Psilocybe cyanescens

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Strophariaceae; Psilocybe

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15931 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A409XBD9|A0A409XBD9_PSICY GMC_OxRdtase_N domain-containing protein OS=Psilocybe cyanescens OX=93625 GN=CVT25_000843 PE=3 SV=1
MM1 pKa = 6.99TWRR4 pKa = 11.84TAEE7 pKa = 4.16TLAHH11 pKa = 6.86DD12 pKa = 4.16CQFAHH17 pKa = 6.4SASVLIDD24 pKa = 3.41GSFLGPITHH33 pKa = 6.6HH34 pKa = 5.57TAISIDD40 pKa = 3.64NQLALTLGFVSTAAAWNSLSDD61 pKa = 4.03EE62 pKa = 4.82DD63 pKa = 5.11EE64 pKa = 4.93DD65 pKa = 6.33DD66 pKa = 3.99NFIPADD72 pKa = 3.7ALPTNKK78 pKa = 10.13ASTNPPLSIAAQVAEE93 pKa = 4.12LWLRR97 pKa = 11.84DD98 pKa = 3.55SSPEE102 pKa = 3.79VSSPSPQHH110 pKa = 6.19NFHH113 pKa = 6.78TGPNDD118 pKa = 3.82LAVAWAISSDD128 pKa = 3.54EE129 pKa = 4.46DD130 pKa = 3.84LASDD134 pKa = 4.32GAYY137 pKa = 8.74PNSVISIEE145 pKa = 4.28AVCHH149 pKa = 5.17VLSHH153 pKa = 6.57EE154 pKa = 4.28NLNILASVGATDD166 pKa = 5.88AGAVWLNSLDD176 pKa = 3.76SGANNSLGPAAEE188 pKa = 4.16QQYY191 pKa = 8.26STIFPNPITPPVGLAEE207 pKa = 4.3LTVVWYY213 pKa = 10.83SSDD216 pKa = 3.6KK217 pKa = 11.05DD218 pKa = 3.54QSSYY222 pKa = 11.03SASAPPSDD230 pKa = 3.12WHH232 pKa = 6.93LSQADD237 pKa = 3.45SSMRR241 pKa = 11.84PAQVYY246 pKa = 7.66MAVDD250 pKa = 3.92SVTPSHH256 pKa = 6.38ITLPCPFTPTFFDD269 pKa = 4.48FVDD272 pKa = 4.2DD273 pKa = 3.45TWLPAEE279 pKa = 4.7EE280 pKa = 5.49DD281 pKa = 3.55SGDD284 pKa = 3.9SNNEE288 pKa = 2.92QWAADD293 pKa = 3.67TDD295 pKa = 3.94DD296 pKa = 3.73TEE298 pKa = 4.74EE299 pKa = 4.04FVLL302 pKa = 5.13

Molecular weight: 32.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A409WEU8|A0A409WEU8_PSICY Uncharacterized protein OS=Psilocybe cyanescens OX=93625 GN=CVT25_014831 PE=4 SV=1
MM1 pKa = 7.44RR2 pKa = 11.84QRR4 pKa = 11.84TRR6 pKa = 11.84MTAMATPMRR15 pKa = 11.84TTAMTRR21 pKa = 11.84SWTRR25 pKa = 11.84MTATATTRR33 pKa = 11.84TRR35 pKa = 11.84TRR37 pKa = 11.84TTTRR41 pKa = 11.84TARR44 pKa = 11.84ARR46 pKa = 11.84TTAA49 pKa = 3.37

Molecular weight: 5.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15931

0

15931

6841066

16

5056

429.4

47.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.129 ± 0.019

1.211 ± 0.007

5.557 ± 0.015

5.682 ± 0.02

3.818 ± 0.013

6.354 ± 0.019

2.607 ± 0.01

5.307 ± 0.017

4.71 ± 0.019

9.128 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.081 ± 0.007

3.89 ± 0.011

6.354 ± 0.025

3.822 ± 0.014

5.755 ± 0.017

9.111 ± 0.02

6.221 ± 0.013

6.185 ± 0.015

1.358 ± 0.006

2.713 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski