Ornithorhynchus anatinus (Duckbill platypus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amniota; Mammalia; Prototheria; Monotremata; Ornithorhynchidae;

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 32824 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F7FBF3|F7FBF3_ORNAN Nuclear factor related to kappaB binding protein OS=Ornithorhynchus anatinus OX=9258 GN=NFRKB PE=4 SV=2
MM1 pKa = 7.45LSSSAILDD9 pKa = 4.05HH10 pKa = 7.09PPSPMDD16 pKa = 3.15TSEE19 pKa = 4.26LHH21 pKa = 6.18FAPEE25 pKa = 4.11PGAGLGLDD33 pKa = 4.42LAASHH38 pKa = 7.11LDD40 pKa = 3.39GMDD43 pKa = 2.95WLEE46 pKa = 4.35LSGGPAASLAPLGAAAPSLFSTDD69 pKa = 3.84FLDD72 pKa = 4.44GHH74 pKa = 6.85DD75 pKa = 5.24LQLHH79 pKa = 6.18WDD81 pKa = 3.6SCLL84 pKa = 3.37

Molecular weight: 8.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I8ND41|A0A6I8ND41_ORNAN Non-specific serine/threonine protein kinase OS=Ornithorhynchus anatinus OX=9258 PE=3 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

17390

15434

32824

22389980

22

8244

682.1

75.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.241 ± 0.014

2.103 ± 0.011

4.893 ± 0.009

7.057 ± 0.018

3.621 ± 0.009

7.075 ± 0.019

2.442 ± 0.006

4.214 ± 0.012

5.506 ± 0.015

9.871 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.995 ± 0.005

3.497 ± 0.009

6.737 ± 0.022

4.542 ± 0.011

6.171 ± 0.015

8.134 ± 0.017

5.111 ± 0.01

6.037 ± 0.01

1.209 ± 0.004

2.543 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski