Sphaeramia orbicularis (orbiculate cardinalfish)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei; Eurypterygia;

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57747 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A672ZJJ9|A0A672ZJJ9_9TELE Uncharacterized protein OS=Sphaeramia orbicularis OX=375764 GN=ckap5 PE=4 SV=1
SS1 pKa = 6.66WLHH4 pKa = 5.0LTEE7 pKa = 6.12LLFFWFLSLTGSVVCAVKK25 pKa = 9.65ATDD28 pKa = 4.31EE29 pKa = 4.72DD30 pKa = 4.05IEE32 pKa = 4.49MNAEE36 pKa = 3.94LHH38 pKa = 5.85YY39 pKa = 11.05SLYY42 pKa = 10.81GPSADD47 pKa = 4.56LFSIDD52 pKa = 4.59PYY54 pKa = 10.74SGTVFTSAALRR65 pKa = 11.84RR66 pKa = 11.84TEE68 pKa = 4.79DD69 pKa = 3.17VILNVHH75 pKa = 5.9VEE77 pKa = 4.22DD78 pKa = 4.65AGEE81 pKa = 3.91NPKK84 pKa = 10.52YY85 pKa = 10.96DD86 pKa = 3.47FTTVSIRR93 pKa = 11.84FQNVSEE99 pKa = 4.27FPEE102 pKa = 3.97IRR104 pKa = 11.84VDD106 pKa = 3.33PCEE109 pKa = 4.04VFEE112 pKa = 4.56NSPPSWVCDD121 pKa = 3.53VLAIDD126 pKa = 4.9ADD128 pKa = 3.81SGIYY132 pKa = 9.18GTVQYY137 pKa = 10.97NITEE141 pKa = 4.2GNMDD145 pKa = 4.62YY146 pKa = 11.0IFIIDD151 pKa = 4.36PEE153 pKa = 4.83NGLLSTTASLDD164 pKa = 3.43RR165 pKa = 11.84EE166 pKa = 4.44NIPEE170 pKa = 3.95FNLTVEE176 pKa = 4.29AAEE179 pKa = 5.03LDD181 pKa = 4.04NPLHH185 pKa = 7.04KK186 pKa = 10.71DD187 pKa = 2.89RR188 pKa = 11.84ATVIVSVLDD197 pKa = 4.02RR198 pKa = 11.84NDD200 pKa = 3.21NAPRR204 pKa = 11.84FAQIFISEE212 pKa = 4.38VPEE215 pKa = 4.25DD216 pKa = 3.87APIGQTVIQVTSTDD230 pKa = 4.19DD231 pKa = 3.41DD232 pKa = 3.66TDD234 pKa = 3.72AYY236 pKa = 10.89AGINYY241 pKa = 9.22SIRR244 pKa = 11.84VIAIPYY250 pKa = 8.74

Molecular weight: 27.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A673AX36|A0A673AX36_9TELE Amidophosphoribosyltransferase OS=Sphaeramia orbicularis OX=375764 GN=ppat PE=3 SV=1
VV1 pKa = 6.64SLRR4 pKa = 11.84PTVSSRR10 pKa = 11.84PTVSSRR16 pKa = 11.84PTVSLRR22 pKa = 11.84PTVSLRR28 pKa = 11.84PTVSLRR34 pKa = 11.84PTVSSRR40 pKa = 11.84PTVSLRR46 pKa = 11.84PTVSSRR52 pKa = 11.84PTVSSRR58 pKa = 11.84PTVSLRR64 pKa = 11.84PTVSSRR70 pKa = 11.84PTVSLRR76 pKa = 11.84PTVSSRR82 pKa = 11.84PTVSSRR88 pKa = 11.84PTVSLRR94 pKa = 11.84PTVSSRR100 pKa = 11.84PTVSLRR106 pKa = 11.84PTVSSRR112 pKa = 11.84PTVSSRR118 pKa = 11.84PTVSLRR124 pKa = 11.84PTVSSRR130 pKa = 11.84PTVSLRR136 pKa = 11.84PTVSSRR142 pKa = 11.84PTVSLRR148 pKa = 11.84PTVSLRR154 pKa = 11.84PTVSSRR160 pKa = 11.84PTVSSRR166 pKa = 11.84PTVSSRR172 pKa = 11.84PTVSSRR178 pKa = 11.84PTVSLRR184 pKa = 11.84PTVSSRR190 pKa = 11.84PTVSLRR196 pKa = 11.84SQSHH200 pKa = 3.91WTHH203 pKa = 6.28FLGGVPDD210 pKa = 3.6EE211 pKa = 4.79RR212 pKa = 11.84GG213 pKa = 3.03

Molecular weight: 22.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

24323

33424

57747

36437828

17

7265

631.0

70.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.323 ± 0.008

2.34 ± 0.009

5.323 ± 0.007

6.698 ± 0.012

3.894 ± 0.007

6.21 ± 0.011

2.68 ± 0.005

4.703 ± 0.007

5.696 ± 0.011

9.747 ± 0.013

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.437 ± 0.004

3.963 ± 0.006

5.347 ± 0.011

4.656 ± 0.01

5.486 ± 0.007

8.115 ± 0.011

5.682 ± 0.012

6.547 ± 0.008

1.205 ± 0.003

2.947 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski