Amino acid dipepetide frequency for Gordonia phage Hello

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.846AlaAla: 13.846 ± 4.125
0.619AlaCys: 0.619 ± 0.176
4.91AlaAsp: 4.91 ± 0.43
7.255AlaGlu: 7.255 ± 0.735
2.875AlaPhe: 2.875 ± 0.314
7.343AlaGly: 7.343 ± 0.868
1.194AlaHis: 1.194 ± 0.249
5.308AlaIle: 5.308 ± 0.751
4.158AlaLys: 4.158 ± 0.477
8.228AlaLeu: 8.228 ± 0.968
3.052AlaMet: 3.052 ± 0.401
3.052AlaAsn: 3.052 ± 0.438
4.556AlaPro: 4.556 ± 0.458
3.937AlaGln: 3.937 ± 0.631
5.706AlaArg: 5.706 ± 0.552
5.751AlaSer: 5.751 ± 0.727
6.016AlaThr: 6.016 ± 0.504
6.857AlaVal: 6.857 ± 0.863
1.902AlaTrp: 1.902 ± 0.54
2.566AlaTyr: 2.566 ± 0.267
0.0AlaXaa: 0.0 ± 0.0
Cys
0.752CysAla: 0.752 ± 0.199
0.133CysCys: 0.133 ± 0.083
0.619CysAsp: 0.619 ± 0.205
0.398CysGlu: 0.398 ± 0.124
0.354CysPhe: 0.354 ± 0.13
0.973CysGly: 0.973 ± 0.275
0.354CysHis: 0.354 ± 0.164
0.575CysIle: 0.575 ± 0.185
0.398CysLys: 0.398 ± 0.133
0.265CysLeu: 0.265 ± 0.116
0.221CysMet: 0.221 ± 0.097
0.354CysAsn: 0.354 ± 0.112
0.575CysPro: 0.575 ± 0.174
0.265CysGln: 0.265 ± 0.098
0.664CysArg: 0.664 ± 0.192
0.796CysSer: 0.796 ± 0.191
0.619CysThr: 0.619 ± 0.176
0.664CysVal: 0.664 ± 0.193
0.133CysTrp: 0.133 ± 0.073
0.31CysTyr: 0.31 ± 0.115
0.0CysXaa: 0.0 ± 0.0
Asp
5.264AspAla: 5.264 ± 0.517
0.531AspCys: 0.531 ± 0.16
4.335AspAsp: 4.335 ± 0.731
4.999AspGlu: 4.999 ± 0.601
2.345AspPhe: 2.345 ± 0.279
4.689AspGly: 4.689 ± 0.569
1.017AspHis: 1.017 ± 0.202
3.406AspIle: 3.406 ± 0.333
2.831AspLys: 2.831 ± 0.374
4.291AspLeu: 4.291 ± 0.451
1.46AspMet: 1.46 ± 0.235
2.92AspAsn: 2.92 ± 0.376
3.849AspPro: 3.849 ± 0.588
2.035AspGln: 2.035 ± 0.256
2.61AspArg: 2.61 ± 0.403
3.45AspSer: 3.45 ± 0.336
3.45AspThr: 3.45 ± 0.405
4.247AspVal: 4.247 ± 0.572
1.283AspTrp: 1.283 ± 0.237
2.168AspTyr: 2.168 ± 0.415
0.0AspXaa: 0.0 ± 0.0
Glu
6.547GluAla: 6.547 ± 0.606
0.973GluCys: 0.973 ± 0.248
3.981GluAsp: 3.981 ± 0.438
6.06GluGlu: 6.06 ± 0.632
2.433GluPhe: 2.433 ± 0.357
4.866GluGly: 4.866 ± 0.458
0.973GluHis: 0.973 ± 0.207
3.893GluIle: 3.893 ± 0.333
2.831GluLys: 2.831 ± 0.436
6.06GluLeu: 6.06 ± 0.806
1.548GluMet: 1.548 ± 0.21
1.769GluAsn: 1.769 ± 0.241
2.654GluPro: 2.654 ± 0.52
3.185GluGln: 3.185 ± 0.427
4.954GluArg: 4.954 ± 0.726
4.158GluSer: 4.158 ± 0.389
3.893GluThr: 3.893 ± 0.488
5.176GluVal: 5.176 ± 0.41
1.239GluTrp: 1.239 ± 0.262
2.521GluTyr: 2.521 ± 0.397
0.0GluXaa: 0.0 ± 0.0
Phe
3.45PheAla: 3.45 ± 0.32
0.398PheCys: 0.398 ± 0.128
2.964PheAsp: 2.964 ± 0.389
2.743PheGlu: 2.743 ± 0.367
1.017PhePhe: 1.017 ± 0.218
3.406PheGly: 3.406 ± 0.417
0.752PheHis: 0.752 ± 0.222
1.991PheIle: 1.991 ± 0.297
2.168PheLys: 2.168 ± 0.252
1.858PheLeu: 1.858 ± 0.261
1.017PheMet: 1.017 ± 0.195
1.637PheAsn: 1.637 ± 0.232
1.46PhePro: 1.46 ± 0.263
1.062PheGln: 1.062 ± 0.155
1.592PheArg: 1.592 ± 0.303
2.566PheSer: 2.566 ± 0.359
2.168PheThr: 2.168 ± 0.326
1.946PheVal: 1.946 ± 0.317
0.354PheTrp: 0.354 ± 0.09
0.973PheTyr: 0.973 ± 0.169
0.0PheXaa: 0.0 ± 0.0
Gly
6.724GlyAla: 6.724 ± 0.919
0.84GlyCys: 0.84 ± 0.205
4.733GlyAsp: 4.733 ± 0.446
4.645GlyGlu: 4.645 ± 0.429
2.831GlyPhe: 2.831 ± 0.348
8.139GlyGly: 8.139 ± 0.971
1.15GlyHis: 1.15 ± 0.272
4.114GlyIle: 4.114 ± 0.537
4.954GlyLys: 4.954 ± 0.463
5.176GlyLeu: 5.176 ± 0.619
1.769GlyMet: 1.769 ± 0.264
3.583GlyAsn: 3.583 ± 0.43
3.672GlyPro: 3.672 ± 0.382
2.256GlyGln: 2.256 ± 0.311
4.07GlyArg: 4.07 ± 0.551
5.53GlySer: 5.53 ± 0.575
5.662GlyThr: 5.662 ± 0.663
5.706GlyVal: 5.706 ± 0.451
1.637GlyTrp: 1.637 ± 0.266
2.61GlyTyr: 2.61 ± 0.344
0.0GlyXaa: 0.0 ± 0.0
His
1.327HisAla: 1.327 ± 0.263
0.31HisCys: 0.31 ± 0.125
0.796HisAsp: 0.796 ± 0.24
0.929HisGlu: 0.929 ± 0.226
0.398HisPhe: 0.398 ± 0.125
1.15HisGly: 1.15 ± 0.223
0.31HisHis: 0.31 ± 0.122
1.017HisIle: 1.017 ± 0.211
0.929HisLys: 0.929 ± 0.233
1.592HisLeu: 1.592 ± 0.243
0.442HisMet: 0.442 ± 0.15
0.619HisAsn: 0.619 ± 0.139
1.194HisPro: 1.194 ± 0.273
0.752HisGln: 0.752 ± 0.218
1.106HisArg: 1.106 ± 0.279
1.062HisSer: 1.062 ± 0.211
0.752HisThr: 0.752 ± 0.167
0.973HisVal: 0.973 ± 0.205
0.265HisTrp: 0.265 ± 0.11
0.575HisTyr: 0.575 ± 0.159
0.0HisXaa: 0.0 ± 0.0
Ile
5.353IleAla: 5.353 ± 0.798
0.31IleCys: 0.31 ± 0.114
4.158IleAsp: 4.158 ± 0.443
3.937IleGlu: 3.937 ± 0.469
1.858IlePhe: 1.858 ± 0.301
4.91IleGly: 4.91 ± 0.586
0.708IleHis: 0.708 ± 0.182
3.362IleIle: 3.362 ± 0.375
3.141IleLys: 3.141 ± 0.41
3.185IleLeu: 3.185 ± 0.33
0.885IleMet: 0.885 ± 0.171
2.123IleAsn: 2.123 ± 0.257
2.566IlePro: 2.566 ± 0.325
2.831IleGln: 2.831 ± 0.343
3.185IleArg: 3.185 ± 0.407
2.787IleSer: 2.787 ± 0.255
2.831IleThr: 2.831 ± 0.326
3.539IleVal: 3.539 ± 0.354
0.929IleTrp: 0.929 ± 0.199
0.973IleTyr: 0.973 ± 0.266
0.0IleXaa: 0.0 ± 0.0
Lys
5.131LysAla: 5.131 ± 0.76
0.31LysCys: 0.31 ± 0.113
3.185LysAsp: 3.185 ± 0.549
2.698LysGlu: 2.698 ± 0.432
2.035LysPhe: 2.035 ± 0.273
3.45LysGly: 3.45 ± 0.508
0.752LysHis: 0.752 ± 0.193
2.698LysIle: 2.698 ± 0.322
3.141LysLys: 3.141 ± 0.37
4.556LysLeu: 4.556 ± 0.402
1.194LysMet: 1.194 ± 0.234
2.477LysAsn: 2.477 ± 0.282
2.345LysPro: 2.345 ± 0.435
1.946LysGln: 1.946 ± 0.294
3.318LysArg: 3.318 ± 0.443
2.389LysSer: 2.389 ± 0.465
2.831LysThr: 2.831 ± 0.324
3.672LysVal: 3.672 ± 0.341
0.531LysTrp: 0.531 ± 0.155
1.548LysTyr: 1.548 ± 0.227
0.0LysXaa: 0.0 ± 0.0
Leu
6.768LeuAla: 6.768 ± 0.728
0.664LeuCys: 0.664 ± 0.182
4.512LeuAsp: 4.512 ± 0.415
6.458LeuGlu: 6.458 ± 0.75
2.787LeuPhe: 2.787 ± 0.356
5.043LeuGly: 5.043 ± 0.471
1.283LeuHis: 1.283 ± 0.237
3.495LeuIle: 3.495 ± 0.401
3.672LeuLys: 3.672 ± 0.479
5.353LeuLeu: 5.353 ± 0.449
2.079LeuMet: 2.079 ± 0.279
2.964LeuAsn: 2.964 ± 0.336
3.893LeuPro: 3.893 ± 0.414
3.185LeuGln: 3.185 ± 0.49
4.335LeuArg: 4.335 ± 0.455
4.114LeuSer: 4.114 ± 0.419
4.689LeuThr: 4.689 ± 0.486
4.601LeuVal: 4.601 ± 0.413
1.327LeuTrp: 1.327 ± 0.2
1.681LeuTyr: 1.681 ± 0.355
0.0LeuXaa: 0.0 ± 0.0
Met
2.433MetAla: 2.433 ± 0.361
0.31MetCys: 0.31 ± 0.13
1.15MetAsp: 1.15 ± 0.213
1.283MetGlu: 1.283 ± 0.245
0.664MetPhe: 0.664 ± 0.178
1.592MetGly: 1.592 ± 0.278
0.398MetHis: 0.398 ± 0.157
0.929MetIle: 0.929 ± 0.205
1.681MetLys: 1.681 ± 0.29
1.769MetLeu: 1.769 ± 0.276
0.664MetMet: 0.664 ± 0.161
1.239MetAsn: 1.239 ± 0.241
1.416MetPro: 1.416 ± 0.27
1.017MetGln: 1.017 ± 0.239
1.15MetArg: 1.15 ± 0.21
1.902MetSer: 1.902 ± 0.228
2.035MetThr: 2.035 ± 0.276
1.637MetVal: 1.637 ± 0.376
0.177MetTrp: 0.177 ± 0.062
0.973MetTyr: 0.973 ± 0.202
0.0MetXaa: 0.0 ± 0.0
Asn
4.114AsnAla: 4.114 ± 0.627
0.354AsnCys: 0.354 ± 0.163
2.3AsnAsp: 2.3 ± 0.35
1.991AsnGlu: 1.991 ± 0.287
1.194AsnPhe: 1.194 ± 0.169
4.291AsnGly: 4.291 ± 0.398
0.575AsnHis: 0.575 ± 0.145
2.389AsnIle: 2.389 ± 0.281
1.991AsnLys: 1.991 ± 0.299
3.008AsnLeu: 3.008 ± 0.364
1.017AsnMet: 1.017 ± 0.183
1.902AsnAsn: 1.902 ± 0.268
2.654AsnPro: 2.654 ± 0.28
1.769AsnGln: 1.769 ± 0.219
2.123AsnArg: 2.123 ± 0.325
2.389AsnSer: 2.389 ± 0.305
2.256AsnThr: 2.256 ± 0.287
2.698AsnVal: 2.698 ± 0.311
0.487AsnTrp: 0.487 ± 0.137
1.327AsnTyr: 1.327 ± 0.245
0.0AsnXaa: 0.0 ± 0.0
Pro
4.91ProAla: 4.91 ± 0.435
0.354ProCys: 0.354 ± 0.142
3.716ProAsp: 3.716 ± 0.427
4.291ProGlu: 4.291 ± 0.628
1.902ProPhe: 1.902 ± 0.302
4.822ProGly: 4.822 ± 0.576
0.84ProHis: 0.84 ± 0.228
2.168ProIle: 2.168 ± 0.289
2.743ProLys: 2.743 ± 0.392
2.831ProLeu: 2.831 ± 0.329
1.194ProMet: 1.194 ± 0.166
2.477ProAsn: 2.477 ± 0.404
2.61ProPro: 2.61 ± 0.396
1.814ProGln: 1.814 ± 0.373
1.46ProArg: 1.46 ± 0.284
3.406ProSer: 3.406 ± 0.492
3.185ProThr: 3.185 ± 0.494
4.335ProVal: 4.335 ± 0.414
1.062ProTrp: 1.062 ± 0.218
1.637ProTyr: 1.637 ± 0.281
0.0ProXaa: 0.0 ± 0.0
Gln
4.291GlnAla: 4.291 ± 0.625
0.221GlnCys: 0.221 ± 0.09
1.548GlnAsp: 1.548 ± 0.3
2.477GlnGlu: 2.477 ± 0.452
1.548GlnPhe: 1.548 ± 0.248
2.168GlnGly: 2.168 ± 0.282
0.664GlnHis: 0.664 ± 0.156
2.168GlnIle: 2.168 ± 0.354
1.637GlnLys: 1.637 ± 0.301
3.008GlnLeu: 3.008 ± 0.366
0.708GlnMet: 0.708 ± 0.163
1.371GlnAsn: 1.371 ± 0.329
1.769GlnPro: 1.769 ± 0.32
1.637GlnGln: 1.637 ± 0.264
2.566GlnArg: 2.566 ± 0.42
1.991GlnSer: 1.991 ± 0.306
2.035GlnThr: 2.035 ± 0.275
3.273GlnVal: 3.273 ± 0.307
0.752GlnTrp: 0.752 ± 0.199
1.15GlnTyr: 1.15 ± 0.229
0.0GlnXaa: 0.0 ± 0.0
Arg
4.822ArgAla: 4.822 ± 0.459
0.796ArgCys: 0.796 ± 0.197
3.052ArgAsp: 3.052 ± 0.522
4.025ArgGlu: 4.025 ± 0.49
1.991ArgPhe: 1.991 ± 0.253
3.627ArgGly: 3.627 ± 0.397
1.327ArgHis: 1.327 ± 0.226
3.229ArgIle: 3.229 ± 0.405
2.698ArgLys: 2.698 ± 0.368
5.131ArgLeu: 5.131 ± 0.527
1.637ArgMet: 1.637 ± 0.28
2.521ArgAsn: 2.521 ± 0.331
3.052ArgPro: 3.052 ± 0.429
2.345ArgGln: 2.345 ± 0.288
4.645ArgArg: 4.645 ± 0.641
2.477ArgSer: 2.477 ± 0.325
2.743ArgThr: 2.743 ± 0.361
4.424ArgVal: 4.424 ± 0.621
0.664ArgTrp: 0.664 ± 0.205
2.168ArgTyr: 2.168 ± 0.31
0.0ArgXaa: 0.0 ± 0.0
Ser
5.972SerAla: 5.972 ± 0.547
0.31SerCys: 0.31 ± 0.118
3.937SerAsp: 3.937 ± 0.357
3.141SerGlu: 3.141 ± 0.383
2.345SerPhe: 2.345 ± 0.331
4.689SerGly: 4.689 ± 0.558
1.106SerHis: 1.106 ± 0.191
4.247SerIle: 4.247 ± 0.477
3.097SerLys: 3.097 ± 0.353
3.981SerLeu: 3.981 ± 0.479
1.725SerMet: 1.725 ± 0.259
2.389SerAsn: 2.389 ± 0.313
3.185SerPro: 3.185 ± 0.336
1.548SerGln: 1.548 ± 0.256
3.495SerArg: 3.495 ± 0.298
4.91SerSer: 4.91 ± 0.528
3.627SerThr: 3.627 ± 0.333
4.556SerVal: 4.556 ± 0.461
0.973SerTrp: 0.973 ± 0.179
1.902SerTyr: 1.902 ± 0.264
0.0SerXaa: 0.0 ± 0.0
Thr
6.458ThrAla: 6.458 ± 0.792
0.531ThrCys: 0.531 ± 0.189
3.76ThrAsp: 3.76 ± 0.554
3.583ThrGlu: 3.583 ± 0.414
1.902ThrPhe: 1.902 ± 0.261
5.751ThrGly: 5.751 ± 0.582
0.752ThrHis: 0.752 ± 0.205
2.787ThrIle: 2.787 ± 0.395
2.433ThrLys: 2.433 ± 0.312
4.379ThrLeu: 4.379 ± 0.419
1.062ThrMet: 1.062 ± 0.243
2.345ThrAsn: 2.345 ± 0.339
4.202ThrPro: 4.202 ± 0.617
1.46ThrGln: 1.46 ± 0.245
2.654ThrArg: 2.654 ± 0.362
4.07ThrSer: 4.07 ± 0.424
4.645ThrThr: 4.645 ± 0.566
5.264ThrVal: 5.264 ± 0.513
1.327ThrTrp: 1.327 ± 0.22
1.814ThrTyr: 1.814 ± 0.304
0.0ThrXaa: 0.0 ± 0.0
Val
6.901ValAla: 6.901 ± 0.767
0.619ValCys: 0.619 ± 0.23
4.424ValAsp: 4.424 ± 0.443
5.618ValGlu: 5.618 ± 0.434
3.097ValPhe: 3.097 ± 0.383
5.043ValGly: 5.043 ± 0.57
1.592ValHis: 1.592 ± 0.258
3.716ValIle: 3.716 ± 0.47
3.318ValLys: 3.318 ± 0.348
4.954ValLeu: 4.954 ± 0.473
1.548ValMet: 1.548 ± 0.255
2.831ValAsn: 2.831 ± 0.317
3.804ValPro: 3.804 ± 0.47
2.123ValGln: 2.123 ± 0.325
4.379ValArg: 4.379 ± 0.456
5.043ValSer: 5.043 ± 0.669
4.645ValThr: 4.645 ± 0.618
5.087ValVal: 5.087 ± 0.501
1.327ValTrp: 1.327 ± 0.211
1.548ValTyr: 1.548 ± 0.223
0.0ValXaa: 0.0 ± 0.0
Trp
1.592TrpAla: 1.592 ± 0.463
0.354TrpCys: 0.354 ± 0.109
1.239TrpAsp: 1.239 ± 0.264
1.327TrpGlu: 1.327 ± 0.206
0.796TrpPhe: 0.796 ± 0.181
1.283TrpGly: 1.283 ± 0.215
0.354TrpHis: 0.354 ± 0.119
0.664TrpIle: 0.664 ± 0.21
0.973TrpLys: 0.973 ± 0.159
1.062TrpLeu: 1.062 ± 0.209
0.265TrpMet: 0.265 ± 0.088
0.664TrpAsn: 0.664 ± 0.22
0.752TrpPro: 0.752 ± 0.146
0.708TrpGln: 0.708 ± 0.188
1.327TrpArg: 1.327 ± 0.288
0.973TrpSer: 0.973 ± 0.214
1.194TrpThr: 1.194 ± 0.281
0.885TrpVal: 0.885 ± 0.159
0.177TrpTrp: 0.177 ± 0.101
0.487TrpTyr: 0.487 ± 0.134
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.477TyrAla: 2.477 ± 0.363
0.487TyrCys: 0.487 ± 0.147
1.858TyrAsp: 1.858 ± 0.327
1.858TyrGlu: 1.858 ± 0.309
1.15TyrPhe: 1.15 ± 0.215
2.566TyrGly: 2.566 ± 0.299
0.398TyrHis: 0.398 ± 0.123
1.416TyrIle: 1.416 ± 0.293
1.416TyrLys: 1.416 ± 0.224
2.212TyrLeu: 2.212 ± 0.318
0.885TyrMet: 0.885 ± 0.199
1.592TyrAsn: 1.592 ± 0.311
1.548TyrPro: 1.548 ± 0.276
1.106TyrGln: 1.106 ± 0.24
2.079TyrArg: 2.079 ± 0.348
1.371TyrSer: 1.371 ± 0.243
1.902TyrThr: 1.902 ± 0.313
2.079TyrVal: 2.079 ± 0.44
0.531TyrTrp: 0.531 ± 0.175
0.752TyrTyr: 0.752 ± 0.176
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (22607 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski