Amino acid dipepetide frequency for Proteus phage PM87

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.408AlaAla: 3.408 ± 0.692
0.646AlaCys: 0.646 ± 0.209
4.583AlaAsp: 4.583 ± 0.608
5.641AlaGlu: 5.641 ± 0.698
3.643AlaPhe: 3.643 ± 0.455
4.877AlaGly: 4.877 ± 0.489
0.999AlaHis: 0.999 ± 0.217
4.29AlaIle: 4.29 ± 0.662
5.935AlaLys: 5.935 ± 1.046
5.759AlaLeu: 5.759 ± 0.745
2.468AlaMet: 2.468 ± 0.432
3.467AlaAsn: 3.467 ± 0.538
2.879AlaPro: 2.879 ± 0.522
2.644AlaGln: 2.644 ± 0.487
4.466AlaArg: 4.466 ± 0.679
6.052AlaSer: 6.052 ± 0.751
4.995AlaThr: 4.995 ± 0.573
4.818AlaVal: 4.818 ± 0.685
1.645AlaTrp: 1.645 ± 0.326
2.409AlaTyr: 2.409 ± 0.424
0.0AlaXaa: 0.0 ± 0.0
Cys
0.588CysAla: 0.588 ± 0.169
0.0CysCys: 0.0 ± 0.0
0.823CysAsp: 0.823 ± 0.253
0.705CysGlu: 0.705 ± 0.237
0.411CysPhe: 0.411 ± 0.155
0.999CysGly: 0.999 ± 0.258
0.118CysHis: 0.118 ± 0.085
0.353CysIle: 0.353 ± 0.148
0.353CysLys: 0.353 ± 0.132
0.529CysLeu: 0.529 ± 0.182
0.353CysMet: 0.353 ± 0.156
0.411CysAsn: 0.411 ± 0.181
0.823CysPro: 0.823 ± 0.254
0.235CysGln: 0.235 ± 0.16
0.588CysArg: 0.588 ± 0.185
0.235CysSer: 0.235 ± 0.106
0.353CysThr: 0.353 ± 0.121
0.705CysVal: 0.705 ± 0.222
0.059CysTrp: 0.059 ± 0.057
0.353CysTyr: 0.353 ± 0.133
0.0CysXaa: 0.0 ± 0.0
Asp
4.818AspAla: 4.818 ± 0.514
0.705AspCys: 0.705 ± 0.198
4.055AspAsp: 4.055 ± 0.605
4.936AspGlu: 4.936 ± 0.531
2.35AspPhe: 2.35 ± 0.457
6.052AspGly: 6.052 ± 0.856
1.058AspHis: 1.058 ± 0.262
3.996AspIle: 3.996 ± 0.615
4.113AspLys: 4.113 ± 0.544
5.582AspLeu: 5.582 ± 0.567
2.057AspMet: 2.057 ± 0.466
3.056AspAsn: 3.056 ± 0.433
2.997AspPro: 2.997 ± 0.663
1.41AspGln: 1.41 ± 0.299
3.173AspArg: 3.173 ± 0.42
3.291AspSer: 3.291 ± 0.461
3.643AspThr: 3.643 ± 0.486
4.701AspVal: 4.701 ± 0.542
1.175AspTrp: 1.175 ± 0.277
1.88AspTyr: 1.88 ± 0.354
0.0AspXaa: 0.0 ± 0.0
Glu
5.289GluAla: 5.289 ± 0.748
0.588GluCys: 0.588 ± 0.21
4.348GluAsp: 4.348 ± 0.588
5.817GluGlu: 5.817 ± 0.626
2.527GluPhe: 2.527 ± 0.338
2.762GluGly: 2.762 ± 0.374
0.881GluHis: 0.881 ± 0.27
4.877GluIle: 4.877 ± 0.525
4.818GluLys: 4.818 ± 0.551
5.994GluLeu: 5.994 ± 0.684
1.998GluMet: 1.998 ± 0.313
3.526GluAsn: 3.526 ± 0.452
2.409GluPro: 2.409 ± 0.421
2.292GluGln: 2.292 ± 0.532
3.584GluArg: 3.584 ± 0.517
4.583GluSer: 4.583 ± 0.638
5.112GluThr: 5.112 ± 0.557
4.936GluVal: 4.936 ± 0.411
1.469GluTrp: 1.469 ± 0.286
2.174GluTyr: 2.174 ± 0.355
0.0GluXaa: 0.0 ± 0.0
Phe
2.821PheAla: 2.821 ± 0.32
0.646PheCys: 0.646 ± 0.189
3.114PheAsp: 3.114 ± 0.475
4.348PheGlu: 4.348 ± 0.512
1.763PhePhe: 1.763 ± 0.329
3.526PheGly: 3.526 ± 0.455
0.588PheHis: 0.588 ± 0.212
2.644PheIle: 2.644 ± 0.346
2.468PheLys: 2.468 ± 0.406
2.703PheLeu: 2.703 ± 0.391
0.823PheMet: 0.823 ± 0.222
1.704PheAsn: 1.704 ± 0.265
1.293PhePro: 1.293 ± 0.305
1.352PheGln: 1.352 ± 0.354
2.057PheArg: 2.057 ± 0.307
2.35PheSer: 2.35 ± 0.403
2.762PheThr: 2.762 ± 0.442
2.292PheVal: 2.292 ± 0.313
0.294PheTrp: 0.294 ± 0.114
1.116PheTyr: 1.116 ± 0.204
0.0PheXaa: 0.0 ± 0.0
Gly
4.407GlyAla: 4.407 ± 0.581
1.058GlyCys: 1.058 ± 0.3
4.466GlyAsp: 4.466 ± 0.646
5.053GlyGlu: 5.053 ± 0.516
3.232GlyPhe: 3.232 ± 0.465
5.524GlyGly: 5.524 ± 0.896
0.999GlyHis: 0.999 ± 0.224
4.348GlyIle: 4.348 ± 0.611
5.053GlyLys: 5.053 ± 0.641
4.525GlyLeu: 4.525 ± 0.529
1.88GlyMet: 1.88 ± 0.392
2.115GlyAsn: 2.115 ± 0.383
1.469GlyPro: 1.469 ± 0.227
3.349GlyGln: 3.349 ± 0.413
4.583GlyArg: 4.583 ± 0.544
4.995GlySer: 4.995 ± 0.573
3.996GlyThr: 3.996 ± 0.481
6.111GlyVal: 6.111 ± 0.533
1.116GlyTrp: 1.116 ± 0.316
3.114GlyTyr: 3.114 ± 0.469
0.0GlyXaa: 0.0 ± 0.0
His
1.175HisAla: 1.175 ± 0.212
0.059HisCys: 0.059 ± 0.051
1.058HisAsp: 1.058 ± 0.255
0.705HisGlu: 0.705 ± 0.154
0.411HisPhe: 0.411 ± 0.156
0.999HisGly: 0.999 ± 0.213
0.294HisHis: 0.294 ± 0.115
0.94HisIle: 0.94 ± 0.246
0.823HisLys: 0.823 ± 0.208
1.704HisLeu: 1.704 ± 0.254
0.47HisMet: 0.47 ± 0.195
0.529HisAsn: 0.529 ± 0.158
0.881HisPro: 0.881 ± 0.175
0.353HisGln: 0.353 ± 0.136
1.645HisArg: 1.645 ± 0.321
0.823HisSer: 0.823 ± 0.194
0.764HisThr: 0.764 ± 0.209
1.175HisVal: 1.175 ± 0.226
0.176HisTrp: 0.176 ± 0.11
0.529HisTyr: 0.529 ± 0.211
0.0HisXaa: 0.0 ± 0.0
Ile
4.466IleAla: 4.466 ± 0.448
0.529IleCys: 0.529 ± 0.211
4.231IleAsp: 4.231 ± 0.489
4.877IleGlu: 4.877 ± 0.657
1.88IlePhe: 1.88 ± 0.263
3.526IleGly: 3.526 ± 0.524
1.41IleHis: 1.41 ± 0.271
3.526IleIle: 3.526 ± 0.366
4.701IleLys: 4.701 ± 0.518
3.878IleLeu: 3.878 ± 0.435
1.175IleMet: 1.175 ± 0.242
3.526IleAsn: 3.526 ± 0.555
3.114IlePro: 3.114 ± 0.401
1.469IleGln: 1.469 ± 0.361
3.408IleArg: 3.408 ± 0.4
4.76IleSer: 4.76 ± 0.502
4.055IleThr: 4.055 ± 0.356
3.173IleVal: 3.173 ± 0.37
0.294IleTrp: 0.294 ± 0.116
1.998IleTyr: 1.998 ± 0.35
0.0IleXaa: 0.0 ± 0.0
Lys
5.641LysAla: 5.641 ± 0.803
0.411LysCys: 0.411 ± 0.167
3.996LysAsp: 3.996 ± 0.474
4.172LysGlu: 4.172 ± 0.488
2.468LysPhe: 2.468 ± 0.289
3.702LysGly: 3.702 ± 0.386
1.116LysHis: 1.116 ± 0.265
3.173LysIle: 3.173 ± 0.511
4.113LysLys: 4.113 ± 0.566
4.995LysLeu: 4.995 ± 0.667
2.115LysMet: 2.115 ± 0.4
2.762LysAsn: 2.762 ± 0.401
3.526LysPro: 3.526 ± 0.488
1.704LysGln: 1.704 ± 0.351
3.467LysArg: 3.467 ± 0.63
4.172LysSer: 4.172 ± 0.54
3.819LysThr: 3.819 ± 0.454
3.996LysVal: 3.996 ± 0.63
0.999LysTrp: 0.999 ± 0.353
2.057LysTyr: 2.057 ± 0.365
0.0LysXaa: 0.0 ± 0.0
Leu
6.816LeuAla: 6.816 ± 0.77
0.705LeuCys: 0.705 ± 0.203
4.936LeuAsp: 4.936 ± 0.688
4.525LeuGlu: 4.525 ± 0.498
2.35LeuPhe: 2.35 ± 0.375
5.347LeuGly: 5.347 ± 0.598
0.881LeuHis: 0.881 ± 0.233
3.702LeuIle: 3.702 ± 0.509
5.053LeuLys: 5.053 ± 0.612
5.171LeuLeu: 5.171 ± 0.479
2.115LeuMet: 2.115 ± 0.388
3.878LeuAsn: 3.878 ± 0.449
3.643LeuPro: 3.643 ± 0.54
2.115LeuGln: 2.115 ± 0.355
5.171LeuArg: 5.171 ± 0.517
5.7LeuSer: 5.7 ± 0.601
5.23LeuThr: 5.23 ± 0.572
5.759LeuVal: 5.759 ± 0.477
0.411LeuTrp: 0.411 ± 0.166
1.998LeuTyr: 1.998 ± 0.287
0.0LeuXaa: 0.0 ± 0.0
Met
3.526MetAla: 3.526 ± 0.444
0.118MetCys: 0.118 ± 0.082
1.116MetAsp: 1.116 ± 0.287
1.41MetGlu: 1.41 ± 0.301
0.705MetPhe: 0.705 ± 0.219
1.587MetGly: 1.587 ± 0.298
0.353MetHis: 0.353 ± 0.122
1.763MetIle: 1.763 ± 0.296
1.528MetLys: 1.528 ± 0.25
2.409MetLeu: 2.409 ± 0.298
0.646MetMet: 0.646 ± 0.183
1.469MetAsn: 1.469 ± 0.232
1.293MetPro: 1.293 ± 0.282
0.47MetGln: 0.47 ± 0.201
1.704MetArg: 1.704 ± 0.332
1.704MetSer: 1.704 ± 0.351
1.763MetThr: 1.763 ± 0.287
2.174MetVal: 2.174 ± 0.324
0.411MetTrp: 0.411 ± 0.14
0.823MetTyr: 0.823 ± 0.188
0.0MetXaa: 0.0 ± 0.0
Asn
4.348AsnAla: 4.348 ± 0.802
0.353AsnCys: 0.353 ± 0.182
2.585AsnAsp: 2.585 ± 0.37
2.762AsnGlu: 2.762 ± 0.349
1.587AsnPhe: 1.587 ± 0.3
4.407AsnGly: 4.407 ± 0.451
0.529AsnHis: 0.529 ± 0.159
2.821AsnIle: 2.821 ± 0.456
2.527AsnLys: 2.527 ± 0.328
3.467AsnLeu: 3.467 ± 0.53
0.764AsnMet: 0.764 ± 0.231
2.409AsnAsn: 2.409 ± 0.357
2.938AsnPro: 2.938 ± 0.651
1.587AsnGln: 1.587 ± 0.279
2.468AsnArg: 2.468 ± 0.359
3.173AsnSer: 3.173 ± 0.584
3.526AsnThr: 3.526 ± 0.452
2.762AsnVal: 2.762 ± 0.464
0.529AsnTrp: 0.529 ± 0.141
1.528AsnTyr: 1.528 ± 0.385
0.0AsnXaa: 0.0 ± 0.0
Pro
2.821ProAla: 2.821 ± 0.36
0.47ProCys: 0.47 ± 0.184
3.114ProAsp: 3.114 ± 0.582
3.584ProGlu: 3.584 ± 0.407
1.587ProPhe: 1.587 ± 0.334
3.467ProGly: 3.467 ± 0.555
1.234ProHis: 1.234 ± 0.371
3.173ProIle: 3.173 ± 0.545
2.468ProLys: 2.468 ± 0.433
3.056ProLeu: 3.056 ± 0.43
1.293ProMet: 1.293 ± 0.268
2.527ProAsn: 2.527 ± 0.465
2.115ProPro: 2.115 ± 0.461
1.469ProGln: 1.469 ± 0.383
1.822ProArg: 1.822 ± 0.345
2.997ProSer: 2.997 ± 0.444
2.762ProThr: 2.762 ± 0.37
3.291ProVal: 3.291 ± 0.479
0.881ProTrp: 0.881 ± 0.239
1.645ProTyr: 1.645 ± 0.279
0.0ProXaa: 0.0 ± 0.0
Gln
2.703GlnAla: 2.703 ± 0.543
0.176GlnCys: 0.176 ± 0.079
1.469GlnAsp: 1.469 ± 0.313
1.469GlnGlu: 1.469 ± 0.238
1.469GlnPhe: 1.469 ± 0.298
1.763GlnGly: 1.763 ± 0.278
0.529GlnHis: 0.529 ± 0.171
2.527GlnIle: 2.527 ± 0.404
1.645GlnLys: 1.645 ± 0.446
3.408GlnLeu: 3.408 ± 0.422
0.823GlnMet: 0.823 ± 0.207
1.528GlnAsn: 1.528 ± 0.394
1.293GlnPro: 1.293 ± 0.396
1.469GlnGln: 1.469 ± 0.53
1.528GlnArg: 1.528 ± 0.349
2.409GlnSer: 2.409 ± 0.362
1.528GlnThr: 1.528 ± 0.3
2.644GlnVal: 2.644 ± 0.413
0.529GlnTrp: 0.529 ± 0.172
0.823GlnTyr: 0.823 ± 0.203
0.0GlnXaa: 0.0 ± 0.0
Arg
3.819ArgAla: 3.819 ± 0.449
0.47ArgCys: 0.47 ± 0.159
3.467ArgAsp: 3.467 ± 0.367
3.996ArgGlu: 3.996 ± 0.541
2.821ArgPhe: 2.821 ± 0.386
4.701ArgGly: 4.701 ± 0.523
0.94ArgHis: 0.94 ± 0.2
3.878ArgIle: 3.878 ± 0.478
4.642ArgLys: 4.642 ± 0.582
4.231ArgLeu: 4.231 ± 0.565
1.41ArgMet: 1.41 ± 0.27
2.292ArgAsn: 2.292 ± 0.335
3.232ArgPro: 3.232 ± 0.464
2.115ArgGln: 2.115 ± 0.426
3.526ArgArg: 3.526 ± 0.477
2.762ArgSer: 2.762 ± 0.551
2.762ArgThr: 2.762 ± 0.351
3.937ArgVal: 3.937 ± 0.473
0.529ArgTrp: 0.529 ± 0.154
1.645ArgTyr: 1.645 ± 0.334
0.0ArgXaa: 0.0 ± 0.0
Ser
5.994SerAla: 5.994 ± 0.937
0.588SerCys: 0.588 ± 0.235
4.818SerAsp: 4.818 ± 0.493
4.76SerGlu: 4.76 ± 0.515
3.349SerPhe: 3.349 ± 0.385
4.701SerGly: 4.701 ± 0.532
0.881SerHis: 0.881 ± 0.223
3.173SerIle: 3.173 ± 0.391
3.291SerLys: 3.291 ± 0.557
5.524SerLeu: 5.524 ± 0.677
2.468SerMet: 2.468 ± 0.314
3.408SerAsn: 3.408 ± 0.359
3.291SerPro: 3.291 ± 0.598
1.704SerGln: 1.704 ± 0.267
3.232SerArg: 3.232 ± 0.47
4.231SerSer: 4.231 ± 0.705
3.232SerThr: 3.232 ± 0.474
4.936SerVal: 4.936 ± 0.654
0.823SerTrp: 0.823 ± 0.222
2.468SerTyr: 2.468 ± 0.497
0.0SerXaa: 0.0 ± 0.0
Thr
4.525ThrAla: 4.525 ± 0.588
0.294ThrCys: 0.294 ± 0.128
3.937ThrAsp: 3.937 ± 0.524
3.467ThrGlu: 3.467 ± 0.443
2.35ThrPhe: 2.35 ± 0.421
4.348ThrGly: 4.348 ± 0.48
1.175ThrHis: 1.175 ± 0.249
3.643ThrIle: 3.643 ± 0.411
3.408ThrLys: 3.408 ± 0.53
4.642ThrLeu: 4.642 ± 0.483
1.587ThrMet: 1.587 ± 0.337
2.585ThrAsn: 2.585 ± 0.439
2.527ThrPro: 2.527 ± 0.384
1.822ThrGln: 1.822 ± 0.337
3.526ThrArg: 3.526 ± 0.465
3.643ThrSer: 3.643 ± 0.519
2.997ThrThr: 2.997 ± 0.438
6.111ThrVal: 6.111 ± 0.704
1.116ThrTrp: 1.116 ± 0.26
2.233ThrTyr: 2.233 ± 0.4
0.0ThrXaa: 0.0 ± 0.0
Val
5.23ValAla: 5.23 ± 0.453
0.705ValCys: 0.705 ± 0.192
5.053ValAsp: 5.053 ± 0.432
4.818ValGlu: 4.818 ± 0.558
3.467ValPhe: 3.467 ± 0.492
5.641ValGly: 5.641 ± 0.48
0.646ValHis: 0.646 ± 0.15
4.76ValIle: 4.76 ± 0.555
3.467ValLys: 3.467 ± 0.364
3.878ValLeu: 3.878 ± 0.419
1.528ValMet: 1.528 ± 0.255
3.702ValAsn: 3.702 ± 0.474
3.114ValPro: 3.114 ± 0.397
1.939ValGln: 1.939 ± 0.253
4.466ValArg: 4.466 ± 0.544
5.876ValSer: 5.876 ± 0.594
4.231ValThr: 4.231 ± 0.646
4.76ValVal: 4.76 ± 0.657
1.234ValTrp: 1.234 ± 0.337
2.821ValTyr: 2.821 ± 0.427
0.0ValXaa: 0.0 ± 0.0
Trp
0.881TrpAla: 0.881 ± 0.191
0.118TrpCys: 0.118 ± 0.081
1.469TrpAsp: 1.469 ± 0.259
1.175TrpGlu: 1.175 ± 0.218
0.764TrpPhe: 0.764 ± 0.238
0.999TrpGly: 0.999 ± 0.266
0.059TrpHis: 0.059 ± 0.053
0.646TrpIle: 0.646 ± 0.166
0.529TrpLys: 0.529 ± 0.197
1.352TrpLeu: 1.352 ± 0.296
0.118TrpMet: 0.118 ± 0.087
0.588TrpAsn: 0.588 ± 0.18
0.588TrpPro: 0.588 ± 0.188
0.705TrpGln: 0.705 ± 0.211
0.999TrpArg: 0.999 ± 0.299
0.823TrpSer: 0.823 ± 0.247
0.823TrpThr: 0.823 ± 0.213
0.881TrpVal: 0.881 ± 0.259
0.294TrpTrp: 0.294 ± 0.133
0.529TrpTyr: 0.529 ± 0.158
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.35TyrAla: 2.35 ± 0.416
0.353TyrCys: 0.353 ± 0.142
2.468TyrAsp: 2.468 ± 0.371
1.763TyrGlu: 1.763 ± 0.341
1.528TyrPhe: 1.528 ± 0.238
2.468TyrGly: 2.468 ± 0.373
0.823TyrHis: 0.823 ± 0.159
1.763TyrIle: 1.763 ± 0.28
1.469TyrLys: 1.469 ± 0.311
2.703TyrLeu: 2.703 ± 0.296
0.823TyrMet: 0.823 ± 0.177
1.587TyrAsn: 1.587 ± 0.364
2.35TyrPro: 2.35 ± 0.512
1.528TyrGln: 1.528 ± 0.382
1.763TyrArg: 1.763 ± 0.295
2.292TyrSer: 2.292 ± 0.369
1.587TyrThr: 1.587 ± 0.283
2.174TyrVal: 2.174 ± 0.449
0.353TyrTrp: 0.353 ± 0.137
1.528TyrTyr: 1.528 ± 0.31
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (17019 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski