Psychroflexus sediminis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Psychroflexus

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2655 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G7XD03|A0A1G7XD03_9FLAO mRNA interferase HigB OS=Psychroflexus sediminis OX=470826 GN=SAMN04488027_10833 PE=4 SV=1
MM1 pKa = 6.91KK2 pKa = 10.22QIYY5 pKa = 9.91SLLILFIVSLSYY17 pKa = 11.04GQVINPGTTSANIVSNSAPNITCPEE42 pKa = 4.58EE43 pKa = 3.83ITTTATSPIVNFNNPTLTDD62 pKa = 3.5NEE64 pKa = 4.27TTAFPFVSSDD74 pKa = 3.05MTYY77 pKa = 10.15IGSNGGKK84 pKa = 9.57NFFISEE90 pKa = 4.05QSFSGANAFADD101 pKa = 5.0AIARR105 pKa = 11.84GGLVATISDD114 pKa = 3.54ATQNTFIANALQANNITEE132 pKa = 3.98AHH134 pKa = 6.32IGYY137 pKa = 9.75SDD139 pKa = 3.74AASEE143 pKa = 5.24GNFVWQDD150 pKa = 3.09GSTSGYY156 pKa = 10.16EE157 pKa = 3.48NWQPNEE163 pKa = 4.09PNDD166 pKa = 3.69TDD168 pKa = 3.59GGEE171 pKa = 4.35DD172 pKa = 4.04YY173 pKa = 10.67VTISAGGGWNDD184 pKa = 2.87VGARR188 pKa = 11.84GTGNARR194 pKa = 11.84YY195 pKa = 9.32IFQLDD200 pKa = 3.39QTDD203 pKa = 3.43GFLIQTSGLASGSSFPIGTTTNTFEE228 pKa = 5.75AYY230 pKa = 9.23DD231 pKa = 3.5ASGNSSICSFNVTVTDD247 pKa = 3.24IPEE250 pKa = 4.13VVTQNIDD257 pKa = 3.32VVLDD261 pKa = 3.41ATGNATISPEE271 pKa = 4.14EE272 pKa = 4.03IDD274 pKa = 3.64NGSTAVSGILGLSLDD289 pKa = 4.48ILNFDD294 pKa = 4.63CSNLGEE300 pKa = 4.26NTVTLTVISNTVEE313 pKa = 4.2TATGTAIVTVRR324 pKa = 11.84DD325 pKa = 3.76TTAPTVVTQDD335 pKa = 2.82ITVEE339 pKa = 3.96LDD341 pKa = 3.05ASGEE345 pKa = 4.17AIITADD351 pKa = 4.04QIDD354 pKa = 4.31NGSADD359 pKa = 3.42NCGIASLDD367 pKa = 3.58LSRR370 pKa = 11.84TNFNGFDD377 pKa = 3.01IGQNTVEE384 pKa = 4.59LKK386 pKa = 9.63VTDD389 pKa = 3.49SSGNQSAGTATVTIVDD405 pKa = 4.1TTPPNAVCSNQTIFLDD421 pKa = 3.75EE422 pKa = 4.75SGNASISASDD432 pKa = 3.59VDD434 pKa = 5.41GGSSDD439 pKa = 3.71NASAAYY445 pKa = 9.39IPLLNYY451 pKa = 10.11EE452 pKa = 4.61PIEE455 pKa = 4.36TGSGINASEE464 pKa = 4.42FEE466 pKa = 4.26FVAQVVTIPQPISDD480 pKa = 3.88FRR482 pKa = 11.84IKK484 pKa = 10.96LEE486 pKa = 4.29LATCKK491 pKa = 10.91SNGLTGATLDD501 pKa = 3.85IVEE504 pKa = 5.53LDD506 pKa = 3.89PSGHH510 pKa = 7.1PDD512 pKa = 3.13LSLVLGSSSVPEE524 pKa = 3.7SDD526 pKa = 2.84IYY528 pKa = 11.15GWPGCVSGSFGLTTFDD544 pKa = 4.1FSGVDD549 pKa = 3.6LQQGNQYY556 pKa = 11.66ALVLKK561 pKa = 10.32NSEE564 pKa = 4.11GARR567 pKa = 11.84GSVSWNRR574 pKa = 11.84SAIPTDD580 pKa = 3.48PSNPYY585 pKa = 9.82QGGNIWTYY593 pKa = 8.8EE594 pKa = 4.02NYY596 pKa = 10.11RR597 pKa = 11.84SRR599 pKa = 11.84LLSDD603 pKa = 3.44EE604 pKa = 4.12VWRR607 pKa = 11.84EE608 pKa = 4.0DD609 pKa = 3.59EE610 pKa = 4.11THH612 pKa = 7.14DD613 pKa = 3.52LHH615 pKa = 8.5FSIEE619 pKa = 3.99ALQEE623 pKa = 4.16DD624 pKa = 4.74LTYY627 pKa = 10.92SIDD630 pKa = 3.74QDD632 pKa = 4.74SFDD635 pKa = 4.28CSNLGVNTVSLTVTDD650 pKa = 4.11ASGNTSSCISEE661 pKa = 3.97VTVIDD666 pKa = 3.64NLAPTVVTQDD676 pKa = 2.82ITVEE680 pKa = 3.79LDD682 pKa = 3.21ANGEE686 pKa = 4.07AGITADD692 pKa = 4.51QIDD695 pKa = 4.45NGTSDD700 pKa = 3.67NCSSISGYY708 pKa = 10.31CIEE711 pKa = 4.6GMTYY715 pKa = 10.71GNAEE719 pKa = 3.93IFIEE723 pKa = 4.56GAGMGRR729 pKa = 11.84VDD731 pKa = 4.84FSAGLVSASSGGVPMEE747 pKa = 4.5LVPITTIDD755 pKa = 3.46SKK757 pKa = 11.41VRR759 pKa = 11.84SGDD762 pKa = 3.46LVHH765 pKa = 7.17ARR767 pKa = 11.84STLSTGSRR775 pKa = 11.84PSGEE779 pKa = 4.24SIFYY783 pKa = 10.0RR784 pKa = 11.84VSDD787 pKa = 3.99GRR789 pKa = 11.84IIWGGHH795 pKa = 4.58SPSGSFANRR804 pKa = 11.84TQLVIEE810 pKa = 4.3QPNVATGEE818 pKa = 4.46LIDD821 pKa = 4.55FSKK824 pKa = 10.68PYY826 pKa = 10.3NLKK829 pKa = 9.25FQLNGRR835 pKa = 11.84YY836 pKa = 9.12LSQSGGSFTSVSSPADD852 pKa = 3.06ISAVLISGDD861 pKa = 3.7LGATNNCLLTTSLDD875 pKa = 3.34ATTFDD880 pKa = 5.04CSNLGDD886 pKa = 3.91NEE888 pKa = 4.18VTLTVTDD895 pKa = 4.41GSGNSSSATAIVTVIDD911 pKa = 4.51NIAPTIVLKK920 pKa = 10.96DD921 pKa = 3.33VTVEE925 pKa = 3.89LNAEE929 pKa = 4.14GTVSVEE935 pKa = 4.59ASAFDD940 pKa = 3.53NGSFDD945 pKa = 3.41NCGEE949 pKa = 4.31VTFSTNLVEE958 pKa = 5.04ILSCGALGLNEE969 pKa = 4.07VTVTVTDD976 pKa = 3.69ASGNQSQNTAILTVLDD992 pKa = 4.08NLSPAVVTQDD1002 pKa = 3.11FTVVLNANGEE1012 pKa = 4.21ATITAAQVDD1021 pKa = 4.2NGSADD1026 pKa = 3.33NCGVDD1031 pKa = 4.04TLALDD1036 pKa = 3.5ITTFDD1041 pKa = 4.27CSNLGEE1047 pKa = 4.23NTVTLTATDD1056 pKa = 4.1GSGKK1060 pKa = 9.72QATATAIVTVVDD1072 pKa = 3.65NSLPTAVAQNLTVEE1086 pKa = 4.18LDD1088 pKa = 3.53EE1089 pKa = 5.56FGAASITAAQVDD1101 pKa = 4.03NGSSDD1106 pKa = 3.33NCGVEE1111 pKa = 4.31TLALDD1116 pKa = 3.77VTSFDD1121 pKa = 4.49CSNLGEE1127 pKa = 4.3NTVTLRR1133 pKa = 11.84VTDD1136 pKa = 4.12GSGNQATSTAVITVIDD1152 pKa = 4.21ALAPALVTADD1162 pKa = 3.13ITVSLDD1168 pKa = 3.21EE1169 pKa = 4.52NATVSIEE1176 pKa = 3.91PGNLVDD1182 pKa = 4.64TLEE1185 pKa = 5.03DD1186 pKa = 3.52NCSLAGEE1193 pKa = 4.25ITLALDD1199 pKa = 3.17QDD1201 pKa = 4.2TFTATGIYY1209 pKa = 9.25VVNITATDD1217 pKa = 3.41ASGNTTTEE1225 pKa = 4.17SAEE1228 pKa = 4.08VTVDD1232 pKa = 3.2STLSIDD1238 pKa = 4.4QVDD1241 pKa = 3.46SDD1243 pKa = 4.5IQVKK1247 pKa = 10.01LYY1249 pKa = 9.6PNPTTDD1255 pKa = 3.02HH1256 pKa = 6.76LFFEE1260 pKa = 4.9VSNTEE1265 pKa = 4.45LIHH1268 pKa = 5.15ITIYY1272 pKa = 11.35DD1273 pKa = 3.72MNGKK1277 pKa = 8.77LVKK1280 pKa = 9.85TSQEE1284 pKa = 3.94SHH1286 pKa = 6.22SVDD1289 pKa = 3.36VTNLSSGVYY1298 pKa = 8.62FAKK1301 pKa = 10.51VDD1303 pKa = 3.66AKK1305 pKa = 11.0GGNTLKK1311 pKa = 10.57ILRR1314 pKa = 11.84FIKK1317 pKa = 10.44KK1318 pKa = 9.73

Molecular weight: 137.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G7XSZ8|A0A1G7XSZ8_9FLAO Peptide methionine sulfoxide reductase MsrA OS=Psychroflexus sediminis OX=470826 GN=msrA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNKK10 pKa = 8.92KK11 pKa = 9.95RR12 pKa = 11.84KK13 pKa = 8.04NKK15 pKa = 9.86HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.19RR21 pKa = 11.84MSSVSGRR28 pKa = 11.84KK29 pKa = 7.69VLKK32 pKa = 10.02RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.65LSVSSEE47 pKa = 3.37MSLRR51 pKa = 11.84KK52 pKa = 9.51RR53 pKa = 3.5

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2655

0

2655

886846

40

2412

334.0

37.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.252 ± 0.042

0.643 ± 0.013

5.76 ± 0.035

7.271 ± 0.043

5.414 ± 0.039

6.281 ± 0.044

1.851 ± 0.023

7.59 ± 0.047

7.56 ± 0.058

9.719 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.193 ± 0.023

5.458 ± 0.051

3.407 ± 0.027

3.639 ± 0.027

3.656 ± 0.031

6.959 ± 0.042

5.343 ± 0.039

6.086 ± 0.038

1.016 ± 0.017

3.901 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski