Amino acid dipepetide frequency for Spiroplasma culicicola AES-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.266AlaAla: 3.266 ± 0.138
0.477AlaCys: 0.477 ± 0.036
2.27AlaAsp: 2.27 ± 0.08
2.708AlaGlu: 2.708 ± 0.105
2.631AlaPhe: 2.631 ± 0.083
3.111AlaGly: 3.111 ± 0.107
0.796AlaHis: 0.796 ± 0.06
5.692AlaIle: 5.692 ± 0.14
5.253AlaLys: 5.253 ± 0.136
5.092AlaLeu: 5.092 ± 0.161
1.285AlaMet: 1.285 ± 0.066
3.411AlaAsn: 3.411 ± 0.092
1.546AlaPro: 1.546 ± 0.095
2.09AlaGln: 2.09 ± 0.087
1.743AlaArg: 1.743 ± 0.076
3.286AlaSer: 3.286 ± 0.114
3.125AlaThr: 3.125 ± 0.117
3.072AlaVal: 3.072 ± 0.114
0.475AlaTrp: 0.475 ± 0.035
2.004AlaTyr: 2.004 ± 0.07
0.0AlaXaa: 0.0 ± 0.0
Cys
0.416CysAla: 0.416 ± 0.032
0.086CysCys: 0.086 ± 0.017
0.408CysAsp: 0.408 ± 0.036
0.455CysGlu: 0.455 ± 0.038
0.483CysPhe: 0.483 ± 0.039
0.511CysGly: 0.511 ± 0.039
0.125CysHis: 0.125 ± 0.022
0.541CysIle: 0.541 ± 0.037
0.458CysLys: 0.458 ± 0.042
0.702CysLeu: 0.702 ± 0.048
0.144CysMet: 0.144 ± 0.02
0.375CysAsn: 0.375 ± 0.033
0.189CysPro: 0.189 ± 0.024
0.25CysGln: 0.25 ± 0.025
0.158CysArg: 0.158 ± 0.022
0.441CysSer: 0.441 ± 0.037
0.311CysThr: 0.311 ± 0.03
0.358CysVal: 0.358 ± 0.032
0.108CysTrp: 0.108 ± 0.018
0.258CysTyr: 0.258 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
2.317AspAla: 2.317 ± 0.095
0.383AspCys: 0.383 ± 0.032
2.753AspAsp: 2.753 ± 0.107
4.199AspGlu: 4.199 ± 0.127
3.616AspPhe: 3.616 ± 0.116
2.647AspGly: 2.647 ± 0.1
0.819AspHis: 0.819 ± 0.045
5.672AspIle: 5.672 ± 0.124
4.787AspLys: 4.787 ± 0.137
5.742AspLeu: 5.742 ± 0.145
1.218AspMet: 1.218 ± 0.055
3.549AspAsn: 3.549 ± 0.103
1.676AspPro: 1.676 ± 0.072
2.226AspGln: 2.226 ± 0.07
1.343AspArg: 1.343 ± 0.066
3.272AspSer: 3.272 ± 0.108
2.198AspThr: 2.198 ± 0.073
3.211AspVal: 3.211 ± 0.11
0.722AspTrp: 0.722 ± 0.042
2.742AspTyr: 2.742 ± 0.1
0.0AspXaa: 0.0 ± 0.0
Glu
3.441GluAla: 3.441 ± 0.109
0.369GluCys: 0.369 ± 0.031
3.349GluAsp: 3.349 ± 0.115
5.37GluGlu: 5.37 ± 0.179
3.977GluPhe: 3.977 ± 0.121
2.958GluGly: 2.958 ± 0.096
1.052GluHis: 1.052 ± 0.055
7.806GluIle: 7.806 ± 0.192
6.552GluLys: 6.552 ± 0.183
6.668GluLeu: 6.668 ± 0.158
1.884GluMet: 1.884 ± 0.07
5.489GluAsn: 5.489 ± 0.138
1.493GluPro: 1.493 ± 0.059
3.386GluGln: 3.386 ± 0.119
1.976GluArg: 1.976 ± 0.083
3.261GluSer: 3.261 ± 0.1
3.363GluThr: 3.363 ± 0.109
4.168GluVal: 4.168 ± 0.117
0.71GluTrp: 0.71 ± 0.047
2.98GluTyr: 2.98 ± 0.104
0.0GluXaa: 0.0 ± 0.0
Phe
3.039PheAla: 3.039 ± 0.111
0.419PheCys: 0.419 ± 0.034
3.752PheAsp: 3.752 ± 0.112
4.121PheGlu: 4.121 ± 0.122
2.783PhePhe: 2.783 ± 0.135
3.164PheGly: 3.164 ± 0.119
0.491PheHis: 0.491 ± 0.039
5.386PheIle: 5.386 ± 0.165
5.001PheLys: 5.001 ± 0.137
4.776PheLeu: 4.776 ± 0.134
1.188PheMet: 1.188 ± 0.055
4.06PheAsn: 4.06 ± 0.116
1.027PhePro: 1.027 ± 0.054
1.321PheGln: 1.321 ± 0.068
1.279PheArg: 1.279 ± 0.058
3.938PheSer: 3.938 ± 0.113
2.93PheThr: 2.93 ± 0.106
3.266PheVal: 3.266 ± 0.098
0.611PheTrp: 0.611 ± 0.045
2.209PheTyr: 2.209 ± 0.081
0.0PheXaa: 0.0 ± 0.0
Gly
3.214GlyAla: 3.214 ± 0.124
0.408GlyCys: 0.408 ± 0.038
2.722GlyAsp: 2.722 ± 0.103
3.18GlyGlu: 3.18 ± 0.109
2.986GlyPhe: 2.986 ± 0.102
3.097GlyGly: 3.097 ± 0.134
0.769GlyHis: 0.769 ± 0.051
5.156GlyIle: 5.156 ± 0.141
3.991GlyLys: 3.991 ± 0.109
4.865GlyLeu: 4.865 ± 0.134
1.335GlyMet: 1.335 ± 0.061
2.689GlyAsn: 2.689 ± 0.099
1.168GlyPro: 1.168 ± 0.071
1.876GlyGln: 1.876 ± 0.072
1.543GlyArg: 1.543 ± 0.081
3.244GlySer: 3.244 ± 0.117
2.803GlyThr: 2.803 ± 0.099
3.447GlyVal: 3.447 ± 0.112
0.683GlyTrp: 0.683 ± 0.046
2.434GlyTyr: 2.434 ± 0.083
0.0GlyXaa: 0.0 ± 0.0
His
0.674HisAla: 0.674 ± 0.044
0.1HisCys: 0.1 ± 0.017
0.713HisAsp: 0.713 ± 0.046
0.83HisGlu: 0.83 ± 0.05
0.788HisPhe: 0.788 ± 0.045
0.735HisGly: 0.735 ± 0.052
0.247HisHis: 0.247 ± 0.023
1.29HisIle: 1.29 ± 0.053
1.018HisLys: 1.018 ± 0.06
1.29HisLeu: 1.29 ± 0.058
0.297HisMet: 0.297 ± 0.031
0.927HisAsn: 0.927 ± 0.059
0.477HisPro: 0.477 ± 0.038
0.502HisGln: 0.502 ± 0.035
0.389HisArg: 0.389 ± 0.037
0.752HisSer: 0.752 ± 0.046
0.611HisThr: 0.611 ± 0.044
0.771HisVal: 0.771 ± 0.044
0.167HisTrp: 0.167 ± 0.021
0.583HisTyr: 0.583 ± 0.04
0.0HisXaa: 0.0 ± 0.0
Ile
6.088IleAla: 6.088 ± 0.152
0.93IleCys: 0.93 ± 0.053
6.385IleAsp: 6.385 ± 0.146
7.387IleGlu: 7.387 ± 0.158
5.384IlePhe: 5.384 ± 0.187
5.27IleGly: 5.27 ± 0.162
1.107IleHis: 1.107 ± 0.055
9.455IleIle: 9.455 ± 0.199
9.396IleLys: 9.396 ± 0.189
8.866IleLeu: 8.866 ± 0.18
2.112IleMet: 2.112 ± 0.08
7.643IleAsn: 7.643 ± 0.156
2.531IlePro: 2.531 ± 0.089
2.697IleGln: 2.697 ± 0.079
2.503IleArg: 2.503 ± 0.087
6.655IleSer: 6.655 ± 0.166
5.181IleThr: 5.181 ± 0.122
5.958IleVal: 5.958 ± 0.145
0.871IleTrp: 0.871 ± 0.048
3.963IleTyr: 3.963 ± 0.135
0.0IleXaa: 0.0 ± 0.0
Lys
4.534LysAla: 4.534 ± 0.15
0.352LysCys: 0.352 ± 0.035
5.284LysAsp: 5.284 ± 0.123
7.862LysGlu: 7.862 ± 0.164
4.027LysPhe: 4.027 ± 0.119
3.638LysGly: 3.638 ± 0.123
1.254LysHis: 1.254 ± 0.058
8.875LysIle: 8.875 ± 0.199
8.68LysLys: 8.68 ± 0.166
7.831LysLeu: 7.831 ± 0.173
2.606LysMet: 2.606 ± 0.08
7.379LysAsn: 7.379 ± 0.159
2.276LysPro: 2.276 ± 0.085
3.599LysGln: 3.599 ± 0.114
2.867LysArg: 2.867 ± 0.099
4.623LysSer: 4.623 ± 0.112
5.417LysThr: 5.417 ± 0.122
5.381LysVal: 5.381 ± 0.125
0.944LysTrp: 0.944 ± 0.051
4.165LysTyr: 4.165 ± 0.119
0.0LysXaa: 0.0 ± 0.0
Leu
5.012LeuAla: 5.012 ± 0.133
0.552LeuCys: 0.552 ± 0.034
5.25LeuAsp: 5.25 ± 0.132
6.696LeuGlu: 6.696 ± 0.144
5.164LeuPhe: 5.164 ± 0.167
4.637LeuGly: 4.637 ± 0.144
1.063LeuHis: 1.063 ± 0.044
9.369LeuIle: 9.369 ± 0.201
9.099LeuLys: 9.099 ± 0.176
8.098LeuLeu: 8.098 ± 0.179
2.22LeuMet: 2.22 ± 0.085
7.537LeuAsn: 7.537 ± 0.151
2.284LeuPro: 2.284 ± 0.08
2.714LeuGln: 2.714 ± 0.09
2.436LeuArg: 2.436 ± 0.085
6.011LeuSer: 6.011 ± 0.145
5.475LeuThr: 5.475 ± 0.13
5.12LeuVal: 5.12 ± 0.135
0.946LeuTrp: 0.946 ± 0.053
3.041LeuTyr: 3.041 ± 0.1
0.0LeuXaa: 0.0 ± 0.0
Met
1.543MetAla: 1.543 ± 0.072
0.147MetCys: 0.147 ± 0.022
1.141MetAsp: 1.141 ± 0.052
1.202MetGlu: 1.202 ± 0.054
1.374MetPhe: 1.374 ± 0.064
1.371MetGly: 1.371 ± 0.068
0.314MetHis: 0.314 ± 0.032
2.201MetIle: 2.201 ± 0.079
2.303MetLys: 2.303 ± 0.081
2.178MetLeu: 2.178 ± 0.068
0.574MetMet: 0.574 ± 0.043
1.61MetAsn: 1.61 ± 0.071
0.821MetPro: 0.821 ± 0.051
0.827MetGln: 0.827 ± 0.049
0.605MetArg: 0.605 ± 0.045
1.637MetSer: 1.637 ± 0.068
1.213MetThr: 1.213 ± 0.058
1.404MetVal: 1.404 ± 0.059
0.228MetTrp: 0.228 ± 0.023
0.857MetTyr: 0.857 ± 0.052
0.0MetXaa: 0.0 ± 0.0
Asn
3.208AsnAla: 3.208 ± 0.1
0.505AsnCys: 0.505 ± 0.038
4.243AsnAsp: 4.243 ± 0.113
5.092AsnGlu: 5.092 ± 0.12
4.049AsnPhe: 4.049 ± 0.116
3.497AsnGly: 3.497 ± 0.101
0.935AsnHis: 0.935 ± 0.051
7.265AsnIle: 7.265 ± 0.142
6.835AsnLys: 6.835 ± 0.133
6.799AsnLeu: 6.799 ± 0.158
1.426AsnMet: 1.426 ± 0.061
5.819AsnAsn: 5.819 ± 0.154
2.19AsnPro: 2.19 ± 0.082
3.194AsnGln: 3.194 ± 0.097
1.701AsnArg: 1.701 ± 0.064
4.651AsnSer: 4.651 ± 0.139
3.144AsnThr: 3.144 ± 0.105
3.796AsnVal: 3.796 ± 0.095
0.877AsnTrp: 0.877 ± 0.047
3.441AsnTyr: 3.441 ± 0.118
0.0AsnXaa: 0.0 ± 0.0
Pro
1.254ProAla: 1.254 ± 0.077
0.136ProCys: 0.136 ± 0.02
1.304ProAsp: 1.304 ± 0.059
2.101ProGlu: 2.101 ± 0.083
1.382ProPhe: 1.382 ± 0.06
1.507ProGly: 1.507 ± 0.072
0.336ProHis: 0.336 ± 0.035
2.634ProIle: 2.634 ± 0.095
2.056ProLys: 2.056 ± 0.083
2.337ProLeu: 2.337 ± 0.086
0.611ProMet: 0.611 ± 0.051
1.665ProAsn: 1.665 ± 0.069
0.472ProPro: 0.472 ± 0.037
0.938ProGln: 0.938 ± 0.058
0.647ProArg: 0.647 ± 0.042
1.573ProSer: 1.573 ± 0.078
1.654ProThr: 1.654 ± 0.064
1.637ProVal: 1.637 ± 0.073
0.294ProTrp: 0.294 ± 0.026
0.993ProTyr: 0.993 ± 0.056
0.0ProXaa: 0.0 ± 0.0
Gln
1.826GlnAla: 1.826 ± 0.071
0.119GlnCys: 0.119 ± 0.017
1.748GlnAsp: 1.748 ± 0.073
3.008GlnGlu: 3.008 ± 0.094
1.812GlnPhe: 1.812 ± 0.065
1.396GlnGly: 1.396 ± 0.061
0.397GlnHis: 0.397 ± 0.033
3.683GlnIle: 3.683 ± 0.098
3.721GlnLys: 3.721 ± 0.115
3.513GlnLeu: 3.513 ± 0.11
0.835GlnMet: 0.835 ± 0.047
2.75GlnAsn: 2.75 ± 0.093
0.755GlnPro: 0.755 ± 0.047
1.368GlnGln: 1.368 ± 0.071
1.102GlnArg: 1.102 ± 0.06
1.845GlnSer: 1.845 ± 0.079
1.848GlnThr: 1.848 ± 0.071
1.956GlnVal: 1.956 ± 0.074
0.391GlnTrp: 0.391 ± 0.034
1.54GlnTyr: 1.54 ± 0.061
0.0GlnXaa: 0.0 ± 0.0
Arg
1.493ArgAla: 1.493 ± 0.071
0.158ArgCys: 0.158 ± 0.021
1.535ArgAsp: 1.535 ± 0.06
1.934ArgGlu: 1.934 ± 0.078
1.479ArgPhe: 1.479 ± 0.063
1.374ArgGly: 1.374 ± 0.058
0.452ArgHis: 0.452 ± 0.036
2.85ArgIle: 2.85 ± 0.094
2.434ArgLys: 2.434 ± 0.076
2.209ArgLeu: 2.209 ± 0.091
0.788ArgMet: 0.788 ± 0.049
1.99ArgAsn: 1.99 ± 0.077
0.741ArgPro: 0.741 ± 0.051
1.016ArgGln: 1.016 ± 0.058
0.985ArgArg: 0.985 ± 0.06
1.449ArgSer: 1.449 ± 0.069
1.462ArgThr: 1.462 ± 0.073
1.526ArgVal: 1.526 ± 0.064
0.291ArgTrp: 0.291 ± 0.027
1.252ArgTyr: 1.252 ± 0.062
0.0ArgXaa: 0.0 ± 0.0
Ser
3.039SerAla: 3.039 ± 0.119
0.372SerCys: 0.372 ± 0.034
2.944SerAsp: 2.944 ± 0.097
3.571SerGlu: 3.571 ± 0.106
3.646SerPhe: 3.646 ± 0.132
3.477SerGly: 3.477 ± 0.103
0.852SerHis: 0.852 ± 0.049
6.388SerIle: 6.388 ± 0.149
5.708SerLys: 5.708 ± 0.126
5.991SerLeu: 5.991 ± 0.135
1.385SerMet: 1.385 ± 0.068
4.276SerAsn: 4.276 ± 0.128
1.351SerPro: 1.351 ± 0.061
2.356SerGln: 2.356 ± 0.099
1.723SerArg: 1.723 ± 0.075
4.496SerSer: 4.496 ± 0.132
3.61SerThr: 3.61 ± 0.122
3.427SerVal: 3.427 ± 0.098
0.719SerTrp: 0.719 ± 0.048
2.581SerTyr: 2.581 ± 0.082
0.0SerXaa: 0.0 ± 0.0
Thr
2.747ThrAla: 2.747 ± 0.103
0.333ThrCys: 0.333 ± 0.03
2.584ThrAsp: 2.584 ± 0.096
2.711ThrGlu: 2.711 ± 0.094
2.917ThrPhe: 2.917 ± 0.1
3.394ThrGly: 3.394 ± 0.102
0.694ThrHis: 0.694 ± 0.046
5.794ThrIle: 5.794 ± 0.125
4.526ThrLys: 4.526 ± 0.105
5.17ThrLeu: 5.17 ± 0.133
1.11ThrMet: 1.11 ± 0.057
3.707ThrAsn: 3.707 ± 0.098
1.679ThrPro: 1.679 ± 0.072
1.687ThrGln: 1.687 ± 0.068
1.44ThrArg: 1.44 ± 0.062
3.61ThrSer: 3.61 ± 0.115
3.15ThrThr: 3.15 ± 0.123
3.189ThrVal: 3.189 ± 0.109
0.547ThrTrp: 0.547 ± 0.039
2.128ThrTyr: 2.128 ± 0.085
0.0ThrXaa: 0.0 ± 0.0
Val
3.61ValAla: 3.61 ± 0.112
0.486ValCys: 0.486 ± 0.037
3.552ValAsp: 3.552 ± 0.103
4.451ValGlu: 4.451 ± 0.148
2.958ValPhe: 2.958 ± 0.091
3.288ValGly: 3.288 ± 0.115
0.755ValHis: 0.755 ± 0.051
5.417ValIle: 5.417 ± 0.14
5.092ValLys: 5.092 ± 0.119
5.212ValLeu: 5.212 ± 0.126
1.371ValMet: 1.371 ± 0.054
3.938ValAsn: 3.938 ± 0.115
1.568ValPro: 1.568 ± 0.078
1.515ValGln: 1.515 ± 0.067
1.535ValArg: 1.535 ± 0.068
3.705ValSer: 3.705 ± 0.099
3.061ValThr: 3.061 ± 0.112
3.563ValVal: 3.563 ± 0.121
0.591ValTrp: 0.591 ± 0.039
2.325ValTyr: 2.325 ± 0.083
0.0ValXaa: 0.0 ± 0.0
Trp
0.549TrpAla: 0.549 ± 0.043
0.056TrpCys: 0.056 ± 0.012
0.605TrpAsp: 0.605 ± 0.038
0.597TrpGlu: 0.597 ± 0.043
0.688TrpPhe: 0.688 ± 0.045
0.472TrpGly: 0.472 ± 0.04
0.122TrpHis: 0.122 ± 0.019
1.141TrpIle: 1.141 ± 0.066
0.963TrpLys: 0.963 ± 0.052
1.107TrpLeu: 1.107 ± 0.056
0.364TrpMet: 0.364 ± 0.034
0.71TrpAsn: 0.71 ± 0.047
0.244TrpPro: 0.244 ± 0.024
0.402TrpGln: 0.402 ± 0.033
0.241TrpArg: 0.241 ± 0.027
0.727TrpSer: 0.727 ± 0.044
0.635TrpThr: 0.635 ± 0.044
0.627TrpVal: 0.627 ± 0.048
0.23TrpTrp: 0.23 ± 0.026
0.486TrpTyr: 0.486 ± 0.04
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.059TyrAla: 2.059 ± 0.08
0.389TyrCys: 0.389 ± 0.029
2.548TyrAsp: 2.548 ± 0.094
2.778TyrGlu: 2.778 ± 0.09
2.617TyrPhe: 2.617 ± 0.095
2.065TyrGly: 2.065 ± 0.074
0.583TyrHis: 0.583 ± 0.037
3.769TyrIle: 3.769 ± 0.112
3.721TyrLys: 3.721 ± 0.113
4.279TyrLeu: 4.279 ± 0.137
0.794TyrMet: 0.794 ± 0.051
3.144TyrAsn: 3.144 ± 0.097
1.043TyrPro: 1.043 ± 0.056
1.651TyrGln: 1.651 ± 0.071
1.202TyrArg: 1.202 ± 0.064
2.739TyrSer: 2.739 ± 0.095
1.926TyrThr: 1.926 ± 0.074
2.115TyrVal: 2.115 ± 0.075
0.563TyrTrp: 0.563 ± 0.041
1.562TyrTyr: 1.562 ± 0.07
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1071 proteins (360354 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski