Pseudogymnoascus sp. VKM F-4518 (FW-2643)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus; unclassified Pseudogymnoascus

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11618 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A094HP85|A0A094HP85_9PEZI Uncharacterized protein OS=Pseudogymnoascus sp. VKM F-4518 (FW-2643) OX=1420913 GN=V500_07927 PE=4 SV=1
MM1 pKa = 7.05VAIKK5 pKa = 10.14PVFALSLLAGLITPALSAAVKK26 pKa = 10.17INAFGDD32 pKa = 4.24SITGSPGCWRR42 pKa = 11.84ALLYY46 pKa = 10.54QKK48 pKa = 10.54LVEE51 pKa = 5.28AGTTDD56 pKa = 2.88IDD58 pKa = 4.5FVGTLPGQGCGIEE71 pKa = 3.98YY72 pKa = 10.6DD73 pKa = 4.23GEE75 pKa = 4.34NDD77 pKa = 3.27GHH79 pKa = 7.39GGFLATGIVADD90 pKa = 3.97NQLPGWLAISQPDD103 pKa = 3.44IVMMQLATNDD113 pKa = 3.04VWSNIATATILDD125 pKa = 4.08AFSTLVDD132 pKa = 3.18QMRR135 pKa = 11.84DD136 pKa = 3.31SKK138 pKa = 10.74STMHH142 pKa = 6.54IVVAQITPMDD152 pKa = 4.24PTDD155 pKa = 3.45GCATCEE161 pKa = 3.88AGIIALNDD169 pKa = 5.18AIPAWAAEE177 pKa = 4.04KK178 pKa = 9.9STIEE182 pKa = 4.06SPITVVDD189 pKa = 4.99CYY191 pKa = 10.57TGYY194 pKa = 8.59DD195 pKa = 3.85TATDD199 pKa = 3.96TYY201 pKa = 11.53DD202 pKa = 3.5GVHH205 pKa = 6.79PNDD208 pKa = 3.71SGNVKK213 pKa = 10.25LADD216 pKa = 3.21AWFEE220 pKa = 3.91PLQAAILAASSGTNSTIAA238 pKa = 3.51

Molecular weight: 24.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A094H2U1|A0A094H2U1_9PEZI Uncharacterized protein (Fragment) OS=Pseudogymnoascus sp. VKM F-4518 (FW-2643) OX=1420913 GN=V500_00024 PE=4 SV=1
MM1 pKa = 7.49SKK3 pKa = 10.64YY4 pKa = 10.79NKK6 pKa = 9.67LFKK9 pKa = 10.32PRR11 pKa = 11.84LQPFNLFSGDD21 pKa = 3.52TGLSTLLPPNSITPHH36 pKa = 6.27GISYY40 pKa = 7.56TQHH43 pKa = 5.33PHH45 pKa = 4.86QRR47 pKa = 11.84QPNNQHH53 pKa = 6.22PAHH56 pKa = 6.37RR57 pKa = 11.84QAPRR61 pKa = 11.84RR62 pKa = 11.84PRR64 pKa = 11.84TAKK67 pKa = 9.72VPRR70 pKa = 11.84FIALPRR76 pKa = 11.84APPVNIRR83 pKa = 11.84FHH85 pKa = 5.69QALVHH90 pKa = 6.65DD91 pKa = 4.62LLGRR95 pKa = 11.84EE96 pKa = 4.08IGSLAVLEE104 pKa = 4.41ARR106 pKa = 11.84SIIAVDD112 pKa = 3.81LGFGVSAARR121 pKa = 11.84LEE123 pKa = 4.21GLVVAGCGGVEE134 pKa = 3.83GRR136 pKa = 11.84EE137 pKa = 3.95AGGFAGRR144 pKa = 11.84VDD146 pKa = 3.1MWEE149 pKa = 3.96LVEE152 pKa = 4.37AA153 pKa = 4.75

Molecular weight: 16.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11618

0

11618

5826161

21

5625

501.5

55.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.926 ± 0.023

1.22 ± 0.008

5.646 ± 0.015

6.276 ± 0.024

3.632 ± 0.014

7.24 ± 0.021

2.259 ± 0.009

5.111 ± 0.015

4.908 ± 0.022

8.804 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.127 ± 0.008

3.738 ± 0.011

5.889 ± 0.022

3.854 ± 0.016

5.843 ± 0.019

8.077 ± 0.023

6.065 ± 0.016

6.182 ± 0.015

1.434 ± 0.007

2.77 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski