Escherichia phage VEc3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Molineuxvirinae; Vectrevirus; Escherichia virus VEc3

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H4YDF6|A0A2H4YDF6_9CAUD Uncharacterized protein OS=Escherichia phage VEc3 OX=2783801 GN=vec3_07 PE=4 SV=1
MM1 pKa = 7.48AMSNMTYY8 pKa = 9.84SQVYY12 pKa = 7.34NTAHH16 pKa = 6.61AALVEE21 pKa = 4.35RR22 pKa = 11.84VWDD25 pKa = 4.14DD26 pKa = 5.48AIDD29 pKa = 4.95CIDD32 pKa = 3.8NAHH35 pKa = 6.71DD36 pKa = 5.38AIHH39 pKa = 6.31EE40 pKa = 4.45VADD43 pKa = 3.79SAVPIYY49 pKa = 10.46YY50 pKa = 10.45ADD52 pKa = 3.63IFSVMASEE60 pKa = 5.34GIDD63 pKa = 3.4HH64 pKa = 6.92FFEE67 pKa = 6.26DD68 pKa = 4.36SGLMPDD74 pKa = 3.68TKK76 pKa = 10.96DD77 pKa = 3.3VTVVLQARR85 pKa = 11.84IYY87 pKa = 9.25EE88 pKa = 4.14QLTIDD93 pKa = 3.14LWEE96 pKa = 4.59RR97 pKa = 11.84VQDD100 pKa = 4.14MIDD103 pKa = 3.46AYY105 pKa = 9.93IDD107 pKa = 3.48SAEE110 pKa = 4.12DD111 pKa = 3.45SEE113 pKa = 4.75EE114 pKa = 4.12EE115 pKa = 4.17EE116 pKa = 4.44EE117 pKa = 4.34

Molecular weight: 13.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H4YE69|A0A2H4YE69_9CAUD Tail tubular protein A OS=Escherichia phage VEc3 OX=2783801 GN=vec3_38 PE=4 SV=1
MM1 pKa = 7.3RR2 pKa = 11.84TNFEE6 pKa = 3.89KK7 pKa = 8.45MTRR10 pKa = 11.84KK11 pKa = 9.93ANRR14 pKa = 11.84FDD16 pKa = 3.58MEE18 pKa = 4.24EE19 pKa = 4.14GQKK22 pKa = 10.19KK23 pKa = 9.6GKK25 pKa = 9.25KK26 pKa = 9.82LNVPVRR32 pKa = 11.84DD33 pKa = 3.64RR34 pKa = 11.84ASKK37 pKa = 8.92RR38 pKa = 11.84AAWEE42 pKa = 3.95FF43 pKa = 3.49

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

57

0

57

13735

32

1102

241.0

26.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.933 ± 0.401

1.099 ± 0.173

6.036 ± 0.22

7.15 ± 0.324

3.757 ± 0.15

7.667 ± 0.304

2.039 ± 0.222

5.351 ± 0.25

6.844 ± 0.292

7.87 ± 0.222

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.087 ± 0.172

4.084 ± 0.179

3.451 ± 0.176

4.223 ± 0.284

5.315 ± 0.282

6.087 ± 0.395

5.446 ± 0.238

6.72 ± 0.252

1.391 ± 0.135

3.451 ± 0.157
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski