Botrytis porri

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Sclerotiniaceae; Botrytis

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11857 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Z1KGE8|A0A4Z1KGE8_9HELO N-acetylglucosamine-6-phosphate deacetylase OS=Botrytis porri OX=87229 GN=BPOR_0669g00030 PE=3 SV=1
MM1 pKa = 7.55NSWQDD6 pKa = 3.01EE7 pKa = 4.42TQRR10 pKa = 11.84WRR12 pKa = 11.84TEE14 pKa = 3.57HH15 pKa = 6.96LLNANIKK22 pKa = 9.66NEE24 pKa = 4.14RR25 pKa = 11.84PWKK28 pKa = 8.96VLSRR32 pKa = 11.84QAYY35 pKa = 8.32WEE37 pKa = 4.46LLDD40 pKa = 6.11DD41 pKa = 4.97SDD43 pKa = 4.92SEE45 pKa = 4.26YY46 pKa = 10.95DD47 pKa = 4.03ISDD50 pKa = 3.57EE51 pKa = 4.72HH52 pKa = 7.71LGHH55 pKa = 6.83YY56 pKa = 9.15DD57 pKa = 3.74GCIDD61 pKa = 5.64CYY63 pKa = 11.15DD64 pKa = 4.37CEE66 pKa = 4.85DD67 pKa = 4.12DD68 pKa = 5.76EE69 pKa = 4.56EE70 pKa = 5.75VEE72 pKa = 5.45DD73 pKa = 4.07EE74 pKa = 5.25DD75 pKa = 4.79EE76 pKa = 4.52VGDD79 pKa = 3.92YY80 pKa = 11.45VEE82 pKa = 5.19DD83 pKa = 4.72EE84 pKa = 4.39EE85 pKa = 6.17MSDD88 pKa = 3.85QNN90 pKa = 4.19

Molecular weight: 10.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Z1KMM7|A0A4Z1KMM7_9HELO Uncharacterized protein OS=Botrytis porri OX=87229 GN=BPOR_0384g00110 PE=4 SV=1
MM1 pKa = 7.94PSQKK5 pKa = 10.41SFRR8 pKa = 11.84TKK10 pKa = 10.33QKK12 pKa = 10.05LARR15 pKa = 11.84AQKK18 pKa = 8.97QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.15WRR45 pKa = 11.84KK46 pKa = 7.46TRR48 pKa = 11.84IGLL51 pKa = 3.73

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11857

0

11857

5343889

23

6825

450.7

50.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.679 ± 0.019

1.168 ± 0.007

5.558 ± 0.016

6.612 ± 0.03

3.706 ± 0.015

6.729 ± 0.026

2.284 ± 0.011

5.476 ± 0.015

5.492 ± 0.022

8.49 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.245 ± 0.01

4.262 ± 0.014

5.8 ± 0.023

3.89 ± 0.016

5.714 ± 0.023

8.823 ± 0.034

6.169 ± 0.019

5.739 ± 0.015

1.357 ± 0.009

2.806 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski