Amino acid dipepetide frequency for Mycobacterium virus Rumpelstiltskin

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.791AlaAla: 12.791 ± 1.229
0.9AlaCys: 0.9 ± 0.228
6.538AlaAsp: 6.538 ± 0.661
7.485AlaGlu: 7.485 ± 0.626
3.222AlaPhe: 3.222 ± 0.372
7.959AlaGly: 7.959 ± 0.825
1.895AlaHis: 1.895 ± 0.318
4.548AlaIle: 4.548 ± 0.432
6.396AlaLys: 6.396 ± 0.586
9.617AlaLeu: 9.617 ± 0.609
3.127AlaMet: 3.127 ± 0.429
3.932AlaAsn: 3.932 ± 0.602
4.69AlaPro: 4.69 ± 0.686
4.359AlaGln: 4.359 ± 0.725
5.59AlaArg: 5.59 ± 0.465
5.638AlaSer: 5.638 ± 0.643
5.732AlaThr: 5.732 ± 0.683
8.101AlaVal: 8.101 ± 0.987
1.516AlaTrp: 1.516 ± 0.28
2.795AlaTyr: 2.795 ± 0.289
0.0AlaXaa: 0.0 ± 0.0
Cys
0.663CysAla: 0.663 ± 0.156
0.284CysCys: 0.284 ± 0.127
0.995CysAsp: 0.995 ± 0.213
0.663CysGlu: 0.663 ± 0.169
0.332CysPhe: 0.332 ± 0.118
1.279CysGly: 1.279 ± 0.276
0.142CysHis: 0.142 ± 0.082
0.142CysIle: 0.142 ± 0.089
0.237CysLys: 0.237 ± 0.117
0.663CysLeu: 0.663 ± 0.183
0.284CysMet: 0.284 ± 0.112
0.379CysAsn: 0.379 ± 0.158
0.474CysPro: 0.474 ± 0.131
0.474CysGln: 0.474 ± 0.185
0.758CysArg: 0.758 ± 0.249
0.426CysSer: 0.426 ± 0.144
0.569CysThr: 0.569 ± 0.168
0.758CysVal: 0.758 ± 0.212
0.19CysTrp: 0.19 ± 0.103
0.142CysTyr: 0.142 ± 0.075
0.0CysXaa: 0.0 ± 0.0
Asp
5.069AspAla: 5.069 ± 0.642
0.853AspCys: 0.853 ± 0.23
4.595AspAsp: 4.595 ± 0.536
3.98AspGlu: 3.98 ± 0.566
2.227AspPhe: 2.227 ± 0.291
5.59AspGly: 5.59 ± 0.471
0.948AspHis: 0.948 ± 0.224
3.127AspIle: 3.127 ± 0.486
3.316AspLys: 3.316 ± 0.41
5.59AspLeu: 5.59 ± 0.468
1.611AspMet: 1.611 ± 0.314
2.085AspAsn: 2.085 ± 0.356
3.79AspPro: 3.79 ± 0.526
2.227AspGln: 2.227 ± 0.203
4.311AspArg: 4.311 ± 0.477
3.364AspSer: 3.364 ± 0.49
3.079AspThr: 3.079 ± 0.406
3.79AspVal: 3.79 ± 0.522
1.516AspTrp: 1.516 ± 0.367
2.558AspTyr: 2.558 ± 0.439
0.0AspXaa: 0.0 ± 0.0
Glu
6.585GluAla: 6.585 ± 0.579
0.569GluCys: 0.569 ± 0.187
2.653GluAsp: 2.653 ± 0.352
2.085GluGlu: 2.085 ± 0.384
1.99GluPhe: 1.99 ± 0.287
4.122GluGly: 4.122 ± 0.428
1.99GluHis: 1.99 ± 0.279
2.653GluIle: 2.653 ± 0.316
2.89GluLys: 2.89 ± 0.466
7.106GluLeu: 7.106 ± 0.589
1.421GluMet: 1.421 ± 0.214
2.085GluAsn: 2.085 ± 0.303
2.558GluPro: 2.558 ± 0.424
2.132GluGln: 2.132 ± 0.323
4.832GluArg: 4.832 ± 0.532
2.795GluSer: 2.795 ± 0.463
3.98GluThr: 3.98 ± 0.566
3.98GluVal: 3.98 ± 0.449
1.042GluTrp: 1.042 ± 0.255
1.99GluTyr: 1.99 ± 0.311
0.0GluXaa: 0.0 ± 0.0
Phe
2.843PheAla: 2.843 ± 0.328
0.426PheCys: 0.426 ± 0.142
1.8PheAsp: 1.8 ± 0.301
1.895PheGlu: 1.895 ± 0.293
1.327PhePhe: 1.327 ± 0.201
3.174PheGly: 3.174 ± 0.41
0.711PheHis: 0.711 ± 0.149
1.469PheIle: 1.469 ± 0.253
1.753PheLys: 1.753 ± 0.27
2.558PheLeu: 2.558 ± 0.41
0.853PheMet: 0.853 ± 0.199
1.184PheAsn: 1.184 ± 0.192
1.469PhePro: 1.469 ± 0.256
0.853PheGln: 0.853 ± 0.192
1.8PheArg: 1.8 ± 0.328
1.848PheSer: 1.848 ± 0.27
1.895PheThr: 1.895 ± 0.252
2.179PheVal: 2.179 ± 0.299
0.616PheTrp: 0.616 ± 0.149
1.232PheTyr: 1.232 ± 0.23
0.0PheXaa: 0.0 ± 0.0
Gly
7.58GlyAla: 7.58 ± 1.072
0.758GlyCys: 0.758 ± 0.198
5.543GlyAsp: 5.543 ± 0.528
5.875GlyGlu: 5.875 ± 0.493
2.89GlyPhe: 2.89 ± 0.477
8.812GlyGly: 8.812 ± 1.477
1.848GlyHis: 1.848 ± 0.325
4.311GlyIle: 4.311 ± 0.473
5.306GlyLys: 5.306 ± 0.505
7.296GlyLeu: 7.296 ± 0.87
1.706GlyMet: 1.706 ± 0.243
2.795GlyAsn: 2.795 ± 0.391
3.553GlyPro: 3.553 ± 0.317
2.464GlyGln: 2.464 ± 0.401
4.927GlyArg: 4.927 ± 0.449
5.259GlySer: 5.259 ± 0.645
5.353GlyThr: 5.353 ± 0.676
6.017GlyVal: 6.017 ± 0.631
1.658GlyTrp: 1.658 ± 0.29
2.511GlyTyr: 2.511 ± 0.305
0.0GlyXaa: 0.0 ± 0.0
His
1.848HisAla: 1.848 ± 0.261
0.142HisCys: 0.142 ± 0.08
0.9HisAsp: 0.9 ± 0.202
1.184HisGlu: 1.184 ± 0.277
0.948HisPhe: 0.948 ± 0.216
1.753HisGly: 1.753 ± 0.296
0.332HisHis: 0.332 ± 0.16
0.758HisIle: 0.758 ± 0.249
0.805HisLys: 0.805 ± 0.189
1.8HisLeu: 1.8 ± 0.294
0.521HisMet: 0.521 ± 0.179
0.474HisAsn: 0.474 ± 0.123
0.995HisPro: 0.995 ± 0.228
0.379HisGln: 0.379 ± 0.119
1.327HisArg: 1.327 ± 0.248
0.853HisSer: 0.853 ± 0.197
1.184HisThr: 1.184 ± 0.222
1.848HisVal: 1.848 ± 0.312
0.379HisTrp: 0.379 ± 0.138
0.711HisTyr: 0.711 ± 0.199
0.0HisXaa: 0.0 ± 0.0
Ile
4.785IleAla: 4.785 ± 0.441
0.379IleCys: 0.379 ± 0.172
2.937IleAsp: 2.937 ± 0.4
2.985IleGlu: 2.985 ± 0.404
1.137IlePhe: 1.137 ± 0.177
3.648IleGly: 3.648 ± 0.444
0.663IleHis: 0.663 ± 0.146
1.753IleIle: 1.753 ± 0.275
2.274IleLys: 2.274 ± 0.342
3.127IleLeu: 3.127 ± 0.37
0.805IleMet: 0.805 ± 0.159
1.469IleAsn: 1.469 ± 0.25
2.511IlePro: 2.511 ± 0.3
1.8IleGln: 1.8 ± 0.43
2.843IleArg: 2.843 ± 0.378
2.085IleSer: 2.085 ± 0.296
2.606IleThr: 2.606 ± 0.383
2.937IleVal: 2.937 ± 0.368
0.474IleTrp: 0.474 ± 0.18
0.948IleTyr: 0.948 ± 0.208
0.0IleXaa: 0.0 ± 0.0
Lys
6.633LysAla: 6.633 ± 0.663
0.616LysCys: 0.616 ± 0.189
2.843LysAsp: 2.843 ± 0.39
1.895LysGlu: 1.895 ± 0.369
1.753LysPhe: 1.753 ± 0.285
4.453LysGly: 4.453 ± 0.456
0.948LysHis: 0.948 ± 0.24
1.469LysIle: 1.469 ± 0.301
2.274LysLys: 2.274 ± 0.433
3.837LysLeu: 3.837 ± 0.51
0.805LysMet: 0.805 ± 0.186
2.321LysAsn: 2.321 ± 0.393
2.985LysPro: 2.985 ± 0.443
2.132LysGln: 2.132 ± 0.35
4.548LysArg: 4.548 ± 0.489
2.653LysSer: 2.653 ± 0.373
2.274LysThr: 2.274 ± 0.402
2.748LysVal: 2.748 ± 0.367
1.516LysTrp: 1.516 ± 0.265
1.706LysTyr: 1.706 ± 0.26
0.0LysXaa: 0.0 ± 0.0
Leu
9.38LeuAla: 9.38 ± 0.61
0.616LeuCys: 0.616 ± 0.163
6.064LeuAsp: 6.064 ± 0.485
5.353LeuGlu: 5.353 ± 0.581
2.795LeuPhe: 2.795 ± 0.399
5.543LeuGly: 5.543 ± 0.531
1.421LeuHis: 1.421 ± 0.275
3.506LeuIle: 3.506 ± 0.584
4.169LeuLys: 4.169 ± 0.404
7.059LeuLeu: 7.059 ± 0.46
1.706LeuMet: 1.706 ± 0.291
3.458LeuAsn: 3.458 ± 0.52
4.832LeuPro: 4.832 ± 0.68
2.89LeuGln: 2.89 ± 0.397
5.59LeuArg: 5.59 ± 0.488
5.496LeuSer: 5.496 ± 0.685
5.069LeuThr: 5.069 ± 0.495
5.922LeuVal: 5.922 ± 0.44
2.179LeuTrp: 2.179 ± 0.369
1.848LeuTyr: 1.848 ± 0.329
0.0LeuXaa: 0.0 ± 0.0
Met
3.079MetAla: 3.079 ± 0.379
0.284MetCys: 0.284 ± 0.114
0.9MetAsp: 0.9 ± 0.212
1.09MetGlu: 1.09 ± 0.264
0.426MetPhe: 0.426 ± 0.131
1.563MetGly: 1.563 ± 0.22
0.379MetHis: 0.379 ± 0.114
0.711MetIle: 0.711 ± 0.177
0.805MetLys: 0.805 ± 0.179
1.895MetLeu: 1.895 ± 0.272
0.474MetMet: 0.474 ± 0.145
0.663MetAsn: 0.663 ± 0.212
1.279MetPro: 1.279 ± 0.264
0.711MetGln: 0.711 ± 0.19
1.374MetArg: 1.374 ± 0.275
2.416MetSer: 2.416 ± 0.32
1.848MetThr: 1.848 ± 0.233
1.137MetVal: 1.137 ± 0.184
0.569MetTrp: 0.569 ± 0.193
0.569MetTyr: 0.569 ± 0.159
0.0MetXaa: 0.0 ± 0.0
Asn
4.548AsnAla: 4.548 ± 0.498
0.284AsnCys: 0.284 ± 0.115
2.321AsnAsp: 2.321 ± 0.405
1.8AsnGlu: 1.8 ± 0.258
0.948AsnPhe: 0.948 ± 0.199
3.743AsnGly: 3.743 ± 0.416
0.758AsnHis: 0.758 ± 0.169
1.563AsnIle: 1.563 ± 0.283
1.563AsnLys: 1.563 ± 0.28
3.269AsnLeu: 3.269 ± 0.391
0.616AsnMet: 0.616 ± 0.165
1.658AsnAsn: 1.658 ± 0.318
2.795AsnPro: 2.795 ± 0.366
1.232AsnGln: 1.232 ± 0.225
2.274AsnArg: 2.274 ± 0.295
1.469AsnSer: 1.469 ± 0.25
1.942AsnThr: 1.942 ± 0.354
2.416AsnVal: 2.416 ± 0.306
0.616AsnTrp: 0.616 ± 0.146
0.853AsnTyr: 0.853 ± 0.214
0.0AsnXaa: 0.0 ± 0.0
Pro
4.501ProAla: 4.501 ± 0.631
0.379ProCys: 0.379 ± 0.125
3.837ProAsp: 3.837 ± 0.521
4.122ProGlu: 4.122 ± 0.435
1.516ProPhe: 1.516 ± 0.309
4.974ProGly: 4.974 ± 0.987
0.853ProHis: 0.853 ± 0.192
1.706ProIle: 1.706 ± 0.259
2.558ProLys: 2.558 ± 0.48
3.269ProLeu: 3.269 ± 0.512
1.184ProMet: 1.184 ± 0.224
2.464ProAsn: 2.464 ± 0.387
2.795ProPro: 2.795 ± 0.392
1.895ProGln: 1.895 ± 0.289
2.369ProArg: 2.369 ± 0.316
2.843ProSer: 2.843 ± 0.322
3.411ProThr: 3.411 ± 0.472
3.695ProVal: 3.695 ± 0.443
1.374ProTrp: 1.374 ± 0.265
1.137ProTyr: 1.137 ± 0.248
0.0ProXaa: 0.0 ± 0.0
Gln
4.216GlnAla: 4.216 ± 0.531
0.474GlnCys: 0.474 ± 0.169
1.706GlnAsp: 1.706 ± 0.28
1.611GlnGlu: 1.611 ± 0.262
1.563GlnPhe: 1.563 ± 0.288
2.464GlnGly: 2.464 ± 0.283
0.521GlnHis: 0.521 ± 0.193
2.227GlnIle: 2.227 ± 0.28
1.563GlnLys: 1.563 ± 0.305
2.843GlnLeu: 2.843 ± 0.379
0.663GlnMet: 0.663 ± 0.197
1.232GlnAsn: 1.232 ± 0.227
1.895GlnPro: 1.895 ± 0.296
1.516GlnGln: 1.516 ± 0.424
3.079GlnArg: 3.079 ± 0.293
1.469GlnSer: 1.469 ± 0.244
1.753GlnThr: 1.753 ± 0.288
3.553GlnVal: 3.553 ± 0.378
0.616GlnTrp: 0.616 ± 0.18
0.948GlnTyr: 0.948 ± 0.218
0.0GlnXaa: 0.0 ± 0.0
Arg
7.58ArgAla: 7.58 ± 0.607
0.853ArgCys: 0.853 ± 0.271
4.264ArgAsp: 4.264 ± 0.461
4.974ArgGlu: 4.974 ± 0.481
1.848ArgPhe: 1.848 ± 0.275
5.401ArgGly: 5.401 ± 0.515
1.184ArgHis: 1.184 ± 0.221
2.416ArgIle: 2.416 ± 0.328
4.311ArgLys: 4.311 ± 0.561
4.88ArgLeu: 4.88 ± 0.463
1.232ArgMet: 1.232 ± 0.29
2.416ArgAsn: 2.416 ± 0.355
2.227ArgPro: 2.227 ± 0.347
2.227ArgGln: 2.227 ± 0.258
3.98ArgArg: 3.98 ± 0.437
3.316ArgSer: 3.316 ± 0.367
3.222ArgThr: 3.222 ± 0.516
4.359ArgVal: 4.359 ± 0.394
1.516ArgTrp: 1.516 ± 0.332
1.753ArgTyr: 1.753 ± 0.285
0.0ArgXaa: 0.0 ± 0.0
Ser
6.017SerAla: 6.017 ± 0.573
0.284SerCys: 0.284 ± 0.132
3.411SerAsp: 3.411 ± 0.417
3.269SerGlu: 3.269 ± 0.348
1.516SerPhe: 1.516 ± 0.33
6.633SerGly: 6.633 ± 0.927
1.09SerHis: 1.09 ± 0.222
2.511SerIle: 2.511 ± 0.334
2.937SerLys: 2.937 ± 0.301
4.453SerLeu: 4.453 ± 0.425
1.374SerMet: 1.374 ± 0.224
1.658SerAsn: 1.658 ± 0.216
2.464SerPro: 2.464 ± 0.334
1.611SerGln: 1.611 ± 0.264
3.127SerArg: 3.127 ± 0.436
2.937SerSer: 2.937 ± 0.408
2.937SerThr: 2.937 ± 0.422
4.169SerVal: 4.169 ± 0.404
1.374SerTrp: 1.374 ± 0.238
1.611SerTyr: 1.611 ± 0.269
0.0SerXaa: 0.0 ± 0.0
Thr
6.443ThrAla: 6.443 ± 0.84
0.284ThrCys: 0.284 ± 0.115
3.601ThrAsp: 3.601 ± 0.49
2.843ThrGlu: 2.843 ± 0.399
2.179ThrPhe: 2.179 ± 0.339
5.543ThrGly: 5.543 ± 0.637
0.995ThrHis: 0.995 ± 0.213
2.653ThrIle: 2.653 ± 0.47
1.942ThrLys: 1.942 ± 0.363
5.448ThrLeu: 5.448 ± 0.498
1.09ThrMet: 1.09 ± 0.231
2.037ThrAsn: 2.037 ± 0.35
3.269ThrPro: 3.269 ± 0.366
1.942ThrGln: 1.942 ± 0.33
3.127ThrArg: 3.127 ± 0.382
2.937ThrSer: 2.937 ± 0.352
3.506ThrThr: 3.506 ± 0.46
5.685ThrVal: 5.685 ± 0.723
1.137ThrTrp: 1.137 ± 0.278
2.037ThrTyr: 2.037 ± 0.31
0.0ThrXaa: 0.0 ± 0.0
Val
7.438ValAla: 7.438 ± 0.836
0.995ValCys: 0.995 ± 0.217
5.022ValAsp: 5.022 ± 0.509
3.601ValGlu: 3.601 ± 0.402
1.706ValPhe: 1.706 ± 0.33
5.448ValGly: 5.448 ± 0.518
1.374ValHis: 1.374 ± 0.253
3.032ValIle: 3.032 ± 0.376
3.506ValLys: 3.506 ± 0.448
5.401ValLeu: 5.401 ± 0.539
1.516ValMet: 1.516 ± 0.248
2.274ValAsn: 2.274 ± 0.367
4.027ValPro: 4.027 ± 0.529
2.937ValGln: 2.937 ± 0.377
4.643ValArg: 4.643 ± 0.451
4.88ValSer: 4.88 ± 0.518
5.306ValThr: 5.306 ± 0.786
6.017ValVal: 6.017 ± 0.988
1.327ValTrp: 1.327 ± 0.249
1.99ValTyr: 1.99 ± 0.313
0.0ValXaa: 0.0 ± 0.0
Trp
1.99TrpAla: 1.99 ± 0.266
0.142TrpCys: 0.142 ± 0.081
1.658TrpAsp: 1.658 ± 0.332
0.9TrpGlu: 0.9 ± 0.227
0.332TrpPhe: 0.332 ± 0.129
1.279TrpGly: 1.279 ± 0.199
0.758TrpHis: 0.758 ± 0.206
0.9TrpIle: 0.9 ± 0.215
0.711TrpLys: 0.711 ± 0.193
2.511TrpLeu: 2.511 ± 0.337
0.569TrpMet: 0.569 ± 0.152
1.09TrpAsn: 1.09 ± 0.235
0.995TrpPro: 0.995 ± 0.227
0.9TrpGln: 0.9 ± 0.19
1.469TrpArg: 1.469 ± 0.281
1.137TrpSer: 1.137 ± 0.279
1.421TrpThr: 1.421 ± 0.307
1.232TrpVal: 1.232 ± 0.211
0.569TrpTrp: 0.569 ± 0.196
0.284TrpTyr: 0.284 ± 0.138
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.843TyrAla: 2.843 ± 0.413
0.332TyrCys: 0.332 ± 0.141
2.037TyrAsp: 2.037 ± 0.301
1.848TyrGlu: 1.848 ± 0.325
1.232TyrPhe: 1.232 ± 0.242
3.079TyrGly: 3.079 ± 0.321
0.332TyrHis: 0.332 ± 0.133
0.711TyrIle: 0.711 ± 0.169
1.09TyrLys: 1.09 ± 0.21
2.274TyrLeu: 2.274 ± 0.273
0.569TyrMet: 0.569 ± 0.164
0.995TyrAsn: 0.995 ± 0.212
1.279TyrPro: 1.279 ± 0.197
1.232TyrGln: 1.232 ± 0.244
2.037TyrArg: 2.037 ± 0.254
1.563TyrSer: 1.563 ± 0.344
1.611TyrThr: 1.611 ± 0.303
1.895TyrVal: 1.895 ± 0.267
0.663TyrTrp: 0.663 ± 0.192
0.711TyrTyr: 0.711 ± 0.178
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 110 proteins (21109 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski