Myripristis murdjan (pinecone soldierfish)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha;

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55955 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A667X8U4|A0A667X8U4_9TELE Mucolipin-2-like OS=Myripristis murdjan OX=586833 GN=LOC115358416 PE=4 SV=1
MM1 pKa = 7.74LPRR4 pKa = 11.84EE5 pKa = 4.29KK6 pKa = 10.62KK7 pKa = 10.16LLQPSEE13 pKa = 4.21PALSSVYY20 pKa = 10.3VEE22 pKa = 4.46EE23 pKa = 4.2PLYY26 pKa = 10.68PNDD29 pKa = 3.43GKK31 pKa = 9.98PWSKK35 pKa = 10.86CSLDD39 pKa = 3.77PSSKK43 pKa = 10.61SLQQSSGISSGGSSLGYY60 pKa = 10.61ANSGPDD66 pKa = 3.5IIASIQEE73 pKa = 4.43ALCKK77 pKa = 10.25ALPQLGQKK85 pKa = 10.32LPEE88 pKa = 4.86ADD90 pKa = 3.69AFLTEE95 pKa = 4.49SNKK98 pKa = 10.22NNSLLSTDD106 pKa = 4.04YY107 pKa = 10.89SPCSGRR113 pKa = 11.84DD114 pKa = 3.4DD115 pKa = 4.15DD116 pKa = 6.02LKK118 pKa = 11.44DD119 pKa = 3.66SGSSSFDD126 pKa = 2.93NRR128 pKa = 11.84TYY130 pKa = 10.96SILIPSIEE138 pKa = 4.14SQITSDD144 pKa = 3.09SSEE147 pKa = 3.99IQTQPAVICDD157 pKa = 3.57PAYY160 pKa = 10.29QPSEE164 pKa = 4.1GDD166 pKa = 3.6VMRR169 pKa = 11.84STSPDD174 pKa = 3.04QVVPVCLAAGQQDD187 pKa = 4.05AEE189 pKa = 4.57LPPAASSGLQTDD201 pKa = 5.28FSYY204 pKa = 11.04QPCNADD210 pKa = 3.41SGSSSSADD218 pKa = 3.31TSSLSSTSSDD228 pKa = 2.75INIAASCQIGAGEE241 pKa = 4.13EE242 pKa = 4.17DD243 pKa = 3.77GCEE246 pKa = 4.08DD247 pKa = 3.67SHH249 pKa = 6.73EE250 pKa = 4.43ADD252 pKa = 3.38SGAVNPAEE260 pKa = 4.2TSQDD264 pKa = 3.29VCGEE268 pKa = 3.95QAPVCDD274 pKa = 5.09ANPCYY279 pKa = 10.58GSLPAFSHH287 pKa = 6.65SSPIMDD293 pKa = 5.82DD294 pKa = 4.17DD295 pKa = 4.06YY296 pKa = 11.82QAFQSLVKK304 pKa = 10.73QPDD307 pKa = 3.11IALLEE312 pKa = 4.35EE313 pKa = 4.39SSGDD317 pKa = 3.34QWEE320 pKa = 4.24QPQLDD325 pKa = 3.84KK326 pKa = 11.31HH327 pKa = 6.18PEE329 pKa = 3.81EE330 pKa = 4.96SSTKK334 pKa = 9.7ISQSSSDD341 pKa = 3.26AVLPDD346 pKa = 4.37FSDD349 pKa = 3.25NAQAGRR355 pKa = 11.84CLPEE359 pKa = 4.15FEE361 pKa = 4.61TSLFSFLPSDD371 pKa = 3.21HH372 pKa = 6.95SEE374 pKa = 3.93AVITDD379 pKa = 3.66IEE381 pKa = 4.49SCAEE385 pKa = 3.94SGRR388 pKa = 11.84KK389 pKa = 8.66RR390 pKa = 11.84CHH392 pKa = 6.71ADD394 pKa = 3.11EE395 pKa = 5.63LDD397 pKa = 3.7LTRR400 pKa = 11.84QPLCFLFNN408 pKa = 3.96

Molecular weight: 43.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A668AH03|A0A668AH03_9TELE Sphingosine-1-phosphate phosphatase 1-like OS=Myripristis murdjan OX=586833 GN=LOC115355965 PE=4 SV=1
SS1 pKa = 6.49SNRR4 pKa = 11.84RR5 pKa = 11.84QTSNSRR11 pKa = 11.84QNSTSRR17 pKa = 11.84QIFSNRR23 pKa = 11.84QTSTSRR29 pKa = 11.84QISTSRR35 pKa = 11.84QNSTSRR41 pKa = 11.84QISSNRR47 pKa = 11.84QTSTSRR53 pKa = 11.84QISSKK58 pKa = 10.05RR59 pKa = 11.84QTSTSRR65 pKa = 11.84QTSSKK70 pKa = 9.79RR71 pKa = 11.84QTSTSRR77 pKa = 11.84QTSSLQTSTSCRR89 pKa = 11.84QNSTSRR95 pKa = 11.84QISSNRR101 pKa = 11.84QTSTSRR107 pKa = 11.84QTSSKK112 pKa = 9.79RR113 pKa = 11.84QTSTSRR119 pKa = 11.84QTSSKK124 pKa = 9.79RR125 pKa = 11.84QTSTSRR131 pKa = 11.84QTSILQTSTSWQTSTSRR148 pKa = 11.84QTSSKK153 pKa = 9.79RR154 pKa = 11.84QTSTSRR160 pKa = 11.84QNSTSRR166 pKa = 11.84QISSNRR172 pKa = 11.84QTSTSRR178 pKa = 11.84QTSSKK183 pKa = 9.79RR184 pKa = 11.84QTSTSRR190 pKa = 11.84QTSSLQTSTSWQKK203 pKa = 11.08LLQPANLHH211 pKa = 5.92QPTNLL216 pKa = 3.41

Molecular weight: 24.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23967

31988

55955

34307100

18

8699

613.1

68.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.686 ± 0.008

2.339 ± 0.008

5.187 ± 0.007

6.758 ± 0.012

3.825 ± 0.006

6.292 ± 0.011

2.69 ± 0.005

4.578 ± 0.007

5.596 ± 0.011

9.877 ± 0.012

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.005

3.802 ± 0.006

5.406 ± 0.012

4.678 ± 0.009

5.603 ± 0.007

8.135 ± 0.012

5.513 ± 0.007

6.512 ± 0.009

1.198 ± 0.003

2.902 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski