ± 0.422
0.772LysTrp: 0.772 ± 0.261
0.6LysTyr: 0.6 ± 0.222
0.0LysXaa: 0.0 ± 0.0
Leu
13.288LeuAla: 13.288 ± 1.209
0.943LeuCys: 0.943 ± 0.319
4.972LeuAsp: 4.972 ± 0.546
4.972LeuGlu: 4.972 ± 0.626
3.086LeuPhe: 3.086 ± 0.551
6.001LeuGly: 6.001 ± 0.634
2.4LeuHis: 2.4 ± 0.547
3.086LeuIle: 3.086 ± 0.482
2.915LeuLys: 2.915 ± 0.63
6.772LeuLeu: 6.772 ± 0.825
1.029LeuMet: 1.029 ± 0.272
1.972LeuAsn: 1.972 ± 0.35
4.715LeuPro: 4.715 ± 0.708
4.286LeuGln: 4.286 ± 0.571
6.944LeuArg: 6.944 ± 0.93
5.315LeuSer: 5.315 ± 0.492
5.401LeuThr: 5.401 ± 0.606
6.172LeuVal: 6.172 ± 0.744
1.543LeuTrp: 1.543 ± 0.369
2.229LeuTyr: 2.229 ± 0.403
0.0LeuXaa: 0.0 ± 0.0
Met
2.057MetAla: 2.057 ± 0.374
0.171MetCys: 0.171 ± 0.173
0.857MetAsp: 0.857 ± 0.245
0.857MetGlu: 0.857 ± 0.351
0.772MetPhe: 0.772 ± 0.255
1.286MetGly: 1.286 ± 0.325
0.514MetHis: 0.514 ± 0.218
0.772MetIle: 0.772 ± 0.35
1.029MetLys: 1.029 ± 0.257
1.543MetLeu: 1.543 ± 0.38
0.514MetMet: 0.514 ± 0.223
0.686MetAsn: 0.686 ± 0.257
1.8MetPro: 1.8 ± 0.423
1.2MetGln: 1.2 ± 0.375
1.972MetArg: 1.972 ± 0.419
0.772MetSer: 0.772 ± 0.243
2.057MetThr: 2.057 ± 0.406
0.857MetVal: 0.857 ± 0.254
0.086MetTrp: 0.086 ± 0.088
0.429MetTyr: 0.429 ± 0.204
0.0MetXaa: 0.0 ± 0.0
Asn
3.429AsnAla: 3.429 ± 0.509
0.171AsnCys: 0.171 ± 0.118
1.372AsnAsp: 1.372 ± 0.392
1.629AsnGlu: 1.629 ± 0.399
1.114AsnPhe: 1.114 ± 0.371
3.601AsnGly: 3.601 ± 0.616
0.686AsnHis: 0.686 ± 0.248
1.457AsnIle: 1.457 ± 0.285
0.514AsnLys: 0.514 ± 0.219
2.143AsnLeu: 2.143 ± 0.474
0.6AsnMet: 0.6 ± 0.221
0.429AsnAsn: 0.429 ± 0.195
2.315AsnPro: 2.315 ± 0.572
0.772AsnGln: 0.772 ± 0.235
1.886AsnArg: 1.886 ± 0.495
0.686AsnSer: 0.686 ± 0.263
1.972AsnThr: 1.972 ± 0.378
2.229AsnVal: 2.229 ± 0.522
0.343AsnTrp: 0.343 ± 0.177
0.514AsnTyr: 0.514 ± 0.208
0.0AsnXaa: 0.0 ± 0.0
Pro
8.401ProAla: 8.401 ± 0.832
0.343ProCys: 0.343 ± 0.176
3.086ProAsp: 3.086 ± 0.503
3.601ProGlu: 3.601 ± 0.609
0.943ProPhe: 0.943 ± 0.332
4.544ProGly: 4.544 ± 0.732
1.2ProHis: 1.2 ± 0.359
1.543ProIle: 1.543 ± 0.345
1.629ProLys: 1.629 ± 0.427
4.029ProLeu: 4.029 ± 0.652
1.372ProMet: 1.372 ± 0.248
1.629ProAsn: 1.629 ± 0.389
3.515ProPro: 3.515 ± 0.677
1.543ProGln: 1.543 ± 0.361
3.858ProArg: 3.858 ± 0.523
3.343ProSer: 3.343 ± 0.47
2.915ProThr: 2.915 ± 0.492
2.915ProVal: 2.915 ± 0.585
1.029ProTrp: 1.029 ± 0.264
1.629ProTyr: 1.629 ± 0.444
0.0ProXaa: 0.0 ± 0.0
Gln
4.886GlnAla: 4.886 ± 0.516
0.086GlnCys: 0.086 ± 0.089
1.972GlnAsp: 1.972 ± 0.421
1.543GlnGlu: 1.543 ± 0.36
1.715GlnPhe: 1.715 ± 0.351
2.486GlnGly: 2.486 ± 0.399
0.943GlnHis: 0.943 ± 0.297
2.658GlnIle: 2.658 ± 0.434
1.457GlnLys: 1.457 ± 0.368
5.229GlnLeu: 5.229 ± 0.856
1.029GlnMet: 1.029 ± 0.306
0.857GlnAsn: 0.857 ± 0.259
1.886GlnPro: 1.886 ± 0.324
2.829GlnGln: 2.829 ± 0.732
3.0GlnArg: 3.0 ± 0.638
2.229GlnSer: 2.229 ± 0.459
2.4GlnThr: 2.4 ± 0.394
2.829GlnVal: 2.829 ± 0.48
0.514GlnTrp: 0.514 ± 0.204
0.772GlnTyr: 0.772 ± 0.222
0.0GlnXaa: 0.0 ± 0.0
Arg
10.973ArgAla: 10.973 ± 1.322
0.686ArgCys: 0.686 ± 0.195
3.086ArgAsp: 3.086 ± 0.558
4.201ArgGlu: 4.201 ± 0.73
2.915ArgPhe: 2.915 ± 0.592
5.058ArgGly: 5.058 ± 0.629
1.629ArgHis: 1.629 ± 0.442
3.858ArgIle: 3.858 ± 0.57
2.658ArgLys: 2.658 ± 0.445
6.772ArgLeu: 6.772 ± 0.718
1.457ArgMet: 1.457 ± 0.333
2.572ArgAsn: 2.572 ± 0.383
3.858ArgPro: 3.858 ± 0.707
3.601ArgGln: 3.601 ± 0.695
7.287ArgArg: 7.287 ± 0.999
4.201ArgSer: 4.201 ± 0.633
3.772ArgThr: 3.772 ± 0.542
5.572ArgVal: 5.572 ± 0.89
1.543ArgTrp: 1.543 ± 0.433
2.315ArgTyr: 2.315 ± 0.417
0.0ArgXaa: 0.0 ± 0.0
Ser
7.63SerAla: 7.63 ± 1.191
0.857SerCys: 0.857 ± 0.229
2.572SerAsp: 2.572 ± 0.442
3.0SerGlu: 3.0 ± 0.492
1.2SerPhe: 1.2 ± 0.402
3.943SerGly: 3.943 ± 0.781
1.029SerHis: 1.029 ± 0.292
2.829SerIle: 2.829 ± 0.496
1.972SerLys: 1.972 ± 0.402
4.801SerLeu: 4.801 ± 0.541
1.372SerMet: 1.372 ± 0.384
1.8SerAsn: 1.8 ± 0.359
2.743SerPro: 2.743 ± 0.578
2.143SerGln: 2.143 ± 0.435
3.086SerArg: 3.086 ± 0.384
3.858SerSer: 3.858 ± 0.473
2.658SerThr: 2.658 ± 0.356
3.943SerVal: 3.943 ± 0.8
0.686SerTrp: 0.686 ± 0.225
1.372SerTyr: 1.372 ± 0.303
0.0SerXaa: 0.0 ± 0.0
Thr
6.858ThrAla: 6.858 ± 0.878
0.6ThrCys: 0.6 ± 0.226
3.258ThrAsp: 3.258 ± 0.544
2.658ThrGlu: 2.658 ± 0.389
1.972ThrPhe: 1.972 ± 0.443
4.972ThrGly: 4.972 ± 0.594
0.943ThrHis: 0.943 ± 0.293
2.4ThrIle: 2.4 ± 0.458
0.943ThrLys: 0.943 ± 0.222
4.115ThrLeu: 4.115 ± 0.553
1.114ThrMet: 1.114 ± 0.269
1.114ThrAsn: 1.114 ± 0.355
4.458ThrPro: 4.458 ± 0.68
2.743ThrGln: 2.743 ± 0.493
3.686ThrArg: 3.686 ± 0.621
2.658ThrSer: 2.658 ± 0.469
3.429ThrThr: 3.429 ± 0.778
4.029ThrVal: 4.029 ± 0.666
1.114ThrTrp: 1.114 ± 0.338
1.715ThrTyr: 1.715 ± 0.433
0.0ThrXaa: 0.0 ± 0.0
Val
10.287ValAla: 10.287 ± 1.087
1.029ValCys: 1.029 ± 0.289
4.629ValAsp: 4.629 ± 0.851
4.201ValGlu: 4.201 ± 0.697
3.086ValPhe: 3.086 ± 0.52
5.744ValGly: 5.744 ± 0.699
1.114ValHis: 1.114 ± 0.319
2.143ValIle: 2.143 ± 0.384
1.972ValLys: 1.972 ± 0.533
6.772ValLeu: 6.772 ± 0.734
1.286ValMet: 1.286 ± 0.391
1.457ValAsn: 1.457 ± 0.289
3.429ValPro: 3.429 ± 0.572
2.057ValGln: 2.057 ± 0.387
5.915ValArg: 5.915 ± 0.596
2.743ValSer: 2.743 ± 0.498
3.172ValThr: 3.172 ± 0.589
4.629ValVal: 4.629 ± 0.763
1.029ValTrp: 1.029 ± 0.323
1.715ValTyr: 1.715 ± 0.434
0.0ValXaa: 0.0 ± 0.0
Trp
2.4TrpAla: 2.4 ± 0.558
0.171TrpCys: 0.171 ± 0.119
0.943TrpAsp: 0.943 ± 0.257
1.029TrpGlu: 1.029 ± 0.322
0.6TrpPhe: 0.6 ± 0.233
0.343TrpGly: 0.343 ± 0.165
0.772TrpHis: 0.772 ± 0.344
0.6TrpIle: 0.6 ± 0.286
0.429TrpLys: 0.429 ± 0.174
1.8TrpLeu: 1.8 ± 0.384
0.6TrpMet: 0.6 ± 0.2
0.772TrpAsn: 0.772 ± 0.202
0.686TrpPro: 0.686 ± 0.255
1.286TrpGln: 1.286 ± 0.314
2.143TrpArg: 2.143 ± 0.483
0.857TrpSer: 0.857 ± 0.269
0.686TrpThr: 0.686 ± 0.235
1.286TrpVal: 1.286 ± 0.309
0.6TrpTrp: 0.6 ± 0.196
0.257TrpTyr: 0.257 ± 0.142
0.0TrpXaa: 0.0 ± 0.0
Tyr
4.029TyrAla: 4.029 ± 0.448
0.171TyrCys: 0.171 ± 0.115
1.715TyrAsp: 1.715 ± 0.448
1.2TyrGlu: 1.2 ± 0.325
0.772TyrPhe: 0.772 ± 0.246
2.658TyrGly: 2.658 ± 0.47
0.171TyrHis: 0.171 ± 0.106
0.343TyrIle: 0.343 ± 0.167
0.686TyrLys: 0.686 ± 0.284
2.315TyrLeu: 2.315 ± 0.397
0.6TyrMet: 0.6 ± 0.226
0.943TyrAsn: 0.943 ± 0.275
1.286TyrPro: 1.286 ± 0.29
0.686TyrGln: 0.686 ± 0.228
2.486TyrArg: 2.486 ± 0.431
1.886TyrSer: 1.886 ± 0.476
2.143TyrThr: 2.143 ± 0.414
1.286TyrVal: 1.286 ± 0.303
0.429TyrTrp: 0.429 ± 0.188
0.429TyrTyr: 0.429 ± 0.215
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (11666 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski