Amino acid dipepetide frequency for Comamonas sp. BIGb0124

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.895AlaAla: 17.895 ± 0.155
1.308AlaCys: 1.308 ± 0.032
6.779AlaAsp: 6.779 ± 0.067
6.455AlaGlu: 6.455 ± 0.076
3.938AlaPhe: 3.938 ± 0.057
11.242AlaGly: 11.242 ± 0.09
2.749AlaHis: 2.749 ± 0.046
5.304AlaIle: 5.304 ± 0.072
3.103AlaLys: 3.103 ± 0.062
15.227AlaLeu: 15.227 ± 0.133
3.435AlaMet: 3.435 ± 0.053
2.563AlaAsn: 2.563 ± 0.052
6.17AlaPro: 6.17 ± 0.089
6.175AlaGln: 6.175 ± 0.067
9.247AlaArg: 9.247 ± 0.093
7.214AlaSer: 7.214 ± 0.07
6.138AlaThr: 6.138 ± 0.067
9.571AlaVal: 9.571 ± 0.088
1.975AlaTrp: 1.975 ± 0.045
2.816AlaTyr: 2.816 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
1.047CysAla: 1.047 ± 0.028
0.104CysCys: 0.104 ± 0.008
0.469CysAsp: 0.469 ± 0.017
0.426CysGlu: 0.426 ± 0.019
0.284CysPhe: 0.284 ± 0.013
0.9CysGly: 0.9 ± 0.026
0.236CysHis: 0.236 ± 0.013
0.36CysIle: 0.36 ± 0.015
0.178CysLys: 0.178 ± 0.012
0.793CysLeu: 0.793 ± 0.023
0.189CysMet: 0.189 ± 0.012
0.191CysAsn: 0.191 ± 0.012
0.406CysPro: 0.406 ± 0.019
0.232CysGln: 0.232 ± 0.014
0.574CysArg: 0.574 ± 0.021
0.473CysSer: 0.473 ± 0.018
0.491CysThr: 0.491 ± 0.019
0.66CysVal: 0.66 ± 0.021
0.127CysTrp: 0.127 ± 0.009
0.183CysTyr: 0.183 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
6.883AspAla: 6.883 ± 0.075
0.417AspCys: 0.417 ± 0.015
2.803AspAsp: 2.803 ± 0.051
3.093AspGlu: 3.093 ± 0.049
2.027AspPhe: 2.027 ± 0.042
4.813AspGly: 4.813 ± 0.065
1.144AspHis: 1.144 ± 0.032
2.544AspIle: 2.544 ± 0.048
1.483AspLys: 1.483 ± 0.038
5.498AspLeu: 5.498 ± 0.067
1.258AspMet: 1.258 ± 0.027
1.254AspAsn: 1.254 ± 0.035
2.968AspPro: 2.968 ± 0.045
1.696AspGln: 1.696 ± 0.037
3.694AspArg: 3.694 ± 0.052
2.505AspSer: 2.505 ± 0.089
2.574AspThr: 2.574 ± 0.044
4.194AspVal: 4.194 ± 0.055
0.992AspTrp: 0.992 ± 0.028
1.403AspTyr: 1.403 ± 0.034
0.0AspXaa: 0.0 ± 0.0
Glu
6.361GluAla: 6.361 ± 0.059
0.297GluCys: 0.297 ± 0.016
2.176GluAsp: 2.176 ± 0.047
2.173GluGlu: 2.173 ± 0.051
1.547GluPhe: 1.547 ± 0.036
3.642GluGly: 3.642 ± 0.055
1.265GluHis: 1.265 ± 0.034
2.541GluIle: 2.541 ± 0.045
1.506GluLys: 1.506 ± 0.047
5.542GluLeu: 5.542 ± 0.074
0.987GluMet: 0.987 ± 0.028
1.228GluAsn: 1.228 ± 0.03
2.606GluPro: 2.606 ± 0.045
2.747GluGln: 2.747 ± 0.054
4.265GluArg: 4.265 ± 0.062
2.746GluSer: 2.746 ± 0.044
2.562GluThr: 2.562 ± 0.041
3.549GluVal: 3.549 ± 0.052
0.672GluTrp: 0.672 ± 0.02
0.918GluTyr: 0.918 ± 0.025
0.0GluXaa: 0.0 ± 0.0
Phe
3.839PheAla: 3.839 ± 0.057
0.349PheCys: 0.349 ± 0.016
2.467PheAsp: 2.467 ± 0.043
1.963PheGlu: 1.963 ± 0.04
1.331PhePhe: 1.331 ± 0.04
3.184PheGly: 3.184 ± 0.055
0.663PheHis: 0.663 ± 0.02
1.444PheIle: 1.444 ± 0.034
1.119PheLys: 1.119 ± 0.029
2.891PheLeu: 2.891 ± 0.047
0.838PheMet: 0.838 ± 0.024
1.041PheAsn: 1.041 ± 0.024
1.423PhePro: 1.423 ± 0.03
1.068PheGln: 1.068 ± 0.027
1.724PheArg: 1.724 ± 0.031
2.123PheSer: 2.123 ± 0.042
1.728PheThr: 1.728 ± 0.034
2.764PheVal: 2.764 ± 0.047
0.498PheTrp: 0.498 ± 0.02
0.857PheTyr: 0.857 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
8.864GlyAla: 8.864 ± 0.079
0.808GlyCys: 0.808 ± 0.023
3.937GlyAsp: 3.937 ± 0.057
4.24GlyGlu: 4.24 ± 0.063
3.099GlyPhe: 3.099 ± 0.05
7.083GlyGly: 7.083 ± 0.09
2.125GlyHis: 2.125 ± 0.044
4.033GlyIle: 4.033 ± 0.061
2.784GlyLys: 2.784 ± 0.049
9.97GlyLeu: 9.97 ± 0.117
2.247GlyMet: 2.247 ± 0.036
2.152GlyAsn: 2.152 ± 0.042
3.437GlyPro: 3.437 ± 0.06
3.973GlyGln: 3.973 ± 0.054
5.935GlyArg: 5.935 ± 0.076
5.056GlySer: 5.056 ± 0.064
4.672GlyThr: 4.672 ± 0.069
6.627GlyVal: 6.627 ± 0.07
1.494GlyTrp: 1.494 ± 0.037
2.384GlyTyr: 2.384 ± 0.04
0.0GlyXaa: 0.0 ± 0.0
His
3.219HisAla: 3.219 ± 0.054
0.221HisCys: 0.221 ± 0.012
1.436HisAsp: 1.436 ± 0.036
1.186HisGlu: 1.186 ± 0.029
0.791HisPhe: 0.791 ± 0.022
2.196HisGly: 2.196 ± 0.044
0.58HisHis: 0.58 ± 0.022
0.977HisIle: 0.977 ± 0.027
0.443HisLys: 0.443 ± 0.02
2.195HisLeu: 2.195 ± 0.043
0.462HisMet: 0.462 ± 0.018
0.49HisAsn: 0.49 ± 0.018
1.502HisPro: 1.502 ± 0.035
0.712HisGln: 0.712 ± 0.022
1.573HisArg: 1.573 ± 0.034
1.032HisSer: 1.032 ± 0.025
1.09HisThr: 1.09 ± 0.028
1.696HisVal: 1.696 ± 0.032
0.413HisTrp: 0.413 ± 0.018
0.612HisTyr: 0.612 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
5.86IleAla: 5.86 ± 0.072
0.38IleCys: 0.38 ± 0.017
3.077IleAsp: 3.077 ± 0.044
3.034IleGlu: 3.034 ± 0.048
1.233IlePhe: 1.233 ± 0.035
4.311IleGly: 4.311 ± 0.061
0.844IleHis: 0.844 ± 0.024
1.381IleIle: 1.381 ± 0.038
1.355IleLys: 1.355 ± 0.036
3.483IleLeu: 3.483 ± 0.058
0.783IleMet: 0.783 ± 0.024
1.361IleAsn: 1.361 ± 0.033
2.029IlePro: 2.029 ± 0.04
1.509IleGln: 1.509 ± 0.035
2.716IleArg: 2.716 ± 0.045
2.292IleSer: 2.292 ± 0.046
2.25IleThr: 2.25 ± 0.047
3.641IleVal: 3.641 ± 0.057
0.507IleTrp: 0.507 ± 0.019
1.012IleTyr: 1.012 ± 0.029
0.0IleXaa: 0.0 ± 0.0
Lys
3.526LysAla: 3.526 ± 0.07
0.109LysCys: 0.109 ± 0.009
1.377LysAsp: 1.377 ± 0.032
1.142LysGlu: 1.142 ± 0.03
0.782LysPhe: 0.782 ± 0.026
2.149LysGly: 2.149 ± 0.046
0.524LysHis: 0.524 ± 0.021
1.293LysIle: 1.293 ± 0.036
1.096LysLys: 1.096 ± 0.038
3.008LysLeu: 3.008 ± 0.059
0.604LysMet: 0.604 ± 0.022
0.789LysAsn: 0.789 ± 0.027
1.836LysPro: 1.836 ± 0.039
1.08LysGln: 1.08 ± 0.028
1.851LysArg: 1.851 ± 0.042
1.645LysSer: 1.645 ± 0.038
1.78LysThr: 1.78 ± 0.037
2.123LysVal: 2.123 ± 0.044
0.337LysTrp: 0.337 ± 0.015
0.558LysTyr: 0.558 ± 0.023
0.0LysXaa: 0.0 ± 0.0
Leu
15.719LeuAla: 15.719 ± 0.137
0.956LeuCys: 0.956 ± 0.026
6.3LeuAsp: 6.3 ± 0.073
5.064LeuGlu: 5.064 ± 0.064
3.37LeuPhe: 3.37 ± 0.054
9.053LeuGly: 9.053 ± 0.099
2.405LeuHis: 2.405 ± 0.043
4.49LeuIle: 4.49 ± 0.062
3.09LeuLys: 3.09 ± 0.048
11.43LeuLeu: 11.43 ± 0.138
2.469LeuMet: 2.469 ± 0.054
2.614LeuAsn: 2.614 ± 0.047
6.567LeuPro: 6.567 ± 0.074
4.531LeuGln: 4.531 ± 0.065
7.716LeuArg: 7.716 ± 0.085
6.606LeuSer: 6.606 ± 0.077
5.412LeuThr: 5.412 ± 0.064
8.083LeuVal: 8.083 ± 0.083
1.33LeuTrp: 1.33 ± 0.035
2.135LeuTyr: 2.135 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
3.13MetAla: 3.13 ± 0.047
0.153MetCys: 0.153 ± 0.011
1.043MetAsp: 1.043 ± 0.025
0.82MetGlu: 0.82 ± 0.025
0.634MetPhe: 0.634 ± 0.021
1.855MetGly: 1.855 ± 0.039
0.448MetHis: 0.448 ± 0.017
0.923MetIle: 0.923 ± 0.025
0.795MetLys: 0.795 ± 0.026
2.578MetLeu: 2.578 ± 0.049
0.533MetMet: 0.533 ± 0.019
0.781MetAsn: 0.781 ± 0.023
1.455MetPro: 1.455 ± 0.035
0.996MetGln: 0.996 ± 0.028
1.615MetArg: 1.615 ± 0.034
1.63MetSer: 1.63 ± 0.035
1.613MetThr: 1.613 ± 0.03
1.58MetVal: 1.58 ± 0.033
0.182MetTrp: 0.182 ± 0.012
0.315MetTyr: 0.315 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
2.973AsnAla: 2.973 ± 0.044
0.206AsnCys: 0.206 ± 0.013
1.288AsnAsp: 1.288 ± 0.034
1.066AsnGlu: 1.066 ± 0.031
0.855AsnPhe: 0.855 ± 0.027
2.179AsnGly: 2.179 ± 0.047
0.479AsnHis: 0.479 ± 0.018
1.145AsnIle: 1.145 ± 0.029
0.736AsnLys: 0.736 ± 0.026
2.584AsnLeu: 2.584 ± 0.043
0.504AsnMet: 0.504 ± 0.02
0.7AsnAsn: 0.7 ± 0.022
1.772AsnPro: 1.772 ± 0.036
0.835AsnGln: 0.835 ± 0.024
1.736AsnArg: 1.736 ± 0.037
1.172AsnSer: 1.172 ± 0.039
1.453AsnThr: 1.453 ± 0.034
1.857AsnVal: 1.857 ± 0.042
0.403AsnTrp: 0.403 ± 0.019
0.659AsnTyr: 0.659 ± 0.024
0.0AsnXaa: 0.0 ± 0.0
Pro
7.834ProAla: 7.834 ± 0.116
0.364ProCys: 0.364 ± 0.014
3.322ProAsp: 3.322 ± 0.047
3.148ProGlu: 3.148 ± 0.046
1.749ProPhe: 1.749 ± 0.039
4.936ProGly: 4.936 ± 0.068
1.257ProHis: 1.257 ± 0.027
1.984ProIle: 1.984 ± 0.037
1.256ProLys: 1.256 ± 0.03
5.548ProLeu: 5.548 ± 0.069
1.237ProMet: 1.237 ± 0.034
1.21ProAsn: 1.21 ± 0.036
2.809ProPro: 2.809 ± 0.054
2.309ProGln: 2.309 ± 0.039
3.3ProArg: 3.3 ± 0.051
3.253ProSer: 3.253 ± 0.053
2.654ProThr: 2.654 ± 0.041
4.563ProVal: 4.563 ± 0.059
0.806ProTrp: 0.806 ± 0.026
1.262ProTyr: 1.262 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
6.919GlnAla: 6.919 ± 0.076
0.259GlnCys: 0.259 ± 0.013
1.908GlnAsp: 1.908 ± 0.036
1.621GlnGlu: 1.621 ± 0.039
1.24GlnPhe: 1.24 ± 0.031
3.651GlnGly: 3.651 ± 0.052
0.913GlnHis: 0.913 ± 0.026
1.85GlnIle: 1.85 ± 0.036
1.017GlnLys: 1.017 ± 0.027
4.149GlnLeu: 4.149 ± 0.063
0.836GlnMet: 0.836 ± 0.025
0.889GlnAsn: 0.889 ± 0.029
2.734GlnPro: 2.734 ± 0.044
2.052GlnGln: 2.052 ± 0.043
3.468GlnArg: 3.468 ± 0.056
2.245GlnSer: 2.245 ± 0.04
2.133GlnThr: 2.133 ± 0.039
3.114GlnVal: 3.114 ± 0.057
0.681GlnTrp: 0.681 ± 0.02
0.776GlnTyr: 0.776 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
7.824ArgAla: 7.824 ± 0.081
0.554ArgCys: 0.554 ± 0.018
3.519ArgAsp: 3.519 ± 0.051
3.914ArgGlu: 3.914 ± 0.06
2.552ArgPhe: 2.552 ± 0.046
4.628ArgGly: 4.628 ± 0.055
2.115ArgHis: 2.115 ± 0.042
3.555ArgIle: 3.555 ± 0.049
1.954ArgLys: 1.954 ± 0.044
8.467ArgLeu: 8.467 ± 0.088
1.854ArgMet: 1.854 ± 0.037
1.823ArgAsn: 1.823 ± 0.042
3.62ArgPro: 3.62 ± 0.054
3.574ArgGln: 3.574 ± 0.054
5.47ArgArg: 5.47 ± 0.064
3.69ArgSer: 3.69 ± 0.048
3.5ArgThr: 3.5 ± 0.055
5.112ArgVal: 5.112 ± 0.066
1.195ArgTrp: 1.195 ± 0.026
1.905ArgTyr: 1.905 ± 0.039
0.0ArgXaa: 0.0 ± 0.0
Ser
6.978SerAla: 6.978 ± 0.068
0.4SerCys: 0.4 ± 0.016
2.662SerAsp: 2.662 ± 0.09
2.311SerGlu: 2.311 ± 0.042
2.052SerPhe: 2.052 ± 0.047
5.52SerGly: 5.52 ± 0.072
1.365SerHis: 1.365 ± 0.027
2.375SerIle: 2.375 ± 0.044
1.416SerLys: 1.416 ± 0.032
6.125SerLeu: 6.125 ± 0.062
1.284SerMet: 1.284 ± 0.031
1.418SerAsn: 1.418 ± 0.038
3.396SerPro: 3.396 ± 0.055
2.095SerGln: 2.095 ± 0.036
4.011SerArg: 4.011 ± 0.055
3.457SerSer: 3.457 ± 0.07
3.229SerThr: 3.229 ± 0.066
4.11SerVal: 4.11 ± 0.053
0.82SerTrp: 0.82 ± 0.028
1.379SerTyr: 1.379 ± 0.039
0.0SerXaa: 0.0 ± 0.0
Thr
6.452ThrAla: 6.452 ± 0.071
0.357ThrCys: 0.357 ± 0.017
2.487ThrAsp: 2.487 ± 0.037
2.255ThrGlu: 2.255 ± 0.038
1.734ThrPhe: 1.734 ± 0.037
4.827ThrGly: 4.827 ± 0.062
1.241ThrHis: 1.241 ± 0.028
1.948ThrIle: 1.948 ± 0.037
0.996ThrLys: 0.996 ± 0.031
6.707ThrLeu: 6.707 ± 0.075
0.898ThrMet: 0.898 ± 0.026
1.08ThrAsn: 1.08 ± 0.029
3.86ThrPro: 3.86 ± 0.051
2.097ThrGln: 2.097 ± 0.039
3.629ThrArg: 3.629 ± 0.053
2.807ThrSer: 2.807 ± 0.046
2.796ThrThr: 2.796 ± 0.053
4.407ThrVal: 4.407 ± 0.065
0.693ThrTrp: 0.693 ± 0.022
1.149ThrTyr: 1.149 ± 0.027
0.0ThrXaa: 0.0 ± 0.0
Val
9.731ValAla: 9.731 ± 0.09
0.71ValCys: 0.71 ± 0.023
4.161ValAsp: 4.161 ± 0.051
3.795ValGlu: 3.795 ± 0.055
2.733ValPhe: 2.733 ± 0.043
5.602ValGly: 5.602 ± 0.068
1.675ValHis: 1.675 ± 0.039
3.421ValIle: 3.421 ± 0.056
2.211ValLys: 2.211 ± 0.046
8.733ValLeu: 8.733 ± 0.088
1.833ValMet: 1.833 ± 0.039
1.98ValAsn: 1.98 ± 0.041
4.257ValPro: 4.257 ± 0.055
3.107ValGln: 3.107 ± 0.051
5.215ValArg: 5.215 ± 0.063
4.357ValSer: 4.357 ± 0.052
4.197ValThr: 4.197 ± 0.05
6.37ValVal: 6.37 ± 0.078
0.953ValTrp: 0.953 ± 0.027
1.645ValTyr: 1.645 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
1.423TrpAla: 1.423 ± 0.03
0.139TrpCys: 0.139 ± 0.011
0.654TrpAsp: 0.654 ± 0.022
0.443TrpGlu: 0.443 ± 0.016
0.51TrpPhe: 0.51 ± 0.019
0.993TrpGly: 0.993 ± 0.029
0.389TrpHis: 0.389 ± 0.016
0.664TrpIle: 0.664 ± 0.02
0.413TrpLys: 0.413 ± 0.018
2.159TrpLeu: 2.159 ± 0.05
0.398TrpMet: 0.398 ± 0.017
0.414TrpAsn: 0.414 ± 0.019
0.802TrpPro: 0.802 ± 0.026
0.858TrpGln: 0.858 ± 0.028
1.318TrpArg: 1.318 ± 0.031
0.852TrpSer: 0.852 ± 0.026
0.785TrpThr: 0.785 ± 0.024
0.972TrpVal: 0.972 ± 0.028
0.246TrpTrp: 0.246 ± 0.014
0.247TrpTyr: 0.247 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.825TyrAla: 2.825 ± 0.047
0.23TyrCys: 0.23 ± 0.012
1.376TyrAsp: 1.376 ± 0.036
1.182TyrGlu: 1.182 ± 0.032
0.854TyrPhe: 0.854 ± 0.024
2.157TyrGly: 2.157 ± 0.038
0.406TyrHis: 0.406 ± 0.018
0.823TyrIle: 0.823 ± 0.022
0.661TyrLys: 0.661 ± 0.023
2.257TyrLeu: 2.257 ± 0.039
0.426TyrMet: 0.426 ± 0.02
0.624TyrAsn: 0.624 ± 0.02
1.154TyrPro: 1.154 ± 0.027
0.81TyrGln: 0.81 ± 0.022
1.767TyrArg: 1.767 ± 0.04
1.248TyrSer: 1.248 ± 0.027
1.365TyrThr: 1.365 ± 0.038
1.693TyrVal: 1.693 ± 0.035
0.355TyrTrp: 0.355 ± 0.017
0.571TyrTyr: 0.571 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4360 proteins (1468464 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski