Streptomyces phage Galactica

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Hiyaavirus; unclassified Hiyaavirus

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 115 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M3T935|A0A6M3T935_9CAUD Uncharacterized protein OS=Streptomyces phage Galactica OX=2725755 GN=32 PE=4 SV=1
MM1 pKa = 7.66ANLVTNIGKK10 pKa = 9.71GRR12 pKa = 11.84FVYY15 pKa = 10.72YY16 pKa = 10.64AGLPAADD23 pKa = 4.59DD24 pKa = 3.82SLVAVVLEE32 pKa = 4.17ATGLEE37 pKa = 4.29SDD39 pKa = 5.71DD40 pKa = 4.95ALQDD44 pKa = 3.79YY45 pKa = 11.15DD46 pKa = 5.44DD47 pKa = 5.04LASLLAGASNEE58 pKa = 4.06QITMGRR64 pKa = 11.84KK65 pKa = 6.81TLVNVTVNVNDD76 pKa = 4.07AANSASIDD84 pKa = 3.74CDD86 pKa = 4.4DD87 pKa = 4.3ITWTAATGNATGKK100 pKa = 9.22MVICYY105 pKa = 9.82KK106 pKa = 9.27PTASATDD113 pKa = 3.33AQMIPLTLHH122 pKa = 6.75DD123 pKa = 5.02FSVTPDD129 pKa = 3.22GTDD132 pKa = 2.5ITVQINAAGLATAANAA148 pKa = 3.93

Molecular weight: 15.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M3T763|A0A6M3T763_9CAUD Membrane protein OS=Streptomyces phage Galactica OX=2725755 GN=116 PE=4 SV=1
MM1 pKa = 7.55PQFQTRR7 pKa = 11.84DD8 pKa = 3.36GEE10 pKa = 4.5LVSFEE15 pKa = 4.4SRR17 pKa = 11.84HH18 pKa = 6.52DD19 pKa = 3.56PVTPFAQVIWCWLVRR34 pKa = 11.84HH35 pKa = 6.31RR36 pKa = 11.84FPILLAVVNSIIIGSSTGGVVVTLVATYY64 pKa = 10.21LVVVLGYY71 pKa = 10.0RR72 pKa = 11.84VAKK75 pKa = 10.12RR76 pKa = 11.84AVSEE80 pKa = 3.83AAFWSRR86 pKa = 11.84KK87 pKa = 6.95MSAIDD92 pKa = 3.39SYY94 pKa = 10.0RR95 pKa = 11.84TRR97 pKa = 11.84AQRR100 pKa = 11.84MQQ102 pKa = 3.18

Molecular weight: 11.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

115

0

115

23756

32

1103

206.6

22.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.806 ± 0.397

0.993 ± 0.126

6.276 ± 0.231

6.146 ± 0.324

2.568 ± 0.121

8.604 ± 0.252

1.949 ± 0.121

3.911 ± 0.198

4.315 ± 0.241

7.447 ± 0.176

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.357 ± 0.11

3.338 ± 0.122

5.266 ± 0.274

3.797 ± 0.128

7.56 ± 0.231

5.375 ± 0.246

6.668 ± 0.319

7.981 ± 0.223

2.126 ± 0.123

2.517 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski