Amino acid dipepetide frequency for Streptococcus phage phiARI0004

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.243AlaAla: 2.243 ± 0.651
0.179AlaCys: 0.179 ± 0.144
5.473AlaAsp: 5.473 ± 0.641
6.819AlaGlu: 6.819 ± 0.9
2.692AlaPhe: 2.692 ± 0.624
6.28AlaGly: 6.28 ± 1.161
0.449AlaHis: 0.449 ± 0.176
4.576AlaIle: 4.576 ± 0.885
5.473AlaLys: 5.473 ± 0.793
6.819AlaLeu: 6.819 ± 0.878
2.422AlaMet: 2.422 ± 0.446
3.768AlaAsn: 3.768 ± 0.775
1.794AlaPro: 1.794 ± 0.386
2.961AlaGln: 2.961 ± 0.526
2.871AlaArg: 2.871 ± 0.711
2.512AlaSer: 2.512 ± 0.928
4.935AlaThr: 4.935 ± 0.842
5.204AlaVal: 5.204 ± 0.787
1.346AlaTrp: 1.346 ± 0.46
2.333AlaTyr: 2.333 ± 0.355
0.0AlaXaa: 0.0 ± 0.0
Cys
0.359CysAla: 0.359 ± 0.161
0.179CysCys: 0.179 ± 0.127
0.359CysAsp: 0.359 ± 0.232
0.359CysGlu: 0.359 ± 0.188
0.09CysPhe: 0.09 ± 0.061
0.449CysGly: 0.449 ± 0.331
0.179CysHis: 0.179 ± 0.103
0.449CysIle: 0.449 ± 0.223
0.807CysLys: 0.807 ± 0.325
0.179CysLeu: 0.179 ± 0.114
0.179CysMet: 0.179 ± 0.14
0.269CysAsn: 0.269 ± 0.208
0.179CysPro: 0.179 ± 0.121
0.359CysGln: 0.359 ± 0.16
0.538CysArg: 0.538 ± 0.242
0.269CysSer: 0.269 ± 0.161
0.269CysThr: 0.269 ± 0.181
0.0CysVal: 0.0 ± 0.0
0.179CysTrp: 0.179 ± 0.1
0.269CysTyr: 0.269 ± 0.154
0.0CysXaa: 0.0 ± 0.0
Asp
3.499AspAla: 3.499 ± 0.727
0.538AspCys: 0.538 ± 0.269
3.23AspAsp: 3.23 ± 0.733
5.293AspGlu: 5.293 ± 1.226
2.961AspPhe: 2.961 ± 0.566
5.114AspGly: 5.114 ± 0.612
0.179AspHis: 0.179 ± 0.131
4.845AspIle: 4.845 ± 0.483
4.665AspLys: 4.665 ± 0.907
5.921AspLeu: 5.921 ± 0.647
1.794AspMet: 1.794 ± 0.382
3.05AspAsn: 3.05 ± 0.461
2.243AspPro: 2.243 ± 0.549
1.705AspGln: 1.705 ± 0.439
2.781AspArg: 2.781 ± 0.477
3.32AspSer: 3.32 ± 0.47
4.037AspThr: 4.037 ± 0.573
4.127AspVal: 4.127 ± 0.595
1.435AspTrp: 1.435 ± 0.404
3.23AspTyr: 3.23 ± 0.563
0.0AspXaa: 0.0 ± 0.0
Glu
6.639GluAla: 6.639 ± 0.841
0.359GluCys: 0.359 ± 0.201
4.037GluAsp: 4.037 ± 0.692
6.729GluGlu: 6.729 ± 1.432
4.127GluPhe: 4.127 ± 0.821
4.127GluGly: 4.127 ± 0.712
0.987GluHis: 0.987 ± 0.315
6.549GluIle: 6.549 ± 0.848
5.204GluLys: 5.204 ± 1.057
9.869GluLeu: 9.869 ± 1.151
1.615GluMet: 1.615 ± 0.449
3.948GluAsn: 3.948 ± 0.559
1.077GluPro: 1.077 ± 0.387
4.037GluGln: 4.037 ± 0.958
4.127GluArg: 4.127 ± 0.636
4.845GluSer: 4.845 ± 0.476
4.037GluThr: 4.037 ± 0.636
6.37GluVal: 6.37 ± 0.977
1.077GluTrp: 1.077 ± 0.294
2.871GluTyr: 2.871 ± 0.535
0.0GluXaa: 0.0 ± 0.0
Phe
2.512PheAla: 2.512 ± 0.573
0.179PheCys: 0.179 ± 0.124
4.576PheAsp: 4.576 ± 0.627
4.217PheGlu: 4.217 ± 0.798
1.256PhePhe: 1.256 ± 0.372
2.692PheGly: 2.692 ± 0.552
0.449PheHis: 0.449 ± 0.262
2.243PheIle: 2.243 ± 0.505
3.768PheLys: 3.768 ± 0.738
2.064PheLeu: 2.064 ± 0.403
1.435PheMet: 1.435 ± 0.456
2.512PheAsn: 2.512 ± 0.462
0.807PhePro: 0.807 ± 0.252
1.794PheGln: 1.794 ± 0.398
0.897PheArg: 0.897 ± 0.225
3.768PheSer: 3.768 ± 0.659
2.781PheThr: 2.781 ± 0.546
1.346PheVal: 1.346 ± 0.229
0.359PheTrp: 0.359 ± 0.158
2.064PheTyr: 2.064 ± 0.447
0.0PheXaa: 0.0 ± 0.0
Gly
3.409GlyAla: 3.409 ± 0.524
0.09GlyCys: 0.09 ± 0.105
4.037GlyAsp: 4.037 ± 0.664
4.665GlyGlu: 4.665 ± 0.818
2.871GlyPhe: 2.871 ± 0.509
5.024GlyGly: 5.024 ± 1.129
0.897GlyHis: 0.897 ± 0.253
3.589GlyIle: 3.589 ± 0.617
4.755GlyLys: 4.755 ± 0.494
5.921GlyLeu: 5.921 ± 1.16
1.794GlyMet: 1.794 ± 0.376
3.499GlyAsn: 3.499 ± 0.491
0.718GlyPro: 0.718 ± 0.255
3.948GlyGln: 3.948 ± 0.57
3.499GlyArg: 3.499 ± 0.696
4.576GlySer: 4.576 ± 0.781
3.768GlyThr: 3.768 ± 0.59
4.396GlyVal: 4.396 ± 0.69
1.166GlyTrp: 1.166 ± 0.496
3.32GlyTyr: 3.32 ± 0.64
0.0GlyXaa: 0.0 ± 0.0
His
0.807HisAla: 0.807 ± 0.299
0.09HisCys: 0.09 ± 0.11
0.538HisAsp: 0.538 ± 0.229
1.166HisGlu: 1.166 ± 0.327
0.897HisPhe: 0.897 ± 0.263
0.987HisGly: 0.987 ± 0.305
0.09HisHis: 0.09 ± 0.105
0.538HisIle: 0.538 ± 0.315
0.628HisLys: 0.628 ± 0.234
0.897HisLeu: 0.897 ± 0.241
0.269HisMet: 0.269 ± 0.197
0.807HisAsn: 0.807 ± 0.259
0.538HisPro: 0.538 ± 0.193
0.449HisGln: 0.449 ± 0.231
0.538HisArg: 0.538 ± 0.24
1.346HisSer: 1.346 ± 0.467
0.359HisThr: 0.359 ± 0.178
0.449HisVal: 0.449 ± 0.233
0.449HisTrp: 0.449 ± 0.196
0.538HisTyr: 0.538 ± 0.266
0.0HisXaa: 0.0 ± 0.0
Ile
5.383IleAla: 5.383 ± 0.908
0.359IleCys: 0.359 ± 0.132
3.858IleAsp: 3.858 ± 0.634
6.28IleGlu: 6.28 ± 0.764
3.32IlePhe: 3.32 ± 0.58
4.755IleGly: 4.755 ± 0.814
0.538IleHis: 0.538 ± 0.236
3.32IleIle: 3.32 ± 0.66
6.101IleLys: 6.101 ± 0.929
3.948IleLeu: 3.948 ± 0.574
1.256IleMet: 1.256 ± 0.376
3.678IleAsn: 3.678 ± 0.596
1.705IlePro: 1.705 ± 0.371
2.153IleGln: 2.153 ± 0.424
2.961IleArg: 2.961 ± 0.761
5.204IleSer: 5.204 ± 0.58
4.306IleThr: 4.306 ± 0.56
3.23IleVal: 3.23 ± 0.569
0.538IleTrp: 0.538 ± 0.209
2.064IleTyr: 2.064 ± 0.582
0.0IleXaa: 0.0 ± 0.0
Lys
5.204LysAla: 5.204 ± 0.894
0.807LysCys: 0.807 ± 0.354
6.28LysAsp: 6.28 ± 0.669
6.729LysGlu: 6.729 ± 0.733
2.781LysPhe: 2.781 ± 0.51
4.127LysGly: 4.127 ± 0.583
1.166LysHis: 1.166 ± 0.329
5.473LysIle: 5.473 ± 0.832
6.819LysLys: 6.819 ± 0.922
7.536LysLeu: 7.536 ± 0.747
2.333LysMet: 2.333 ± 0.442
5.383LysAsn: 5.383 ± 0.825
2.333LysPro: 2.333 ± 0.625
3.948LysGln: 3.948 ± 0.68
3.858LysArg: 3.858 ± 0.55
4.306LysSer: 4.306 ± 0.758
4.127LysThr: 4.127 ± 0.587
5.114LysVal: 5.114 ± 0.658
0.897LysTrp: 0.897 ± 0.385
3.14LysTyr: 3.14 ± 0.658
0.0LysXaa: 0.0 ± 0.0
Leu
6.549LeuAla: 6.549 ± 0.788
0.628LeuCys: 0.628 ± 0.314
6.101LeuAsp: 6.101 ± 0.885
7.088LeuGlu: 7.088 ± 1.007
2.692LeuPhe: 2.692 ± 0.506
5.652LeuGly: 5.652 ± 1.332
1.077LeuHis: 1.077 ± 0.304
4.486LeuIle: 4.486 ± 0.58
7.716LeuLys: 7.716 ± 0.936
6.28LeuLeu: 6.28 ± 0.916
1.615LeuMet: 1.615 ± 0.43
4.127LeuAsn: 4.127 ± 0.69
2.333LeuPro: 2.333 ± 0.692
2.153LeuGln: 2.153 ± 0.517
4.665LeuArg: 4.665 ± 0.687
5.204LeuSer: 5.204 ± 1.136
5.473LeuThr: 5.473 ± 0.786
3.768LeuVal: 3.768 ± 0.691
0.269LeuTrp: 0.269 ± 0.192
2.602LeuTyr: 2.602 ± 0.323
0.0LeuXaa: 0.0 ± 0.0
Met
1.884MetAla: 1.884 ± 0.446
0.0MetCys: 0.0 ± 0.0
1.077MetAsp: 1.077 ± 0.238
1.705MetGlu: 1.705 ± 0.47
0.987MetPhe: 0.987 ± 0.305
1.435MetGly: 1.435 ± 0.45
0.269MetHis: 0.269 ± 0.166
1.705MetIle: 1.705 ± 0.334
2.243MetLys: 2.243 ± 0.58
1.615MetLeu: 1.615 ± 0.365
0.449MetMet: 0.449 ± 0.251
1.166MetAsn: 1.166 ± 0.414
0.807MetPro: 0.807 ± 0.282
0.718MetGln: 0.718 ± 0.323
0.897MetArg: 0.897 ± 0.324
1.435MetSer: 1.435 ± 0.563
1.884MetThr: 1.884 ± 0.498
1.346MetVal: 1.346 ± 0.313
0.179MetTrp: 0.179 ± 0.144
0.718MetTyr: 0.718 ± 0.246
0.0MetXaa: 0.0 ± 0.0
Asn
4.576AsnAla: 4.576 ± 0.871
0.269AsnCys: 0.269 ± 0.171
2.961AsnAsp: 2.961 ± 0.507
3.499AsnGlu: 3.499 ± 0.655
2.153AsnPhe: 2.153 ± 0.499
4.037AsnGly: 4.037 ± 0.618
1.166AsnHis: 1.166 ± 0.392
3.23AsnIle: 3.23 ± 0.369
4.127AsnLys: 4.127 ± 0.572
3.678AsnLeu: 3.678 ± 0.748
1.077AsnMet: 1.077 ± 0.292
1.974AsnAsn: 1.974 ± 0.506
2.512AsnPro: 2.512 ± 0.402
2.961AsnGln: 2.961 ± 0.61
2.243AsnArg: 2.243 ± 0.446
3.05AsnSer: 3.05 ± 0.761
2.781AsnThr: 2.781 ± 0.505
3.05AsnVal: 3.05 ± 0.471
0.807AsnTrp: 0.807 ± 0.196
2.243AsnTyr: 2.243 ± 0.397
0.0AsnXaa: 0.0 ± 0.0
Pro
2.422ProAla: 2.422 ± 0.498
0.09ProCys: 0.09 ± 0.102
2.243ProAsp: 2.243 ± 0.501
2.781ProGlu: 2.781 ± 0.46
1.346ProPhe: 1.346 ± 0.497
0.807ProGly: 0.807 ± 0.178
0.179ProHis: 0.179 ± 0.112
1.525ProIle: 1.525 ± 0.559
3.05ProLys: 3.05 ± 0.444
1.077ProLeu: 1.077 ± 0.353
0.449ProMet: 0.449 ± 0.207
1.346ProAsn: 1.346 ± 0.394
0.807ProPro: 0.807 ± 0.335
0.897ProGln: 0.897 ± 0.327
1.435ProArg: 1.435 ± 0.393
0.987ProSer: 0.987 ± 0.337
0.987ProThr: 0.987 ± 0.286
1.794ProVal: 1.794 ± 0.343
0.538ProTrp: 0.538 ± 0.201
1.525ProTyr: 1.525 ± 0.495
0.0ProXaa: 0.0 ± 0.0
Gln
4.037GlnAla: 4.037 ± 0.568
0.359GlnCys: 0.359 ± 0.201
1.974GlnAsp: 1.974 ± 0.421
4.396GlnGlu: 4.396 ± 0.874
1.525GlnPhe: 1.525 ± 0.377
2.153GlnGly: 2.153 ± 0.417
0.359GlnHis: 0.359 ± 0.166
2.961GlnIle: 2.961 ± 0.613
3.32GlnLys: 3.32 ± 0.509
2.781GlnLeu: 2.781 ± 0.528
0.628GlnMet: 0.628 ± 0.211
2.064GlnAsn: 2.064 ± 0.333
1.077GlnPro: 1.077 ± 0.334
1.525GlnGln: 1.525 ± 0.46
2.243GlnArg: 2.243 ± 0.468
2.602GlnSer: 2.602 ± 0.361
3.499GlnThr: 3.499 ± 0.612
3.409GlnVal: 3.409 ± 0.568
0.538GlnTrp: 0.538 ± 0.182
1.256GlnTyr: 1.256 ± 0.345
0.0GlnXaa: 0.0 ± 0.0
Arg
3.499ArgAla: 3.499 ± 0.504
0.449ArgCys: 0.449 ± 0.192
2.512ArgAsp: 2.512 ± 0.508
2.961ArgGlu: 2.961 ± 0.854
1.884ArgPhe: 1.884 ± 0.448
1.974ArgGly: 1.974 ± 0.524
0.807ArgHis: 0.807 ± 0.22
3.948ArgIle: 3.948 ± 0.508
3.32ArgLys: 3.32 ± 0.925
4.665ArgLeu: 4.665 ± 0.706
1.256ArgMet: 1.256 ± 0.302
2.602ArgAsn: 2.602 ± 0.599
1.166ArgPro: 1.166 ± 0.334
3.05ArgGln: 3.05 ± 0.667
2.422ArgArg: 2.422 ± 0.523
2.153ArgSer: 2.153 ± 0.523
2.871ArgThr: 2.871 ± 0.796
2.512ArgVal: 2.512 ± 0.438
0.538ArgTrp: 0.538 ± 0.193
1.705ArgTyr: 1.705 ± 0.451
0.0ArgXaa: 0.0 ± 0.0
Ser
4.665SerAla: 4.665 ± 0.971
0.09SerCys: 0.09 ± 0.061
3.409SerAsp: 3.409 ± 0.637
4.217SerGlu: 4.217 ± 0.553
1.884SerPhe: 1.884 ± 0.383
4.935SerGly: 4.935 ± 0.661
0.897SerHis: 0.897 ± 0.418
4.396SerIle: 4.396 ± 0.807
4.486SerLys: 4.486 ± 0.565
4.755SerLeu: 4.755 ± 0.688
0.987SerMet: 0.987 ± 0.406
3.409SerAsn: 3.409 ± 0.537
1.525SerPro: 1.525 ± 0.345
2.333SerGln: 2.333 ± 0.472
3.499SerArg: 3.499 ± 0.793
3.768SerSer: 3.768 ± 0.865
4.217SerThr: 4.217 ± 0.594
3.678SerVal: 3.678 ± 0.78
0.987SerTrp: 0.987 ± 0.383
2.781SerTyr: 2.781 ± 0.527
0.0SerXaa: 0.0 ± 0.0
Thr
5.563ThrAla: 5.563 ± 0.999
0.179ThrCys: 0.179 ± 0.123
4.755ThrAsp: 4.755 ± 0.657
4.217ThrGlu: 4.217 ± 0.503
3.05ThrPhe: 3.05 ± 0.614
3.768ThrGly: 3.768 ± 0.76
0.449ThrHis: 0.449 ± 0.207
4.306ThrIle: 4.306 ± 0.656
4.755ThrLys: 4.755 ± 0.886
4.845ThrLeu: 4.845 ± 0.624
0.718ThrMet: 0.718 ± 0.328
2.781ThrAsn: 2.781 ± 0.451
1.166ThrPro: 1.166 ± 0.336
3.32ThrGln: 3.32 ± 0.669
1.525ThrArg: 1.525 ± 0.29
3.678ThrSer: 3.678 ± 0.55
4.396ThrThr: 4.396 ± 0.849
4.217ThrVal: 4.217 ± 0.67
0.987ThrTrp: 0.987 ± 0.285
2.602ThrTyr: 2.602 ± 0.51
0.0ThrXaa: 0.0 ± 0.0
Val
4.755ValAla: 4.755 ± 0.745
0.179ValCys: 0.179 ± 0.124
3.409ValAsp: 3.409 ± 0.52
5.473ValGlu: 5.473 ± 0.727
2.333ValPhe: 2.333 ± 0.462
4.845ValGly: 4.845 ± 0.767
1.256ValHis: 1.256 ± 0.365
3.14ValIle: 3.14 ± 0.615
5.563ValLys: 5.563 ± 0.677
3.499ValLeu: 3.499 ± 0.6
1.166ValMet: 1.166 ± 0.324
3.858ValAsn: 3.858 ± 0.718
2.064ValPro: 2.064 ± 0.338
1.435ValGln: 1.435 ± 0.333
2.602ValArg: 2.602 ± 0.35
5.204ValSer: 5.204 ± 0.739
3.948ValThr: 3.948 ± 0.601
4.217ValVal: 4.217 ± 0.87
0.807ValTrp: 0.807 ± 0.282
2.243ValTyr: 2.243 ± 0.521
0.0ValXaa: 0.0 ± 0.0
Trp
1.166TrpAla: 1.166 ± 0.434
0.179TrpCys: 0.179 ± 0.111
0.897TrpAsp: 0.897 ± 0.349
0.718TrpGlu: 0.718 ± 0.259
0.897TrpPhe: 0.897 ± 0.451
0.718TrpGly: 0.718 ± 0.244
0.09TrpHis: 0.09 ± 0.105
0.628TrpIle: 0.628 ± 0.251
1.525TrpLys: 1.525 ± 0.486
0.718TrpLeu: 0.718 ± 0.363
0.269TrpMet: 0.269 ± 0.149
0.807TrpAsn: 0.807 ± 0.275
0.0TrpPro: 0.0 ± 0.0
0.628TrpGln: 0.628 ± 0.28
0.718TrpArg: 0.718 ± 0.316
0.538TrpSer: 0.538 ± 0.225
1.077TrpThr: 1.077 ± 0.289
1.077TrpVal: 1.077 ± 0.251
0.179TrpTrp: 0.179 ± 0.121
0.897TrpTyr: 0.897 ± 0.548
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.974TyrAla: 1.974 ± 0.345
0.718TyrCys: 0.718 ± 0.342
2.243TyrAsp: 2.243 ± 0.463
3.32TyrGlu: 3.32 ± 0.63
1.974TyrPhe: 1.974 ± 0.446
2.064TyrGly: 2.064 ± 0.353
0.897TyrHis: 0.897 ± 0.253
3.14TyrIle: 3.14 ± 0.68
4.037TyrLys: 4.037 ± 0.661
3.409TyrLeu: 3.409 ± 0.749
0.718TyrMet: 0.718 ± 0.316
1.346TyrAsn: 1.346 ± 0.346
1.435TyrPro: 1.435 ± 0.425
2.333TyrGln: 2.333 ± 0.5
1.974TyrArg: 1.974 ± 0.528
2.153TyrSer: 2.153 ± 0.577
1.525TyrThr: 1.525 ± 0.276
2.692TyrVal: 2.692 ± 0.541
0.449TyrTrp: 0.449 ± 0.212
1.974TyrTyr: 1.974 ± 0.552
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (11147 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski