Rhodococcus triatomae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4426 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G8F244|A0A1G8F244_9NOCA Uncharacterized protein OS=Rhodococcus triatomae OX=300028 GN=SAMN05444695_103146 PE=3 SV=1
MM1 pKa = 7.05NHH3 pKa = 6.39IPDD6 pKa = 4.63PEE8 pKa = 4.09EE9 pKa = 5.16SPDD12 pKa = 3.54GPVFEE17 pKa = 5.1GRR19 pKa = 11.84LLVRR23 pKa = 11.84PGDD26 pKa = 3.89EE27 pKa = 4.89IVDD30 pKa = 3.54QGAGFDD36 pKa = 3.23IATLVTRR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84VLSVLGVGAGALALAACSGGQARR68 pKa = 11.84TSSTTTTSASSTASEE83 pKa = 4.73EE84 pKa = 4.19IPEE87 pKa = 4.42EE88 pKa = 4.24TNGPYY93 pKa = 9.99PADD96 pKa = 3.46GTNGVNVLEE105 pKa = 4.3EE106 pKa = 4.33SGLVRR111 pKa = 11.84RR112 pKa = 11.84DD113 pKa = 3.41LTSSLDD119 pKa = 3.21GGTTVDD125 pKa = 3.65GTPLSFTFTVTDD137 pKa = 3.61MANDD141 pKa = 3.5NVPFEE146 pKa = 4.37GVAVYY151 pKa = 9.62AWQCDD156 pKa = 3.44AAGLYY161 pKa = 10.82SMYY164 pKa = 10.79SEE166 pKa = 4.18GVEE169 pKa = 4.0DD170 pKa = 3.49EE171 pKa = 4.51TYY173 pKa = 11.15LRR175 pKa = 11.84GIQIADD181 pKa = 3.53AEE183 pKa = 4.6GQVTLEE189 pKa = 4.35TIVPGCYY196 pKa = 8.81TGRR199 pKa = 11.84WTHH202 pKa = 5.39IHH204 pKa = 6.26FEE206 pKa = 4.26IYY208 pKa = 10.02PDD210 pKa = 3.95GDD212 pKa = 3.55SATDD216 pKa = 3.46VEE218 pKa = 4.44NAIATSQVAFPQDD231 pKa = 3.5MLDD234 pKa = 3.12EE235 pKa = 4.82VYY237 pKa = 10.68QLEE240 pKa = 4.81TYY242 pKa = 10.46AGSARR247 pKa = 11.84NLAAIGGLEE256 pKa = 3.87NDD258 pKa = 3.61NVFGDD263 pKa = 4.4GYY265 pKa = 8.92EE266 pKa = 4.08LQMGTFSGDD275 pKa = 3.24PDD277 pKa = 3.52SGYY280 pKa = 11.23VGSLPVAVDD289 pKa = 3.41TTTEE293 pKa = 3.99PAATGAPPGGPGGGAGPGGGRR314 pKa = 11.84PPGDD318 pKa = 3.52GGPPPGAPPNN328 pKa = 3.77

Molecular weight: 33.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G8J7T0|A0A1G8J7T0_9NOCA Clp amino terminal domain-containing protein pathogenicity island component OS=Rhodococcus triatomae OX=300028 GN=G4H72_14615 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.56LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 10.11LGKK33 pKa = 9.87

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4426

0

4426

1436612

30

8970

324.6

34.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.04 ± 0.044

0.681 ± 0.011

6.511 ± 0.031

5.733 ± 0.03

2.953 ± 0.017

9.24 ± 0.029

2.144 ± 0.018

3.846 ± 0.025

1.755 ± 0.025

9.935 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.79 ± 0.016

1.778 ± 0.018

5.77 ± 0.036

2.563 ± 0.02

7.697 ± 0.036

5.574 ± 0.026

6.215 ± 0.028

9.344 ± 0.037

1.431 ± 0.016

1.999 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski