Sparus aurata (Gilthead sea bream)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei<

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69200 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A671VJU4|A0A671VJU4_SPAAU Isoform of A0A671VID7 BRCA1 interacting protein C-terminal helicase 1 OS=Sparus aurata OX=8175 GN=brip1 PE=4 SV=1
MM1 pKa = 7.6GVRR4 pKa = 11.84CINTSPGFRR13 pKa = 11.84CGSCPAGYY21 pKa = 8.41TGPQVQGVGLAYY33 pKa = 9.21ATANKK38 pKa = 9.26QVCKK42 pKa = 10.43DD43 pKa = 3.22INEE46 pKa = 4.55CSTSNGGCVEE56 pKa = 4.42NSVCMNTPGSFRR68 pKa = 11.84CGPCKK73 pKa = 9.86TGYY76 pKa = 10.67VGDD79 pKa = 3.78QRR81 pKa = 11.84RR82 pKa = 11.84GCKK85 pKa = 9.57PEE87 pKa = 3.83RR88 pKa = 11.84ACGNGQPNPCHH99 pKa = 6.71ASAEE103 pKa = 4.62CIVHH107 pKa = 6.61RR108 pKa = 11.84EE109 pKa = 3.94GKK111 pKa = 9.6IEE113 pKa = 4.14CQCGVGWAGNGYY125 pKa = 9.88LCGPDD130 pKa = 3.4SDD132 pKa = 4.6IDD134 pKa = 3.81GFPDD138 pKa = 3.68EE139 pKa = 5.77KK140 pKa = 10.84LACPDD145 pKa = 3.67TNCNKK150 pKa = 10.24DD151 pKa = 3.15NCLTVPNSGQEE162 pKa = 4.06DD163 pKa = 4.12ADD165 pKa = 3.79GDD167 pKa = 4.61GIGDD171 pKa = 4.07ACDD174 pKa = 3.53EE175 pKa = 4.74DD176 pKa = 5.69ADD178 pKa = 4.52GDD180 pKa = 4.74GILNMQDD187 pKa = 2.83NCVLVPNVDD196 pKa = 3.48QKK198 pKa = 10.85NTDD201 pKa = 3.25EE202 pKa = 5.68DD203 pKa = 4.59DD204 pKa = 5.05FGDD207 pKa = 3.87ACDD210 pKa = 3.29NCRR213 pKa = 11.84AVKK216 pKa = 10.88NNDD219 pKa = 3.3QKK221 pKa = 11.3DD222 pKa = 3.43TDD224 pKa = 3.37VDD226 pKa = 3.91KK227 pKa = 11.51FGDD230 pKa = 3.79EE231 pKa = 4.27CDD233 pKa = 3.68EE234 pKa = 5.81DD235 pKa = 4.18IDD237 pKa = 5.21GDD239 pKa = 4.45GIPNHH244 pKa = 7.08LDD246 pKa = 2.97NCKK249 pKa = 9.88RR250 pKa = 11.84VPNTDD255 pKa = 2.78QKK257 pKa = 11.7DD258 pKa = 3.53RR259 pKa = 11.84DD260 pKa = 3.6GDD262 pKa = 4.12KK263 pKa = 11.46VGDD266 pKa = 4.05ACDD269 pKa = 3.18SCPYY273 pKa = 10.08VKK275 pKa = 10.63NPEE278 pKa = 4.07QTDD281 pKa = 3.13VDD283 pKa = 3.7NDD285 pKa = 4.85LIGDD289 pKa = 3.96PCDD292 pKa = 3.62TNKK295 pKa = 10.93DD296 pKa = 3.49SDD298 pKa = 4.16GDD300 pKa = 3.81GHH302 pKa = 6.91QDD304 pKa = 4.2SRR306 pKa = 11.84DD307 pKa = 3.47NCPAVINSSQLDD319 pKa = 3.5TDD321 pKa = 3.78KK322 pKa = 11.37DD323 pKa = 4.07GKK325 pKa = 11.12GDD327 pKa = 3.71EE328 pKa = 5.3CDD330 pKa = 5.61DD331 pKa = 5.63DD332 pKa = 6.35DD333 pKa = 7.03DD334 pKa = 6.2NDD336 pKa = 5.44GIPDD340 pKa = 4.89LLPPGPDD347 pKa = 2.97NCRR350 pKa = 11.84LIPNPLQEE358 pKa = 4.62DD359 pKa = 4.3TNGDD363 pKa = 3.67GVGDD367 pKa = 3.54ICEE370 pKa = 4.37KK371 pKa = 11.1DD372 pKa = 3.39FDD374 pKa = 4.51NDD376 pKa = 4.38TIIDD380 pKa = 4.31TIDD383 pKa = 3.31VCPEE387 pKa = 3.65NAEE390 pKa = 4.04VTLTDD395 pKa = 3.59FRR397 pKa = 11.84EE398 pKa = 4.27YY399 pKa = 9.18QTVVLDD405 pKa = 4.15PEE407 pKa = 5.32GDD409 pKa = 3.65AQIDD413 pKa = 4.13PNWVVLNQYY422 pKa = 9.57IVLYY426 pKa = 9.86NSTSVTPVSPPGYY439 pKa = 7.78TAFSGVDD446 pKa = 3.72FEE448 pKa = 5.24GTFHH452 pKa = 6.81VNTVTDD458 pKa = 3.57DD459 pKa = 4.08DD460 pKa = 4.35YY461 pKa = 12.0AGFIFGYY468 pKa = 9.58QDD470 pKa = 2.83SSSFYY475 pKa = 9.91VVMWKK480 pKa = 9.52QVEE483 pKa = 4.18QIYY486 pKa = 8.33WQANPFRR493 pKa = 11.84AVAEE497 pKa = 4.08PGIQLKK503 pKa = 10.06AVKK506 pKa = 10.35SNTGPGEE513 pKa = 4.06NLRR516 pKa = 11.84NALWHH521 pKa = 6.38TGDD524 pKa = 3.08TSEE527 pKa = 5.12QVKK530 pKa = 10.14LLWKK534 pKa = 10.09DD535 pKa = 3.18ARR537 pKa = 11.84NVGWKK542 pKa = 10.41DD543 pKa = 3.0KK544 pKa = 10.83TSYY547 pKa = 10.52RR548 pKa = 11.84WFLQHH553 pKa = 6.87RR554 pKa = 11.84PADD557 pKa = 3.73GYY559 pKa = 10.45IRR561 pKa = 11.84VRR563 pKa = 11.84FYY565 pKa = 11.04EE566 pKa = 4.86GPQMVADD573 pKa = 3.78TGVIIDD579 pKa = 3.35ATMRR583 pKa = 11.84GGRR586 pKa = 11.84LGVFCFSQEE595 pKa = 3.95NIIWANLRR603 pKa = 11.84YY604 pKa = 9.71RR605 pKa = 11.84CNDD608 pKa = 3.48TLPEE612 pKa = 4.41DD613 pKa = 3.9FDD615 pKa = 3.96TYY617 pKa = 10.72RR618 pKa = 11.84AQQVQLVAA626 pKa = 4.4

Molecular weight: 68.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A671UXB6|A0A671UXB6_SPAAU 2 4-dienoyl-CoA reductase 1 OS=Sparus aurata OX=8175 GN=decr1 PE=4 SV=1
MM1 pKa = 6.64WTPGIVWTPRR11 pKa = 11.84IVIVWTPGIMWTLRR25 pKa = 11.84IVWTPGIVWTPGIMWTPRR43 pKa = 11.84IVWTPRR49 pKa = 11.84IMWTPGIVWTPGIMWTPGIVWTPRR73 pKa = 11.84IVWTPGIMWTLRR85 pKa = 11.84IVWTPGIVWTPGIMWTPRR103 pKa = 11.84IVWTPRR109 pKa = 11.84IMWTPGIVWTPGIMWIPGIVWTPRR133 pKa = 11.84IVVTPGIVGTPGIVWTPGIMWTPGIVWTPGIMWTPRR169 pKa = 11.84IVWTPGIMWTLRR181 pKa = 11.84IVWTPGIVWTPGIMWTLRR199 pKa = 11.84IVWTPGIVWTPGIMWTTRR217 pKa = 11.84IVGTPGIVGTPGIVWTPGIMWTPGIVWTPGIMWTPRR253 pKa = 11.84IVWTPGIVWTPGIMWTPGIVWTPGIMWTPRR283 pKa = 11.84IVWTPGIMWALRR295 pKa = 11.84IGWTPGIVWTPGIMWTLRR313 pKa = 11.84IVWTPGIVWTPGIMWTTRR331 pKa = 11.84IVGTPGIVGTPGIMWTPRR349 pKa = 11.84IVIGWTPGIVWTPGIMWTLRR369 pKa = 11.84IVWTPGIVWTPGIMWTTRR387 pKa = 11.84IMWTPGIVWTPGIMWTPRR405 pKa = 11.84IVWTPGIVWTPGIMWTPGIVWTPGIMWTPRR435 pKa = 11.84IVWTPGIMWTLRR447 pKa = 11.84IVWTPGIVWTPGIMWTLRR465 pKa = 11.84IVWTPGIVWTPGIMWTTRR483 pKa = 11.84IVGTPGIVGTPGIMWTPGIVWTPGIMWTPRR513 pKa = 11.84IVWTPGIVWTPGIMWTPGIVWTPGIMWTPRR543 pKa = 11.84IVWTPGIMWTLRR555 pKa = 11.84IVWTPGIVWTPRR567 pKa = 11.84IVWTPGIMWTPRR579 pKa = 11.84IVWTPGIMWTLRR591 pKa = 11.84IVWTPGIVWTPGIMWTLRR609 pKa = 11.84IVWTPGIVWTPGIMWTTRR627 pKa = 11.84IVGTPGIVGTPGIVWTPGIMWTPRR651 pKa = 11.84IVWTPGIMWTPRR663 pKa = 11.84IVWTPGIVWTPGIMWTPGIVWTPGIMWTPRR693 pKa = 11.84IVWTPGIMWTLRR705 pKa = 11.84IVWTPGIVWTPGIMWTLRR723 pKa = 11.84IVWTPGIVWTPGIMWTTRR741 pKa = 11.84IVGTPGIVGTPGIVVTPGIVWTPRR765 pKa = 11.84IVGLVCQHH773 pKa = 6.69SISSAIVLKK782 pKa = 10.67GHH784 pKa = 7.2LPGLVTGPTEE794 pKa = 4.17FMNSFHH800 pKa = 6.93SS801 pKa = 3.7

Molecular weight: 91.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

25145

44055

69200

47700975

16

8613

689.3

77.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.481 ± 0.007

2.299 ± 0.007

5.281 ± 0.006

6.808 ± 0.011

3.776 ± 0.006

6.279 ± 0.009

2.629 ± 0.004

4.595 ± 0.006

5.605 ± 0.01

9.653 ± 0.01

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.405 ± 0.004

3.899 ± 0.004

5.454 ± 0.009

4.659 ± 0.007

5.577 ± 0.007

8.357 ± 0.009

5.685 ± 0.009

6.496 ± 0.006

1.184 ± 0.003

2.87 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski