Smittium culicis

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Zoopagomycota; Kickxellomycotina; Harpellomycetes; Harpellales; Legeriomycetaceae; Smittium

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10832 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R1XZG2|A0A1R1XZG2_9FUNG Uncharacterized protein OS=Smittium culicis OX=133412 GN=AYI70_g4405 PE=4 SV=1
MM1 pKa = 7.4SVNSSNPSSPYY12 pKa = 10.06KK13 pKa = 10.8EE14 pKa = 4.24NFEE17 pKa = 4.45SANPNYY23 pKa = 9.93HH24 pKa = 7.43DD25 pKa = 4.29YY26 pKa = 10.97FGSSDD31 pKa = 3.16VDD33 pKa = 3.85AEE35 pKa = 4.34VSGLDD40 pKa = 3.32SHH42 pKa = 7.45FSLDD46 pKa = 3.16IDD48 pKa = 3.69NDD50 pKa = 3.8EE51 pKa = 4.76LSSEE55 pKa = 4.15IHH57 pKa = 5.77QSTNITTGNSTDD69 pKa = 4.57NIDD72 pKa = 4.09EE73 pKa = 4.69ANLLDD78 pKa = 4.56WIDD81 pKa = 3.59KK82 pKa = 10.12FFAKK86 pKa = 10.34KK87 pKa = 10.23NNQSEE92 pKa = 4.22NVYY95 pKa = 10.45QSNIDD100 pKa = 3.43FRR102 pKa = 11.84LVLKK106 pKa = 10.37YY107 pKa = 10.5QLDD110 pKa = 3.96LEE112 pKa = 4.55RR113 pKa = 11.84DD114 pKa = 3.42QLLTTKK120 pKa = 10.58NYY122 pKa = 9.18FQSQLEE128 pKa = 4.27VLSNDD133 pKa = 3.04QKK135 pKa = 10.9RR136 pKa = 11.84YY137 pKa = 10.04RR138 pKa = 11.84SILGYY143 pKa = 9.01TPDD146 pKa = 3.37VSDD149 pKa = 3.99YY150 pKa = 11.5SSDD153 pKa = 4.52SEE155 pKa = 5.42DD156 pKa = 3.35HH157 pKa = 6.19LQPSKK162 pKa = 11.63SNDD165 pKa = 3.26APNLSSPVYY174 pKa = 9.89DD175 pKa = 3.67TFQLSEE181 pKa = 4.7DD182 pKa = 3.87YY183 pKa = 10.84ANPMDD188 pKa = 4.57YY189 pKa = 10.48FDD191 pKa = 5.18KK192 pKa = 10.89NSPEE196 pKa = 4.27IGQSSPVDD204 pKa = 3.6YY205 pKa = 10.83QSSSEE210 pKa = 4.11NYY212 pKa = 9.6YY213 pKa = 10.79SAIDD217 pKa = 3.67SGSEE221 pKa = 3.9SDD223 pKa = 4.09SGEE226 pKa = 3.96VDD228 pKa = 3.19PDD230 pKa = 3.34QALQSLKK237 pKa = 10.95ALLSQYY243 pKa = 11.28DD244 pKa = 3.62NPP246 pKa = 4.4

Molecular weight: 27.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R1Y025|A0A1R1Y025_9FUNG Uncharacterized protein OS=Smittium culicis OX=133412 GN=AYI70_g4245 PE=4 SV=1
MM1 pKa = 7.33NVLKK5 pKa = 10.52LVWRR9 pKa = 11.84NTRR12 pKa = 11.84RR13 pKa = 11.84NTRR16 pKa = 11.84RR17 pKa = 11.84NTRR20 pKa = 11.84RR21 pKa = 11.84NTRR24 pKa = 11.84KK25 pKa = 9.97SISHH29 pKa = 5.79ITSHH33 pKa = 5.33TTNHH37 pKa = 5.22TTNHH41 pKa = 4.98TTSHH45 pKa = 5.18TTSHH49 pKa = 5.5TTSHH53 pKa = 5.5TTSHH57 pKa = 5.5TTSHH61 pKa = 5.44TTSHH65 pKa = 5.75STSHH69 pKa = 5.66STSHH73 pKa = 5.67STSHH77 pKa = 5.67STSHH81 pKa = 5.67STSHH85 pKa = 5.7STSHH89 pKa = 5.34TTSHH93 pKa = 5.8STSHH97 pKa = 5.08TTSHH101 pKa = 5.93KK102 pKa = 8.29TSHH105 pKa = 5.77STSHH109 pKa = 5.65STSHH113 pKa = 5.67STSHH117 pKa = 5.67STSHH121 pKa = 5.7STSHH125 pKa = 5.3TTSHH129 pKa = 6.36SSSHH133 pKa = 4.6TTSHH137 pKa = 5.66KK138 pKa = 9.04TSHH141 pKa = 5.76KK142 pKa = 8.09TSHH145 pKa = 5.75KK146 pKa = 8.09TSHH149 pKa = 5.75KK150 pKa = 8.09TSHH153 pKa = 5.75KK154 pKa = 8.48TSHH157 pKa = 5.85KK158 pKa = 9.45TRR160 pKa = 11.84FTRR163 pKa = 11.84TARR166 pKa = 11.84TTITITANLL175 pKa = 3.68

Molecular weight: 19.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10832

0

10832

4382278

8

7231

404.6

45.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.29 ± 0.021

1.175 ± 0.009

5.758 ± 0.018

5.719 ± 0.025

4.436 ± 0.014

4.663 ± 0.025

1.856 ± 0.01

7.219 ± 0.019

7.477 ± 0.028

8.702 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.807 ± 0.01

7.544 ± 0.035

4.677 ± 0.022

3.556 ± 0.023

3.919 ± 0.021

11.506 ± 0.04

5.473 ± 0.024

5.053 ± 0.018

0.765 ± 0.007

3.388 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski