Noviherbaspirillum humi

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Oxalobacteraceae; Noviherbaspirillum

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4789 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A239LIH2|A0A239LIH2_9BURK Transcriptional regulator GntR family OS=Noviherbaspirillum humi OX=1688639 GN=SAMN06265795_12326 PE=4 SV=1
MM1 pKa = 7.15NAVTEE6 pKa = 4.35MPAPIVFTDD15 pKa = 3.78SAAMKK20 pKa = 9.71VAEE23 pKa = 5.17LIAEE27 pKa = 4.35EE28 pKa = 4.62GNPDD32 pKa = 2.99LKK34 pKa = 11.13LRR36 pKa = 11.84VFVQGGGCSGFQYY49 pKa = 10.94GFTFDD54 pKa = 4.69EE55 pKa = 4.93IVNEE59 pKa = 4.96DD60 pKa = 3.85DD61 pKa = 3.19TTMEE65 pKa = 4.19KK66 pKa = 10.82NGVQLLIDD74 pKa = 3.77SMSYY78 pKa = 10.23QYY80 pKa = 11.55LVGAEE85 pKa = 4.01IDD87 pKa = 3.89YY88 pKa = 11.28KK89 pKa = 11.39DD90 pKa = 4.47DD91 pKa = 4.08LEE93 pKa = 4.56GAQFVIKK100 pKa = 10.5NPNATTTCGCGSSFSVV116 pKa = 3.54

Molecular weight: 12.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A239C7T0|A0A239C7T0_9BURK DNA topoisomerase OS=Noviherbaspirillum humi OX=1688639 GN=SAMN06265795_101311 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 9.97QPSVVRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.57RR14 pKa = 11.84THH16 pKa = 5.79GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84SGRR28 pKa = 11.84AILNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.68GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4789

0

4789

1549550

25

3650

323.6

35.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.233 ± 0.048

0.908 ± 0.014

5.277 ± 0.029

5.535 ± 0.041

3.616 ± 0.023

8.156 ± 0.044

2.171 ± 0.021

4.976 ± 0.023

3.696 ± 0.036

10.36 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.637 ± 0.023

3.062 ± 0.035

5.137 ± 0.032

3.981 ± 0.024

6.805 ± 0.042

5.717 ± 0.04

4.955 ± 0.042

7.111 ± 0.03

1.284 ± 0.015

2.383 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski