Amino acid dipepetide frequency for Enterobacter phage EcP1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.182AlaAla: 6.182 ± 0.661
0.853AlaCys: 0.853 ± 0.275
3.891AlaAsp: 3.891 ± 0.467
5.543AlaGlu: 5.543 ± 0.632
2.345AlaPhe: 2.345 ± 0.317
4.53AlaGly: 4.53 ± 0.676
1.759AlaHis: 1.759 ± 0.35
4.957AlaIle: 4.957 ± 0.509
5.863AlaLys: 5.863 ± 0.661
5.49AlaLeu: 5.49 ± 0.594
2.345AlaMet: 2.345 ± 0.401
3.944AlaAsn: 3.944 ± 0.745
2.185AlaPro: 2.185 ± 0.557
3.091AlaGln: 3.091 ± 0.41
2.771AlaArg: 2.771 ± 0.422
3.837AlaSer: 3.837 ± 0.591
4.69AlaThr: 4.69 ± 0.666
4.85AlaVal: 4.85 ± 0.767
0.959AlaTrp: 0.959 ± 0.228
2.612AlaTyr: 2.612 ± 0.282
0.0AlaXaa: 0.0 ± 0.0
Cys
0.533CysAla: 0.533 ± 0.239
0.266CysCys: 0.266 ± 0.166
0.906CysAsp: 0.906 ± 0.283
0.693CysGlu: 0.693 ± 0.242
0.48CysPhe: 0.48 ± 0.165
0.746CysGly: 0.746 ± 0.332
0.16CysHis: 0.16 ± 0.1
0.906CysIle: 0.906 ± 0.312
0.373CysLys: 0.373 ± 0.153
0.746CysLeu: 0.746 ± 0.256
0.213CysMet: 0.213 ± 0.125
0.586CysAsn: 0.586 ± 0.212
0.693CysPro: 0.693 ± 0.299
0.107CysGln: 0.107 ± 0.081
0.533CysArg: 0.533 ± 0.2
0.959CysSer: 0.959 ± 0.278
0.64CysThr: 0.64 ± 0.204
0.906CysVal: 0.906 ± 0.304
0.16CysTrp: 0.16 ± 0.101
0.64CysTyr: 0.64 ± 0.228
0.0CysXaa: 0.0 ± 0.0
Asp
5.01AspAla: 5.01 ± 0.902
0.586AspCys: 0.586 ± 0.223
3.251AspAsp: 3.251 ± 0.551
3.891AspGlu: 3.891 ± 0.713
2.558AspPhe: 2.558 ± 0.378
3.837AspGly: 3.837 ± 0.466
1.332AspHis: 1.332 ± 0.248
5.33AspIle: 5.33 ± 0.554
4.743AspLys: 4.743 ± 0.484
4.903AspLeu: 4.903 ± 0.503
2.185AspMet: 2.185 ± 0.273
3.464AspAsn: 3.464 ± 0.397
1.919AspPro: 1.919 ± 0.256
1.652AspGln: 1.652 ± 0.302
2.025AspArg: 2.025 ± 0.325
4.317AspSer: 4.317 ± 0.372
3.624AspThr: 3.624 ± 0.471
3.411AspVal: 3.411 ± 0.355
0.693AspTrp: 0.693 ± 0.169
2.079AspTyr: 2.079 ± 0.419
0.0AspXaa: 0.0 ± 0.0
Glu
5.223GluAla: 5.223 ± 0.571
0.693GluCys: 0.693 ± 0.248
3.784GluAsp: 3.784 ± 0.431
5.49GluGlu: 5.49 ± 0.774
2.612GluPhe: 2.612 ± 0.373
3.624GluGly: 3.624 ± 0.412
1.546GluHis: 1.546 ± 0.241
4.85GluIle: 4.85 ± 0.589
5.596GluLys: 5.596 ± 0.484
6.236GluLeu: 6.236 ± 0.552
2.345GluMet: 2.345 ± 0.353
3.518GluAsn: 3.518 ± 0.481
2.985GluPro: 2.985 ± 0.456
2.718GluGln: 2.718 ± 0.417
2.931GluArg: 2.931 ± 0.446
4.583GluSer: 4.583 ± 0.374
3.784GluThr: 3.784 ± 0.399
4.53GluVal: 4.53 ± 0.556
0.586GluTrp: 0.586 ± 0.244
2.718GluTyr: 2.718 ± 0.273
0.0GluXaa: 0.0 ± 0.0
Phe
2.238PheAla: 2.238 ± 0.289
0.48PheCys: 0.48 ± 0.169
2.878PheAsp: 2.878 ± 0.374
2.505PheGlu: 2.505 ± 0.287
1.492PhePhe: 1.492 ± 0.252
2.931PheGly: 2.931 ± 0.465
0.426PheHis: 0.426 ± 0.221
1.919PheIle: 1.919 ± 0.309
3.091PheLys: 3.091 ± 0.433
2.345PheLeu: 2.345 ± 0.352
0.906PheMet: 0.906 ± 0.249
2.878PheAsn: 2.878 ± 0.337
1.226PhePro: 1.226 ± 0.366
1.013PheGln: 1.013 ± 0.208
1.332PheArg: 1.332 ± 0.253
2.718PheSer: 2.718 ± 0.346
2.718PheThr: 2.718 ± 0.374
2.825PheVal: 2.825 ± 0.371
0.213PheTrp: 0.213 ± 0.113
1.812PheTyr: 1.812 ± 0.359
0.0PheXaa: 0.0 ± 0.0
Gly
4.051GlyAla: 4.051 ± 0.569
0.586GlyCys: 0.586 ± 0.216
2.931GlyAsp: 2.931 ± 0.516
3.464GlyGlu: 3.464 ± 0.384
2.505GlyPhe: 2.505 ± 0.394
2.825GlyGly: 2.825 ± 0.589
0.799GlyHis: 0.799 ± 0.216
5.01GlyIle: 5.01 ± 0.489
4.85GlyLys: 4.85 ± 0.427
4.903GlyLeu: 4.903 ± 0.734
1.972GlyMet: 1.972 ± 0.335
3.731GlyAsn: 3.731 ± 0.38
0.107GlyPro: 0.107 ± 0.06
2.132GlyGln: 2.132 ± 0.403
1.599GlyArg: 1.599 ± 0.287
5.01GlySer: 5.01 ± 0.539
5.01GlyThr: 5.01 ± 0.649
3.358GlyVal: 3.358 ± 0.525
0.746GlyTrp: 0.746 ± 0.203
2.292GlyTyr: 2.292 ± 0.29
0.0GlyXaa: 0.0 ± 0.0
His
0.959HisAla: 0.959 ± 0.242
0.266HisCys: 0.266 ± 0.124
1.332HisAsp: 1.332 ± 0.345
1.332HisGlu: 1.332 ± 0.327
0.693HisPhe: 0.693 ± 0.19
0.586HisGly: 0.586 ± 0.153
0.16HisHis: 0.16 ± 0.104
1.546HisIle: 1.546 ± 0.301
1.173HisLys: 1.173 ± 0.402
1.066HisLeu: 1.066 ± 0.277
0.693HisMet: 0.693 ± 0.155
1.066HisAsn: 1.066 ± 0.314
0.48HisPro: 0.48 ± 0.134
0.533HisGln: 0.533 ± 0.185
0.746HisArg: 0.746 ± 0.238
1.066HisSer: 1.066 ± 0.308
1.226HisThr: 1.226 ± 0.27
1.119HisVal: 1.119 ± 0.259
0.213HisTrp: 0.213 ± 0.115
0.64HisTyr: 0.64 ± 0.232
0.0HisXaa: 0.0 ± 0.0
Ile
4.85IleAla: 4.85 ± 0.542
1.066IleCys: 1.066 ± 0.326
4.85IleAsp: 4.85 ± 0.466
4.797IleGlu: 4.797 ± 0.505
2.185IlePhe: 2.185 ± 0.332
4.051IleGly: 4.051 ± 0.622
1.439IleHis: 1.439 ± 0.259
4.797IleIle: 4.797 ± 0.54
5.916IleLys: 5.916 ± 0.591
4.104IleLeu: 4.104 ± 0.379
1.919IleMet: 1.919 ± 0.342
6.129IleAsn: 6.129 ± 0.682
2.771IlePro: 2.771 ± 0.338
2.718IleGln: 2.718 ± 0.35
2.665IleArg: 2.665 ± 0.364
4.85IleSer: 4.85 ± 0.692
3.624IleThr: 3.624 ± 0.434
3.464IleVal: 3.464 ± 0.517
0.16IleTrp: 0.16 ± 0.114
2.505IleTyr: 2.505 ± 0.339
0.0IleXaa: 0.0 ± 0.0
Lys
5.596LysAla: 5.596 ± 0.563
0.853LysCys: 0.853 ± 0.247
4.583LysAsp: 4.583 ± 0.562
6.502LysGlu: 6.502 ± 0.6
2.878LysPhe: 2.878 ± 0.306
3.837LysGly: 3.837 ± 0.42
1.599LysHis: 1.599 ± 0.375
4.104LysIle: 4.104 ± 0.437
5.223LysLys: 5.223 ± 0.432
7.355LysLeu: 7.355 ± 0.695
2.665LysMet: 2.665 ± 0.424
3.944LysAsn: 3.944 ± 0.361
2.718LysPro: 2.718 ± 0.543
3.251LysGln: 3.251 ± 0.406
3.091LysArg: 3.091 ± 0.376
5.276LysSer: 5.276 ± 0.586
4.85LysThr: 4.85 ± 0.433
5.33LysVal: 5.33 ± 0.429
1.119LysTrp: 1.119 ± 0.244
2.825LysTyr: 2.825 ± 0.408
0.0LysXaa: 0.0 ± 0.0
Leu
6.076LeuAla: 6.076 ± 0.673
0.959LeuCys: 0.959 ± 0.299
4.53LeuAsp: 4.53 ± 0.526
4.157LeuGlu: 4.157 ± 0.404
3.251LeuPhe: 3.251 ± 0.533
5.01LeuGly: 5.01 ± 0.952
1.226LeuHis: 1.226 ± 0.27
5.543LeuIle: 5.543 ± 0.758
6.342LeuLys: 6.342 ± 0.543
6.289LeuLeu: 6.289 ± 0.752
2.132LeuMet: 2.132 ± 0.298
6.236LeuAsn: 6.236 ± 0.527
3.997LeuPro: 3.997 ± 0.505
2.505LeuGln: 2.505 ± 0.234
3.411LeuArg: 3.411 ± 0.467
6.182LeuSer: 6.182 ± 0.581
4.69LeuThr: 4.69 ± 0.631
5.383LeuVal: 5.383 ± 0.518
0.799LeuTrp: 0.799 ± 0.231
2.345LeuTyr: 2.345 ± 0.378
0.0LeuXaa: 0.0 ± 0.0
Met
2.132MetAla: 2.132 ± 0.356
0.266MetCys: 0.266 ± 0.132
1.865MetAsp: 1.865 ± 0.276
1.546MetGlu: 1.546 ± 0.338
1.119MetPhe: 1.119 ± 0.208
1.865MetGly: 1.865 ± 0.243
0.586MetHis: 0.586 ± 0.171
1.332MetIle: 1.332 ± 0.363
2.025MetLys: 2.025 ± 0.292
2.771MetLeu: 2.771 ± 0.395
0.799MetMet: 0.799 ± 0.228
2.612MetAsn: 2.612 ± 0.333
1.226MetPro: 1.226 ± 0.245
2.025MetGln: 2.025 ± 0.346
0.799MetArg: 0.799 ± 0.254
2.505MetSer: 2.505 ± 0.349
1.705MetThr: 1.705 ± 0.241
1.599MetVal: 1.599 ± 0.299
0.266MetTrp: 0.266 ± 0.12
0.906MetTyr: 0.906 ± 0.289
0.0MetXaa: 0.0 ± 0.0
Asn
4.37AsnAla: 4.37 ± 0.711
0.906AsnCys: 0.906 ± 0.296
2.825AsnAsp: 2.825 ± 0.305
3.997AsnGlu: 3.997 ± 0.47
2.185AsnPhe: 2.185 ± 0.335
4.104AsnGly: 4.104 ± 0.435
0.746AsnHis: 0.746 ± 0.187
4.424AsnIle: 4.424 ± 0.512
5.116AsnLys: 5.116 ± 0.443
4.957AsnLeu: 4.957 ± 0.621
1.919AsnMet: 1.919 ± 0.296
3.891AsnAsn: 3.891 ± 0.428
3.518AsnPro: 3.518 ± 0.472
3.251AsnGln: 3.251 ± 0.527
2.505AsnArg: 2.505 ± 0.472
3.677AsnSer: 3.677 ± 0.544
3.784AsnThr: 3.784 ± 0.4
3.251AsnVal: 3.251 ± 0.55
0.48AsnTrp: 0.48 ± 0.173
3.358AsnTyr: 3.358 ± 0.49
0.0AsnXaa: 0.0 ± 0.0
Pro
3.358ProAla: 3.358 ± 0.398
0.48ProCys: 0.48 ± 0.188
2.612ProAsp: 2.612 ± 0.459
4.051ProGlu: 4.051 ± 0.674
1.439ProPhe: 1.439 ± 0.303
0.746ProGly: 0.746 ± 0.211
0.266ProHis: 0.266 ± 0.116
2.292ProIle: 2.292 ± 0.32
2.718ProLys: 2.718 ± 0.363
2.985ProLeu: 2.985 ± 0.414
1.013ProMet: 1.013 ± 0.241
2.132ProAsn: 2.132 ± 0.398
1.226ProPro: 1.226 ± 0.336
1.546ProGln: 1.546 ± 0.252
0.906ProArg: 0.906 ± 0.228
2.238ProSer: 2.238 ± 0.363
2.398ProThr: 2.398 ± 0.299
2.771ProVal: 2.771 ± 0.501
0.32ProTrp: 0.32 ± 0.156
1.439ProTyr: 1.439 ± 0.252
0.0ProXaa: 0.0 ± 0.0
Gln
2.931GlnAla: 2.931 ± 0.634
0.266GlnCys: 0.266 ± 0.15
1.386GlnAsp: 1.386 ± 0.29
3.091GlnGlu: 3.091 ± 0.392
1.119GlnPhe: 1.119 ± 0.251
2.238GlnGly: 2.238 ± 0.324
0.693GlnHis: 0.693 ± 0.168
2.612GlnIle: 2.612 ± 0.453
2.878GlnLys: 2.878 ± 0.378
3.464GlnLeu: 3.464 ± 0.444
1.279GlnMet: 1.279 ± 0.22
1.652GlnAsn: 1.652 ± 0.403
1.599GlnPro: 1.599 ± 0.324
2.238GlnGln: 2.238 ± 0.452
1.546GlnArg: 1.546 ± 0.307
2.665GlnSer: 2.665 ± 0.339
2.558GlnThr: 2.558 ± 0.391
1.865GlnVal: 1.865 ± 0.324
0.373GlnTrp: 0.373 ± 0.13
2.665GlnTyr: 2.665 ± 0.434
0.0GlnXaa: 0.0 ± 0.0
Arg
2.292ArgAla: 2.292 ± 0.428
0.533ArgCys: 0.533 ± 0.266
2.612ArgAsp: 2.612 ± 0.385
3.038ArgGlu: 3.038 ± 0.418
1.279ArgPhe: 1.279 ± 0.283
1.652ArgGly: 1.652 ± 0.322
0.426ArgHis: 0.426 ± 0.179
2.558ArgIle: 2.558 ± 0.359
3.358ArgLys: 3.358 ± 0.419
3.624ArgLeu: 3.624 ± 0.294
1.173ArgMet: 1.173 ± 0.228
2.718ArgAsn: 2.718 ± 0.442
0.853ArgPro: 0.853 ± 0.273
1.119ArgGln: 1.119 ± 0.228
1.119ArgArg: 1.119 ± 0.244
2.292ArgSer: 2.292 ± 0.348
1.919ArgThr: 1.919 ± 0.291
2.452ArgVal: 2.452 ± 0.384
0.533ArgTrp: 0.533 ± 0.163
1.705ArgTyr: 1.705 ± 0.418
0.0ArgXaa: 0.0 ± 0.0
Ser
4.37SerAla: 4.37 ± 0.438
0.426SerCys: 0.426 ± 0.226
4.583SerAsp: 4.583 ± 0.49
4.69SerGlu: 4.69 ± 0.75
2.452SerPhe: 2.452 ± 0.342
5.223SerGly: 5.223 ± 0.516
1.013SerHis: 1.013 ± 0.276
4.637SerIle: 4.637 ± 0.619
5.223SerLys: 5.223 ± 0.454
6.555SerLeu: 6.555 ± 0.556
1.919SerMet: 1.919 ± 0.436
3.891SerAsn: 3.891 ± 0.492
2.505SerPro: 2.505 ± 0.311
2.398SerGln: 2.398 ± 0.34
2.558SerArg: 2.558 ± 0.323
3.571SerSer: 3.571 ± 0.417
3.198SerThr: 3.198 ± 0.572
4.424SerVal: 4.424 ± 0.428
0.533SerTrp: 0.533 ± 0.164
2.345SerTyr: 2.345 ± 0.416
0.0SerXaa: 0.0 ± 0.0
Thr
4.583ThrAla: 4.583 ± 0.576
0.373ThrCys: 0.373 ± 0.191
5.01ThrAsp: 5.01 ± 0.522
4.157ThrGlu: 4.157 ± 0.514
2.718ThrPhe: 2.718 ± 0.356
3.731ThrGly: 3.731 ± 0.485
0.64ThrHis: 0.64 ± 0.159
3.997ThrIle: 3.997 ± 0.624
5.756ThrLys: 5.756 ± 0.682
3.944ThrLeu: 3.944 ± 0.422
0.853ThrMet: 0.853 ± 0.18
3.251ThrAsn: 3.251 ± 0.565
2.665ThrPro: 2.665 ± 0.419
2.185ThrGln: 2.185 ± 0.297
2.132ThrArg: 2.132 ± 0.347
2.825ThrSer: 2.825 ± 0.553
2.931ThrThr: 2.931 ± 0.656
4.69ThrVal: 4.69 ± 0.553
0.746ThrTrp: 0.746 ± 0.235
1.759ThrTyr: 1.759 ± 0.322
0.0ThrXaa: 0.0 ± 0.0
Val
4.477ValAla: 4.477 ± 0.615
0.533ValCys: 0.533 ± 0.185
4.424ValAsp: 4.424 ± 0.531
4.157ValGlu: 4.157 ± 0.402
2.558ValPhe: 2.558 ± 0.314
3.411ValGly: 3.411 ± 0.471
1.013ValHis: 1.013 ± 0.24
4.317ValIle: 4.317 ± 0.451
4.157ValLys: 4.157 ± 0.484
4.903ValLeu: 4.903 ± 0.571
2.079ValMet: 2.079 ± 0.358
4.37ValAsn: 4.37 ± 0.668
2.825ValPro: 2.825 ± 0.372
2.505ValGln: 2.505 ± 0.341
2.612ValArg: 2.612 ± 0.318
4.957ValSer: 4.957 ± 0.868
3.518ValThr: 3.518 ± 0.475
4.104ValVal: 4.104 ± 0.469
0.533ValTrp: 0.533 ± 0.179
2.238ValTyr: 2.238 ± 0.375
0.0ValXaa: 0.0 ± 0.0
Trp
0.586TrpAla: 0.586 ± 0.147
0.266TrpCys: 0.266 ± 0.119
0.48TrpAsp: 0.48 ± 0.209
0.586TrpGlu: 0.586 ± 0.14
0.48TrpPhe: 0.48 ± 0.151
0.586TrpGly: 0.586 ± 0.14
0.373TrpHis: 0.373 ± 0.158
0.959TrpIle: 0.959 ± 0.288
0.586TrpLys: 0.586 ± 0.186
1.013TrpLeu: 1.013 ± 0.286
0.426TrpMet: 0.426 ± 0.134
0.693TrpAsn: 0.693 ± 0.171
0.053TrpPro: 0.053 ± 0.064
0.426TrpGln: 0.426 ± 0.161
0.32TrpArg: 0.32 ± 0.141
0.693TrpSer: 0.693 ± 0.186
0.107TrpThr: 0.107 ± 0.075
0.799TrpVal: 0.799 ± 0.208
0.053TrpTrp: 0.053 ± 0.052
0.533TrpTyr: 0.533 ± 0.19
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.878TyrAla: 2.878 ± 0.342
0.586TyrCys: 0.586 ± 0.188
2.079TyrAsp: 2.079 ± 0.286
2.665TyrGlu: 2.665 ± 0.365
1.599TyrPhe: 1.599 ± 0.317
2.345TyrGly: 2.345 ± 0.513
0.693TyrHis: 0.693 ± 0.224
2.771TyrIle: 2.771 ± 0.531
2.612TyrLys: 2.612 ± 0.312
3.411TyrLeu: 3.411 ± 0.494
1.066TyrMet: 1.066 ± 0.231
2.558TyrAsn: 2.558 ± 0.341
1.386TyrPro: 1.386 ± 0.313
1.705TyrGln: 1.705 ± 0.308
1.652TyrArg: 1.652 ± 0.287
2.345TyrSer: 2.345 ± 0.386
1.972TyrThr: 1.972 ± 0.32
2.558TyrVal: 2.558 ± 0.347
0.533TyrTrp: 0.533 ± 0.186
1.546TyrTyr: 1.546 ± 0.286
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 77 proteins (18764 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski