Spiroplasma culicicola AES-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Entomoplasmatales; Spiroplasmataceae; Spiroplasma; Spiroplasma culicicola

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1071 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6A796|W6A796_9MOLU Uncharacterized protein OS=Spiroplasma culicicola AES-1 OX=1276246 GN=SCULI_v1c03640 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 9.31KK3 pKa = 8.56TVFSPEE9 pKa = 3.97DD10 pKa = 3.47FKK12 pKa = 11.65KK13 pKa = 11.16FEE15 pKa = 4.66DD16 pKa = 3.78NKK18 pKa = 10.8NIMMQIFGITCSVCGIDD35 pKa = 3.66EE36 pKa = 4.25IGYY39 pKa = 8.85VAQNAPKK46 pKa = 9.9TIGQIAQEE54 pKa = 4.04AMEE57 pKa = 4.85EE58 pKa = 4.21NPDD61 pKa = 3.44ITDD64 pKa = 3.48EE65 pKa = 4.12QLDD68 pKa = 4.0EE69 pKa = 5.04LMEE72 pKa = 4.43GPIGAWQEE80 pKa = 3.76VDD82 pKa = 4.96DD83 pKa = 4.4YY84 pKa = 10.88NASIGMPTFACDD96 pKa = 2.76NCYY99 pKa = 10.0QQLIDD104 pKa = 4.23GEE106 pKa = 4.43ISISDD111 pKa = 3.35ASDD114 pKa = 3.36QEE116 pKa = 4.37EE117 pKa = 4.22EE118 pKa = 4.1

Molecular weight: 13.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6A8T4|W6A8T4_9MOLU 50S ribosomal protein L29 OS=Spiroplasma culicicola AES-1 OX=1276246 GN=rpmC PE=3 SV=1
MM1 pKa = 7.32KK2 pKa = 9.47RR3 pKa = 11.84TWQPSKK9 pKa = 10.03IKK11 pKa = 10.14HH12 pKa = 5.53ARR14 pKa = 11.84THH16 pKa = 5.78GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.41NGRR28 pKa = 11.84KK29 pKa = 9.2VIKK32 pKa = 10.14ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.34GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1071

0

1071

360354

29

1760

336.5

38.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.333 ± 0.071

0.678 ± 0.017

5.509 ± 0.055

7.025 ± 0.073

5.476 ± 0.069

5.161 ± 0.076

1.316 ± 0.025

10.038 ± 0.084

9.16 ± 0.08

9.273 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.355 ± 0.032

7.267 ± 0.082

2.553 ± 0.039

3.561 ± 0.037

2.742 ± 0.048

6.191 ± 0.063

5.312 ± 0.05

5.799 ± 0.055

1.097 ± 0.028

4.155 ± 0.052
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski