Amino acid dipepetide frequency for Gammaproteobacteria bacterium SCGC AG-212-F23

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.524AlaAla: 7.524 ± 0.19
0.979AlaCys: 0.979 ± 0.061
3.935AlaAsp: 3.935 ± 0.101
5.039AlaGlu: 5.039 ± 0.124
3.252AlaPhe: 3.252 ± 0.092
5.206AlaGly: 5.206 ± 0.16
1.797AlaHis: 1.797 ± 0.071
6.582AlaIle: 6.582 ± 0.152
5.495AlaLys: 5.495 ± 0.128
8.705AlaLeu: 8.705 ± 0.169
2.308AlaMet: 2.308 ± 0.085
3.873AlaAsn: 3.873 ± 0.102
2.657AlaPro: 2.657 ± 0.101
2.811AlaGln: 2.811 ± 0.095
3.462AlaArg: 3.462 ± 0.115
4.95AlaSer: 4.95 ± 0.131
5.077AlaThr: 5.077 ± 0.135
5.306AlaVal: 5.306 ± 0.118
1.014AlaTrp: 1.014 ± 0.048
2.492AlaTyr: 2.492 ± 0.082
0.0AlaXaa: 0.0 ± 0.0
Cys
0.867CysAla: 0.867 ± 0.043
0.227CysCys: 0.227 ± 0.026
0.586CysAsp: 0.586 ± 0.041
0.643CysGlu: 0.643 ± 0.041
0.673CysPhe: 0.673 ± 0.048
0.902CysGly: 0.902 ± 0.049
0.379CysHis: 0.379 ± 0.029
0.693CysIle: 0.693 ± 0.04
0.586CysLys: 0.586 ± 0.041
1.094CysLeu: 1.094 ± 0.055
0.287CysMet: 0.287 ± 0.028
0.434CysAsn: 0.434 ± 0.033
0.508CysPro: 0.508 ± 0.045
0.431CysGln: 0.431 ± 0.034
0.508CysArg: 0.508 ± 0.037
0.655CysSer: 0.655 ± 0.034
0.538CysThr: 0.538 ± 0.036
0.72CysVal: 0.72 ± 0.036
0.12CysTrp: 0.12 ± 0.018
0.474CysTyr: 0.474 ± 0.034
0.0CysXaa: 0.0 ± 0.0
Asp
4.055AspAla: 4.055 ± 0.115
0.543AspCys: 0.543 ± 0.034
2.163AspAsp: 2.163 ± 0.072
2.986AspGlu: 2.986 ± 0.096
2.535AspPhe: 2.535 ± 0.088
2.313AspGly: 2.313 ± 0.081
1.256AspHis: 1.256 ± 0.053
3.95AspIle: 3.95 ± 0.089
3.536AspLys: 3.536 ± 0.105
4.825AspLeu: 4.825 ± 0.113
1.087AspMet: 1.087 ± 0.054
2.198AspAsn: 2.198 ± 0.075
1.999AspPro: 1.999 ± 0.069
1.642AspGln: 1.642 ± 0.065
1.969AspArg: 1.969 ± 0.08
2.814AspSer: 2.814 ± 0.089
2.537AspThr: 2.537 ± 0.087
3.272AspVal: 3.272 ± 0.082
0.655AspTrp: 0.655 ± 0.04
1.981AspTyr: 1.981 ± 0.076
0.0AspXaa: 0.0 ± 0.0
Glu
4.197GluAla: 4.197 ± 0.12
0.548GluCys: 0.548 ± 0.038
2.345GluAsp: 2.345 ± 0.077
3.265GluGlu: 3.265 ± 0.122
2.313GluPhe: 2.313 ± 0.074
2.662GluGly: 2.662 ± 0.079
1.371GluHis: 1.371 ± 0.057
4.765GluIle: 4.765 ± 0.126
5.52GluLys: 5.52 ± 0.14
5.607GluLeu: 5.607 ± 0.139
1.473GluMet: 1.473 ± 0.064
3.202GluAsn: 3.202 ± 0.087
1.578GluPro: 1.578 ± 0.073
2.587GluGln: 2.587 ± 0.101
2.789GluArg: 2.789 ± 0.084
2.869GluSer: 2.869 ± 0.095
3.193GluThr: 3.193 ± 0.099
3.202GluVal: 3.202 ± 0.103
0.556GluTrp: 0.556 ± 0.04
1.802GluTyr: 1.802 ± 0.064
0.0GluXaa: 0.0 ± 0.0
Phe
3.512PheAla: 3.512 ± 0.102
0.623PheCys: 0.623 ± 0.04
2.427PheAsp: 2.427 ± 0.076
1.934PheGlu: 1.934 ± 0.061
2.36PhePhe: 2.36 ± 0.084
2.492PheGly: 2.492 ± 0.105
1.094PheHis: 1.094 ± 0.05
3.285PheIle: 3.285 ± 0.105
2.425PheLys: 2.425 ± 0.088
4.755PheLeu: 4.755 ± 0.115
0.987PheMet: 0.987 ± 0.047
2.103PheAsn: 2.103 ± 0.072
1.869PhePro: 1.869 ± 0.068
1.416PheGln: 1.416 ± 0.058
1.453PheArg: 1.453 ± 0.06
3.893PheSer: 3.893 ± 0.105
2.497PheThr: 2.497 ± 0.078
2.537PheVal: 2.537 ± 0.082
0.501PheTrp: 0.501 ± 0.038
1.695PheTyr: 1.695 ± 0.07
0.0PheXaa: 0.0 ± 0.0
Gly
4.496GlyAla: 4.496 ± 0.165
0.768GlyCys: 0.768 ± 0.05
2.704GlyAsp: 2.704 ± 0.08
2.926GlyGlu: 2.926 ± 0.088
2.799GlyPhe: 2.799 ± 0.092
4.11GlyGly: 4.11 ± 0.2
1.408GlyHis: 1.408 ± 0.066
4.962GlyIle: 4.962 ± 0.118
4.361GlyLys: 4.361 ± 0.103
5.949GlyLeu: 5.949 ± 0.14
1.675GlyMet: 1.675 ± 0.069
2.43GlyAsn: 2.43 ± 0.106
1.553GlyPro: 1.553 ± 0.071
2.086GlyGln: 2.086 ± 0.076
2.42GlyArg: 2.42 ± 0.076
3.556GlySer: 3.556 ± 0.171
3.267GlyThr: 3.267 ± 0.245
4.127GlyVal: 4.127 ± 0.134
0.832GlyTrp: 0.832 ± 0.048
2.059GlyTyr: 2.059 ± 0.077
0.0GlyXaa: 0.0 ± 0.0
His
2.211HisAla: 2.211 ± 0.081
0.386HisCys: 0.386 ± 0.033
1.209HisAsp: 1.209 ± 0.05
1.378HisGlu: 1.378 ± 0.063
1.353HisPhe: 1.353 ± 0.064
1.615HisGly: 1.615 ± 0.057
0.974HisHis: 0.974 ± 0.051
1.834HisIle: 1.834 ± 0.067
1.296HisLys: 1.296 ± 0.063
2.729HisLeu: 2.729 ± 0.098
0.603HisMet: 0.603 ± 0.036
0.989HisAsn: 0.989 ± 0.051
1.445HisPro: 1.445 ± 0.065
1.231HisGln: 1.231 ± 0.055
1.094HisArg: 1.094 ± 0.051
1.528HisSer: 1.528 ± 0.064
1.298HisThr: 1.298 ± 0.066
1.463HisVal: 1.463 ± 0.063
0.476HisTrp: 0.476 ± 0.036
1.146HisTyr: 1.146 ± 0.06
0.0HisXaa: 0.0 ± 0.0
Ile
7.08IleAla: 7.08 ± 0.144
0.87IleCys: 0.87 ± 0.046
4.339IleAsp: 4.339 ± 0.106
4.488IleGlu: 4.488 ± 0.114
3.464IlePhe: 3.464 ± 0.108
4.429IleGly: 4.429 ± 0.132
1.919IleHis: 1.919 ± 0.077
5.64IleIle: 5.64 ± 0.146
4.65IleLys: 4.65 ± 0.118
7.529IleLeu: 7.529 ± 0.16
1.54IleMet: 1.54 ± 0.065
3.721IleAsn: 3.721 ± 0.1
3.387IlePro: 3.387 ± 0.104
2.998IleGln: 2.998 ± 0.089
3.215IleArg: 3.215 ± 0.095
4.92IleSer: 4.92 ± 0.107
4.429IleThr: 4.429 ± 0.124
4.498IleVal: 4.498 ± 0.106
0.623IleTrp: 0.623 ± 0.042
2.32IleTyr: 2.32 ± 0.079
0.0IleXaa: 0.0 ± 0.0
Lys
4.324LysAla: 4.324 ± 0.121
0.536LysCys: 0.536 ± 0.038
3.14LysAsp: 3.14 ± 0.113
4.187LysGlu: 4.187 ± 0.114
2.004LysPhe: 2.004 ± 0.077
2.746LysGly: 2.746 ± 0.09
1.777LysHis: 1.777 ± 0.072
5.687LysIle: 5.687 ± 0.132
5.934LysLys: 5.934 ± 0.157
6.118LysLeu: 6.118 ± 0.135
1.789LysMet: 1.789 ± 0.073
4.459LysAsn: 4.459 ± 0.109
2.667LysPro: 2.667 ± 0.086
3.143LysGln: 3.143 ± 0.1
3.058LysArg: 3.058 ± 0.088
4.027LysSer: 4.027 ± 0.12
4.426LysThr: 4.426 ± 0.109
3.04LysVal: 3.04 ± 0.091
0.416LysTrp: 0.416 ± 0.033
1.989LysTyr: 1.989 ± 0.074
0.0LysXaa: 0.0 ± 0.0
Leu
8.945LeuAla: 8.945 ± 0.196
1.189LeuCys: 1.189 ± 0.056
4.773LeuAsp: 4.773 ± 0.105
5.548LeuGlu: 5.548 ± 0.137
4.685LeuPhe: 4.685 ± 0.134
6.32LeuGly: 6.32 ± 0.139
2.739LeuHis: 2.739 ± 0.09
7.265LeuIle: 7.265 ± 0.152
6.54LeuLys: 6.54 ± 0.158
11.085LeuLeu: 11.085 ± 0.257
2.34LeuMet: 2.34 ± 0.073
4.723LeuAsn: 4.723 ± 0.121
4.967LeuPro: 4.967 ± 0.116
5.109LeuGln: 5.109 ± 0.14
4.718LeuArg: 4.718 ± 0.109
7.205LeuSer: 7.205 ± 0.135
6.143LeuThr: 6.143 ± 0.12
5.695LeuVal: 5.695 ± 0.128
1.034LeuTrp: 1.034 ± 0.053
2.746LeuTyr: 2.746 ± 0.097
0.0LeuXaa: 0.0 ± 0.0
Met
1.956MetAla: 1.956 ± 0.067
0.169MetCys: 0.169 ± 0.023
1.121MetAsp: 1.121 ± 0.052
1.184MetGlu: 1.184 ± 0.057
0.745MetPhe: 0.745 ± 0.046
1.361MetGly: 1.361 ± 0.069
0.71MetHis: 0.71 ± 0.039
1.772MetIle: 1.772 ± 0.074
1.428MetLys: 1.428 ± 0.051
2.597MetLeu: 2.597 ± 0.088
0.631MetMet: 0.631 ± 0.042
1.139MetAsn: 1.139 ± 0.051
1.214MetPro: 1.214 ± 0.059
1.495MetGln: 1.495 ± 0.057
1.288MetArg: 1.288 ± 0.057
1.612MetSer: 1.612 ± 0.071
1.66MetThr: 1.66 ± 0.059
1.388MetVal: 1.388 ± 0.06
0.157MetTrp: 0.157 ± 0.019
0.533MetTyr: 0.533 ± 0.038
0.0MetXaa: 0.0 ± 0.0
Asn
3.831AsnAla: 3.831 ± 0.111
0.446AsnCys: 0.446 ± 0.034
2.412AsnAsp: 2.412 ± 0.083
2.537AsnGlu: 2.537 ± 0.089
1.931AsnPhe: 1.931 ± 0.078
2.417AsnGly: 2.417 ± 0.111
1.316AsnHis: 1.316 ± 0.063
3.99AsnIle: 3.99 ± 0.095
3.235AsnLys: 3.235 ± 0.107
4.237AsnLeu: 4.237 ± 0.101
1.064AsnMet: 1.064 ± 0.053
2.789AsnAsn: 2.789 ± 0.124
2.587AsnPro: 2.587 ± 0.08
2.216AsnGln: 2.216 ± 0.075
1.971AsnArg: 1.971 ± 0.069
2.866AsnSer: 2.866 ± 0.129
3.055AsnThr: 3.055 ± 0.119
2.574AsnVal: 2.574 ± 0.097
0.528AsnTrp: 0.528 ± 0.037
1.675AsnTyr: 1.675 ± 0.065
0.0AsnXaa: 0.0 ± 0.0
Pro
3.34ProAla: 3.34 ± 0.101
0.389ProCys: 0.389 ± 0.031
2.255ProAsp: 2.255 ± 0.074
3.06ProGlu: 3.06 ± 0.099
1.755ProPhe: 1.755 ± 0.064
2.457ProGly: 2.457 ± 0.084
1.084ProHis: 1.084 ± 0.056
2.854ProIle: 2.854 ± 0.094
2.28ProLys: 2.28 ± 0.074
4.321ProLeu: 4.321 ± 0.107
0.962ProMet: 0.962 ± 0.049
1.812ProAsn: 1.812 ± 0.069
1.882ProPro: 1.882 ± 0.121
1.632ProGln: 1.632 ± 0.063
1.68ProArg: 1.68 ± 0.072
2.572ProSer: 2.572 ± 0.1
2.592ProThr: 2.592 ± 0.083
3.16ProVal: 3.16 ± 0.105
0.466ProTrp: 0.466 ± 0.035
1.416ProTyr: 1.416 ± 0.068
0.0ProXaa: 0.0 ± 0.0
Gln
3.407GlnAla: 3.407 ± 0.113
0.476GlnCys: 0.476 ± 0.033
1.752GlnAsp: 1.752 ± 0.075
2.388GlnGlu: 2.388 ± 0.089
1.735GlnPhe: 1.735 ± 0.062
2.435GlnGly: 2.435 ± 0.094
1.341GlnHis: 1.341 ± 0.061
2.836GlnIle: 2.836 ± 0.09
3.173GlnLys: 3.173 ± 0.103
4.725GlnLeu: 4.725 ± 0.115
0.959GlnMet: 0.959 ± 0.046
2.056GlnAsn: 2.056 ± 0.075
1.727GlnPro: 1.727 ± 0.077
2.844GlnGln: 2.844 ± 0.121
2.083GlnArg: 2.083 ± 0.079
2.4GlnSer: 2.4 ± 0.085
2.343GlnThr: 2.343 ± 0.079
2.203GlnVal: 2.203 ± 0.084
0.533GlnTrp: 0.533 ± 0.04
1.445GlnTyr: 1.445 ± 0.067
0.0GlnXaa: 0.0 ± 0.0
Arg
3.302ArgAla: 3.302 ± 0.099
0.406ArgCys: 0.406 ± 0.031
2.365ArgAsp: 2.365 ± 0.081
3.003ArgGlu: 3.003 ± 0.095
2.106ArgPhe: 2.106 ± 0.091
2.398ArgGly: 2.398 ± 0.083
1.109ArgHis: 1.109 ± 0.058
3.247ArgIle: 3.247 ± 0.094
2.931ArgLys: 2.931 ± 0.092
4.645ArgLeu: 4.645 ± 0.111
1.126ArgMet: 1.126 ± 0.052
1.917ArgAsn: 1.917 ± 0.064
1.483ArgPro: 1.483 ± 0.06
1.819ArgGln: 1.819 ± 0.079
2.086ArgArg: 2.086 ± 0.081
2.298ArgSer: 2.298 ± 0.086
2.001ArgThr: 2.001 ± 0.077
2.719ArgVal: 2.719 ± 0.079
0.583ArgTrp: 0.583 ± 0.038
1.782ArgTyr: 1.782 ± 0.068
0.0ArgXaa: 0.0 ± 0.0
Ser
5.393SerAla: 5.393 ± 0.139
0.845SerCys: 0.845 ± 0.053
2.839SerAsp: 2.839 ± 0.079
3.197SerGlu: 3.197 ± 0.091
2.761SerPhe: 2.761 ± 0.085
4.424SerGly: 4.424 ± 0.24
1.779SerHis: 1.779 ± 0.07
4.461SerIle: 4.461 ± 0.12
3.417SerLys: 3.417 ± 0.113
6.746SerLeu: 6.746 ± 0.13
1.568SerMet: 1.568 ± 0.064
2.881SerAsn: 2.881 ± 0.124
2.717SerPro: 2.717 ± 0.078
2.472SerGln: 2.472 ± 0.091
2.482SerArg: 2.482 ± 0.09
4.994SerSer: 4.994 ± 0.227
4.067SerThr: 4.067 ± 0.174
4.03SerVal: 4.03 ± 0.112
0.718SerTrp: 0.718 ± 0.052
2.138SerTyr: 2.138 ± 0.083
0.0SerXaa: 0.0 ± 0.0
Thr
5.49ThrAla: 5.49 ± 0.176
0.608ThrCys: 0.608 ± 0.04
2.724ThrAsp: 2.724 ± 0.084
2.946ThrGlu: 2.946 ± 0.098
2.288ThrPhe: 2.288 ± 0.079
3.963ThrGly: 3.963 ± 0.171
1.687ThrHis: 1.687 ± 0.076
4.207ThrIle: 4.207 ± 0.119
2.891ThrLys: 2.891 ± 0.096
6.796ThrLeu: 6.796 ± 0.167
1.234ThrMet: 1.234 ± 0.055
2.333ThrAsn: 2.333 ± 0.107
3.1ThrPro: 3.1 ± 0.116
2.53ThrGln: 2.53 ± 0.082
2.283ThrArg: 2.283 ± 0.072
3.99ThrSer: 3.99 ± 0.179
4.601ThrThr: 4.601 ± 0.208
3.873ThrVal: 3.873 ± 0.123
0.578ThrTrp: 0.578 ± 0.039
1.617ThrTyr: 1.617 ± 0.066
0.0ThrXaa: 0.0 ± 0.0
Val
5.136ValAla: 5.136 ± 0.127
0.71ValCys: 0.71 ± 0.039
3.14ValAsp: 3.14 ± 0.093
3.098ValGlu: 3.098 ± 0.084
2.654ValPhe: 2.654 ± 0.088
3.626ValGly: 3.626 ± 0.107
1.226ValHis: 1.226 ± 0.055
4.957ValIle: 4.957 ± 0.117
3.616ValLys: 3.616 ± 0.098
6.25ValLeu: 6.25 ± 0.156
1.57ValMet: 1.57 ± 0.062
2.854ValAsn: 2.854 ± 0.092
2.657ValPro: 2.657 ± 0.084
1.894ValGln: 1.894 ± 0.075
2.512ValArg: 2.512 ± 0.083
4.14ValSer: 4.14 ± 0.098
3.89ValThr: 3.89 ± 0.123
4.284ValVal: 4.284 ± 0.125
0.551ValTrp: 0.551 ± 0.043
1.862ValTyr: 1.862 ± 0.072
0.0ValXaa: 0.0 ± 0.0
Trp
0.663TrpAla: 0.663 ± 0.048
0.125TrpCys: 0.125 ± 0.017
0.481TrpAsp: 0.481 ± 0.033
0.488TrpGlu: 0.488 ± 0.03
0.553TrpPhe: 0.553 ± 0.041
0.618TrpGly: 0.618 ± 0.04
0.324TrpHis: 0.324 ± 0.028
0.87TrpIle: 0.87 ± 0.047
0.573TrpLys: 0.573 ± 0.042
1.333TrpLeu: 1.333 ± 0.064
0.294TrpMet: 0.294 ± 0.025
0.528TrpAsn: 0.528 ± 0.035
0.446TrpPro: 0.446 ± 0.04
0.735TrpGln: 0.735 ± 0.05
0.548TrpArg: 0.548 ± 0.036
0.665TrpSer: 0.665 ± 0.04
0.493TrpThr: 0.493 ± 0.04
0.72TrpVal: 0.72 ± 0.044
0.152TrpTrp: 0.152 ± 0.023
0.336TrpTyr: 0.336 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.736TyrAla: 2.736 ± 0.089
0.481TyrCys: 0.481 ± 0.035
1.605TyrAsp: 1.605 ± 0.062
1.55TyrGlu: 1.55 ± 0.063
1.892TyrPhe: 1.892 ± 0.08
1.994TyrGly: 1.994 ± 0.077
0.969TyrHis: 0.969 ± 0.052
2.203TyrIle: 2.203 ± 0.076
1.58TyrLys: 1.58 ± 0.082
3.718TyrLeu: 3.718 ± 0.111
0.663TyrMet: 0.663 ± 0.045
1.239TyrAsn: 1.239 ± 0.061
1.528TyrPro: 1.528 ± 0.06
1.769TyrGln: 1.769 ± 0.068
1.707TyrArg: 1.707 ± 0.063
1.976TyrSer: 1.976 ± 0.078
1.63TyrThr: 1.63 ± 0.055
1.814TyrVal: 1.814 ± 0.061
0.454TyrTrp: 0.454 ± 0.037
1.401TyrTyr: 1.401 ± 0.069
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1263 proteins (401252 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski