Acaryochloris phage A-HIS1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A120N0C0|A0A120N0C0_9CAUD Uncharacterized protein OS=Acaryochloris phage A-HIS1 OX=645116 GN=AHIS1_p091 PE=4 SV=1
MM1 pKa = 7.24VLPHH5 pKa = 6.95AAGSNISNRR14 pKa = 11.84GTIALPQNNNNGIVTGFDD32 pKa = 2.7IRR34 pKa = 11.84SRR36 pKa = 11.84QAIVNLDD43 pKa = 3.4GSGEE47 pKa = 4.35SVRR50 pKa = 11.84VAVPIGQIVVPGQIAQITGSTTGGAFTGSFVFNTRR85 pKa = 11.84RR86 pKa = 11.84NRR88 pKa = 11.84IAAGTNGTVNVGSNAASANTPASCDD113 pKa = 3.22ISQLAPTSPASNGGNEE129 pKa = 4.08GDD131 pKa = 4.1EE132 pKa = 4.57CPSGYY137 pKa = 10.18VPNCGAPGTPEE148 pKa = 3.75EE149 pKa = 4.39NQCFGCKK156 pKa = 9.55PEE158 pKa = 4.27EE159 pKa = 4.36DD160 pKa = 4.05EE161 pKa = 3.99EE162 pKa = 5.06TEE164 pKa = 4.19FEE166 pKa = 4.3EE167 pKa = 5.53RR168 pKa = 11.84PNIPGNPGDD177 pKa = 3.89GCGGLNDD184 pKa = 3.56SCDD187 pKa = 3.3WYY189 pKa = 10.42IVASEE194 pKa = 4.11AAAVCPTQMINKK206 pKa = 8.94GFATIDD212 pKa = 3.09GVTRR216 pKa = 11.84VLCCGGEE223 pKa = 3.96DD224 pKa = 3.66RR225 pKa = 11.84PPGDD229 pKa = 3.5GCSWQQLSCGLFYY242 pKa = 11.09SPNPDD247 pKa = 3.52DD248 pKa = 4.64QFCEE252 pKa = 4.17VNPTCGADD260 pKa = 3.15GRR262 pKa = 11.84RR263 pKa = 11.84VYY265 pKa = 11.05DD266 pKa = 3.6SLSDD270 pKa = 4.04CEE272 pKa = 5.24ADD274 pKa = 4.09LPTPGLCEE282 pKa = 3.6TLYY285 pKa = 11.68KK286 pKa = 10.13MFLKK290 pKa = 10.29FQSGPFPAVEE300 pKa = 4.47PEE302 pKa = 3.98GDD304 pKa = 3.3WFFDD308 pKa = 3.92GPVTNIRR315 pKa = 11.84VEE317 pKa = 4.25EE318 pKa = 4.13TGGVAGRR325 pKa = 11.84PIVFYY330 pKa = 10.57DD331 pKa = 3.53RR332 pKa = 11.84PGFGTDD338 pKa = 4.62FIASTGVGFSPGAVPFALTEE358 pKa = 3.89QGPLLRR364 pKa = 11.84VDD366 pKa = 4.71GLPDD370 pKa = 3.28NCMLMPP376 pKa = 5.43

Molecular weight: 39.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A120N0B8|A0A120N0B8_9CAUD Uncharacterized protein OS=Acaryochloris phage A-HIS1 OX=645116 GN=AHIS1_p094 PE=4 SV=1
MM1 pKa = 6.85EE2 pKa = 4.64TRR4 pKa = 11.84PKK6 pKa = 10.27RR7 pKa = 11.84SQPSGEE13 pKa = 4.27NPNNDD18 pKa = 3.07TKK20 pKa = 11.02TPASRR25 pKa = 11.84KK26 pKa = 8.57KK27 pKa = 10.12SGRR30 pKa = 11.84YY31 pKa = 8.97SYY33 pKa = 11.54ALTVVIEE40 pKa = 4.16RR41 pKa = 11.84TVEE44 pKa = 3.74YY45 pKa = 9.81IQRR48 pKa = 11.84SRR50 pKa = 11.84YY51 pKa = 8.69RR52 pKa = 3.28

Molecular weight: 6.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

17243

28

993

181.5

20.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.858 ± 0.457

1.015 ± 0.123

5.776 ± 0.198

7.099 ± 0.271

4.541 ± 0.184

5.985 ± 0.319

1.891 ± 0.194

6.623 ± 0.17

5.289 ± 0.397

8.601 ± 0.25

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.983 ± 0.166

5.422 ± 0.223

4.205 ± 0.221

2.465 ± 0.162

5.898 ± 0.366

6.635 ± 0.201

7.18 ± 0.335

6.588 ± 0.258

1.084 ± 0.094

3.862 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski