Amino acid dipepetide frequency for Escherichia phage VEc3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.082AlaAla: 8.082 ± 1.218
0.582AlaCys: 0.582 ± 0.229
5.534AlaAsp: 5.534 ± 0.548
6.116AlaGlu: 6.116 ± 0.718
3.204AlaPhe: 3.204 ± 0.58
6.699AlaGly: 6.699 ± 0.892
1.602AlaHis: 1.602 ± 0.326
4.951AlaIle: 4.951 ± 0.506
5.388AlaLys: 5.388 ± 0.465
7.281AlaLeu: 7.281 ± 0.809
3.131AlaMet: 3.131 ± 0.448
3.349AlaAsn: 3.349 ± 0.467
2.985AlaPro: 2.985 ± 0.471
4.878AlaGln: 4.878 ± 0.757
5.315AlaArg: 5.315 ± 0.688
5.461AlaSer: 5.461 ± 0.709
4.442AlaThr: 4.442 ± 0.559
6.553AlaVal: 6.553 ± 0.747
0.874AlaTrp: 0.874 ± 0.27
2.912AlaTyr: 2.912 ± 0.408
0.0AlaXaa: 0.0 ± 0.0
Cys
0.437CysAla: 0.437 ± 0.166
0.073CysCys: 0.073 ± 0.075
0.874CysAsp: 0.874 ± 0.331
0.437CysGlu: 0.437 ± 0.219
0.728CysPhe: 0.728 ± 0.214
0.874CysGly: 0.874 ± 0.31
0.291CysHis: 0.291 ± 0.19
0.655CysIle: 0.655 ± 0.262
0.801CysLys: 0.801 ± 0.268
0.874CysLeu: 0.874 ± 0.279
0.364CysMet: 0.364 ± 0.172
0.582CysAsn: 0.582 ± 0.232
0.51CysPro: 0.51 ± 0.223
0.582CysGln: 0.582 ± 0.243
0.655CysArg: 0.655 ± 0.26
0.364CysSer: 0.364 ± 0.15
0.364CysThr: 0.364 ± 0.16
0.582CysVal: 0.582 ± 0.201
0.146CysTrp: 0.146 ± 0.1
0.801CysTyr: 0.801 ± 0.275
0.0CysXaa: 0.0 ± 0.0
Asp
6.699AspAla: 6.699 ± 0.895
0.801AspCys: 0.801 ± 0.249
3.277AspAsp: 3.277 ± 0.67
4.442AspGlu: 4.442 ± 0.627
2.039AspPhe: 2.039 ± 0.502
4.66AspGly: 4.66 ± 0.714
0.947AspHis: 0.947 ± 0.282
4.15AspIle: 4.15 ± 0.502
4.077AspLys: 4.077 ± 0.538
4.587AspLeu: 4.587 ± 0.526
2.184AspMet: 2.184 ± 0.402
2.476AspAsn: 2.476 ± 0.395
1.966AspPro: 1.966 ± 0.384
1.019AspGln: 1.019 ± 0.272
2.694AspArg: 2.694 ± 0.374
4.369AspSer: 4.369 ± 0.655
3.204AspThr: 3.204 ± 0.467
3.859AspVal: 3.859 ± 0.522
1.019AspTrp: 1.019 ± 0.352
1.893AspTyr: 1.893 ± 0.417
0.0AspXaa: 0.0 ± 0.0
Glu
7.063GluAla: 7.063 ± 0.772
0.801GluCys: 0.801 ± 0.289
4.806GluAsp: 4.806 ± 0.621
7.572GluGlu: 7.572 ± 0.895
2.33GluPhe: 2.33 ± 0.326
5.461GluGly: 5.461 ± 0.59
1.383GluHis: 1.383 ± 0.402
2.548GluIle: 2.548 ± 0.38
4.878GluLys: 4.878 ± 0.538
6.699GluLeu: 6.699 ± 0.711
2.112GluMet: 2.112 ± 0.466
1.383GluAsn: 1.383 ± 0.309
1.82GluPro: 1.82 ± 0.418
3.277GluGln: 3.277 ± 0.448
3.349GluArg: 3.349 ± 0.487
3.713GluSer: 3.713 ± 0.568
2.621GluThr: 2.621 ± 0.434
5.971GluVal: 5.971 ± 0.615
1.747GluTrp: 1.747 ± 0.293
1.966GluTyr: 1.966 ± 0.334
0.0GluXaa: 0.0 ± 0.0
Phe
2.33PheAla: 2.33 ± 0.405
0.291PheCys: 0.291 ± 0.153
3.204PheAsp: 3.204 ± 0.491
2.84PheGlu: 2.84 ± 0.453
1.238PhePhe: 1.238 ± 0.303
2.767PheGly: 2.767 ± 0.564
0.655PheHis: 0.655 ± 0.212
2.33PheIle: 2.33 ± 0.319
3.204PheLys: 3.204 ± 0.498
3.131PheLeu: 3.131 ± 0.499
1.893PheMet: 1.893 ± 0.351
1.747PheAsn: 1.747 ± 0.41
1.019PhePro: 1.019 ± 0.266
1.747PheGln: 1.747 ± 0.34
1.747PheArg: 1.747 ± 0.349
2.767PheSer: 2.767 ± 0.563
2.33PheThr: 2.33 ± 0.391
1.165PheVal: 1.165 ± 0.288
0.218PheTrp: 0.218 ± 0.115
0.947PheTyr: 0.947 ± 0.279
0.0PheXaa: 0.0 ± 0.0
Gly
6.844GlyAla: 6.844 ± 0.654
0.947GlyCys: 0.947 ± 0.316
5.242GlyAsp: 5.242 ± 0.664
4.15GlyGlu: 4.15 ± 0.673
3.277GlyPhe: 3.277 ± 0.697
5.534GlyGly: 5.534 ± 0.744
1.82GlyHis: 1.82 ± 0.404
4.806GlyIle: 4.806 ± 0.638
6.043GlyLys: 6.043 ± 0.753
5.242GlyLeu: 5.242 ± 0.907
1.966GlyMet: 1.966 ± 0.393
3.422GlyAsn: 3.422 ± 0.485
0.582GlyPro: 0.582 ± 0.227
3.131GlyGln: 3.131 ± 0.428
3.422GlyArg: 3.422 ± 0.529
5.388GlySer: 5.388 ± 0.998
5.024GlyThr: 5.024 ± 0.658
5.388GlyVal: 5.388 ± 0.62
1.456GlyTrp: 1.456 ± 0.286
2.985GlyTyr: 2.985 ± 0.422
0.0GlyXaa: 0.0 ± 0.0
His
1.238HisAla: 1.238 ± 0.317
0.218HisCys: 0.218 ± 0.154
1.529HisAsp: 1.529 ± 0.309
1.238HisGlu: 1.238 ± 0.311
1.092HisPhe: 1.092 ± 0.227
1.602HisGly: 1.602 ± 0.392
0.291HisHis: 0.291 ± 0.133
1.019HisIle: 1.019 ± 0.257
1.456HisLys: 1.456 ± 0.358
2.33HisLeu: 2.33 ± 0.379
0.437HisMet: 0.437 ± 0.297
0.728HisAsn: 0.728 ± 0.226
0.801HisPro: 0.801 ± 0.256
0.728HisGln: 0.728 ± 0.287
1.311HisArg: 1.311 ± 0.419
0.728HisSer: 0.728 ± 0.196
0.947HisThr: 0.947 ± 0.256
1.383HisVal: 1.383 ± 0.367
0.146HisTrp: 0.146 ± 0.095
1.165HisTyr: 1.165 ± 0.283
0.0HisXaa: 0.0 ± 0.0
Ile
4.878IleAla: 4.878 ± 0.488
0.218IleCys: 0.218 ± 0.139
4.077IleAsp: 4.077 ± 0.671
3.786IleGlu: 3.786 ± 0.596
1.82IlePhe: 1.82 ± 0.503
4.66IleGly: 4.66 ± 0.647
1.092IleHis: 1.092 ± 0.33
3.422IleIle: 3.422 ± 0.656
4.077IleLys: 4.077 ± 0.528
3.349IleLeu: 3.349 ± 0.487
1.529IleMet: 1.529 ± 0.334
1.893IleAsn: 1.893 ± 0.294
2.184IlePro: 2.184 ± 0.35
2.039IleGln: 2.039 ± 0.441
2.84IleArg: 2.84 ± 0.518
2.403IleSer: 2.403 ± 0.435
3.495IleThr: 3.495 ± 0.555
3.495IleVal: 3.495 ± 0.455
0.801IleTrp: 0.801 ± 0.197
1.456IleTyr: 1.456 ± 0.419
0.0IleXaa: 0.0 ± 0.0
Lys
7.864LysAla: 7.864 ± 0.807
0.801LysCys: 0.801 ± 0.246
3.058LysAsp: 3.058 ± 0.54
5.17LysGlu: 5.17 ± 0.672
1.747LysPhe: 1.747 ± 0.399
6.116LysGly: 6.116 ± 0.681
1.82LysHis: 1.82 ± 0.298
3.349LysIle: 3.349 ± 0.404
5.242LysLys: 5.242 ± 0.778
5.534LysLeu: 5.534 ± 0.653
1.383LysMet: 1.383 ± 0.305
2.184LysAsn: 2.184 ± 0.417
3.204LysPro: 3.204 ± 0.387
2.548LysGln: 2.548 ± 0.478
3.859LysArg: 3.859 ± 0.435
3.932LysSer: 3.932 ± 0.475
2.621LysThr: 2.621 ± 0.389
4.514LysVal: 4.514 ± 0.863
0.801LysTrp: 0.801 ± 0.227
2.694LysTyr: 2.694 ± 0.38
0.0LysXaa: 0.0 ± 0.0
Leu
6.335LeuAla: 6.335 ± 0.658
0.947LeuCys: 0.947 ± 0.281
5.607LeuAsp: 5.607 ± 0.531
5.17LeuGlu: 5.17 ± 0.628
3.058LeuPhe: 3.058 ± 0.521
5.679LeuGly: 5.679 ± 1.059
1.238LeuHis: 1.238 ± 0.363
4.223LeuIle: 4.223 ± 0.63
5.388LeuLys: 5.388 ± 0.505
5.097LeuLeu: 5.097 ± 0.614
2.621LeuMet: 2.621 ± 0.647
3.277LeuAsn: 3.277 ± 0.41
3.349LeuPro: 3.349 ± 0.489
3.786LeuGln: 3.786 ± 0.529
4.223LeuArg: 4.223 ± 0.656
4.66LeuSer: 4.66 ± 0.783
5.242LeuThr: 5.242 ± 0.602
5.242LeuVal: 5.242 ± 0.567
1.019LeuTrp: 1.019 ± 0.249
2.548LeuTyr: 2.548 ± 0.467
0.0LeuXaa: 0.0 ± 0.0
Met
3.422MetAla: 3.422 ± 0.358
0.146MetCys: 0.146 ± 0.103
1.238MetAsp: 1.238 ± 0.292
1.82MetGlu: 1.82 ± 0.352
0.947MetPhe: 0.947 ± 0.338
1.529MetGly: 1.529 ± 0.43
0.728MetHis: 0.728 ± 0.246
1.311MetIle: 1.311 ± 0.333
1.82MetLys: 1.82 ± 0.396
2.985MetLeu: 2.985 ± 0.457
0.801MetMet: 0.801 ± 0.216
1.238MetAsn: 1.238 ± 0.288
1.383MetPro: 1.383 ± 0.323
1.602MetGln: 1.602 ± 0.366
2.257MetArg: 2.257 ± 0.441
2.403MetSer: 2.403 ± 0.397
2.112MetThr: 2.112 ± 0.34
1.966MetVal: 1.966 ± 0.358
0.364MetTrp: 0.364 ± 0.148
0.801MetTyr: 0.801 ± 0.246
0.0MetXaa: 0.0 ± 0.0
Asn
3.058AsnAla: 3.058 ± 0.557
0.364AsnCys: 0.364 ± 0.168
2.112AsnAsp: 2.112 ± 0.309
2.403AsnGlu: 2.403 ± 0.447
1.602AsnPhe: 1.602 ± 0.27
3.277AsnGly: 3.277 ± 0.512
0.51AsnHis: 0.51 ± 0.215
1.893AsnIle: 1.893 ± 0.314
3.204AsnLys: 3.204 ± 0.504
3.204AsnLeu: 3.204 ± 0.495
1.092AsnMet: 1.092 ± 0.416
2.039AsnAsn: 2.039 ± 0.556
1.966AsnPro: 1.966 ± 0.369
1.456AsnGln: 1.456 ± 0.456
2.84AsnArg: 2.84 ± 0.45
2.403AsnSer: 2.403 ± 0.599
2.33AsnThr: 2.33 ± 0.444
3.058AsnVal: 3.058 ± 0.543
0.51AsnTrp: 0.51 ± 0.213
1.529AsnTyr: 1.529 ± 0.452
0.0AsnXaa: 0.0 ± 0.0
Pro
2.84ProAla: 2.84 ± 0.509
0.582ProCys: 0.582 ± 0.211
2.476ProAsp: 2.476 ± 0.479
3.058ProGlu: 3.058 ± 0.53
1.238ProPhe: 1.238 ± 0.228
1.092ProGly: 1.092 ± 0.325
0.728ProHis: 0.728 ± 0.299
1.165ProIle: 1.165 ± 0.344
2.039ProLys: 2.039 ± 0.309
2.476ProLeu: 2.476 ± 0.355
1.165ProMet: 1.165 ± 0.24
1.092ProAsn: 1.092 ± 0.39
0.874ProPro: 0.874 ± 0.175
1.747ProGln: 1.747 ± 0.365
1.238ProArg: 1.238 ± 0.257
2.403ProSer: 2.403 ± 0.382
2.257ProThr: 2.257 ± 0.427
3.422ProVal: 3.422 ± 0.558
0.874ProTrp: 0.874 ± 0.176
1.747ProTyr: 1.747 ± 0.359
0.0ProXaa: 0.0 ± 0.0
Gln
4.005GlnAla: 4.005 ± 0.479
0.655GlnCys: 0.655 ± 0.256
2.112GlnAsp: 2.112 ± 0.391
2.912GlnGlu: 2.912 ± 0.402
2.184GlnPhe: 2.184 ± 0.354
2.985GlnGly: 2.985 ± 0.407
1.019GlnHis: 1.019 ± 0.252
2.039GlnIle: 2.039 ± 0.382
1.966GlnLys: 1.966 ± 0.388
3.422GlnLeu: 3.422 ± 0.556
1.82GlnMet: 1.82 ± 0.368
1.82GlnAsn: 1.82 ± 0.397
1.092GlnPro: 1.092 ± 0.284
2.403GlnGln: 2.403 ± 0.355
2.476GlnArg: 2.476 ± 0.396
2.039GlnSer: 2.039 ± 0.419
1.893GlnThr: 1.893 ± 0.329
2.985GlnVal: 2.985 ± 0.501
0.801GlnTrp: 0.801 ± 0.233
1.602GlnTyr: 1.602 ± 0.455
0.0GlnXaa: 0.0 ± 0.0
Arg
4.878ArgAla: 4.878 ± 0.651
0.582ArgCys: 0.582 ± 0.317
2.912ArgAsp: 2.912 ± 0.466
4.514ArgGlu: 4.514 ± 0.494
2.112ArgPhe: 2.112 ± 0.386
5.024ArgGly: 5.024 ± 0.589
1.019ArgHis: 1.019 ± 0.248
3.204ArgIle: 3.204 ± 0.453
2.912ArgLys: 2.912 ± 0.443
4.005ArgLeu: 4.005 ± 0.594
2.184ArgMet: 2.184 ± 0.411
2.548ArgAsn: 2.548 ± 0.377
1.311ArgPro: 1.311 ± 0.273
2.257ArgGln: 2.257 ± 0.486
3.131ArgArg: 3.131 ± 0.47
1.747ArgSer: 1.747 ± 0.402
2.112ArgThr: 2.112 ± 0.417
3.422ArgVal: 3.422 ± 0.403
0.874ArgTrp: 0.874 ± 0.228
2.403ArgTyr: 2.403 ± 0.378
0.0ArgXaa: 0.0 ± 0.0
Ser
5.17SerAla: 5.17 ± 0.63
0.655SerCys: 0.655 ± 0.23
3.641SerAsp: 3.641 ± 0.773
3.641SerGlu: 3.641 ± 0.539
2.33SerPhe: 2.33 ± 0.452
6.335SerGly: 6.335 ± 0.732
1.311SerHis: 1.311 ± 0.284
2.767SerIle: 2.767 ± 0.669
3.859SerLys: 3.859 ± 0.624
5.097SerLeu: 5.097 ± 0.801
1.893SerMet: 1.893 ± 0.414
2.621SerAsn: 2.621 ± 0.598
1.747SerPro: 1.747 ± 0.286
2.257SerGln: 2.257 ± 0.376
3.422SerArg: 3.422 ± 0.448
3.713SerSer: 3.713 ± 0.745
3.349SerThr: 3.349 ± 0.683
4.223SerVal: 4.223 ± 0.518
0.728SerTrp: 0.728 ± 0.185
2.112SerTyr: 2.112 ± 0.551
0.0SerXaa: 0.0 ± 0.0
Thr
3.641ThrAla: 3.641 ± 0.51
0.728ThrCys: 0.728 ± 0.247
3.349ThrAsp: 3.349 ± 0.634
4.296ThrGlu: 4.296 ± 0.486
2.548ThrPhe: 2.548 ± 0.407
4.223ThrGly: 4.223 ± 0.566
1.238ThrHis: 1.238 ± 0.337
3.204ThrIle: 3.204 ± 0.558
4.005ThrLys: 4.005 ± 0.489
4.66ThrLeu: 4.66 ± 0.641
0.874ThrMet: 0.874 ± 0.225
2.694ThrAsn: 2.694 ± 0.551
2.403ThrPro: 2.403 ± 0.33
1.893ThrGln: 1.893 ± 0.422
2.33ThrArg: 2.33 ± 0.475
3.932ThrSer: 3.932 ± 0.784
3.058ThrThr: 3.058 ± 0.502
3.277ThrVal: 3.277 ± 0.604
0.582ThrTrp: 0.582 ± 0.203
1.529ThrTyr: 1.529 ± 0.351
0.0ThrXaa: 0.0 ± 0.0
Val
6.335ValAla: 6.335 ± 0.808
0.728ValCys: 0.728 ± 0.245
3.058ValAsp: 3.058 ± 0.479
4.369ValGlu: 4.369 ± 0.517
2.33ValPhe: 2.33 ± 0.363
4.077ValGly: 4.077 ± 0.664
1.383ValHis: 1.383 ± 0.359
3.786ValIle: 3.786 ± 0.536
4.951ValLys: 4.951 ± 0.602
5.024ValLeu: 5.024 ± 0.789
1.966ValMet: 1.966 ± 0.378
3.422ValAsn: 3.422 ± 0.411
3.058ValPro: 3.058 ± 0.45
3.131ValGln: 3.131 ± 0.521
3.495ValArg: 3.495 ± 0.57
5.024ValSer: 5.024 ± 0.571
4.878ValThr: 4.878 ± 0.618
3.713ValVal: 3.713 ± 0.63
0.51ValTrp: 0.51 ± 0.184
1.966ValTyr: 1.966 ± 0.385
0.0ValXaa: 0.0 ± 0.0
Trp
1.092TrpAla: 1.092 ± 0.244
0.291TrpCys: 0.291 ± 0.147
0.728TrpAsp: 0.728 ± 0.205
1.383TrpGlu: 1.383 ± 0.276
0.437TrpPhe: 0.437 ± 0.181
0.801TrpGly: 0.801 ± 0.223
0.364TrpHis: 0.364 ± 0.155
0.728TrpIle: 0.728 ± 0.206
1.238TrpLys: 1.238 ± 0.36
0.801TrpLeu: 0.801 ± 0.26
0.146TrpMet: 0.146 ± 0.134
0.364TrpAsn: 0.364 ± 0.142
1.092TrpPro: 1.092 ± 0.274
0.655TrpGln: 0.655 ± 0.205
0.655TrpArg: 0.655 ± 0.245
1.019TrpSer: 1.019 ± 0.39
0.51TrpThr: 0.51 ± 0.16
1.092TrpVal: 1.092 ± 0.256
0.073TrpTrp: 0.073 ± 0.069
0.364TrpTyr: 0.364 ± 0.129
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.131TyrAla: 3.131 ± 0.446
0.582TyrCys: 0.582 ± 0.185
1.529TyrAsp: 1.529 ± 0.295
2.184TyrGlu: 2.184 ± 0.35
1.311TyrPhe: 1.311 ± 0.282
3.277TyrGly: 3.277 ± 0.609
0.947TyrHis: 0.947 ± 0.277
1.966TyrIle: 1.966 ± 0.284
1.893TyrLys: 1.893 ± 0.351
2.912TyrLeu: 2.912 ± 0.59
1.238TyrMet: 1.238 ± 0.288
1.966TyrAsn: 1.966 ± 0.395
1.165TyrPro: 1.165 ± 0.308
1.092TyrGln: 1.092 ± 0.208
1.893TyrArg: 1.893 ± 0.354
2.403TyrSer: 2.403 ± 0.44
1.675TyrThr: 1.675 ± 0.374
1.893TyrVal: 1.893 ± 0.346
0.364TyrTrp: 0.364 ± 0.2
1.092TyrTyr: 1.092 ± 0.307
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 57 proteins (13735 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski