Amino acid dipepetide frequency for Escherichia phage vB_EcoP_24B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.83AlaAla: 7.83 ± 0.861
1.587AlaCys: 1.587 ± 0.424
4.867AlaAsp: 4.867 ± 0.55
7.512AlaGlu: 7.512 ± 0.939
3.65AlaPhe: 3.65 ± 0.462
7.512AlaGly: 7.512 ± 1.155
1.746AlaHis: 1.746 ± 0.316
3.756AlaIle: 3.756 ± 0.488
4.444AlaLys: 4.444 ± 0.505
6.719AlaLeu: 6.719 ± 0.645
3.068AlaMet: 3.068 ± 0.451
3.121AlaAsn: 3.121 ± 0.4
3.016AlaPro: 3.016 ± 0.439
5.185AlaGln: 5.185 ± 0.758
5.661AlaArg: 5.661 ± 0.609
5.502AlaSer: 5.502 ± 0.474
5.185AlaThr: 5.185 ± 0.817
6.19AlaVal: 6.19 ± 0.745
1.64AlaTrp: 1.64 ± 0.286
1.905AlaTyr: 1.905 ± 0.328
0.0AlaXaa: 0.0 ± 0.0
Cys
1.323CysAla: 1.323 ± 0.328
0.529CysCys: 0.529 ± 0.168
0.635CysAsp: 0.635 ± 0.203
1.111CysGlu: 1.111 ± 0.309
0.688CysPhe: 0.688 ± 0.257
1.005CysGly: 1.005 ± 0.276
0.476CysHis: 0.476 ± 0.21
1.005CysIle: 1.005 ± 0.317
0.529CysLys: 0.529 ± 0.19
0.952CysLeu: 0.952 ± 0.279
0.159CysMet: 0.159 ± 0.071
0.212CysAsn: 0.212 ± 0.112
0.794CysPro: 0.794 ± 0.301
0.159CysGln: 0.159 ± 0.089
1.957CysArg: 1.957 ± 0.526
1.217CysSer: 1.217 ± 0.306
0.741CysThr: 0.741 ± 0.202
0.952CysVal: 0.952 ± 0.298
0.265CysTrp: 0.265 ± 0.135
0.37CysTyr: 0.37 ± 0.163
0.0CysXaa: 0.0 ± 0.0
Asp
5.767AspAla: 5.767 ± 0.642
0.688AspCys: 0.688 ± 0.209
3.809AspAsp: 3.809 ± 0.44
4.074AspGlu: 4.074 ± 0.424
1.481AspPhe: 1.481 ± 0.25
4.867AspGly: 4.867 ± 0.53
0.952AspHis: 0.952 ± 0.342
3.439AspIle: 3.439 ± 0.365
3.809AspLys: 3.809 ± 0.371
3.703AspLeu: 3.703 ± 0.411
1.534AspMet: 1.534 ± 0.252
2.857AspAsn: 2.857 ± 0.467
2.222AspPro: 2.222 ± 0.425
1.481AspGln: 1.481 ± 0.337
3.492AspArg: 3.492 ± 0.353
2.751AspSer: 2.751 ± 0.322
2.539AspThr: 2.539 ± 0.433
4.021AspVal: 4.021 ± 0.385
1.005AspTrp: 1.005 ± 0.294
1.323AspTyr: 1.323 ± 0.282
0.0AspXaa: 0.0 ± 0.0
Glu
6.401GluAla: 6.401 ± 0.61
1.005GluCys: 1.005 ± 0.304
2.01GluAsp: 2.01 ± 0.274
3.598GluGlu: 3.598 ± 0.399
2.434GluPhe: 2.434 ± 0.395
3.756GluGly: 3.756 ± 0.529
1.323GluHis: 1.323 ± 0.237
3.915GluIle: 3.915 ± 0.485
4.497GluLys: 4.497 ± 0.586
5.449GluLeu: 5.449 ± 0.593
2.116GluMet: 2.116 ± 0.358
2.592GluAsn: 2.592 ± 0.436
1.905GluPro: 1.905 ± 0.371
3.968GluGln: 3.968 ± 0.55
5.238GluArg: 5.238 ± 0.592
3.809GluSer: 3.809 ± 0.406
3.809GluThr: 3.809 ± 0.635
4.021GluVal: 4.021 ± 0.585
1.111GluTrp: 1.111 ± 0.183
2.222GluTyr: 2.222 ± 0.306
0.0GluXaa: 0.0 ± 0.0
Phe
2.539PheAla: 2.539 ± 0.425
0.423PheCys: 0.423 ± 0.171
1.852PheAsp: 1.852 ± 0.323
1.799PheGlu: 1.799 ± 0.405
1.64PhePhe: 1.64 ± 0.422
2.381PheGly: 2.381 ± 0.223
1.111PheHis: 1.111 ± 0.242
2.169PheIle: 2.169 ± 0.334
1.693PheLys: 1.693 ± 0.29
2.328PheLeu: 2.328 ± 0.299
1.005PheMet: 1.005 ± 0.269
1.164PheAsn: 1.164 ± 0.28
1.587PhePro: 1.587 ± 0.316
1.058PheGln: 1.058 ± 0.217
2.698PheArg: 2.698 ± 0.444
3.386PheSer: 3.386 ± 0.47
2.487PheThr: 2.487 ± 0.392
2.116PheVal: 2.116 ± 0.304
0.741PheTrp: 0.741 ± 0.202
1.164PheTyr: 1.164 ± 0.217
0.0PheXaa: 0.0 ± 0.0
Gly
6.084GlyAla: 6.084 ± 0.893
1.217GlyCys: 1.217 ± 0.332
4.656GlyAsp: 4.656 ± 0.852
5.978GlyGlu: 5.978 ± 1.447
2.857GlyPhe: 2.857 ± 0.4
5.238GlyGly: 5.238 ± 0.638
1.376GlyHis: 1.376 ± 0.307
4.391GlyIle: 4.391 ± 0.669
4.55GlyLys: 4.55 ± 0.737
5.132GlyLeu: 5.132 ± 0.511
2.487GlyMet: 2.487 ± 0.311
3.068GlyAsn: 3.068 ± 0.371
3.809GlyPro: 3.809 ± 2.356
2.539GlyGln: 2.539 ± 0.356
4.074GlyArg: 4.074 ± 0.461
3.65GlySer: 3.65 ± 0.461
3.545GlyThr: 3.545 ± 0.475
5.502GlyVal: 5.502 ± 0.534
1.217GlyTrp: 1.217 ± 0.254
2.487GlyTyr: 2.487 ± 0.366
0.0GlyXaa: 0.0 ± 0.0
His
1.428HisAla: 1.428 ± 0.245
0.476HisCys: 0.476 ± 0.154
1.27HisAsp: 1.27 ± 0.298
0.846HisGlu: 0.846 ± 0.245
1.111HisPhe: 1.111 ± 0.273
2.063HisGly: 2.063 ± 0.451
0.476HisHis: 0.476 ± 0.15
1.111HisIle: 1.111 ± 0.297
0.952HisLys: 0.952 ± 0.244
1.693HisLeu: 1.693 ± 0.35
0.317HisMet: 0.317 ± 0.113
0.952HisAsn: 0.952 ± 0.176
1.164HisPro: 1.164 ± 0.314
0.952HisGln: 0.952 ± 0.26
1.323HisArg: 1.323 ± 0.321
0.952HisSer: 0.952 ± 0.199
1.058HisThr: 1.058 ± 0.342
1.058HisVal: 1.058 ± 0.206
0.317HisTrp: 0.317 ± 0.156
0.688HisTyr: 0.688 ± 0.173
0.0HisXaa: 0.0 ± 0.0
Ile
4.127IleAla: 4.127 ± 0.496
1.005IleCys: 1.005 ± 0.255
3.439IleAsp: 3.439 ± 0.416
2.857IleGlu: 2.857 ± 0.371
1.693IlePhe: 1.693 ± 0.303
2.963IleGly: 2.963 ± 0.601
1.058IleHis: 1.058 ± 0.257
2.222IleIle: 2.222 ± 0.288
2.857IleLys: 2.857 ± 0.398
3.174IleLeu: 3.174 ± 0.533
1.27IleMet: 1.27 ± 0.232
2.857IleAsn: 2.857 ± 0.389
2.645IlePro: 2.645 ± 0.387
2.063IleGln: 2.063 ± 0.252
4.497IleArg: 4.497 ± 0.477
4.285IleSer: 4.285 ± 0.527
3.598IleThr: 3.598 ± 0.548
2.169IleVal: 2.169 ± 0.328
0.635IleTrp: 0.635 ± 0.227
1.693IleTyr: 1.693 ± 0.324
0.0IleXaa: 0.0 ± 0.0
Lys
5.819LysAla: 5.819 ± 0.585
0.635LysCys: 0.635 ± 0.204
2.804LysAsp: 2.804 ± 0.376
3.28LysGlu: 3.28 ± 0.385
1.27LysPhe: 1.27 ± 0.281
5.079LysGly: 5.079 ± 1.114
1.058LysHis: 1.058 ± 0.248
3.492LysIle: 3.492 ± 0.382
4.127LysLys: 4.127 ± 0.594
4.761LysLeu: 4.761 ± 0.54
1.957LysMet: 1.957 ± 0.303
3.492LysAsn: 3.492 ± 0.446
2.804LysPro: 2.804 ± 0.411
3.068LysGln: 3.068 ± 0.464
3.068LysArg: 3.068 ± 0.423
2.698LysSer: 2.698 ± 0.344
3.333LysThr: 3.333 ± 0.393
2.963LysVal: 2.963 ± 0.423
0.794LysTrp: 0.794 ± 0.229
1.64LysTyr: 1.64 ± 0.285
0.0LysXaa: 0.0 ± 0.0
Leu
7.989LeuAla: 7.989 ± 0.776
1.746LeuCys: 1.746 ± 0.417
3.862LeuAsp: 3.862 ± 0.444
3.756LeuGlu: 3.756 ± 0.535
2.804LeuPhe: 2.804 ± 0.365
4.074LeuGly: 4.074 ± 0.491
1.481LeuHis: 1.481 ± 0.314
3.333LeuIle: 3.333 ± 0.484
5.026LeuLys: 5.026 ± 0.43
6.243LeuLeu: 6.243 ± 0.634
2.275LeuMet: 2.275 ± 0.436
4.338LeuAsn: 4.338 ± 0.522
4.232LeuPro: 4.232 ± 0.433
3.28LeuGln: 3.28 ± 0.606
5.238LeuArg: 5.238 ± 0.638
5.502LeuSer: 5.502 ± 0.557
4.867LeuThr: 4.867 ± 0.39
4.444LeuVal: 4.444 ± 0.444
1.111LeuTrp: 1.111 ± 0.339
2.381LeuTyr: 2.381 ± 0.415
0.0LeuXaa: 0.0 ± 0.0
Met
3.227MetAla: 3.227 ± 0.341
0.212MetCys: 0.212 ± 0.105
1.428MetAsp: 1.428 ± 0.271
1.27MetGlu: 1.27 ± 0.265
0.688MetPhe: 0.688 ± 0.156
1.27MetGly: 1.27 ± 0.275
0.476MetHis: 0.476 ± 0.141
0.899MetIle: 0.899 ± 0.219
2.063MetLys: 2.063 ± 0.288
2.328MetLeu: 2.328 ± 0.305
0.688MetMet: 0.688 ± 0.176
1.905MetAsn: 1.905 ± 0.342
1.905MetPro: 1.905 ± 0.291
1.27MetGln: 1.27 ± 0.218
1.852MetArg: 1.852 ± 0.28
2.169MetSer: 2.169 ± 0.324
2.116MetThr: 2.116 ± 0.284
1.111MetVal: 1.111 ± 0.253
0.265MetTrp: 0.265 ± 0.106
0.635MetTyr: 0.635 ± 0.19
0.0MetXaa: 0.0 ± 0.0
Asn
4.656AsnAla: 4.656 ± 0.607
0.317AsnCys: 0.317 ± 0.13
2.063AsnAsp: 2.063 ± 0.319
3.28AsnGlu: 3.28 ± 0.362
1.376AsnPhe: 1.376 ± 0.258
4.127AsnGly: 4.127 ± 0.534
1.111AsnHis: 1.111 ± 0.229
2.804AsnIle: 2.804 ± 0.46
2.539AsnLys: 2.539 ± 0.385
3.333AsnLeu: 3.333 ± 0.376
1.376AsnMet: 1.376 ± 0.263
1.746AsnAsn: 1.746 ± 0.32
2.063AsnPro: 2.063 ± 0.279
2.01AsnGln: 2.01 ± 0.415
2.275AsnArg: 2.275 ± 0.383
2.539AsnSer: 2.539 ± 0.329
1.905AsnThr: 1.905 ± 0.262
2.063AsnVal: 2.063 ± 0.314
0.37AsnTrp: 0.37 ± 0.125
1.111AsnTyr: 1.111 ± 0.296
0.0AsnXaa: 0.0 ± 0.0
Pro
3.492ProAla: 3.492 ± 0.614
0.635ProCys: 0.635 ± 0.166
4.285ProAsp: 4.285 ± 0.405
4.92ProGlu: 4.92 ± 0.754
1.376ProPhe: 1.376 ± 0.315
2.963ProGly: 2.963 ± 0.543
0.846ProHis: 0.846 ± 0.21
1.27ProIle: 1.27 ± 0.3
2.698ProLys: 2.698 ± 0.765
3.016ProLeu: 3.016 ± 0.416
0.688ProMet: 0.688 ± 0.176
1.111ProAsn: 1.111 ± 0.239
1.587ProPro: 1.587 ± 0.317
2.328ProGln: 2.328 ± 0.579
2.328ProArg: 2.328 ± 0.384
2.963ProSer: 2.963 ± 0.431
1.746ProThr: 1.746 ± 0.295
4.92ProVal: 4.92 ± 0.627
0.688ProTrp: 0.688 ± 0.188
1.905ProTyr: 1.905 ± 0.318
0.0ProXaa: 0.0 ± 0.0
Gln
4.497GlnAla: 4.497 ± 0.668
0.741GlnCys: 0.741 ± 0.197
2.116GlnAsp: 2.116 ± 0.364
3.068GlnGlu: 3.068 ± 0.486
1.693GlnPhe: 1.693 ± 0.334
3.227GlnGly: 3.227 ± 0.787
0.952GlnHis: 0.952 ± 0.219
1.799GlnIle: 1.799 ± 0.332
2.963GlnLys: 2.963 ± 0.514
3.598GlnLeu: 3.598 ± 0.485
1.058GlnMet: 1.058 ± 0.313
1.534GlnAsn: 1.534 ± 0.323
2.063GlnPro: 2.063 ± 0.501
3.333GlnGln: 3.333 ± 0.877
3.227GlnArg: 3.227 ± 0.478
2.487GlnSer: 2.487 ± 0.315
2.169GlnThr: 2.169 ± 0.475
2.487GlnVal: 2.487 ± 0.428
0.846GlnTrp: 0.846 ± 0.198
1.481GlnTyr: 1.481 ± 0.247
0.0GlnXaa: 0.0 ± 0.0
Arg
4.55ArgAla: 4.55 ± 0.53
0.476ArgCys: 0.476 ± 0.16
3.492ArgAsp: 3.492 ± 0.568
4.814ArgGlu: 4.814 ± 0.681
2.381ArgPhe: 2.381 ± 0.32
5.29ArgGly: 5.29 ± 0.858
1.905ArgHis: 1.905 ± 0.346
3.333ArgIle: 3.333 ± 0.424
4.285ArgLys: 4.285 ± 0.48
5.925ArgLeu: 5.925 ± 0.577
2.116ArgMet: 2.116 ± 0.28
3.333ArgAsn: 3.333 ± 0.394
2.434ArgPro: 2.434 ± 0.494
3.174ArgGln: 3.174 ± 0.428
5.767ArgArg: 5.767 ± 0.717
4.232ArgSer: 4.232 ± 0.473
3.862ArgThr: 3.862 ± 0.531
4.127ArgVal: 4.127 ± 0.495
1.164ArgTrp: 1.164 ± 0.191
2.592ArgTyr: 2.592 ± 0.409
0.0ArgXaa: 0.0 ± 0.0
Ser
5.767SerAla: 5.767 ± 0.58
0.846SerCys: 0.846 ± 0.325
3.915SerAsp: 3.915 ± 0.477
4.127SerGlu: 4.127 ± 0.449
2.01SerPhe: 2.01 ± 0.352
6.296SerGly: 6.296 ± 0.49
0.952SerHis: 0.952 ± 0.213
2.751SerIle: 2.751 ± 0.337
2.751SerLys: 2.751 ± 0.437
6.084SerLeu: 6.084 ± 0.642
1.481SerMet: 1.481 ± 0.275
2.275SerAsn: 2.275 ± 0.278
3.65SerPro: 3.65 ± 0.409
2.804SerGln: 2.804 ± 0.428
4.55SerArg: 4.55 ± 0.613
3.28SerSer: 3.28 ± 0.62
3.174SerThr: 3.174 ± 0.409
3.915SerVal: 3.915 ± 0.422
0.741SerTrp: 0.741 ± 0.199
1.534SerTyr: 1.534 ± 0.276
0.0SerXaa: 0.0 ± 0.0
Thr
5.29ThrAla: 5.29 ± 0.448
0.794ThrCys: 0.794 ± 0.281
3.068ThrAsp: 3.068 ± 0.452
3.809ThrGlu: 3.809 ± 0.367
1.799ThrPhe: 1.799 ± 0.397
5.608ThrGly: 5.608 ± 0.811
1.164ThrHis: 1.164 ± 0.272
3.068ThrIle: 3.068 ± 0.43
2.91ThrLys: 2.91 ± 0.34
4.603ThrLeu: 4.603 ± 0.434
0.952ThrMet: 0.952 ± 0.17
1.746ThrAsn: 1.746 ± 0.278
3.174ThrPro: 3.174 ± 0.395
1.746ThrGln: 1.746 ± 0.335
2.963ThrArg: 2.963 ± 0.465
4.021ThrSer: 4.021 ± 0.514
2.963ThrThr: 2.963 ± 0.416
3.915ThrVal: 3.915 ± 0.514
0.741ThrTrp: 0.741 ± 0.15
1.27ThrTyr: 1.27 ± 0.289
0.0ThrXaa: 0.0 ± 0.0
Val
5.819ValAla: 5.819 ± 0.579
1.111ValCys: 1.111 ± 0.25
3.968ValAsp: 3.968 ± 0.487
3.386ValGlu: 3.386 ± 0.333
2.328ValPhe: 2.328 ± 0.312
3.227ValGly: 3.227 ± 0.46
0.635ValHis: 0.635 ± 0.183
3.915ValIle: 3.915 ± 0.494
3.333ValLys: 3.333 ± 0.381
5.608ValLeu: 5.608 ± 0.654
1.587ValMet: 1.587 ± 0.264
2.857ValAsn: 2.857 ± 0.364
2.592ValPro: 2.592 ± 0.296
2.381ValGln: 2.381 ± 0.518
4.814ValArg: 4.814 ± 0.838
4.603ValSer: 4.603 ± 0.536
4.338ValThr: 4.338 ± 0.559
4.074ValVal: 4.074 ± 0.406
0.688ValTrp: 0.688 ± 0.152
1.64ValTyr: 1.64 ± 0.253
0.0ValXaa: 0.0 ± 0.0
Trp
0.846TrpAla: 0.846 ± 0.266
0.265TrpCys: 0.265 ± 0.119
0.688TrpAsp: 0.688 ± 0.195
0.635TrpGlu: 0.635 ± 0.171
0.688TrpPhe: 0.688 ± 0.156
0.582TrpGly: 0.582 ± 0.159
0.37TrpHis: 0.37 ± 0.148
0.794TrpIle: 0.794 ± 0.223
1.005TrpLys: 1.005 ± 0.233
1.693TrpLeu: 1.693 ± 0.354
0.635TrpMet: 0.635 ± 0.141
0.582TrpAsn: 0.582 ± 0.175
0.688TrpPro: 0.688 ± 0.218
1.27TrpGln: 1.27 ± 0.213
1.376TrpArg: 1.376 ± 0.279
0.899TrpSer: 0.899 ± 0.216
0.582TrpThr: 0.582 ± 0.195
0.952TrpVal: 0.952 ± 0.245
0.317TrpTrp: 0.317 ± 0.134
0.37TrpTyr: 0.37 ± 0.139
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.645TyrAla: 2.645 ± 0.405
0.37TyrCys: 0.37 ± 0.181
1.587TyrAsp: 1.587 ± 0.28
1.058TyrGlu: 1.058 ± 0.249
1.376TyrPhe: 1.376 ± 0.272
2.328TyrGly: 2.328 ± 0.386
0.741TyrHis: 0.741 ± 0.189
1.64TyrIle: 1.64 ± 0.307
1.111TyrLys: 1.111 ± 0.222
1.64TyrLeu: 1.64 ± 0.273
0.952TyrMet: 0.952 ± 0.235
1.323TyrAsn: 1.323 ± 0.239
1.428TyrPro: 1.428 ± 0.229
1.323TyrGln: 1.323 ± 0.207
2.857TyrArg: 2.857 ± 0.472
1.957TyrSer: 1.957 ± 0.297
1.587TyrThr: 1.587 ± 0.378
1.905TyrVal: 1.905 ± 0.334
0.582TyrTrp: 0.582 ± 0.179
0.952TyrTyr: 0.952 ± 0.199
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 88 proteins (18903 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski