Amino acid dipepetide frequency for Streptomyces phage Galactica

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.008AlaAla: 13.008 ± 1.027
0.968AlaCys: 0.968 ± 0.217
7.114AlaAsp: 7.114 ± 0.482
8.04AlaGlu: 8.04 ± 0.752
2.399AlaPhe: 2.399 ± 0.3
7.325AlaGly: 7.325 ± 0.564
1.852AlaHis: 1.852 ± 0.463
3.957AlaIle: 3.957 ± 0.393
6.104AlaLys: 6.104 ± 0.766
8.293AlaLeu: 8.293 ± 0.744
2.694AlaMet: 2.694 ± 0.28
3.747AlaAsn: 3.747 ± 0.36
4.967AlaPro: 4.967 ± 0.799
5.094AlaGln: 5.094 ± 0.433
7.367AlaArg: 7.367 ± 0.627
5.893AlaSer: 5.893 ± 0.553
6.609AlaThr: 6.609 ± 0.689
7.493AlaVal: 7.493 ± 0.675
2.61AlaTrp: 2.61 ± 0.423
2.526AlaTyr: 2.526 ± 0.353
0.0AlaXaa: 0.0 ± 0.0
Cys
0.926CysAla: 0.926 ± 0.215
0.126CysCys: 0.126 ± 0.096
0.589CysAsp: 0.589 ± 0.13
0.337CysGlu: 0.337 ± 0.125
0.126CysPhe: 0.126 ± 0.093
1.221CysGly: 1.221 ± 0.235
0.253CysHis: 0.253 ± 0.118
0.21CysIle: 0.21 ± 0.116
0.589CysLys: 0.589 ± 0.185
0.589CysLeu: 0.589 ± 0.168
0.168CysMet: 0.168 ± 0.115
0.337CysAsn: 0.337 ± 0.112
0.674CysPro: 0.674 ± 0.19
0.379CysGln: 0.379 ± 0.112
0.758CysArg: 0.758 ± 0.179
0.8CysSer: 0.8 ± 0.175
0.589CysThr: 0.589 ± 0.189
0.631CysVal: 0.631 ± 0.182
0.337CysTrp: 0.337 ± 0.129
0.295CysTyr: 0.295 ± 0.105
0.0CysXaa: 0.0 ± 0.0
Asp
7.241AspAla: 7.241 ± 0.64
0.379AspCys: 0.379 ± 0.14
4.294AspAsp: 4.294 ± 0.424
4.252AspGlu: 4.252 ± 0.618
1.558AspPhe: 1.558 ± 0.239
5.262AspGly: 5.262 ± 0.401
1.431AspHis: 1.431 ± 0.26
2.61AspIle: 2.61 ± 0.363
1.936AspLys: 1.936 ± 0.292
5.851AspLeu: 5.851 ± 0.553
1.305AspMet: 1.305 ± 0.254
1.6AspAsn: 1.6 ± 0.228
3.999AspPro: 3.999 ± 0.367
2.231AspGln: 2.231 ± 0.326
5.136AspArg: 5.136 ± 0.485
2.989AspSer: 2.989 ± 0.382
3.957AspThr: 3.957 ± 0.324
4.041AspVal: 4.041 ± 0.438
0.968AspTrp: 0.968 ± 0.159
1.726AspTyr: 1.726 ± 0.305
0.0AspXaa: 0.0 ± 0.0
Glu
6.651GluAla: 6.651 ± 0.624
0.968GluCys: 0.968 ± 0.218
3.031GluAsp: 3.031 ± 0.345
3.957GluGlu: 3.957 ± 0.573
2.273GluPhe: 2.273 ± 0.281
4.589GluGly: 4.589 ± 0.418
1.179GluHis: 1.179 ± 0.24
2.863GluIle: 2.863 ± 0.438
1.768GluLys: 1.768 ± 0.3
5.052GluLeu: 5.052 ± 0.449
1.684GluMet: 1.684 ± 0.252
2.021GluAsn: 2.021 ± 0.289
2.863GluPro: 2.863 ± 0.445
2.863GluGln: 2.863 ± 0.388
4.546GluArg: 4.546 ± 0.513
2.442GluSer: 2.442 ± 0.347
3.831GluThr: 3.831 ± 0.48
5.893GluVal: 5.893 ± 0.537
1.515GluTrp: 1.515 ± 0.232
1.473GluTyr: 1.473 ± 0.291
0.0GluXaa: 0.0 ± 0.0
Phe
2.273PheAla: 2.273 ± 0.293
0.084PheCys: 0.084 ± 0.058
1.81PheAsp: 1.81 ± 0.291
1.852PheGlu: 1.852 ± 0.287
0.884PhePhe: 0.884 ± 0.213
2.568PheGly: 2.568 ± 0.359
0.547PheHis: 0.547 ± 0.153
1.052PheIle: 1.052 ± 0.198
1.137PheLys: 1.137 ± 0.203
1.684PheLeu: 1.684 ± 0.285
0.505PheMet: 0.505 ± 0.141
0.968PheAsn: 0.968 ± 0.229
1.095PhePro: 1.095 ± 0.215
0.884PheGln: 0.884 ± 0.204
2.231PheArg: 2.231 ± 0.276
1.515PheSer: 1.515 ± 0.263
1.221PheThr: 1.221 ± 0.225
1.936PheVal: 1.936 ± 0.346
0.463PheTrp: 0.463 ± 0.184
0.968PheTyr: 0.968 ± 0.187
0.0PheXaa: 0.0 ± 0.0
Gly
7.367GlyAla: 7.367 ± 0.726
1.221GlyCys: 1.221 ± 0.238
4.967GlyAsp: 4.967 ± 0.499
4.252GlyGlu: 4.252 ± 0.433
2.568GlyPhe: 2.568 ± 0.3
7.998GlyGly: 7.998 ± 0.77
2.694GlyHis: 2.694 ± 0.364
3.073GlyIle: 3.073 ± 0.372
3.662GlyLys: 3.662 ± 0.356
6.146GlyLeu: 6.146 ± 0.544
1.894GlyMet: 1.894 ± 0.28
2.61GlyAsn: 2.61 ± 0.306
3.284GlyPro: 3.284 ± 0.375
3.62GlyGln: 3.62 ± 0.334
7.367GlyArg: 7.367 ± 0.699
5.178GlySer: 5.178 ± 0.609
6.062GlyThr: 6.062 ± 0.68
7.704GlyVal: 7.704 ± 0.584
1.979GlyTrp: 1.979 ± 0.264
2.399GlyTyr: 2.399 ± 0.34
0.0GlyXaa: 0.0 ± 0.0
His
2.105HisAla: 2.105 ± 0.286
0.253HisCys: 0.253 ± 0.104
1.558HisAsp: 1.558 ± 0.253
1.473HisGlu: 1.473 ± 0.23
0.379HisPhe: 0.379 ± 0.115
1.431HisGly: 1.431 ± 0.244
0.421HisHis: 0.421 ± 0.151
0.758HisIle: 0.758 ± 0.188
0.968HisLys: 0.968 ± 0.189
1.515HisLeu: 1.515 ± 0.268
0.589HisMet: 0.589 ± 0.147
0.505HisAsn: 0.505 ± 0.144
1.515HisPro: 1.515 ± 0.233
0.589HisGln: 0.589 ± 0.14
1.431HisArg: 1.431 ± 0.268
0.842HisSer: 0.842 ± 0.186
1.179HisThr: 1.179 ± 0.228
1.263HisVal: 1.263 ± 0.246
0.253HisTrp: 0.253 ± 0.098
0.463HisTyr: 0.463 ± 0.13
0.0HisXaa: 0.0 ± 0.0
Ile
2.61IleAla: 2.61 ± 0.415
0.463IleCys: 0.463 ± 0.156
3.662IleAsp: 3.662 ± 0.337
3.199IleGlu: 3.199 ± 0.416
0.716IlePhe: 0.716 ± 0.166
3.494IleGly: 3.494 ± 0.347
0.337IleHis: 0.337 ± 0.111
1.431IleIle: 1.431 ± 0.316
2.231IleLys: 2.231 ± 0.499
1.979IleLeu: 1.979 ± 0.273
0.8IleMet: 0.8 ± 0.174
1.768IleAsn: 1.768 ± 0.269
1.852IlePro: 1.852 ± 0.366
1.726IleGln: 1.726 ± 0.237
3.199IleArg: 3.199 ± 0.391
2.063IleSer: 2.063 ± 0.305
3.41IleThr: 3.41 ± 0.481
2.484IleVal: 2.484 ± 0.351
0.674IleTrp: 0.674 ± 0.161
1.01IleTyr: 1.01 ± 0.218
0.0IleXaa: 0.0 ± 0.0
Lys
5.767LysAla: 5.767 ± 0.527
0.295LysCys: 0.295 ± 0.126
2.315LysAsp: 2.315 ± 0.334
2.273LysGlu: 2.273 ± 0.357
1.263LysPhe: 1.263 ± 0.208
3.241LysGly: 3.241 ± 0.421
1.01LysHis: 1.01 ± 0.206
1.684LysIle: 1.684 ± 0.317
1.81LysLys: 1.81 ± 0.367
3.747LysLeu: 3.747 ± 0.451
0.926LysMet: 0.926 ± 0.217
0.926LysAsn: 0.926 ± 0.22
2.273LysPro: 2.273 ± 0.393
1.894LysGln: 1.894 ± 0.307
3.199LysArg: 3.199 ± 0.459
2.105LysSer: 2.105 ± 0.312
2.694LysThr: 2.694 ± 0.388
3.662LysVal: 3.662 ± 0.392
1.179LysTrp: 1.179 ± 0.229
0.842LysTyr: 0.842 ± 0.182
0.0LysXaa: 0.0 ± 0.0
Leu
8.419LeuAla: 8.419 ± 0.619
0.547LeuCys: 0.547 ± 0.139
5.557LeuAsp: 5.557 ± 0.464
4.041LeuGlu: 4.041 ± 0.523
1.642LeuPhe: 1.642 ± 0.27
6.946LeuGly: 6.946 ± 0.473
1.137LeuHis: 1.137 ± 0.218
3.326LeuIle: 3.326 ± 0.337
3.536LeuLys: 3.536 ± 0.41
6.23LeuLeu: 6.23 ± 0.616
2.189LeuMet: 2.189 ± 0.297
2.484LeuAsn: 2.484 ± 0.372
4.378LeuPro: 4.378 ± 0.422
1.852LeuGln: 1.852 ± 0.287
5.936LeuArg: 5.936 ± 0.579
2.736LeuSer: 2.736 ± 0.355
4.883LeuThr: 4.883 ± 0.467
6.23LeuVal: 6.23 ± 0.542
1.052LeuTrp: 1.052 ± 0.241
1.347LeuTyr: 1.347 ± 0.199
0.0LeuXaa: 0.0 ± 0.0
Met
2.863MetAla: 2.863 ± 0.359
0.21MetCys: 0.21 ± 0.1
1.642MetAsp: 1.642 ± 0.291
1.179MetGlu: 1.179 ± 0.247
0.505MetPhe: 0.505 ± 0.138
1.6MetGly: 1.6 ± 0.328
0.379MetHis: 0.379 ± 0.14
1.01MetIle: 1.01 ± 0.191
0.758MetLys: 0.758 ± 0.173
1.305MetLeu: 1.305 ± 0.175
0.674MetMet: 0.674 ± 0.205
0.674MetAsn: 0.674 ± 0.183
1.642MetPro: 1.642 ± 0.24
0.842MetGln: 0.842 ± 0.201
1.473MetArg: 1.473 ± 0.244
1.936MetSer: 1.936 ± 0.272
1.684MetThr: 1.684 ± 0.262
2.063MetVal: 2.063 ± 0.324
0.631MetTrp: 0.631 ± 0.186
0.505MetTyr: 0.505 ± 0.152
0.0MetXaa: 0.0 ± 0.0
Asn
4.125AsnAla: 4.125 ± 0.642
0.421AsnCys: 0.421 ± 0.137
1.768AsnAsp: 1.768 ± 0.283
1.6AsnGlu: 1.6 ± 0.219
0.758AsnPhe: 0.758 ± 0.161
3.873AsnGly: 3.873 ± 0.358
0.379AsnHis: 0.379 ± 0.139
1.221AsnIle: 1.221 ± 0.224
0.926AsnLys: 0.926 ± 0.192
2.442AsnLeu: 2.442 ± 0.389
0.505AsnMet: 0.505 ± 0.163
0.926AsnAsn: 0.926 ± 0.176
2.147AsnPro: 2.147 ± 0.291
1.305AsnGln: 1.305 ± 0.183
2.231AsnArg: 2.231 ± 0.286
2.063AsnSer: 2.063 ± 0.306
2.526AsnThr: 2.526 ± 0.379
2.778AsnVal: 2.778 ± 0.337
0.716AsnTrp: 0.716 ± 0.139
0.674AsnTyr: 0.674 ± 0.176
0.0AsnXaa: 0.0 ± 0.0
Pro
5.936ProAla: 5.936 ± 0.739
0.421ProCys: 0.421 ± 0.165
3.368ProAsp: 3.368 ± 0.362
4.42ProGlu: 4.42 ± 0.529
0.842ProPhe: 0.842 ± 0.223
5.43ProGly: 5.43 ± 0.49
0.968ProHis: 0.968 ± 0.177
1.642ProIle: 1.642 ± 0.25
2.484ProLys: 2.484 ± 0.379
2.989ProLeu: 2.989 ± 0.396
1.305ProMet: 1.305 ± 0.28
2.147ProAsn: 2.147 ± 0.31
3.073ProPro: 3.073 ± 0.455
1.852ProGln: 1.852 ± 0.322
3.241ProArg: 3.241 ± 0.357
3.326ProSer: 3.326 ± 0.394
3.494ProThr: 3.494 ± 0.456
3.747ProVal: 3.747 ± 0.32
1.01ProTrp: 1.01 ± 0.211
0.968ProTyr: 0.968 ± 0.174
0.0ProXaa: 0.0 ± 0.0
Gln
4.42GlnAla: 4.42 ± 0.375
0.421GlnCys: 0.421 ± 0.136
1.979GlnAsp: 1.979 ± 0.348
1.726GlnGlu: 1.726 ± 0.295
1.389GlnPhe: 1.389 ± 0.235
3.241GlnGly: 3.241 ± 0.385
0.884GlnHis: 0.884 ± 0.174
1.642GlnIle: 1.642 ± 0.29
1.137GlnLys: 1.137 ± 0.232
3.326GlnLeu: 3.326 ± 0.392
1.095GlnMet: 1.095 ± 0.202
1.221GlnAsn: 1.221 ± 0.249
2.021GlnPro: 2.021 ± 0.299
1.221GlnGln: 1.221 ± 0.235
2.652GlnArg: 2.652 ± 0.332
1.852GlnSer: 1.852 ± 0.399
2.526GlnThr: 2.526 ± 0.362
3.578GlnVal: 3.578 ± 0.398
0.631GlnTrp: 0.631 ± 0.163
1.01GlnTyr: 1.01 ± 0.214
0.0GlnXaa: 0.0 ± 0.0
Arg
7.283ArgAla: 7.283 ± 0.597
0.716ArgCys: 0.716 ± 0.197
4.294ArgAsp: 4.294 ± 0.404
4.589ArgGlu: 4.589 ± 0.479
1.726ArgPhe: 1.726 ± 0.243
4.841ArgGly: 4.841 ± 0.526
2.063ArgHis: 2.063 ± 0.317
2.652ArgIle: 2.652 ± 0.369
4.168ArgLys: 4.168 ± 0.468
6.062ArgLeu: 6.062 ± 0.545
2.189ArgMet: 2.189 ± 0.285
2.652ArgAsn: 2.652 ± 0.41
4.21ArgPro: 4.21 ± 0.41
3.199ArgGln: 3.199 ± 0.495
5.767ArgArg: 5.767 ± 0.597
3.662ArgSer: 3.662 ± 0.463
4.673ArgThr: 4.673 ± 0.522
6.862ArgVal: 6.862 ± 0.643
1.642ArgTrp: 1.642 ± 0.265
2.357ArgTyr: 2.357 ± 0.397
0.0ArgXaa: 0.0 ± 0.0
Ser
6.23SerAla: 6.23 ± 0.655
0.379SerCys: 0.379 ± 0.127
2.315SerAsp: 2.315 ± 0.319
2.568SerGlu: 2.568 ± 0.331
1.052SerPhe: 1.052 ± 0.21
6.735SerGly: 6.735 ± 0.903
1.01SerHis: 1.01 ± 0.228
1.894SerIle: 1.894 ± 0.323
2.526SerLys: 2.526 ± 0.404
3.494SerLeu: 3.494 ± 0.426
1.389SerMet: 1.389 ± 0.236
1.979SerAsn: 1.979 ± 0.306
2.905SerPro: 2.905 ± 0.34
1.431SerGln: 1.431 ± 0.262
3.999SerArg: 3.999 ± 0.454
3.284SerSer: 3.284 ± 0.47
4.041SerThr: 4.041 ± 0.645
3.831SerVal: 3.831 ± 0.369
1.095SerTrp: 1.095 ± 0.212
1.6SerTyr: 1.6 ± 0.279
0.0SerXaa: 0.0 ± 0.0
Thr
8.04ThrAla: 8.04 ± 1.11
0.842ThrCys: 0.842 ± 0.184
4.125ThrAsp: 4.125 ± 0.52
3.873ThrGlu: 3.873 ± 0.385
2.273ThrPhe: 2.273 ± 0.346
6.02ThrGly: 6.02 ± 0.513
0.674ThrHis: 0.674 ± 0.191
2.526ThrIle: 2.526 ± 0.363
2.61ThrLys: 2.61 ± 0.432
5.094ThrLeu: 5.094 ± 0.416
1.052ThrMet: 1.052 ± 0.191
2.231ThrAsn: 2.231 ± 0.277
4.42ThrPro: 4.42 ± 0.599
1.936ThrGln: 1.936 ± 0.316
4.462ThrArg: 4.462 ± 0.448
4.252ThrSer: 4.252 ± 0.601
4.589ThrThr: 4.589 ± 0.628
4.631ThrVal: 4.631 ± 0.447
1.389ThrTrp: 1.389 ± 0.261
1.642ThrTyr: 1.642 ± 0.286
0.0ThrXaa: 0.0 ± 0.0
Val
8.588ValAla: 8.588 ± 0.985
0.631ValCys: 0.631 ± 0.198
4.504ValAsp: 4.504 ± 0.549
5.094ValGlu: 5.094 ± 0.562
2.442ValPhe: 2.442 ± 0.323
6.483ValGly: 6.483 ± 0.555
1.473ValHis: 1.473 ± 0.222
3.704ValIle: 3.704 ± 0.44
3.115ValLys: 3.115 ± 0.447
5.304ValLeu: 5.304 ± 0.515
1.558ValMet: 1.558 ± 0.257
2.82ValAsn: 2.82 ± 0.319
3.452ValPro: 3.452 ± 0.363
3.326ValGln: 3.326 ± 0.391
6.399ValArg: 6.399 ± 0.623
4.378ValSer: 4.378 ± 0.41
5.304ValThr: 5.304 ± 0.516
7.325ValVal: 7.325 ± 0.691
1.642ValTrp: 1.642 ± 0.259
2.273ValTyr: 2.273 ± 0.277
0.0ValXaa: 0.0 ± 0.0
Trp
2.063TrpAla: 2.063 ± 0.272
0.253TrpCys: 0.253 ± 0.111
1.768TrpAsp: 1.768 ± 0.228
1.01TrpGlu: 1.01 ± 0.214
0.463TrpPhe: 0.463 ± 0.142
1.389TrpGly: 1.389 ± 0.214
0.337TrpHis: 0.337 ± 0.122
0.631TrpIle: 0.631 ± 0.205
0.505TrpLys: 0.505 ± 0.165
1.81TrpLeu: 1.81 ± 0.345
0.463TrpMet: 0.463 ± 0.146
0.884TrpAsn: 0.884 ± 0.229
0.842TrpPro: 0.842 ± 0.191
0.631TrpGln: 0.631 ± 0.145
2.063TrpArg: 2.063 ± 0.335
1.221TrpSer: 1.221 ± 0.235
1.81TrpThr: 1.81 ± 0.279
1.979TrpVal: 1.979 ± 0.304
0.589TrpTrp: 0.589 ± 0.165
0.547TrpTyr: 0.547 ± 0.127
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.147TyrAla: 2.147 ± 0.297
0.337TyrCys: 0.337 ± 0.126
2.105TyrAsp: 2.105 ± 0.225
1.726TyrGlu: 1.726 ± 0.268
0.421TyrPhe: 0.421 ± 0.13
2.357TyrGly: 2.357 ± 0.281
0.463TyrHis: 0.463 ± 0.126
1.221TyrIle: 1.221 ± 0.192
1.179TyrLys: 1.179 ± 0.218
1.558TyrLeu: 1.558 ± 0.24
0.547TyrMet: 0.547 ± 0.145
0.884TyrAsn: 0.884 ± 0.186
1.052TyrPro: 1.052 ± 0.244
1.095TyrGln: 1.095 ± 0.249
2.147TyrArg: 2.147 ± 0.291
1.221TyrSer: 1.221 ± 0.236
1.6TyrThr: 1.6 ± 0.266
1.684TyrVal: 1.684 ± 0.29
0.884TyrTrp: 0.884 ± 0.185
0.547TyrTyr: 0.547 ± 0.184
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 115 proteins (23756 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski