Turnip vein-clearing virus (TVCV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Virgaviridae; Tobamovirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q88922|CAPSD_TVCV Capsid protein OS=Turnip vein-clearing virus OX=29272 GN=CP PE=3 SV=3
MM1 pKa = 7.8SYY3 pKa = 11.16NITNPNQYY11 pKa = 10.54QYY13 pKa = 11.16FAAVWAEE20 pKa = 4.56PIPMLNQCMSALSQSYY36 pKa = 7.17QTQAARR42 pKa = 11.84DD43 pKa = 3.67TVRR46 pKa = 11.84QQFSNLLSAVVTPSQRR62 pKa = 11.84FPDD65 pKa = 3.64TGSRR69 pKa = 11.84VYY71 pKa = 11.19VNSAVIKK78 pKa = 9.24PLYY81 pKa = 8.18EE82 pKa = 5.16ALMKK86 pKa = 11.02SFDD89 pKa = 3.38TRR91 pKa = 11.84NRR93 pKa = 11.84IIEE96 pKa = 4.26TEE98 pKa = 4.13EE99 pKa = 3.68EE100 pKa = 4.31SRR102 pKa = 11.84PSASEE107 pKa = 3.81VANATQRR114 pKa = 11.84VDD116 pKa = 3.42DD117 pKa = 3.9ATVAIRR123 pKa = 11.84SQIQLLLSEE132 pKa = 4.98LSNGHH137 pKa = 6.27GYY139 pKa = 8.47MNRR142 pKa = 11.84AEE144 pKa = 5.04FEE146 pKa = 4.26ALLPWTTAPATT157 pKa = 3.64

Molecular weight: 17.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q88922|CAPSD_TVCV Capsid protein OS=Turnip vein-clearing virus OX=29272 GN=CP PE=3 SV=3
MM1 pKa = 7.52SIVSYY6 pKa = 9.68EE7 pKa = 4.04PKK9 pKa = 10.66VSDD12 pKa = 4.59FLNLSKK18 pKa = 10.84KK19 pKa = 10.27EE20 pKa = 4.02EE21 pKa = 4.19ILPKK25 pKa = 10.55ALTRR29 pKa = 11.84LKK31 pKa = 9.72TVSISTKK38 pKa = 10.34DD39 pKa = 3.21IISVKK44 pKa = 10.06EE45 pKa = 4.02SEE47 pKa = 4.51TLCDD51 pKa = 3.78IDD53 pKa = 5.79LLINVPLDD61 pKa = 2.98KK62 pKa = 10.73YY63 pKa = 10.55RR64 pKa = 11.84YY65 pKa = 8.77VGILGAVFTGEE76 pKa = 3.81WLVPDD81 pKa = 4.61FVKK84 pKa = 10.95GGVTISVIDD93 pKa = 3.6KK94 pKa = 10.31RR95 pKa = 11.84LVNSKK100 pKa = 9.56EE101 pKa = 4.27CVIGTYY107 pKa = 10.02RR108 pKa = 11.84AAAKK112 pKa = 10.17SKK114 pKa = 9.9RR115 pKa = 11.84FQFKK119 pKa = 10.1LVPNYY124 pKa = 9.64FVSTVDD130 pKa = 3.6AKK132 pKa = 10.41RR133 pKa = 11.84KK134 pKa = 7.14PWQVHH139 pKa = 5.15VRR141 pKa = 11.84IQDD144 pKa = 3.65LKK146 pKa = 10.98IEE148 pKa = 4.82AGWQPLALEE157 pKa = 4.52VVSVAMVTNNVVMKK171 pKa = 9.81GLRR174 pKa = 11.84EE175 pKa = 3.91KK176 pKa = 10.85VVAINDD182 pKa = 3.79PDD184 pKa = 3.79VEE186 pKa = 4.25GFEE189 pKa = 4.43GVVDD193 pKa = 3.83EE194 pKa = 5.46FVDD197 pKa = 4.16SVAAFKK203 pKa = 10.95AVDD206 pKa = 3.38NFRR209 pKa = 11.84KK210 pKa = 9.79RR211 pKa = 11.84KK212 pKa = 9.58KK213 pKa = 9.95KK214 pKa = 9.75VEE216 pKa = 3.92EE217 pKa = 3.86RR218 pKa = 11.84DD219 pKa = 3.54VVSKK223 pKa = 10.67YY224 pKa = 10.41KK225 pKa = 10.53YY226 pKa = 10.04RR227 pKa = 11.84PEE229 pKa = 4.79KK230 pKa = 10.4YY231 pKa = 10.16AGPDD235 pKa = 3.12SFNLKK240 pKa = 10.02EE241 pKa = 4.15EE242 pKa = 4.36NVLQHH247 pKa = 6.13YY248 pKa = 9.65KK249 pKa = 10.35PEE251 pKa = 4.3SVPVLRR257 pKa = 11.84SGVGRR262 pKa = 11.84AHH264 pKa = 6.43TNAA267 pKa = 4.02

Molecular weight: 30.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2025

157

1601

675.0

76.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.358 ± 0.998

1.975 ± 0.639

6.173 ± 0.807

6.37 ± 0.255

5.136 ± 0.717

4.346 ± 0.593

2.123 ± 0.606

5.185 ± 0.183

7.358 ± 1.593

8.938 ± 0.696

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.412

4.395 ± 0.489

3.951 ± 0.563

3.407 ± 1.033

4.741 ± 0.515

6.765 ± 0.893

5.679 ± 0.668

8.741 ± 1.862

1.136 ± 0.035

3.802 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski