Amino acid dipepetide frequency for Paenibacillus macerans (Bacillus macerans)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	10.912AlaAla: 10.912 ± 0.124	0.786AlaCys: 0.786 ± 0.02	4.745AlaAsp: 4.745 ± 0.056	6.548AlaGlu: 6.548 ± 0.075	3.488AlaPhe: 3.488 ± 0.049	8.316AlaGly: 8.316 ± 0.081	1.387AlaHis: 1.387 ± 0.025	5.048AlaIle: 5.048 ± 0.062	4.598AlaLys: 4.598 ± 0.055	9.064AlaLeu: 9.064 ± 0.074	2.346AlaMet: 2.346 ± 0.037	2.719AlaAsn: 2.719 ± 0.041	3.094AlaPro: 3.094 ± 0.056	2.684AlaGln: 2.684 ± 0.04	3.917AlaArg: 3.917 ± 0.053	5.046AlaSer: 5.046 ± 0.047	3.325AlaThr: 3.325 ± 0.056	7.19AlaVal: 7.19 ± 0.064	1.119AlaTrp: 1.119 ± 0.024	2.831AlaTyr: 2.831 ± 0.034	0.0AlaXaa: 0.0 ± 0.0
Cys	0.602CysAla: 0.602 ± 0.019	0.129CysCys: 0.129 ± 0.007	0.387CysAsp: 0.387 ± 0.015	0.443CysGlu: 0.443 ± 0.016	0.355CysPhe: 0.355 ± 0.014	0.872CysGly: 0.872 ± 0.023	0.177CysHis: 0.177 ± 0.009	0.487CysIle: 0.487 ± 0.015	0.341CysLys: 0.341 ± 0.015	0.857CysLeu: 0.857 ± 0.021	0.213CysMet: 0.213 ± 0.01	0.271CysAsn: 0.271 ± 0.011	0.39CysPro: 0.39 ± 0.016	0.238CysGln: 0.238 ± 0.011	0.549CysArg: 0.549 ± 0.017	0.588CysSer: 0.588 ± 0.016	0.41CysThr: 0.41 ± 0.015	0.513CysVal: 0.513 ± 0.015	0.085CysTrp: 0.085 ± 0.007	0.282CysTyr: 0.282 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	3.989AspAla: 3.989 ± 0.054	0.43AspCys: 0.43 ± 0.016	2.393AspAsp: 2.393 ± 0.042	3.759AspGlu: 3.759 ± 0.046	2.229AspPhe: 2.229 ± 0.034	4.219AspGly: 4.219 ± 0.059	1.017AspHis: 1.017 ± 0.022	3.388AspIle: 3.388 ± 0.043	2.667AspLys: 2.667 ± 0.044	4.847AspLeu: 4.847 ± 0.049	1.308AspMet: 1.308 ± 0.026	1.721AspAsn: 1.721 ± 0.03	2.526AspPro: 2.526 ± 0.035	1.73AspGln: 1.73 ± 0.028	2.938AspArg: 2.938 ± 0.038	2.559AspSer: 2.559 ± 0.039	2.387AspThr: 2.387 ± 0.036	3.378AspVal: 3.378 ± 0.045	0.825AspTrp: 0.825 ± 0.02	2.128AspTyr: 2.128 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	6.574GluAla: 6.574 ± 0.071	0.417GluCys: 0.417 ± 0.015	3.086GluAsp: 3.086 ± 0.038	5.308GluGlu: 5.308 ± 0.072	2.291GluPhe: 2.291 ± 0.036	4.417GluGly: 4.417 ± 0.048	1.501GluHis: 1.501 ± 0.032	4.357GluIle: 4.357 ± 0.052	3.88GluLys: 3.88 ± 0.049	7.36GluLeu: 7.36 ± 0.061	1.983GluMet: 1.983 ± 0.037	2.525GluAsn: 2.525 ± 0.038	2.568GluPro: 2.568 ± 0.04	3.562GluGln: 3.562 ± 0.045	4.438GluArg: 4.438 ± 0.055	3.252GluSer: 3.252 ± 0.048	3.489GluThr: 3.489 ± 0.046	4.331GluVal: 4.331 ± 0.051	0.91GluTrp: 0.91 ± 0.022	2.151GluTyr: 2.151 ± 0.031	0.0GluXaa: 0.0 ± 0.0
Phe	3.713PheAla: 3.713 ± 0.045	0.423PheCys: 0.423 ± 0.015	2.229PheAsp: 2.229 ± 0.033	2.372PheGlu: 2.372 ± 0.034	1.899PhePhe: 1.899 ± 0.034	3.397PheGly: 3.397 ± 0.047	0.871PheHis: 0.871 ± 0.023	2.782PheIle: 2.782 ± 0.041	1.949PheLys: 1.949 ± 0.03	3.981PheLeu: 3.981 ± 0.051	1.127PheMet: 1.127 ± 0.023	1.586PheAsn: 1.586 ± 0.032	1.73PhePro: 1.73 ± 0.028	1.356PheGln: 1.356 ± 0.025	2.246PheArg: 2.246 ± 0.033	2.744PheSer: 2.744 ± 0.043	2.409PheThr: 2.409 ± 0.037	2.952PheVal: 2.952 ± 0.044	0.562PheTrp: 0.562 ± 0.016	1.543PheTyr: 1.543 ± 0.031	0.0PheXaa: 0.0 ± 0.0
Gly	6.415GlyAla: 6.415 ± 0.078	0.773GlyCys: 0.773 ± 0.021	3.7GlyAsp: 3.7 ± 0.049	5.114GlyGlu: 5.114 ± 0.05	3.324GlyPhe: 3.324 ± 0.046	6.593GlyGly: 6.593 ± 0.088	1.494GlyHis: 1.494 ± 0.032	5.481GlyIle: 5.481 ± 0.057	4.722GlyLys: 4.722 ± 0.048	7.698GlyLeu: 7.698 ± 0.068	2.295GlyMet: 2.295 ± 0.033	2.882GlyAsn: 2.882 ± 0.043	2.314GlyPro: 2.314 ± 0.04	2.793GlyGln: 2.793 ± 0.037	4.067GlyArg: 4.067 ± 0.047	4.753GlySer: 4.753 ± 0.052	4.41GlyThr: 4.41 ± 0.062	5.354GlyVal: 5.354 ± 0.059	1.115GlyTrp: 1.115 ± 0.028	3.045GlyTyr: 3.045 ± 0.038	0.0GlyXaa: 0.0 ± 0.0
His	1.503HisAla: 1.503 ± 0.028	0.198HisCys: 0.198 ± 0.009	0.933HisAsp: 0.933 ± 0.023	1.268HisGlu: 1.268 ± 0.027	1.02HisPhe: 1.02 ± 0.024	1.477HisGly: 1.477 ± 0.031	0.534HisHis: 0.534 ± 0.018	1.291HisIle: 1.291 ± 0.027	0.79HisLys: 0.79 ± 0.021	2.07HisLeu: 2.07 ± 0.043	0.496HisMet: 0.496 ± 0.016	0.63HisAsn: 0.63 ± 0.017	1.219HisPro: 1.219 ± 0.027	0.688HisGln: 0.688 ± 0.018	1.103HisArg: 1.103 ± 0.023	1.03HisSer: 1.03 ± 0.024	0.988HisThr: 0.988 ± 0.021	1.262HisVal: 1.262 ± 0.026	0.303HisTrp: 0.303 ± 0.012	0.825HisTyr: 0.825 ± 0.019	0.0HisXaa: 0.0 ± 0.0
Ile	6.008IleAla: 6.008 ± 0.057	0.591IleCys: 0.591 ± 0.017	3.411IleAsp: 3.411 ± 0.039	4.041IleGlu: 4.041 ± 0.051	2.462IlePhe: 2.462 ± 0.038	5.29IleGly: 5.29 ± 0.061	1.317IleHis: 1.317 ± 0.026	3.747IleIle: 3.747 ± 0.057	2.964IleLys: 2.964 ± 0.04	5.581IleLeu: 5.581 ± 0.069	1.506IleMet: 1.506 ± 0.028	2.223IleAsn: 2.223 ± 0.033	3.094IlePro: 3.094 ± 0.046	2.127IleGln: 2.127 ± 0.033	3.781IleArg: 3.781 ± 0.054	4.209IleSer: 4.209 ± 0.048	3.495IleThr: 3.495 ± 0.044	4.971IleVal: 4.971 ± 0.06	0.67IleTrp: 0.67 ± 0.019	2.045IleTyr: 2.045 ± 0.033	0.0IleXaa: 0.0 ± 0.0
Lys	4.477LysAla: 4.477 ± 0.051	0.297LysCys: 0.297 ± 0.012	2.663LysAsp: 2.663 ± 0.037	4.197LysGlu: 4.197 ± 0.061	1.611LysPhe: 1.611 ± 0.029	3.458LysGly: 3.458 ± 0.047	1.044LysHis: 1.044 ± 0.024	3.28LysIle: 3.28 ± 0.046	3.21LysLys: 3.21 ± 0.052	5.442LysLeu: 5.442 ± 0.053	1.554LysMet: 1.554 ± 0.025	2.154LysAsn: 2.154 ± 0.037	2.278LysPro: 2.278 ± 0.03	2.377LysGln: 2.377 ± 0.038	3.036LysArg: 3.036 ± 0.044	2.883LysSer: 2.883 ± 0.038	2.891LysThr: 2.891 ± 0.046	3.513LysVal: 3.513 ± 0.044	0.752LysTrp: 0.752 ± 0.021	1.944LysTyr: 1.944 ± 0.034	0.0LysXaa: 0.0 ± 0.001
Leu	9.214LeuAla: 9.214 ± 0.077	0.859LeuCys: 0.859 ± 0.02	5.218LeuAsp: 5.218 ± 0.055	6.538LeuGlu: 6.538 ± 0.063	4.563LeuPhe: 4.563 ± 0.06	7.165LeuGly: 7.165 ± 0.068	1.978LeuHis: 1.978 ± 0.035	6.426LeuIle: 6.426 ± 0.074	5.52LeuLys: 5.52 ± 0.052	11.309LeuLeu: 11.309 ± 0.115	2.476LeuMet: 2.476 ± 0.036	3.836LeuAsn: 3.836 ± 0.047	4.828LeuPro: 4.828 ± 0.048	3.913LeuGln: 3.913 ± 0.052	5.518LeuArg: 5.518 ± 0.065	6.822LeuSer: 6.822 ± 0.067	5.55LeuThr: 5.55 ± 0.054	6.266LeuVal: 6.266 ± 0.06	1.05LeuTrp: 1.05 ± 0.028	3.266LeuTyr: 3.266 ± 0.039	0.0LeuXaa: 0.0 ± 0.0
Met	2.278MetAla: 2.278 ± 0.032	0.186MetCys: 0.186 ± 0.009	1.403MetAsp: 1.403 ± 0.026	1.872MetGlu: 1.872 ± 0.032	0.994MetPhe: 0.994 ± 0.025	1.696MetGly: 1.696 ± 0.031	0.412MetHis: 0.412 ± 0.015	1.83MetIle: 1.83 ± 0.033	1.852MetLys: 1.852 ± 0.033	2.9MetLeu: 2.9 ± 0.043	0.85MetMet: 0.85 ± 0.023	1.406MetAsn: 1.406 ± 0.024	1.084MetPro: 1.084 ± 0.025	0.924MetGln: 0.924 ± 0.025	1.308MetArg: 1.308 ± 0.026	1.647MetSer: 1.647 ± 0.033	1.515MetThr: 1.515 ± 0.027	1.678MetVal: 1.678 ± 0.032	0.208MetTrp: 0.208 ± 0.009	0.696MetTyr: 0.696 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	2.928AsnAla: 2.928 ± 0.04	0.257AsnCys: 0.257 ± 0.013	1.771AsnAsp: 1.771 ± 0.029	2.454AsnGlu: 2.454 ± 0.034	1.347AsnPhe: 1.347 ± 0.025	3.239AsnGly: 3.239 ± 0.049	0.749AsnHis: 0.749 ± 0.022	2.353AsnIle: 2.353 ± 0.041	1.901AsnLys: 1.901 ± 0.035	3.398AsnLeu: 3.398 ± 0.045	0.962AsnMet: 0.962 ± 0.019	1.507AsnAsn: 1.507 ± 0.034	2.076AsnPro: 2.076 ± 0.034	1.351AsnGln: 1.351 ± 0.03	2.153AsnArg: 2.153 ± 0.035	2.032AsnSer: 2.032 ± 0.031	1.876AsnThr: 1.876 ± 0.036	2.532AsnVal: 2.532 ± 0.038	0.534AsnTrp: 0.534 ± 0.017	1.425AsnTyr: 1.425 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	4.084ProAla: 4.084 ± 0.074	0.268ProCys: 0.268 ± 0.011	2.739ProAsp: 2.739 ± 0.036	3.787ProGlu: 3.787 ± 0.048	1.949ProPhe: 1.949 ± 0.031	3.527ProGly: 3.527 ± 0.051	0.85ProHis: 0.85 ± 0.02	2.244ProIle: 2.244 ± 0.036	1.878ProLys: 1.878 ± 0.033	4.267ProLeu: 4.267 ± 0.044	0.914ProMet: 0.914 ± 0.023	1.486ProAsn: 1.486 ± 0.026	1.472ProPro: 1.472 ± 0.031	1.534ProGln: 1.534 ± 0.028	1.725ProArg: 1.725 ± 0.031	2.44ProSer: 2.44 ± 0.037	1.715ProThr: 1.715 ± 0.034	3.376ProVal: 3.376 ± 0.045	0.55ProTrp: 0.55 ± 0.018	1.634ProTyr: 1.634 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	3.593GlnAla: 3.593 ± 0.039	0.194GlnCys: 0.194 ± 0.01	1.662GlnAsp: 1.662 ± 0.031	2.488GlnGlu: 2.488 ± 0.033	1.393GlnPhe: 1.393 ± 0.025	2.681GlnGly: 2.681 ± 0.034	0.732GlnHis: 0.732 ± 0.019	2.354GlnIle: 2.354 ± 0.038	1.935GlnLys: 1.935 ± 0.033	3.734GlnLeu: 3.734 ± 0.044	1.035GlnMet: 1.035 ± 0.022	1.413GlnAsn: 1.413 ± 0.027	1.535GlnPro: 1.535 ± 0.033	1.65GlnGln: 1.65 ± 0.032	1.934GlnArg: 1.934 ± 0.032	2.046GlnSer: 2.046 ± 0.037	1.974GlnThr: 1.974 ± 0.037	2.405GlnVal: 2.405 ± 0.038	0.439GlnTrp: 0.439 ± 0.014	1.235GlnTyr: 1.235 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	3.715ArgAla: 3.715 ± 0.049	0.466ArgCys: 0.466 ± 0.017	2.577ArgAsp: 2.577 ± 0.045	4.308ArgGlu: 4.308 ± 0.055	2.492ArgPhe: 2.492 ± 0.035	3.402ArgGly: 3.402 ± 0.055	1.218ArgHis: 1.218 ± 0.026	3.724ArgIle: 3.724 ± 0.041	3.281ArgLys: 3.281 ± 0.041	5.995ArgLeu: 5.995 ± 0.064	1.689ArgMet: 1.689 ± 0.032	1.993ArgAsn: 1.993 ± 0.031	1.991ArgPro: 1.991 ± 0.031	2.301ArgGln: 2.301 ± 0.038	3.413ArgArg: 3.413 ± 0.053	3.199ArgSer: 3.199 ± 0.04	2.712ArgThr: 2.712 ± 0.03	3.268ArgVal: 3.268 ± 0.04	0.702ArgTrp: 0.702 ± 0.019	2.033ArgTyr: 2.033 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	5.221SerAla: 5.221 ± 0.063	0.473SerCys: 0.473 ± 0.016	2.832SerAsp: 2.832 ± 0.043	3.515SerGlu: 3.515 ± 0.043	2.855SerPhe: 2.855 ± 0.04	5.854SerGly: 5.854 ± 0.062	1.102SerHis: 1.102 ± 0.023	3.735SerIle: 3.735 ± 0.046	2.912SerLys: 2.912 ± 0.044	6.2SerLeu: 6.2 ± 0.058	1.562SerMet: 1.562 ± 0.026	1.965SerAsn: 1.965 ± 0.037	2.596SerPro: 2.596 ± 0.039	1.807SerGln: 1.807 ± 0.034	3.292SerArg: 3.292 ± 0.046	3.841SerSer: 3.841 ± 0.058	2.706SerThr: 2.706 ± 0.037	4.191SerVal: 4.191 ± 0.048	0.789SerTrp: 0.789 ± 0.022	2.115SerTyr: 2.115 ± 0.033	0.0SerXaa: 0.0 ± 0.0
Thr	4.896ThrAla: 4.896 ± 0.058	0.362ThrCys: 0.362 ± 0.014	2.654ThrAsp: 2.654 ± 0.038	3.061ThrGlu: 3.061 ± 0.042	2.219ThrPhe: 2.219 ± 0.037	4.634ThrGly: 4.634 ± 0.052	0.898ThrHis: 0.898 ± 0.021	3.285ThrIle: 3.285 ± 0.044	2.287ThrLys: 2.287 ± 0.034	5.353ThrLeu: 5.353 ± 0.054	1.258ThrMet: 1.258 ± 0.029	1.714ThrAsn: 1.714 ± 0.031	2.434ThrPro: 2.434 ± 0.036	1.381ThrGln: 1.381 ± 0.027	2.32ThrArg: 2.32 ± 0.034	2.923ThrSer: 2.923 ± 0.041	2.401ThrThr: 2.401 ± 0.046	4.398ThrVal: 4.398 ± 0.05	0.648ThrTrp: 0.648 ± 0.018	1.809ThrTyr: 1.809 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	5.352ValAla: 5.352 ± 0.054	0.677ValCys: 0.677 ± 0.02	3.429ValAsp: 3.429 ± 0.046	4.162ValGlu: 4.162 ± 0.041	3.076ValPhe: 3.076 ± 0.04	4.368ValGly: 4.368 ± 0.051	1.38ValHis: 1.38 ± 0.026	4.688ValIle: 4.688 ± 0.05	3.907ValLys: 3.907 ± 0.052	7.308ValLeu: 7.308 ± 0.075	1.938ValMet: 1.938 ± 0.03	2.778ValAsn: 2.778 ± 0.041	3.245ValPro: 3.245 ± 0.045	2.396ValGln: 2.396 ± 0.036	3.744ValArg: 3.744 ± 0.038	4.665ValSer: 4.665 ± 0.049	4.123ValThr: 4.123 ± 0.053	4.838ValVal: 4.838 ± 0.053	0.907ValTrp: 0.907 ± 0.02	2.497ValTyr: 2.497 ± 0.034	0.0ValXaa: 0.0 ± 0.0
Trp	0.862TrpAla: 0.862 ± 0.021	0.099TrpCys: 0.099 ± 0.007	0.665TrpAsp: 0.665 ± 0.019	0.853TrpGlu: 0.853 ± 0.024	0.577TrpPhe: 0.577 ± 0.016	0.908TrpGly: 0.908 ± 0.023	0.235TrpHis: 0.235 ± 0.012	0.897TrpIle: 0.897 ± 0.023	0.744TrpLys: 0.744 ± 0.018	1.466TrpLeu: 1.466 ± 0.03	0.392TrpMet: 0.392 ± 0.013	0.658TrpAsn: 0.658 ± 0.018	0.422TrpPro: 0.422 ± 0.015	0.482TrpGln: 0.482 ± 0.014	0.682TrpArg: 0.682 ± 0.022	0.82TrpSer: 0.82 ± 0.021	0.694TrpThr: 0.694 ± 0.021	0.777TrpVal: 0.777 ± 0.02	0.185TrpTrp: 0.185 ± 0.011	0.4TrpTyr: 0.4 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.827TyrAla: 2.827 ± 0.041	0.305TyrCys: 0.305 ± 0.012	1.941TyrAsp: 1.941 ± 0.033	2.311TyrGlu: 2.311 ± 0.037	1.727TyrPhe: 1.727 ± 0.03	2.826TyrGly: 2.826 ± 0.038	0.734TyrHis: 0.734 ± 0.019	2.135TyrIle: 2.135 ± 0.032	1.725TyrLys: 1.725 ± 0.032	3.482TyrLeu: 3.482 ± 0.042	0.833TyrMet: 0.833 ± 0.02	1.379TyrAsn: 1.379 ± 0.031	1.546TyrPro: 1.546 ± 0.027	1.139TyrGln: 1.139 ± 0.026	2.316TyrArg: 2.316 ± 0.039	2.066TyrSer: 2.066 ± 0.032	1.865TyrThr: 1.865 ± 0.035	2.284TyrVal: 2.284 ± 0.036	0.464TyrTrp: 0.464 ± 0.016	1.421TyrTyr: 1.421 ± 0.031	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6551 proteins (2036740 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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