Enterobacter phage EcP1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Eceepunavirus; Enterobacter virus EcP1

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 77 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E9NIF4|E9NIF4_9CAUD Uncharacterized protein OS=Enterobacter phage EcP1 OX=942016 GN=EcP1_gp29 PE=4 SV=1
MM1 pKa = 7.41GCDD4 pKa = 2.93IHH6 pKa = 8.35CYY8 pKa = 8.22SEE10 pKa = 3.99KK11 pKa = 10.45RR12 pKa = 11.84NKK14 pKa = 8.32EE15 pKa = 3.9TGKK18 pKa = 9.88WEE20 pKa = 5.45LIPEE24 pKa = 4.58MIKK27 pKa = 10.64EE28 pKa = 3.81IDD30 pKa = 3.55GDD32 pKa = 3.94YY33 pKa = 11.03TDD35 pKa = 4.2YY36 pKa = 10.43QTDD39 pKa = 3.59QILSNRR45 pKa = 11.84NYY47 pKa = 9.88MLFSILADD55 pKa = 3.26VRR57 pKa = 11.84NYY59 pKa = 10.65GLNAYY64 pKa = 9.64CIISEE69 pKa = 4.16PKK71 pKa = 10.28GLPEE75 pKa = 4.74DD76 pKa = 3.69VSNEE80 pKa = 3.86VKK82 pKa = 10.61EE83 pKa = 4.26MSDD86 pKa = 3.18YY87 pKa = 10.5WDD89 pKa = 4.41CDD91 pKa = 3.36GHH93 pKa = 5.08SHH95 pKa = 6.11SWHH98 pKa = 5.53TLKK101 pKa = 11.0DD102 pKa = 3.98LLDD105 pKa = 3.97HH106 pKa = 6.33SAKK109 pKa = 10.22FKK111 pKa = 10.84NEE113 pKa = 3.36RR114 pKa = 11.84RR115 pKa = 11.84LLEE118 pKa = 4.41IFPEE122 pKa = 4.31DD123 pKa = 4.02LTEE126 pKa = 3.83EE127 pKa = 4.35WYY129 pKa = 11.1LNQINEE135 pKa = 5.01CIQQADD141 pKa = 3.95EE142 pKa = 5.02LNIEE146 pKa = 4.2PEE148 pKa = 3.85NYY150 pKa = 10.11RR151 pKa = 11.84IVFWFDD157 pKa = 2.62NN158 pKa = 3.84

Molecular weight: 18.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E9NID7|E9NID7_9CAUD Uncharacterized protein OS=Enterobacter phage EcP1 OX=942016 GN=EcP1_gp12 PE=4 SV=1
MM1 pKa = 7.88CDD3 pKa = 3.02CSKK6 pKa = 10.72IVLEE10 pKa = 4.22KK11 pKa = 10.77VVNKK15 pKa = 9.38MKK17 pKa = 10.96SEE19 pKa = 4.0LTKK22 pKa = 11.0GSVVTVNPYY31 pKa = 8.35WDD33 pKa = 3.64SMSLVSINGVFTSRR47 pKa = 11.84LSLKK51 pKa = 10.25ISGRR55 pKa = 11.84YY56 pKa = 8.94RR57 pKa = 11.84KK58 pKa = 9.48GKK60 pKa = 10.05KK61 pKa = 9.83DD62 pKa = 3.13GSTSITEE69 pKa = 4.34SKK71 pKa = 10.78LDD73 pKa = 3.69TAVLPSYY80 pKa = 11.12CPFCGVKK87 pKa = 10.17YY88 pKa = 10.3PKK90 pKa = 10.55

Molecular weight: 9.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

77

0

77

18764

25

3502

243.7

27.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.269 ± 0.443

1.124 ± 0.225

6.081 ± 0.133

7.035 ± 0.193

3.795 ± 0.145

5.788 ± 0.247

1.689 ± 0.154

6.886 ± 0.249

7.76 ± 0.204

8.106 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.995 ± 0.134

6.326 ± 0.334

3.693 ± 0.139

3.912 ± 0.216

3.725 ± 0.154

6.656 ± 0.22

5.894 ± 0.21

6.369 ± 0.248

1.002 ± 0.112

3.896 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski