Malus baccata (Siberian crab apple) (Pyrus baccata)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Maleae; Malus

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45510 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A540MQV9|A0A540MQV9_MALBA Uncharacterized protein OS=Malus baccata OX=106549 GN=C1H46_013248 PE=4 SV=1
MM1 pKa = 7.35IVASNSKK8 pKa = 9.79ARR10 pKa = 11.84KK11 pKa = 8.17IMYY14 pKa = 9.83YY15 pKa = 10.94LLAQKK20 pKa = 10.11IFYY23 pKa = 10.23SYY25 pKa = 11.12EE26 pKa = 3.76QMHH29 pKa = 6.83GDD31 pKa = 4.73DD32 pKa = 5.25YY33 pKa = 12.0DD34 pKa = 6.02GDD36 pKa = 4.63DD37 pKa = 4.47YY38 pKa = 11.96NDD40 pKa = 3.81SDD42 pKa = 3.97SSSVLEE48 pKa = 4.55ADD50 pKa = 5.09EE51 pKa = 5.3FDD53 pKa = 4.16HH54 pKa = 7.56DD55 pKa = 4.25QDD57 pKa = 4.63KK58 pKa = 11.62DD59 pKa = 3.61DD60 pKa = 4.95AEE62 pKa = 4.58RR63 pKa = 11.84KK64 pKa = 9.07LKK66 pKa = 10.84YY67 pKa = 10.38YY68 pKa = 11.11LLAQKK73 pKa = 9.49MFYY76 pKa = 10.32LYY78 pKa = 10.46EE79 pKa = 3.93QTEE82 pKa = 4.29DD83 pKa = 3.44GDD85 pKa = 4.06YY86 pKa = 11.48DD87 pKa = 5.11GDD89 pKa = 5.07DD90 pKa = 4.77DD91 pKa = 6.58DD92 pKa = 6.42SDD94 pKa = 4.14SSSVLEE100 pKa = 5.18DD101 pKa = 3.6DD102 pKa = 5.74QFDD105 pKa = 3.77HH106 pKa = 7.34DD107 pKa = 3.96QDD109 pKa = 3.95KK110 pKa = 11.37DD111 pKa = 3.77SSS113 pKa = 3.69

Molecular weight: 13.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A540N9F0|A0A540N9F0_MALBA SAM domain-containing protein OS=Malus baccata OX=106549 GN=C1H46_006609 PE=4 SV=1
MM1 pKa = 7.56GKK3 pKa = 10.49GPFSFKK9 pKa = 9.94RR10 pKa = 11.84SSSIRR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84PKK19 pKa = 10.47KK20 pKa = 10.44FIGPPPSPFPSPPQPIRR37 pKa = 11.84SPPPSNNVNINNTNTNNNNNSLSAVVAGGGLTALSFRR74 pKa = 11.84FWRR77 pKa = 11.84LHH79 pKa = 4.86

Molecular weight: 8.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45510

0

45510

16148503

49

6024

354.8

39.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.795 ± 0.012

1.864 ± 0.006

5.274 ± 0.008

6.437 ± 0.013

4.162 ± 0.007

6.67 ± 0.012

2.421 ± 0.006

5.081 ± 0.009

6.066 ± 0.011

9.676 ± 0.014

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.426 ± 0.005

4.387 ± 0.007

5.051 ± 0.013

3.719 ± 0.008

5.326 ± 0.009

8.978 ± 0.016

4.938 ± 0.008

6.62 ± 0.008

1.29 ± 0.004

2.74 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski