Amino acid dipepetide frequency for Mycobacterium phage Demsculpinboyz

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.003AlaAla: 13.003 ± 1.191
1.021AlaCys: 1.021 ± 0.26
6.287AlaAsp: 6.287 ± 0.533
8.651AlaGlu: 8.651 ± 0.996
2.794AlaPhe: 2.794 ± 0.382
8.705AlaGly: 8.705 ± 1.0
2.203AlaHis: 2.203 ± 0.335
4.299AlaIle: 4.299 ± 0.491
4.836AlaLys: 4.836 ± 0.597
9.833AlaLeu: 9.833 ± 0.821
3.17AlaMet: 3.17 ± 0.426
3.278AlaAsn: 3.278 ± 0.492
4.03AlaPro: 4.03 ± 0.416
4.084AlaGln: 4.084 ± 0.545
5.857AlaArg: 5.857 ± 0.684
4.513AlaSer: 4.513 ± 0.55
4.352AlaThr: 4.352 ± 0.522
7.791AlaVal: 7.791 ± 0.611
2.633AlaTrp: 2.633 ± 0.378
2.042AlaTyr: 2.042 ± 0.337
0.0AlaXaa: 0.0 ± 0.0
Cys
1.128CysAla: 1.128 ± 0.255
0.269CysCys: 0.269 ± 0.112
0.86CysAsp: 0.86 ± 0.214
1.236CysGlu: 1.236 ± 0.258
0.322CysPhe: 0.322 ± 0.128
1.881CysGly: 1.881 ± 0.383
0.484CysHis: 0.484 ± 0.151
0.322CysIle: 0.322 ± 0.131
0.322CysLys: 0.322 ± 0.122
0.645CysLeu: 0.645 ± 0.211
0.107CysMet: 0.107 ± 0.07
0.537CysAsn: 0.537 ± 0.192
0.967CysPro: 0.967 ± 0.25
0.537CysGln: 0.537 ± 0.19
0.752CysArg: 0.752 ± 0.207
1.182CysSer: 1.182 ± 0.296
0.591CysThr: 0.591 ± 0.174
0.967CysVal: 0.967 ± 0.276
0.269CysTrp: 0.269 ± 0.122
0.322CysTyr: 0.322 ± 0.114
0.0CysXaa: 0.0 ± 0.0
Asp
6.716AspAla: 6.716 ± 0.636
0.967AspCys: 0.967 ± 0.224
4.621AspAsp: 4.621 ± 0.674
5.319AspGlu: 5.319 ± 0.63
2.042AspPhe: 2.042 ± 0.317
5.319AspGly: 5.319 ± 0.526
1.236AspHis: 1.236 ± 0.313
3.493AspIle: 3.493 ± 0.545
2.31AspLys: 2.31 ± 0.404
4.406AspLeu: 4.406 ± 0.546
1.451AspMet: 1.451 ± 0.305
2.042AspAsn: 2.042 ± 0.369
3.761AspPro: 3.761 ± 0.439
1.881AspGln: 1.881 ± 0.347
4.513AspArg: 4.513 ± 0.46
2.525AspSer: 2.525 ± 0.407
3.922AspThr: 3.922 ± 0.409
4.299AspVal: 4.299 ± 0.417
1.397AspTrp: 1.397 ± 0.286
1.504AspTyr: 1.504 ± 0.271
0.0AspXaa: 0.0 ± 0.0
Glu
6.179GluAla: 6.179 ± 0.723
1.128GluCys: 1.128 ± 0.246
4.191GluAsp: 4.191 ± 0.605
3.654GluGlu: 3.654 ± 0.58
1.988GluPhe: 1.988 ± 0.359
4.352GluGly: 4.352 ± 0.483
1.504GluHis: 1.504 ± 0.306
2.149GluIle: 2.149 ± 0.403
2.096GluLys: 2.096 ± 0.326
6.448GluLeu: 6.448 ± 0.623
1.075GluMet: 1.075 ± 0.21
2.042GluAsn: 2.042 ± 0.293
3.761GluPro: 3.761 ± 0.55
3.116GluGln: 3.116 ± 0.359
4.621GluArg: 4.621 ± 0.64
3.331GluSer: 3.331 ± 0.515
3.224GluThr: 3.224 ± 0.452
5.373GluVal: 5.373 ± 0.585
1.504GluTrp: 1.504 ± 0.252
1.773GluTyr: 1.773 ± 0.334
0.0GluXaa: 0.0 ± 0.0
Phe
2.794PheAla: 2.794 ± 0.331
0.376PheCys: 0.376 ± 0.151
2.525PheAsp: 2.525 ± 0.395
1.719PheGlu: 1.719 ± 0.303
0.645PhePhe: 0.645 ± 0.196
2.364PheGly: 2.364 ± 0.39
0.591PheHis: 0.591 ± 0.173
1.29PheIle: 1.29 ± 0.277
1.128PheLys: 1.128 ± 0.294
2.149PheLeu: 2.149 ± 0.386
0.86PheMet: 0.86 ± 0.202
0.913PheAsn: 0.913 ± 0.173
1.451PhePro: 1.451 ± 0.229
0.806PheGln: 0.806 ± 0.208
1.719PheArg: 1.719 ± 0.32
2.042PheSer: 2.042 ± 0.305
2.364PheThr: 2.364 ± 0.372
1.827PheVal: 1.827 ± 0.34
0.591PheTrp: 0.591 ± 0.155
0.484PheTyr: 0.484 ± 0.149
0.0PheXaa: 0.0 ± 0.0
Gly
8.006GlyAla: 8.006 ± 0.969
1.128GlyCys: 1.128 ± 0.259
4.46GlyAsp: 4.46 ± 0.495
4.89GlyGlu: 4.89 ± 0.486
2.525GlyPhe: 2.525 ± 0.317
10.155GlyGly: 10.155 ± 1.866
1.827GlyHis: 1.827 ± 0.303
4.245GlyIle: 4.245 ± 0.485
3.439GlyLys: 3.439 ± 0.461
5.696GlyLeu: 5.696 ± 0.693
1.827GlyMet: 1.827 ± 0.31
2.633GlyAsn: 2.633 ± 0.414
3.761GlyPro: 3.761 ± 0.449
3.654GlyGln: 3.654 ± 0.48
5.373GlyArg: 5.373 ± 0.471
5.373GlySer: 5.373 ± 0.992
6.716GlyThr: 6.716 ± 0.706
6.502GlyVal: 6.502 ± 0.646
2.794GlyTrp: 2.794 ± 0.396
2.364GlyTyr: 2.364 ± 0.368
0.0GlyXaa: 0.0 ± 0.0
His
1.558HisAla: 1.558 ± 0.29
0.537HisCys: 0.537 ± 0.18
1.397HisAsp: 1.397 ± 0.273
1.075HisGlu: 1.075 ± 0.239
0.591HisPhe: 0.591 ± 0.162
2.042HisGly: 2.042 ± 0.283
1.021HisHis: 1.021 ± 0.209
1.128HisIle: 1.128 ± 0.225
0.967HisLys: 0.967 ± 0.224
1.719HisLeu: 1.719 ± 0.36
0.484HisMet: 0.484 ± 0.151
0.913HisAsn: 0.913 ± 0.251
1.021HisPro: 1.021 ± 0.234
1.075HisGln: 1.075 ± 0.261
1.719HisArg: 1.719 ± 0.345
1.236HisSer: 1.236 ± 0.214
1.451HisThr: 1.451 ± 0.288
1.397HisVal: 1.397 ± 0.315
0.484HisTrp: 0.484 ± 0.136
0.806HisTyr: 0.806 ± 0.224
0.0HisXaa: 0.0 ± 0.0
Ile
4.728IleAla: 4.728 ± 0.599
0.752IleCys: 0.752 ± 0.246
3.869IleAsp: 3.869 ± 0.523
3.009IleGlu: 3.009 ± 0.369
1.021IlePhe: 1.021 ± 0.189
4.621IleGly: 4.621 ± 0.565
1.504IleHis: 1.504 ± 0.284
2.096IleIle: 2.096 ± 0.373
1.451IleLys: 1.451 ± 0.266
2.418IleLeu: 2.418 ± 0.412
0.752IleMet: 0.752 ± 0.208
1.451IleAsn: 1.451 ± 0.287
2.525IlePro: 2.525 ± 0.366
1.504IleGln: 1.504 ± 0.338
3.278IleArg: 3.278 ± 0.443
2.525IleSer: 2.525 ± 0.46
3.6IleThr: 3.6 ± 0.619
2.794IleVal: 2.794 ± 0.309
0.699IleTrp: 0.699 ± 0.2
1.021IleTyr: 1.021 ± 0.229
0.0IleXaa: 0.0 ± 0.0
Lys
4.191LysAla: 4.191 ± 0.523
0.43LysCys: 0.43 ± 0.153
1.934LysAsp: 1.934 ± 0.323
1.773LysGlu: 1.773 ± 0.322
0.806LysPhe: 0.806 ± 0.181
2.525LysGly: 2.525 ± 0.36
1.075LysHis: 1.075 ± 0.268
1.504LysIle: 1.504 ± 0.268
1.504LysLys: 1.504 ± 0.32
2.579LysLeu: 2.579 ± 0.395
0.806LysMet: 0.806 ± 0.197
1.128LysAsn: 1.128 ± 0.299
3.493LysPro: 3.493 ± 0.481
1.666LysGln: 1.666 ± 0.376
3.116LysArg: 3.116 ± 0.482
2.149LysSer: 2.149 ± 0.364
1.988LysThr: 1.988 ± 0.377
2.418LysVal: 2.418 ± 0.415
0.913LysTrp: 0.913 ± 0.206
1.075LysTyr: 1.075 ± 0.243
0.0LysXaa: 0.0 ± 0.0
Leu
9.887LeuAla: 9.887 ± 0.94
0.752LeuCys: 0.752 ± 0.23
4.997LeuAsp: 4.997 ± 0.555
3.707LeuGlu: 3.707 ± 0.468
2.31LeuPhe: 2.31 ± 0.351
6.716LeuGly: 6.716 ± 0.728
1.182LeuHis: 1.182 ± 0.278
3.6LeuIle: 3.6 ± 0.484
3.654LeuLys: 3.654 ± 0.471
5.91LeuLeu: 5.91 ± 0.696
1.343LeuMet: 1.343 ± 0.308
2.472LeuAsn: 2.472 ± 0.377
4.997LeuPro: 4.997 ± 0.504
2.848LeuGln: 2.848 ± 0.392
5.266LeuArg: 5.266 ± 0.63
5.481LeuSer: 5.481 ± 0.614
5.964LeuThr: 5.964 ± 0.558
4.728LeuVal: 4.728 ± 0.545
0.699LeuTrp: 0.699 ± 0.228
1.934LeuTyr: 1.934 ± 0.317
0.0LeuXaa: 0.0 ± 0.0
Met
2.472MetAla: 2.472 ± 0.38
0.215MetCys: 0.215 ± 0.12
0.967MetAsp: 0.967 ± 0.231
0.752MetGlu: 0.752 ± 0.194
0.86MetPhe: 0.86 ± 0.247
1.343MetGly: 1.343 ± 0.304
0.322MetHis: 0.322 ± 0.132
1.236MetIle: 1.236 ± 0.225
0.752MetLys: 0.752 ± 0.234
1.451MetLeu: 1.451 ± 0.25
0.322MetMet: 0.322 ± 0.139
0.699MetAsn: 0.699 ± 0.172
1.397MetPro: 1.397 ± 0.248
0.484MetGln: 0.484 ± 0.167
1.29MetArg: 1.29 ± 0.219
2.848MetSer: 2.848 ± 0.436
2.31MetThr: 2.31 ± 0.331
1.182MetVal: 1.182 ± 0.208
0.591MetTrp: 0.591 ± 0.165
0.322MetTyr: 0.322 ± 0.124
0.0MetXaa: 0.0 ± 0.0
Asn
3.707AsnAla: 3.707 ± 0.431
0.752AsnCys: 0.752 ± 0.207
1.558AsnAsp: 1.558 ± 0.254
1.988AsnGlu: 1.988 ± 0.312
0.699AsnPhe: 0.699 ± 0.171
4.137AsnGly: 4.137 ± 0.491
0.699AsnHis: 0.699 ± 0.212
1.29AsnIle: 1.29 ± 0.215
0.967AsnLys: 0.967 ± 0.201
2.74AsnLeu: 2.74 ± 0.41
0.645AsnMet: 0.645 ± 0.161
1.182AsnAsn: 1.182 ± 0.242
2.74AsnPro: 2.74 ± 0.396
0.752AsnGln: 0.752 ± 0.222
1.773AsnArg: 1.773 ± 0.351
1.773AsnSer: 1.773 ± 0.278
2.203AsnThr: 2.203 ± 0.327
1.881AsnVal: 1.881 ± 0.251
0.591AsnTrp: 0.591 ± 0.16
0.699AsnTyr: 0.699 ± 0.214
0.0AsnXaa: 0.0 ± 0.0
Pro
5.373ProAla: 5.373 ± 0.512
0.86ProCys: 0.86 ± 0.197
4.728ProAsp: 4.728 ± 0.489
5.212ProGlu: 5.212 ± 0.537
1.719ProPhe: 1.719 ± 0.245
5.749ProGly: 5.749 ± 0.642
1.397ProHis: 1.397 ± 0.229
2.203ProIle: 2.203 ± 0.345
2.257ProLys: 2.257 ± 0.374
3.17ProLeu: 3.17 ± 0.37
1.236ProMet: 1.236 ± 0.275
1.988ProAsn: 1.988 ± 0.349
3.331ProPro: 3.331 ± 0.588
1.881ProGln: 1.881 ± 0.343
3.009ProArg: 3.009 ± 0.426
2.579ProSer: 2.579 ± 0.377
3.761ProThr: 3.761 ± 0.534
4.191ProVal: 4.191 ± 0.575
1.236ProTrp: 1.236 ± 0.257
1.504ProTyr: 1.504 ± 0.252
0.0ProXaa: 0.0 ± 0.0
Gln
4.03GlnAla: 4.03 ± 0.406
0.591GlnCys: 0.591 ± 0.207
1.612GlnAsp: 1.612 ± 0.286
1.881GlnGlu: 1.881 ± 0.316
1.128GlnPhe: 1.128 ± 0.244
2.687GlnGly: 2.687 ± 0.322
1.021GlnHis: 1.021 ± 0.195
1.988GlnIle: 1.988 ± 0.276
1.504GlnLys: 1.504 ± 0.356
3.439GlnLeu: 3.439 ± 0.404
1.128GlnMet: 1.128 ± 0.261
1.397GlnAsn: 1.397 ± 0.274
2.31GlnPro: 2.31 ± 0.38
1.773GlnGln: 1.773 ± 0.339
2.794GlnArg: 2.794 ± 0.405
2.472GlnSer: 2.472 ± 0.293
1.558GlnThr: 1.558 ± 0.219
1.934GlnVal: 1.934 ± 0.336
0.699GlnTrp: 0.699 ± 0.209
1.182GlnTyr: 1.182 ± 0.265
0.0GlnXaa: 0.0 ± 0.0
Arg
5.91ArgAla: 5.91 ± 0.588
1.128ArgCys: 1.128 ± 0.321
4.299ArgAsp: 4.299 ± 0.5
4.406ArgGlu: 4.406 ± 0.479
2.042ArgPhe: 2.042 ± 0.355
4.084ArgGly: 4.084 ± 0.433
1.719ArgHis: 1.719 ± 0.33
3.439ArgIle: 3.439 ± 0.476
2.74ArgLys: 2.74 ± 0.457
5.749ArgLeu: 5.749 ± 0.639
1.934ArgMet: 1.934 ± 0.34
1.612ArgAsn: 1.612 ± 0.326
3.815ArgPro: 3.815 ± 0.577
2.687ArgGln: 2.687 ± 0.405
5.373ArgArg: 5.373 ± 0.792
3.654ArgSer: 3.654 ± 0.403
3.224ArgThr: 3.224 ± 0.489
4.943ArgVal: 4.943 ± 0.566
2.203ArgTrp: 2.203 ± 0.353
1.827ArgTyr: 1.827 ± 0.348
0.0ArgXaa: 0.0 ± 0.0
Ser
5.964SerAla: 5.964 ± 0.609
0.43SerCys: 0.43 ± 0.143
4.245SerAsp: 4.245 ± 0.395
2.902SerGlu: 2.902 ± 0.433
1.719SerPhe: 1.719 ± 0.355
6.716SerGly: 6.716 ± 0.971
1.182SerHis: 1.182 ± 0.285
2.74SerIle: 2.74 ± 0.359
1.827SerLys: 1.827 ± 0.331
4.191SerLeu: 4.191 ± 0.575
1.558SerMet: 1.558 ± 0.242
2.633SerAsn: 2.633 ± 0.346
2.902SerPro: 2.902 ± 0.353
1.934SerGln: 1.934 ± 0.276
4.03SerArg: 4.03 ± 0.545
3.707SerSer: 3.707 ± 0.657
3.815SerThr: 3.815 ± 0.435
4.137SerVal: 4.137 ± 0.654
1.182SerTrp: 1.182 ± 0.253
1.182SerTyr: 1.182 ± 0.251
0.0SerXaa: 0.0 ± 0.0
Thr
6.287ThrAla: 6.287 ± 0.605
0.645ThrCys: 0.645 ± 0.201
3.922ThrAsp: 3.922 ± 0.431
3.761ThrGlu: 3.761 ± 0.402
1.827ThrPhe: 1.827 ± 0.346
5.051ThrGly: 5.051 ± 0.799
1.128ThrHis: 1.128 ± 0.226
3.546ThrIle: 3.546 ± 0.598
1.29ThrLys: 1.29 ± 0.229
5.266ThrLeu: 5.266 ± 0.56
0.967ThrMet: 0.967 ± 0.224
2.418ThrAsn: 2.418 ± 0.388
4.997ThrPro: 4.997 ± 0.633
2.203ThrGln: 2.203 ± 0.435
3.815ThrArg: 3.815 ± 0.5
3.922ThrSer: 3.922 ± 0.538
3.546ThrThr: 3.546 ± 0.441
5.534ThrVal: 5.534 ± 0.652
1.666ThrTrp: 1.666 ± 0.342
0.86ThrTyr: 0.86 ± 0.249
0.0ThrXaa: 0.0 ± 0.0
Val
8.006ValAla: 8.006 ± 0.705
0.806ValCys: 0.806 ± 0.208
3.976ValAsp: 3.976 ± 0.539
5.158ValGlu: 5.158 ± 0.555
2.203ValPhe: 2.203 ± 0.357
5.105ValGly: 5.105 ± 0.555
1.827ValHis: 1.827 ± 0.352
3.116ValIle: 3.116 ± 0.489
2.418ValLys: 2.418 ± 0.357
5.749ValLeu: 5.749 ± 0.658
1.128ValMet: 1.128 ± 0.302
2.042ValAsn: 2.042 ± 0.344
4.03ValPro: 4.03 ± 0.367
2.203ValGln: 2.203 ± 0.296
4.352ValArg: 4.352 ± 0.527
4.836ValSer: 4.836 ± 0.782
4.621ValThr: 4.621 ± 0.717
5.373ValVal: 5.373 ± 0.705
1.881ValTrp: 1.881 ± 0.27
1.934ValTyr: 1.934 ± 0.36
0.0ValXaa: 0.0 ± 0.0
Trp
1.451TrpAla: 1.451 ± 0.28
0.645TrpCys: 0.645 ± 0.169
1.558TrpAsp: 1.558 ± 0.322
0.967TrpGlu: 0.967 ± 0.26
0.645TrpPhe: 0.645 ± 0.211
1.451TrpGly: 1.451 ± 0.338
0.322TrpHis: 0.322 ± 0.149
0.967TrpIle: 0.967 ± 0.251
1.182TrpLys: 1.182 ± 0.235
2.902TrpLeu: 2.902 ± 0.384
0.591TrpMet: 0.591 ± 0.161
0.645TrpAsn: 0.645 ± 0.178
1.128TrpPro: 1.128 ± 0.257
1.128TrpGln: 1.128 ± 0.216
1.397TrpArg: 1.397 ± 0.293
1.719TrpSer: 1.719 ± 0.305
1.558TrpThr: 1.558 ± 0.386
1.719TrpVal: 1.719 ± 0.386
0.913TrpTrp: 0.913 ± 0.262
0.699TrpTyr: 0.699 ± 0.165
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.988TyrAla: 1.988 ± 0.259
0.269TyrCys: 0.269 ± 0.13
1.719TyrAsp: 1.719 ± 0.266
1.504TyrGlu: 1.504 ± 0.244
0.752TyrPhe: 0.752 ± 0.233
2.364TyrGly: 2.364 ± 0.43
0.376TyrHis: 0.376 ± 0.145
0.967TyrIle: 0.967 ± 0.199
0.43TyrLys: 0.43 ± 0.145
1.988TyrLeu: 1.988 ± 0.37
0.376TyrMet: 0.376 ± 0.122
0.752TyrAsn: 0.752 ± 0.172
0.967TyrPro: 0.967 ± 0.217
0.913TyrGln: 0.913 ± 0.245
2.74TyrArg: 2.74 ± 0.431
1.128TyrSer: 1.128 ± 0.227
1.881TyrThr: 1.881 ± 0.292
1.773TyrVal: 1.773 ± 0.25
0.645TyrTrp: 0.645 ± 0.213
0.484TyrTyr: 0.484 ± 0.14
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 117 proteins (18612 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski