Mariprofundus ferrooxydans PV-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Zetaproteobacteria; Mariprofundales; Mariprofundaceae; Mariprofundus; Mariprofundus ferrooxydans

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2856 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q0F212|Q0F212_9PROT RNA-binding region RNP-1 (RNA recognition motif) OS=Mariprofundus ferrooxydans PV-1 OX=314345 GN=SPV1_02282 PE=4 SV=1
MM1 pKa = 7.31QICNHH6 pKa = 6.1KK7 pKa = 10.42VVSLQFSMSNDD18 pKa = 3.15AGEE21 pKa = 4.58VLDD24 pKa = 5.58SSDD27 pKa = 5.41DD28 pKa = 3.86GDD30 pKa = 3.85ALLYY34 pKa = 10.75LHH36 pKa = 6.96GEE38 pKa = 4.22EE39 pKa = 4.8NIVPGLEE46 pKa = 3.79LALEE50 pKa = 4.59GKK52 pKa = 10.01SVGDD56 pKa = 3.64KK57 pKa = 11.24LKK59 pKa = 9.85VTLDD63 pKa = 3.15AADD66 pKa = 4.13AYY68 pKa = 11.03GEE70 pKa = 4.13IDD72 pKa = 3.52PSLVEE77 pKa = 4.16VVSAEE82 pKa = 3.95MFEE85 pKa = 4.32GVDD88 pKa = 3.6NIEE91 pKa = 3.88VGMEE95 pKa = 4.37FEE97 pKa = 5.93AEE99 pKa = 4.17MPDD102 pKa = 3.88EE103 pKa = 6.25DD104 pKa = 3.68EE105 pKa = 4.24LQYY108 pKa = 11.57VRR110 pKa = 11.84ITEE113 pKa = 3.81IDD115 pKa = 3.54GDD117 pKa = 4.07NVTVDD122 pKa = 3.91GNHH125 pKa = 7.07PLAGMRR131 pKa = 11.84LHH133 pKa = 7.16FDD135 pKa = 3.35VTVEE139 pKa = 4.86AIRR142 pKa = 11.84EE143 pKa = 4.25ATEE146 pKa = 3.71EE147 pKa = 4.08EE148 pKa = 4.75LEE150 pKa = 4.41HH151 pKa = 6.66GHH153 pKa = 5.03VHH155 pKa = 7.07GGEE158 pKa = 4.32CCGEE162 pKa = 3.83EE163 pKa = 4.31DD164 pKa = 4.73CCNN167 pKa = 3.45

Molecular weight: 18.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q0EVP3|Q0EVP3_9PROT AAA domain-containing protein (Fragment) OS=Mariprofundus ferrooxydans PV-1 OX=314345 GN=SPV1_14424 PE=4 SV=1
II1 pKa = 6.66SQPHH5 pKa = 5.21VRR7 pKa = 11.84PIVRR11 pKa = 11.84GKK13 pKa = 10.32AGRR16 pKa = 11.84PVEE19 pKa = 4.8FGAKK23 pKa = 9.72VSVSMIGKK31 pKa = 9.17LAFVDD36 pKa = 3.85HH37 pKa = 7.09LDD39 pKa = 3.04WDD41 pKa = 4.37AFNEE45 pKa = 4.32SQDD48 pKa = 4.76LIAQVEE54 pKa = 4.55RR55 pKa = 11.84YY56 pKa = 8.34KK57 pKa = 11.17ARR59 pKa = 11.84FGFYY63 pKa = 10.19PEE65 pKa = 4.3VVLADD70 pKa = 4.53GIYY73 pKa = 8.65GTRR76 pKa = 11.84ANRR79 pKa = 11.84RR80 pKa = 11.84WLKK83 pKa = 9.22EE84 pKa = 3.32HH85 pKa = 7.38GIRR88 pKa = 11.84FGGRR92 pKa = 11.84PLGRR96 pKa = 11.84PRR98 pKa = 11.84KK99 pKa = 8.29VTSEE103 pKa = 3.7NMDD106 pKa = 3.82EE107 pKa = 4.08IKK109 pKa = 10.33QLKK112 pKa = 7.81RR113 pKa = 11.84QRR115 pKa = 11.84RR116 pKa = 11.84EE117 pKa = 3.93DD118 pKa = 3.48EE119 pKa = 3.91RR120 pKa = 11.84SRR122 pKa = 11.84IPVEE126 pKa = 4.11GKK128 pKa = 10.21FGQGKK133 pKa = 9.3NGYY136 pKa = 8.9RR137 pKa = 11.84LNQIRR142 pKa = 11.84ARR144 pKa = 11.84LASTSEE150 pKa = 3.41AWVRR154 pKa = 11.84SIFLVMNLIALLRR167 pKa = 11.84FLLPFLRR174 pKa = 11.84PSAIMDD180 pKa = 4.09ACTTWLRR187 pKa = 11.84GMLRR191 pKa = 11.84LDD193 pKa = 4.27DD194 pKa = 4.45GVGDD198 pKa = 4.63RR199 pKa = 11.84LTGMHH204 pKa = 6.99PRR206 pKa = 11.84FADD209 pKa = 4.18ASTGWW214 pKa = 3.15

Molecular weight: 24.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2856

0

2856

856874

20

1592

300.0

33.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.402 ± 0.065

1.146 ± 0.016

5.713 ± 0.034

5.932 ± 0.04

3.63 ± 0.029

7.591 ± 0.044

2.698 ± 0.026

5.917 ± 0.039

4.105 ± 0.042

10.522 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.105 ± 0.025

3.083 ± 0.028

4.321 ± 0.029

4.012 ± 0.039

6.099 ± 0.037

5.997 ± 0.034

4.863 ± 0.028

6.918 ± 0.045

1.352 ± 0.019

2.593 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski