Amino acid dipepetide frequency for Mycobacterium phage MalagasyRose

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.181AlaAla: 19.181 ± 1.414
1.0AlaCys: 1.0 ± 0.319
8.061AlaAsp: 8.061 ± 0.783
7.06AlaGlu: 7.06 ± 0.871
3.118AlaPhe: 3.118 ± 0.576
10.238AlaGly: 10.238 ± 0.844
2.412AlaHis: 2.412 ± 0.357
4.589AlaIle: 4.589 ± 0.583
3.413AlaLys: 3.413 ± 0.411
12.12AlaLeu: 12.12 ± 0.779
2.648AlaMet: 2.648 ± 0.441
3.001AlaAsn: 3.001 ± 0.478
7.237AlaPro: 7.237 ± 0.917
4.472AlaGln: 4.472 ± 0.46
8.884AlaArg: 8.884 ± 0.879
4.766AlaSer: 4.766 ± 0.603
7.943AlaThr: 7.943 ± 0.705
9.12AlaVal: 9.12 ± 0.752
2.589AlaTrp: 2.589 ± 0.307
2.648AlaTyr: 2.648 ± 0.399
0.0AlaXaa: 0.0 ± 0.0
Cys
1.294CysAla: 1.294 ± 0.209
0.235CysCys: 0.235 ± 0.11
0.883CysAsp: 0.883 ± 0.25
0.765CysGlu: 0.765 ± 0.249
0.235CysPhe: 0.235 ± 0.109
1.942CysGly: 1.942 ± 0.381
0.294CysHis: 0.294 ± 0.133
0.471CysIle: 0.471 ± 0.142
0.235CysLys: 0.235 ± 0.108
0.353CysLeu: 0.353 ± 0.142
0.235CysMet: 0.235 ± 0.113
0.353CysAsn: 0.353 ± 0.116
0.588CysPro: 0.588 ± 0.178
0.177CysGln: 0.177 ± 0.105
1.118CysArg: 1.118 ± 0.292
0.353CysSer: 0.353 ± 0.147
0.353CysThr: 0.353 ± 0.128
0.824CysVal: 0.824 ± 0.249
0.177CysTrp: 0.177 ± 0.084
0.353CysTyr: 0.353 ± 0.133
0.0CysXaa: 0.0 ± 0.0
Asp
7.767AspAla: 7.767 ± 0.704
0.588AspCys: 0.588 ± 0.169
6.59AspAsp: 6.59 ± 0.83
4.884AspGlu: 4.884 ± 0.617
1.765AspPhe: 1.765 ± 0.337
6.296AspGly: 6.296 ± 0.478
1.647AspHis: 1.647 ± 0.327
0.941AspIle: 0.941 ± 0.199
1.236AspLys: 1.236 ± 0.241
6.001AspLeu: 6.001 ± 0.622
1.412AspMet: 1.412 ± 0.222
1.647AspAsn: 1.647 ± 0.295
4.766AspPro: 4.766 ± 0.471
1.883AspGln: 1.883 ± 0.285
4.236AspArg: 4.236 ± 0.515
2.648AspSer: 2.648 ± 0.425
3.942AspThr: 3.942 ± 0.469
4.942AspVal: 4.942 ± 0.53
1.765AspTrp: 1.765 ± 0.341
1.589AspTyr: 1.589 ± 0.32
0.0AspXaa: 0.0 ± 0.0
Glu
6.001GluAla: 6.001 ± 0.69
0.647GluCys: 0.647 ± 0.189
3.001GluAsp: 3.001 ± 0.372
2.471GluGlu: 2.471 ± 0.411
2.059GluPhe: 2.059 ± 0.35
4.177GluGly: 4.177 ± 0.488
1.412GluHis: 1.412 ± 0.303
2.118GluIle: 2.118 ± 0.34
1.294GluLys: 1.294 ± 0.32
4.884GluLeu: 4.884 ± 0.516
1.177GluMet: 1.177 ± 0.269
1.177GluAsn: 1.177 ± 0.238
3.413GluPro: 3.413 ± 0.567
2.648GluGln: 2.648 ± 0.418
4.766GluArg: 4.766 ± 0.582
3.001GluSer: 3.001 ± 0.373
2.353GluThr: 2.353 ± 0.536
5.295GluVal: 5.295 ± 0.554
0.824GluTrp: 0.824 ± 0.233
1.589GluTyr: 1.589 ± 0.29
0.0GluXaa: 0.0 ± 0.0
Phe
3.001PheAla: 3.001 ± 0.424
0.471PheCys: 0.471 ± 0.156
2.471PheAsp: 2.471 ± 0.276
1.53PheGlu: 1.53 ± 0.332
0.765PhePhe: 0.765 ± 0.191
3.177PheGly: 3.177 ± 0.426
0.53PheHis: 0.53 ± 0.195
0.941PheIle: 0.941 ± 0.206
0.706PheLys: 0.706 ± 0.215
1.765PheLeu: 1.765 ± 0.367
0.53PheMet: 0.53 ± 0.175
1.059PheAsn: 1.059 ± 0.236
0.765PhePro: 0.765 ± 0.248
0.353PheGln: 0.353 ± 0.183
2.059PheArg: 2.059 ± 0.319
1.236PheSer: 1.236 ± 0.29
2.648PheThr: 2.648 ± 0.41
1.706PheVal: 1.706 ± 0.316
0.412PheTrp: 0.412 ± 0.181
0.53PheTyr: 0.53 ± 0.159
0.0PheXaa: 0.0 ± 0.0
Gly
10.061GlyAla: 10.061 ± 1.027
1.471GlyCys: 1.471 ± 0.347
6.237GlyAsp: 6.237 ± 0.545
5.413GlyGlu: 5.413 ± 0.55
2.059GlyPhe: 2.059 ± 0.404
10.002GlyGly: 10.002 ± 1.533
2.353GlyHis: 2.353 ± 0.434
3.177GlyIle: 3.177 ± 0.481
3.883GlyLys: 3.883 ± 0.487
6.472GlyLeu: 6.472 ± 0.567
2.236GlyMet: 2.236 ± 0.376
2.824GlyAsn: 2.824 ± 0.521
3.942GlyPro: 3.942 ± 0.569
3.118GlyGln: 3.118 ± 0.613
4.119GlyArg: 4.119 ± 0.465
5.825GlySer: 5.825 ± 0.827
4.354GlyThr: 4.354 ± 0.713
7.237GlyVal: 7.237 ± 0.492
2.471GlyTrp: 2.471 ± 0.448
2.236GlyTyr: 2.236 ± 0.376
0.0GlyXaa: 0.0 ± 0.0
His
2.236HisAla: 2.236 ± 0.413
0.294HisCys: 0.294 ± 0.146
1.236HisAsp: 1.236 ± 0.264
1.353HisGlu: 1.353 ± 0.302
0.53HisPhe: 0.53 ± 0.184
1.883HisGly: 1.883 ± 0.368
0.824HisHis: 0.824 ± 0.219
0.765HisIle: 0.765 ± 0.211
0.824HisLys: 0.824 ± 0.211
2.118HisLeu: 2.118 ± 0.312
0.588HisMet: 0.588 ± 0.184
0.647HisAsn: 0.647 ± 0.191
1.471HisPro: 1.471 ± 0.324
1.0HisGln: 1.0 ± 0.196
1.706HisArg: 1.706 ± 0.359
0.941HisSer: 0.941 ± 0.224
1.53HisThr: 1.53 ± 0.398
2.295HisVal: 2.295 ± 0.344
0.706HisTrp: 0.706 ± 0.282
0.294HisTyr: 0.294 ± 0.158
0.0HisXaa: 0.0 ± 0.0
Ile
5.531IleAla: 5.531 ± 0.566
0.177IleCys: 0.177 ± 0.114
2.471IleAsp: 2.471 ± 0.389
2.707IleGlu: 2.707 ± 0.386
0.53IlePhe: 0.53 ± 0.194
3.413IleGly: 3.413 ± 0.54
0.412IleHis: 0.412 ± 0.17
0.883IleIle: 0.883 ± 0.225
1.0IleLys: 1.0 ± 0.259
2.648IleLeu: 2.648 ± 0.436
0.53IleMet: 0.53 ± 0.185
1.118IleAsn: 1.118 ± 0.305
2.648IlePro: 2.648 ± 0.484
1.0IleGln: 1.0 ± 0.248
1.942IleArg: 1.942 ± 0.335
2.0IleSer: 2.0 ± 0.355
3.001IleThr: 3.001 ± 0.46
2.883IleVal: 2.883 ± 0.397
0.294IleTrp: 0.294 ± 0.113
0.471IleTyr: 0.471 ± 0.178
0.0IleXaa: 0.0 ± 0.0
Lys
3.354LysAla: 3.354 ± 0.417
0.177LysCys: 0.177 ± 0.133
0.941LysAsp: 0.941 ± 0.231
0.588LysGlu: 0.588 ± 0.183
1.177LysPhe: 1.177 ± 0.25
1.706LysGly: 1.706 ± 0.249
0.588LysHis: 0.588 ± 0.181
0.824LysIle: 0.824 ± 0.196
0.53LysLys: 0.53 ± 0.169
3.177LysLeu: 3.177 ± 0.4
0.53LysMet: 0.53 ± 0.19
0.588LysAsn: 0.588 ± 0.157
2.118LysPro: 2.118 ± 0.471
1.294LysGln: 1.294 ± 0.274
3.001LysArg: 3.001 ± 0.362
2.177LysSer: 2.177 ± 0.352
1.589LysThr: 1.589 ± 0.231
2.471LysVal: 2.471 ± 0.321
0.647LysTrp: 0.647 ± 0.177
0.706LysTyr: 0.706 ± 0.216
0.0LysXaa: 0.0 ± 0.0
Leu
10.944LeuAla: 10.944 ± 0.765
0.765LeuCys: 0.765 ± 0.207
5.295LeuAsp: 5.295 ± 0.561
4.295LeuGlu: 4.295 ± 0.394
2.177LeuPhe: 2.177 ± 0.409
6.766LeuGly: 6.766 ± 0.774
2.118LeuHis: 2.118 ± 0.477
3.06LeuIle: 3.06 ± 0.442
2.589LeuLys: 2.589 ± 0.321
6.237LeuLeu: 6.237 ± 0.495
1.53LeuMet: 1.53 ± 0.322
2.765LeuAsn: 2.765 ± 0.317
4.825LeuPro: 4.825 ± 0.506
3.295LeuGln: 3.295 ± 0.431
7.649LeuArg: 7.649 ± 0.737
4.413LeuSer: 4.413 ± 0.527
5.413LeuThr: 5.413 ± 0.488
6.178LeuVal: 6.178 ± 0.528
1.589LeuTrp: 1.589 ± 0.376
2.118LeuTyr: 2.118 ± 0.381
0.0LeuXaa: 0.0 ± 0.0
Met
2.236MetAla: 2.236 ± 0.316
0.353MetCys: 0.353 ± 0.202
0.471MetAsp: 0.471 ± 0.143
0.588MetGlu: 0.588 ± 0.206
0.824MetPhe: 0.824 ± 0.204
1.883MetGly: 1.883 ± 0.321
0.471MetHis: 0.471 ± 0.17
0.471MetIle: 0.471 ± 0.134
0.235MetLys: 0.235 ± 0.108
2.295MetLeu: 2.295 ± 0.411
0.471MetMet: 0.471 ± 0.158
0.647MetAsn: 0.647 ± 0.222
2.059MetPro: 2.059 ± 0.45
0.353MetGln: 0.353 ± 0.13
1.824MetArg: 1.824 ± 0.326
1.706MetSer: 1.706 ± 0.309
1.824MetThr: 1.824 ± 0.365
1.236MetVal: 1.236 ± 0.373
0.235MetTrp: 0.235 ± 0.121
0.412MetTyr: 0.412 ± 0.158
0.0MetXaa: 0.0 ± 0.0
Asn
3.06AsnAla: 3.06 ± 0.385
0.353AsnCys: 0.353 ± 0.133
2.059AsnAsp: 2.059 ± 0.35
0.765AsnGlu: 0.765 ± 0.192
0.941AsnPhe: 0.941 ± 0.224
3.648AsnGly: 3.648 ± 0.492
0.53AsnHis: 0.53 ± 0.173
1.294AsnIle: 1.294 ± 0.253
0.706AsnLys: 0.706 ± 0.19
2.353AsnLeu: 2.353 ± 0.305
0.53AsnMet: 0.53 ± 0.201
0.53AsnAsn: 0.53 ± 0.164
1.883AsnPro: 1.883 ± 0.316
0.471AsnGln: 0.471 ± 0.179
2.295AsnArg: 2.295 ± 0.401
1.236AsnSer: 1.236 ± 0.315
1.471AsnThr: 1.471 ± 0.262
2.236AsnVal: 2.236 ± 0.375
0.412AsnTrp: 0.412 ± 0.136
0.647AsnTyr: 0.647 ± 0.194
0.0AsnXaa: 0.0 ± 0.0
Pro
7.237ProAla: 7.237 ± 1.117
0.353ProCys: 0.353 ± 0.125
4.295ProAsp: 4.295 ± 0.581
3.707ProGlu: 3.707 ± 0.447
1.118ProPhe: 1.118 ± 0.276
6.766ProGly: 6.766 ± 0.723
1.059ProHis: 1.059 ± 0.219
2.295ProIle: 2.295 ± 0.343
2.0ProLys: 2.0 ± 0.288
4.295ProLeu: 4.295 ± 0.484
0.883ProMet: 0.883 ± 0.253
1.942ProAsn: 1.942 ± 0.426
5.001ProPro: 5.001 ± 0.737
1.765ProGln: 1.765 ± 0.349
4.177ProArg: 4.177 ± 0.49
2.707ProSer: 2.707 ± 0.416
5.295ProThr: 5.295 ± 0.656
5.413ProVal: 5.413 ± 0.55
1.059ProTrp: 1.059 ± 0.262
1.471ProTyr: 1.471 ± 0.355
0.0ProXaa: 0.0 ± 0.0
Gln
4.413GlnAla: 4.413 ± 0.51
0.471GlnCys: 0.471 ± 0.15
1.412GlnAsp: 1.412 ± 0.322
1.589GlnGlu: 1.589 ± 0.331
0.883GlnPhe: 0.883 ± 0.191
2.353GlnGly: 2.353 ± 0.377
0.883GlnHis: 0.883 ± 0.214
2.059GlnIle: 2.059 ± 0.34
0.941GlnLys: 0.941 ± 0.219
3.53GlnLeu: 3.53 ± 0.461
0.824GlnMet: 0.824 ± 0.217
0.353GlnAsn: 0.353 ± 0.15
2.236GlnPro: 2.236 ± 0.277
1.589GlnGln: 1.589 ± 0.316
3.354GlnArg: 3.354 ± 0.5
1.883GlnSer: 1.883 ± 0.362
1.706GlnThr: 1.706 ± 0.311
2.883GlnVal: 2.883 ± 0.401
0.883GlnTrp: 0.883 ± 0.251
0.883GlnTyr: 0.883 ± 0.256
0.0GlnXaa: 0.0 ± 0.0
Arg
8.296ArgAla: 8.296 ± 0.857
0.883ArgCys: 0.883 ± 0.251
4.825ArgAsp: 4.825 ± 0.651
5.119ArgGlu: 5.119 ± 0.666
2.295ArgPhe: 2.295 ± 0.395
4.884ArgGly: 4.884 ± 0.488
2.0ArgHis: 2.0 ± 0.411
3.177ArgIle: 3.177 ± 0.443
2.295ArgLys: 2.295 ± 0.461
6.237ArgLeu: 6.237 ± 0.551
1.412ArgMet: 1.412 ± 0.26
2.0ArgAsn: 2.0 ± 0.384
4.707ArgPro: 4.707 ± 0.634
2.059ArgGln: 2.059 ± 0.342
5.472ArgArg: 5.472 ± 0.646
2.824ArgSer: 2.824 ± 0.405
3.883ArgThr: 3.883 ± 0.566
5.472ArgVal: 5.472 ± 0.58
1.824ArgTrp: 1.824 ± 0.36
2.236ArgTyr: 2.236 ± 0.386
0.0ArgXaa: 0.0 ± 0.0
Ser
6.237SerAla: 6.237 ± 0.797
0.471SerCys: 0.471 ± 0.198
3.471SerAsp: 3.471 ± 0.481
2.353SerGlu: 2.353 ± 0.408
1.294SerPhe: 1.294 ± 0.273
4.472SerGly: 4.472 ± 0.66
1.236SerHis: 1.236 ± 0.282
1.53SerIle: 1.53 ± 0.401
1.589SerLys: 1.589 ± 0.305
3.53SerLeu: 3.53 ± 0.534
1.353SerMet: 1.353 ± 0.29
1.883SerAsn: 1.883 ± 0.334
3.53SerPro: 3.53 ± 0.403
2.353SerGln: 2.353 ± 0.416
2.53SerArg: 2.53 ± 0.36
2.177SerSer: 2.177 ± 0.531
2.648SerThr: 2.648 ± 0.418
3.707SerVal: 3.707 ± 0.536
1.118SerTrp: 1.118 ± 0.233
1.53SerTyr: 1.53 ± 0.234
0.0SerXaa: 0.0 ± 0.0
Thr
7.943ThrAla: 7.943 ± 0.687
0.765ThrCys: 0.765 ± 0.174
4.06ThrAsp: 4.06 ± 0.462
3.06ThrGlu: 3.06 ± 0.372
1.412ThrPhe: 1.412 ± 0.239
6.001ThrGly: 6.001 ± 0.755
0.883ThrHis: 0.883 ± 0.219
2.824ThrIle: 2.824 ± 0.414
1.353ThrLys: 1.353 ± 0.24
4.825ThrLeu: 4.825 ± 0.607
1.177ThrMet: 1.177 ± 0.224
1.942ThrAsn: 1.942 ± 0.316
4.354ThrPro: 4.354 ± 0.677
2.0ThrGln: 2.0 ± 0.334
3.766ThrArg: 3.766 ± 0.435
2.353ThrSer: 2.353 ± 0.42
3.589ThrThr: 3.589 ± 0.469
5.766ThrVal: 5.766 ± 0.631
2.059ThrTrp: 2.059 ± 0.317
1.883ThrTyr: 1.883 ± 0.349
0.0ThrXaa: 0.0 ± 0.0
Val
10.826ValAla: 10.826 ± 0.829
1.177ValCys: 1.177 ± 0.291
6.06ValAsp: 6.06 ± 0.629
3.942ValGlu: 3.942 ± 0.56
2.177ValPhe: 2.177 ± 0.343
6.001ValGly: 6.001 ± 0.639
2.059ValHis: 2.059 ± 0.332
2.53ValIle: 2.53 ± 0.488
2.295ValLys: 2.295 ± 0.458
6.119ValLeu: 6.119 ± 0.492
1.765ValMet: 1.765 ± 0.332
1.942ValAsn: 1.942 ± 0.339
4.884ValPro: 4.884 ± 0.558
3.413ValGln: 3.413 ± 0.494
5.237ValArg: 5.237 ± 0.506
4.001ValSer: 4.001 ± 0.54
5.707ValThr: 5.707 ± 0.733
6.884ValVal: 6.884 ± 0.827
1.942ValTrp: 1.942 ± 0.259
1.706ValTyr: 1.706 ± 0.276
0.0ValXaa: 0.0 ± 0.0
Trp
2.353TrpAla: 2.353 ± 0.349
0.353TrpCys: 0.353 ± 0.142
1.0TrpAsp: 1.0 ± 0.223
1.0TrpGlu: 1.0 ± 0.29
0.647TrpPhe: 0.647 ± 0.187
1.647TrpGly: 1.647 ± 0.322
0.941TrpHis: 0.941 ± 0.246
0.765TrpIle: 0.765 ± 0.203
0.471TrpLys: 0.471 ± 0.207
2.824TrpLeu: 2.824 ± 0.432
0.177TrpMet: 0.177 ± 0.085
0.353TrpAsn: 0.353 ± 0.14
1.118TrpPro: 1.118 ± 0.236
1.236TrpGln: 1.236 ± 0.244
1.412TrpArg: 1.412 ± 0.299
1.471TrpSer: 1.471 ± 0.362
1.177TrpThr: 1.177 ± 0.275
1.942TrpVal: 1.942 ± 0.387
0.588TrpTrp: 0.588 ± 0.209
0.588TrpTyr: 0.588 ± 0.208
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.53TyrAla: 2.53 ± 0.349
0.235TyrCys: 0.235 ± 0.137
1.706TyrAsp: 1.706 ± 0.439
1.118TyrGlu: 1.118 ± 0.265
0.647TyrPhe: 0.647 ± 0.191
2.236TyrGly: 2.236 ± 0.364
0.588TyrHis: 0.588 ± 0.221
0.941TyrIle: 0.941 ± 0.3
0.588TyrLys: 0.588 ± 0.169
2.295TyrLeu: 2.295 ± 0.319
0.53TyrMet: 0.53 ± 0.149
0.647TyrAsn: 0.647 ± 0.208
1.294TyrPro: 1.294 ± 0.316
0.647TyrGln: 0.647 ± 0.196
2.353TyrArg: 2.353 ± 0.351
1.294TyrSer: 1.294 ± 0.25
1.589TyrThr: 1.589 ± 0.321
2.177TyrVal: 2.177 ± 0.378
0.471TyrTrp: 0.471 ± 0.155
0.647TyrTyr: 0.647 ± 0.216
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 84 proteins (16997 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski