Phialophora americana

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Chaetothyriomycetidae; Chaetothyriales; Herpotrichiellaceae; Phialophora

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11503 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D2FUH5|A0A0D2FUH5_9EURO RNA helicase OS=Phialophora americana OX=5601 GN=PV04_08707 PE=4 SV=1
MM1 pKa = 7.16KK2 pKa = 10.19RR3 pKa = 11.84STRR6 pKa = 11.84AMPVAALLMAAANAQTTIINDD27 pKa = 3.87PADD30 pKa = 4.33PDD32 pKa = 3.65ATTVYY37 pKa = 10.99YY38 pKa = 7.95STCPTALTTTTTLSSTITFTCPGGEE63 pKa = 4.06CSGPTITPPPHH74 pKa = 6.53GSAQVSEE81 pKa = 4.21ILAFTTTLPDD91 pKa = 3.41GSVQEE96 pKa = 3.75IHH98 pKa = 6.95EE99 pKa = 4.96YY100 pKa = 8.35ITMYY104 pKa = 9.76PAIVSDD110 pKa = 4.46GSSIGQATYY119 pKa = 9.76TITEE123 pKa = 4.44ACPCEE128 pKa = 4.04ATRR131 pKa = 11.84RR132 pKa = 11.84PGHH135 pKa = 6.43IPDD138 pKa = 4.42GFTTTVTSCEE148 pKa = 4.31VCHH151 pKa = 6.62HH152 pKa = 6.95GGPTTMTMTVPCTTGPYY169 pKa = 9.24ATVTPHH175 pKa = 6.9IGPTGAAGGWGDD187 pKa = 3.5WDD189 pKa = 4.25GSGSDD194 pKa = 3.99PAEE197 pKa = 4.18SGAWGDD203 pKa = 3.53WDD205 pKa = 4.69GSGPDD210 pKa = 3.93PAKK213 pKa = 10.69SGAWGDD219 pKa = 3.78WNSADD224 pKa = 4.38PDD226 pKa = 4.23PAKK229 pKa = 10.75SGAWGDD235 pKa = 3.57WSSGADD241 pKa = 3.61PTKK244 pKa = 10.75SGAWGDD250 pKa = 3.85WNSADD255 pKa = 4.38PDD257 pKa = 4.23PAKK260 pKa = 10.75SGAWGDD266 pKa = 3.57WSSGADD272 pKa = 3.55PTATGAWGDD281 pKa = 3.78WKK283 pKa = 11.2SGDD286 pKa = 4.17PEE288 pKa = 4.08PTATGTWADD297 pKa = 3.64WEE299 pKa = 4.63GSDD302 pKa = 5.32PEE304 pKa = 4.26PTEE307 pKa = 4.31GGSWDD312 pKa = 4.0DD313 pKa = 3.15WDD315 pKa = 4.13KK316 pKa = 11.7SGSGGSGGSGSGSGGSGAGGASADD340 pKa = 3.27KK341 pKa = 9.91WDD343 pKa = 4.03NGSAGGGSPASPSVTPVSPGGVTPAQYY370 pKa = 10.3KK371 pKa = 10.47GSADD375 pKa = 3.2KK376 pKa = 11.45VGFAVSGVLAVAVGCLAFMLL396 pKa = 4.75

Molecular weight: 39.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D2F4Q3|A0A0D2F4Q3_9EURO Uncharacterized protein OS=Phialophora americana OX=5601 GN=PV04_09760 PE=4 SV=1
MM1 pKa = 7.16ATLARR6 pKa = 11.84VFQEE10 pKa = 3.78ACRR13 pKa = 11.84SRR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84DD18 pKa = 3.46RR19 pKa = 11.84VQHH22 pKa = 6.49ACACLPSLTRR32 pKa = 11.84SGEE35 pKa = 3.85LTATLKK41 pKa = 10.73RR42 pKa = 11.84RR43 pKa = 11.84GPPSTSFHH51 pKa = 6.25QISCRR56 pKa = 11.84SQPPGRR62 pKa = 11.84RR63 pKa = 11.84VKK65 pKa = 10.6SRR67 pKa = 11.84SASTSSGPSRR77 pKa = 11.84RR78 pKa = 11.84AVTVTSDD85 pKa = 3.18DD86 pKa = 3.3GRR88 pKa = 11.84YY89 pKa = 8.98NWSEE93 pKa = 3.74LSTGEE98 pKa = 3.67KK99 pKa = 9.89AARR102 pKa = 11.84GTQQTFNFLLVSLGLVGTITVAYY125 pKa = 9.56FLYY128 pKa = 10.59QEE130 pKa = 4.67LLAPSSSTIQFNAAVSRR147 pKa = 11.84IKK149 pKa = 10.81SSSEE153 pKa = 3.25CRR155 pKa = 11.84ALLGPSSQILAYY167 pKa = 10.32GEE169 pKa = 4.11PTSSKK174 pKa = 8.45WARR177 pKa = 11.84ARR179 pKa = 11.84PLAHH183 pKa = 5.68STEE186 pKa = 3.92VDD188 pKa = 3.46RR189 pKa = 11.84NGTTHH194 pKa = 7.0LRR196 pKa = 11.84MHH198 pKa = 6.77FNVEE202 pKa = 4.08GRR204 pKa = 11.84DD205 pKa = 3.59SGVKK209 pKa = 10.18GVVNVHH215 pKa = 4.62MTRR218 pKa = 11.84PKK220 pKa = 10.84DD221 pKa = 3.61GDD223 pKa = 3.47RR224 pKa = 11.84LEE226 pKa = 4.16YY227 pKa = 10.4QLLSLSVKK235 pKa = 9.71GHH237 pKa = 4.41EE238 pKa = 4.28TVYY241 pKa = 11.01LEE243 pKa = 4.04NKK245 pKa = 8.84AAEE248 pKa = 4.14RR249 pKa = 11.84GIKK252 pKa = 9.46GQAAKK257 pKa = 10.04MFGVQWRR264 pKa = 3.68

Molecular weight: 29.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10919

584

11503

5818011

55

6403

505.8

55.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.804 ± 0.019

1.21 ± 0.008

5.731 ± 0.016

6.048 ± 0.019

3.675 ± 0.012

6.843 ± 0.02

2.475 ± 0.01

4.732 ± 0.015

4.662 ± 0.019

8.95 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.15 ± 0.007

3.505 ± 0.012

6.203 ± 0.024

4.165 ± 0.017

6.324 ± 0.02

8.161 ± 0.026

6.039 ± 0.016

6.159 ± 0.016

1.454 ± 0.008

2.709 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski